SSGCID: Target Page

BrsuA.10014.r

N-Acetyl-D-glucosamine ABC transport system ATP-binding protein

CENTER ID:	BrsuA.10014.r
ORGANISM:	Brucella suis 1330
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrsuA.10014.r.B1.GE36793	Full length( BrsuA.10014.r )		1	318

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	PATRIC_Locus_Tag:VBIBruSui107850_2957
BV-BRC:	fig\|204722.5.peg.2957
RefSeq:	NP_699922.1
RefSeq:	YP_005614429.1
UniProt:	Q8FVT0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLVGPSGCGK STLLRMVAGL EPISGGDLVI GGERANELPP QKRNIAMVFQ SYALFPHMTA RENIGFGPRI RGEKAAETAA KVDHAASILN LHSYLDRYPR QLSGGQRQRV AMGRAIVREP SVFLFDEPLS NLDAQLRVQM RTEIKALHQR LKSTVIYVTH DQIEAMTMAD RIVVMNQGKI QQIGAPLDLY DRPANKFVAG FIGSPSMSFI PGTVADGFFC TGEGKKIAVS AAAKGARAAE AGIRPENFVI AREGAGLTLV VEVIEPTGPE THIYGRIAGE PVRAVFRERI QLAPGEQVPV TAGSEHIHLF DKESGLPL

NT Sequence

GTGCTTGTCG GCCCGTCCGG TTGCGGAAAA TCCACGCTTT TGCGCATGGT GGCGGGGCTG GAGCCGATTT CCGGCGGCGA TCTGGTCATT GGCGGCGAGC GCGCCAACGA ATTGCCGCCG CAAAAACGCA ATATTGCGAT GGTGTTCCAG TCCTATGCGC TGTTTCCACA TATGACCGCG CGCGAGAATA TTGGTTTCGG CCCCCGTATT CGCGGCGAGA AAGCGGCGGA AACTGCGGCC AAGGTGGATC ATGCGGCCTC TATTCTCAAT CTCCATTCCT ATCTTGACCG ATATCCGCGC CAGCTTTCGG GCGGGCAGCG CCAGCGCGTG GCCATGGGCC GCGCCATCGT GCGCGAGCCT TCGGTATTTC TCTTTGACGA GCCGCTTTCC AATCTCGATG CGCAATTGCG CGTCCAGATG CGCACCGAAA TCAAGGCGCT GCATCAGCGC CTGAAATCGA CCGTGATCTA TGTCACGCAT GACCAGATCG AGGCCATGAC CATGGCCGAC CGCATCGTGG TGATGAATCA GGGCAAGATC CAGCAGATAG GCGCGCCGCT CGACCTCTAT GATCGCCCAG CCAATAAATT TGTGGCGGGC TTTATCGGCT CGCCATCGAT GAGCTTCATT CCCGGCACGG TGGCCGATGG CTTTTTCTGT ACGGGCGAGG GCAAAAAAAT TGCCGTTTCG GCGGCCGCAA AGGGCGCACG CGCGGCGGAG GCGGGCATTC GCCCGGAAAA TTTCGTTATC GCGCGCGAAG GCGCAGGCCT GACGCTTGTG GTGGAAGTGA TCGAGCCGAC CGGCCCGGAA ACCCATATCT ATGGGCGCAT TGCGGGAGAA CCCGTCCGCG CCGTGTTCCG CGAGCGGATA CAGCTTGCGC CGGGCGAGCA GGTTCCTGTA ACGGCTGGGA GCGAACATAT TCATCTCTTT GATAAGGAAA GCGGTTTGCC GCTT

Details for BrsuA.10014.r.B1.GE36793

HARVESTED ON:	3/27/2013
SEQUENCED ON:	3/30/2013
EXPECTED MW:	35kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	96
PERCENT COVERAGE:	117

Validated AA Sequence

MAHHHHHHMV LVGPSGCGKS TLLRMVAGLE PISGGDLVIG GERANELPPQ KRNIAMVFQS YALFPHMTAR ENIGFGPRIR GEKAAETAAK VDHAASILNL HSYLDRYPRQ LSGGQRQRVA MGRAIVREPS VFLFDEPLSN LDAQLRVQMR TEIKALHQRL KSTVIYVTHD QIEAMTMADR IVVMNQGKIQ QIGAPLDLYD RPANKFVAGF IGSPSMSFIP GTVADGFFCT GEGKKIAVSA AAKGARAAXA GIRPENFVIA REGAXLTLVV EVIEPTGPET HIYGRIAGEP VRAVFRERIQ LAPGEXVPVT AGXEXXHXFD XXXXXPLXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XRXXXXXXXX XXXXXXXXXX XXX

