BrsuA.10014.s

ABC transporter (iron.B12.siderophore.hemin) , ATP-binding component

CENTER ID: BrsuA.10014.s
ORGANISM: Brucella suis 1330
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrsuA.10014.s.B1.GE36794 Full length( BrsuA.10014.s ) 1 265
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruSui107850_2966
BV-BRC: fig|204722.5.peg.2966
RefSeq: NP_699931.1
RefSeq: YP_005614438.1
UniProt: A0A0H3GBK5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MMLSARNVCW AAGCAEILHD VSLDVVKGEF LGIIGPNGSG KTTLLSLLSG IRRPRSGTVL LGNAPIQKFN RREIARRLAL VEQQAETTER ITARQAVELG RTPYLGALSP WSAADDAIVG KALENVDMAH LAGRCWHTLS GGERQRLHIA RALAQEPQIL LLDEPTNHLD IGHQIGLLDL VSRQGGLTVV AALHDLNHAA MFCDRIAVMD TGQLVALGSP REVLSAESIR AVFGVEVDVE YHGVRGCHIR YRLPDRPSLE LRCLA
NT Sequence
ATGATGCTTT CGGCACGCAA TGTTTGCTGG GCTGCGGGGT GCGCGGAAAT CCTGCATGAT GTCTCGCTCG ATGTGGTGAA GGGCGAATTT CTCGGCATTA TCGGGCCGAA CGGTTCAGGC AAGACAACCC TGCTTTCGCT GCTTTCCGGC ATCAGGCGGC CTCGCTCGGG CACAGTGCTT CTGGGCAATG CTCCGATTCA GAAGTTTAAC CGCCGCGAGA TCGCCCGACG TCTGGCGCTG GTGGAACAGC AGGCTGAAAC CACCGAGCGC ATTACGGCGC GCCAAGCCGT GGAACTGGGC CGTACACCCT ATCTGGGGGC GCTTTCGCCC TGGTCGGCGG CTGACGATGC GATTGTTGGG AAGGCGCTTG AGAACGTCGA TATGGCGCAT CTGGCCGGGC GCTGCTGGCA TACGCTTTCG GGCGGCGAGC GCCAGCGGCT CCATATCGCC CGCGCACTTG CACAGGAGCC GCAAATCCTG CTTCTGGACG AACCAACCAA CCATCTTGAT ATCGGCCACC AGATCGGGCT TCTCGATCTC GTCAGCCGTC AGGGGGGGCT GACGGTGGTG GCGGCGCTGC ACGACCTCAA CCACGCGGCC ATGTTCTGTG ATCGCATCGC GGTTATGGAC ACGGGGCAGC TCGTGGCGCT TGGGTCCCCG CGTGAGGTGT TGAGTGCTGA GTCTATTCGC GCCGTGTTCG GCGTGGAGGT CGATGTCGAA TATCACGGTG TTCGCGGCTG CCATATCCGC TATCGCTTAC CAGACAGGCC ATCCCTAGAA CTGAGGTGTT TGGCA
Details for BrsuA.10014.s.B1.GE36794
HARVESTED ON: 3/27/2013
SEQUENCED ON: 3/30/2013
EXPECTED MW: 30kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 98
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMM LSARNVCWAA GCAEILHDVS LDVVKGEFLG IIGPNGSGKT TLLSLLSGIR RPRSGTVLLG NAPIQKFNRR EIARRLALVE QQAETTERIT ARQAVELGRT PYLGALSPWS AADDAIVGKA LENVDMAHLA GRCWHTLSGG ERQRLHIARA LAQEPQILLL DEPTNHLDIG HQIGLLDLVS RQGGLTVVAA LHDLNHAAMF CDRIAVMDTG QLVALGSPRE VLSAESIRAV FGVXVDVEYH GVRGCXIRYR LXDRPSLELX CLA
Validated NT Sequence