Validated NT Sequence

anattttnnt tactttaaga aggaganata ccatggctca ccaccaccac caccatatgg tgcttgtcgg cccgtccggt tgcggaaaat ccacgctttt gcgcatggtg gcggggctgg agccgatttc cggcggcgat ctggtcattg gcggcgagcg cgccaacgaa ttgccgccgc aaaaacgcaa tattgcgatg gtgttccagt cctatgcgct gtttccacat atgaccgcgc gcgagaatat tggtttcggc ccccgtattc gcggcgagaa agcggcggaa actgcggcca aggtggatca tgcggcctct attctcaatc tccattccta tcttgaccga tatccgcgcc agctttcggg cgggcagcgc cagcgcgtgg ccatgggccg cgccatcgtg cgcgagcctt cggtatttct ctttgacgag ccgctttcca atctcgatgc gcaattgcgc gtccagatgc gcaccgaaat caaggcgctg catcagcgcc tgaaatcgac cgtgatctat gtcacgcatg accagatcga ggccatgacc atggccgacc gcatcgtggt gatgaatcag ggcaagatcc agcagatagg cgcgccgctc gacctctatg atcgcccagc caataaattt gtggcgggct ttatcggctc gccatcgatg agcttcattc ccggcacggt ggccgatggc tttttctgta cgggcgaggg caaaaaaatt gccgtttcgg cggccgcaaa gggcgcacgc gcggcggang cgggcattcg cccggaaaat ttcgttatcg cgcgcgaagg cgcnngcctg acgcttgtgg tggaagtgat cgagccgacc ggcccggaaa cccatatcta tgggcgcatt gcgggagaac ccgtccgcgc cgtgttccgc gagcggatac agcttgcgcc gggcgagcng gttcctgtaa cggctgggan cgaannnntt catcnctttg atnannaaan nngntngccn ctttnantgn ntaanannnn nnnnnnnnnn nnnnnnnnnn nnnngnnnnn nnnnnnnnnn nnnnncnnnn nnnnnncntn nnnnnnnnnn nnnnnnnnnc nnnnncgnnn nnnnncnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncnnn nnnttnnnnn nnnnncnnnn gg

Expected Protein Sequence

MAHHHHHHML VGPSGCGKST LLRMVAGLEP ISGGDLVIGG ERANELPPQK RNIAMVFQSY ALFPHMTARE NIGFGPRIRG EKAAETAAKV DHAASILNLH SYLDRYPRQL SGGQRQRVAM GRAIVREPSV FLFDEPLSNL DAQLRVQMRT EIKALHQRLK STVIYVTHDQ IEAMTMADRI VVMNQGKIQQ IGAPLDLYDR PANKFVAGFI GSPSMSFIPG TVADGFFCTG EGKKIAVSAA AKGARAAEAG IRPENFVIAR EGAGLTLVVE VIEPTGPETH IYGRIAGEPV RAVFRERIQL APGEQVPVTA GSEHIHLFDK ESGLPL

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgcttg tcggcccgtc cggttgcgga aaatccacgc ttttgcgcat ggtggcgggg ctggagccga tttccggcgg cgatctggtc attggcggcg agcgcgccaa cgaattgccg ccgcaaaaac gcaatattgc gatggtgttc cagtcctatg cgctgtttcc acatatgacc gcgcgcgaga atattggttt cggcccccgt attcgcggcg agaaagcggc ggaaactgcg gccaaggtgg atcatgcggc ctctattctc aatctccatt cctatcttga ccgatatccg cgccagcttt cgggcgggca gcgccagcgc gtggccatgg gccgcgccat cgtgcgcgag ccttcggtat ttctctttga cgagccgctt tccaatctcg atgcgcaatt gcgcgtccag atgcgcaccg aaatcaaggc gctgcatcag cgcctgaaat cgaccgtgat ctatgtcacg catgaccaga tcgaggccat gaccatggcc gaccgcatcg tggtgatgaa tcagggcaag atccagcaga taggcgcgcc gctcgacctc tatgatcgcc cagccaataa atttgtggcg ggctttatcg gctcgccatc gatgagcttc attcccggca cggtggccga tggctttttc tgtacgggcg agggcaaaaa aattgccgtt tcggcggccg caaagggcgc acgcgcggcg gaggcgggca ttcgcccgga aaatttcgtt atcgcgcgcg aaggcgcagg cctgacgctt gtggtggaag tgatcgagcc gaccggcccg gaaacccata tctatgggcg cattgcggga gaacccgtcc gcgccgtgtt ccgcgagcgg atacagcttg cgccgggcga gcaggttcct gtaacggctg ggagcgaaca tattcatctc tttgataagg aaagcggttt gccgctttga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at