antttnntta ctttaagaag gaganatacc atggctcacc accaccacca ccatatgatg ctttcggcac gcaatgtttg ctgggctgcg gggtgcgcgg aaatcctgca tgatgtctcg ctcgatgtgg tgaagggcga atttctcggc attatcgggc cgaacggttc aggcaagaca accctgcttt cgctgctttc cggcatcagg cggcctcgct cgggcacagt gcttctgggc aatgctccga ttcagaagtt taaccgccgc gagatcgccc gacgtctggc gctggtggaa cagcaggctg aaaccaccga gcgcattacg gcgcgccaag ccgtggaact gggccgtaca ccctatctgg gggcgctttc gccctggtcg gcggctgacg atgcgattgt tgggaaggcg cttgagaacg tcgatatggc gcatctggcc gggcgctgct ggcatacgct ttcgggcggc gagcgccagc ggctccatat cgcccgcgca cttgcacagg agccgcaaat cctgcttctg gacgaaccaa ccaaccatct tgatatcggc caccagatcg ggcttctcga tctcgtcagc cgtcaggggg ggctgacggt ggtggcggcg ctgcacgacc tcaaccacgc ggccatgttc tgtgatcgca tcgcggttat ggacacgggg cagctcgtgg cgcttgggtc cccgcgtgag gtgttgagtg ctgagtctat tcgcgccgtg ttcggcgtgg angtcgatgt cgaatatcac ggtgttcgcg gctgcnntat ccgctatcgc ttancagaca ggccatccct agaactgang tgtttggcat aagtgagtaa gataggatcc ggctgctana nncccgaang nnnnnnnann nnnnaannnn ccccnnnnnn cntccncnnn nnnnnntntn cncnnnnnnc nncnnnnnnc nnnnncnnnn ntnnnacnnn nnnnnnncnn nnnnnnttnn nanttnnnnn nnnnnnnnnn nnnnntnnnn nnnnnnnnnn nnnnctccnn nncnncnnnn nnnnnnncnc nnnnncnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncn nnnncnnnnn nncnnnnnnn nnnnnncnnc cnnnnnnnnn nnnnnnnnnn nnnnntnnnn nnncncnnnn nccnncncan nnnnnnnnnn nnnnnnnnnn t
Expected Protein Sequence
MAHHHHHHMM LSARNVCWAA GCAEILHDVS LDVVKGEFLG IIGPNGSGKT TLLSLLSGIR RPRSGTVLLG NAPIQKFNRR EIARRLALVE QQAETTERIT ARQAVELGRT PYLGALSPWS AADDAIVGKA LENVDMAHLA GRCWHTLSGG ERQRLHIARA LAQEPQILLL DEPTNHLDIG HQIGLLDLVS RQGGLTVVAA LHDLNHAAMF CDRIAVMDTG QLVALGSPRE VLSAESIRAV FGVEVDVEYH GVRGCHIRYR LPDRPSLELR CLA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgatgc tttcggcacg caatgtttgc tgggctgcgg ggtgcgcgga aatcctgcat gatgtctcgc tcgatgtggt gaagggcgaa tttctcggca ttatcgggcc gaacggttca ggcaagacaa ccctgctttc gctgctttcc ggcatcaggc ggcctcgctc gggcacagtg cttctgggca atgctccgat tcagaagttt aaccgccgcg agatcgcccg acgtctggcg ctggtggaac agcaggctga aaccaccgag cgcattacgg cgcgccaagc cgtggaactg ggccgtacac cctatctggg ggcgctttcg ccctggtcgg cggctgacga tgcgattgtt gggaaggcgc ttgagaacgt cgatatggcg catctggccg ggcgctgctg gcatacgctt tcgggcggcg agcgccagcg gctccatatc gcccgcgcac ttgcacagga gccgcaaatc ctgcttctgg acgaaccaac caaccatctt gatatcggcc accagatcgg gcttctcgat ctcgtcagcc gtcagggggg gctgacggtg gtggcggcgc tgcacgacct caaccacgcg gccatgttct gtgatcgcat cgcggttatg gacacggggc agctcgtggc gcttgggtcc ccgcgtgagg tgttgagtgc tgagtctatt cgcgccgtgt tcggcgtgga ggtcgatgtc gaatatcacg gtgttcgcgg ctgccatatc cgctatcgct taccagacag gccatcccta gaactgaggt gtttggcatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat