BrsuA.10014.q

Ferric iron ABC transporter, ATP-binding protein

CENTER ID: BrsuA.10014.q
ORGANISM: Brucella suis 1330
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrsuA.10014.q.B1.GE36792 Full length( BrsuA.10014.q ) 1 353
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruSui107850_2934
BV-BRC: fig|204722.5.peg.2934
RefSeq: NP_699896.1
RefSeq: YP_005614402.1
UniProt: Q8FVV5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTAIRPGSVT FENVTKKFGN FTALPNLSLT VEPGTLVTLL GPSGCGKTTT LRLLAGLEHP TSGRILVGGK DVTNLPANER DVSMVFQSYA LFPHMTSLEN VAYGLESSGF KKNEARERAE EGLKLVGLGG MGHRLPAELS GGQQQRVAVA RALVLEPQVL LLDEPLSNLD ARLRRRVRTE IRELQQRLGF TADYVTHDQD EALAVSDTII VMKEGGIAQK GSPRDLYEAP ASAFIADFMG EANVVPCEVI SAENGEAVIR VAGLTHRVPA RNAQPRPAQL AIRPNAVTLQ PQAGGGFSGT VAHSAYLGDH IEYEIETEHG KLFIVDPAVE QSLPLQTDVS IQFKTRGLAI INQ
NT Sequence
ATGACCGCGA TCCGCCCCGG CTCCGTAACC TTTGAAAATG TCACCAAAAA ATTCGGCAAT TTCACCGCAT TGCCCAATCT GTCGCTGACG GTGGAACCCG GTACGCTGGT CACGCTGCTG GGGCCTTCCG GCTGCGGCAA GACCACGACG CTGCGCCTGC TGGCCGGTCT GGAACATCCG ACTTCGGGGC GCATTCTCGT CGGTGGCAAG GATGTGACCA ATCTGCCTGC CAATGAGCGT GACGTGTCGA TGGTCTTCCA GTCCTATGCG CTTTTTCCGC ATATGACCTC GCTTGAAAAC GTCGCCTATG GGCTTGAATC TTCCGGCTTC AAGAAGAACG AAGCGCGCGA GCGCGCGGAA GAGGGATTGA AGCTGGTGGG CCTCGGCGGG ATGGGGCATC GCTTGCCAGC CGAATTGTCT GGCGGGCAGC AGCAGCGCGT GGCAGTGGCC CGCGCGCTCG TGCTGGAGCC GCAGGTTCTG CTTCTGGATG AGCCGCTTTC CAACCTCGAT GCACGCCTTC GCCGCCGCGT GCGCACGGAA ATCCGTGAAT TGCAGCAGCG GCTTGGTTTC ACCGCCGATT ATGTGACGCA TGACCAGGAC GAGGCGCTCG CCGTTTCGGA TACGATCATT GTTATGAAAG AGGGCGGAAT TGCCCAGAAG GGCAGCCCGC GCGATCTTTA CGAGGCTCCA GCTTCGGCCT TCATCGCCGA TTTCATGGGC GAGGCCAATG TCGTGCCCTG CGAGGTGATA AGCGCGGAAA ATGGCGAGGC TGTCATCCGC GTGGCTGGCC TTACCCATCG CGTGCCAGCA CGAAATGCCC AACCCAGACC GGCACAGCTT GCCATCCGGC CCAATGCCGT CACCTTGCAG CCGCAGGCGG GTGGCGGGTT TTCCGGCACG GTCGCACATT CTGCCTATCT CGGCGATCAT ATCGAATATG AGATAGAGAC CGAACACGGA AAGCTCTTCA TCGTCGATCC GGCCGTGGAA CAGTCCTTGC CGCTCCAGAC GGATGTTTCC ATCCAGTTCA AGACACGCGG CCTTGCCATC ATCAACCAA
Details for BrsuA.10014.q.B1.GE36792
HARVESTED ON: 3/27/2013
SEQUENCED ON: 3/30/2013
EXPECTED MW: 39kDa
OBSERVED MW: 39kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 95
PERCENT COVERAGE: 88
Validated AA Sequence
MAHHHHHHMT AIRPGSVTFE NVTKKFGNFT ALPNLSLTVE PGTLVTLLGP SGCGKTTTLR LLAGLEHPTS GRILVGGKDV TNLPANERDV SMVFQSYALF PHMTSLENVA YGLESSGFKK NEARERAEEG LKLVGLGGMG HRLPAELSGG QQQRVAVARA LVLEPQVLLL DEPLSNLDAR LRRRVRTEIR ELQQRLGFTA DYVTHDQDEA LAVSDTIIVM KEGGIAQKGS PRDLYEAPAS AFIADFMGEA NVVPCEVISA ENGEAVIRVA GLTHRVPARN AQPRPAQLAI RPNAVTLQPX XVAGFXXRSH ILXIXXXISX
Validated NT Sequence
ttgnnnnntt taagaaggag anataccatg gctcaccacc accaccacca tatgaccgcg atccgccccg gctccgtaac ctttgaaaat gtcaccaaaa aattcggcaa tttcaccgca ttgcccaatc tgtcgctgac ggtggaaccc ggtacgctgg tcacgctgct ggggccttcc ggctgcggca agaccacgac gctgcgcctg ctggccggtc tggaacatcc gacttcgggg cgcattctcg tcggtggcaa ggatgtgacc aatctgcctg ccaatgagcg tgacgtgtcg atggtcttcc agtcctatgc gctttttccg catatgacct cgcttgaaaa cgtcgcctat gggcttgaat cttccggctt caagaagaac gaagcgcgcg agcgcgcgga agagggattg aagctggtgg gcctcggcgg gatggggcat cgcttgccag ccgaattgtc tggcgggcag cagcagcgcg tggcagtggc ccgcgcgctc gtgctggagc cgcaggttct gcttctggat gagccgcttt ccaacctcga tgcacgcctt cgccgccgcg tgcgcacgga aatccgtgaa ttgcagcagc ggcttggttt caccgccgat tatgtgacgc atgaccagga cgaggcgctc gccgtttcgg atacgatcat tgttatgaaa gagggcggaa ttgcccagaa gggcagcccg cgcgatcttt acgaggctcc agcttcggcc ttcatcgccg atttcatggg cgaggccaat gtcgtgccct gcgaggtgat aagcgcggaa aatggcgagg ctgtcatccg cgtggctggc cttacccatc gcgtgccagc acgaaatgcc caacccagac cggcacagct tgccatccgg cccaatgccg tcaccttgca gccgcnnngg gtggcgggtt ttnnnncacg gtcgcacatt ctgnctatcn cgncgancat atcnanatga natnnaganc gaannnnnna nnntnttcnn cgtcnannnn nnnnnngtcn nnnnnnnnnc nnannnngtt nnnncnnnnn nnanncnnnn nncnnnnnna cnnannnnnn a
Expected Protein Sequence
MAHHHHHHMT AIRPGSVTFE NVTKKFGNFT ALPNLSLTVE PGTLVTLLGP SGCGKTTTLR LLAGLEHPTS GRILVGGKDV TNLPANERDV SMVFQSYALF PHMTSLENVA YGLESSGFKK NEARERAEEG LKLVGLGGMG HRLPAELSGG QQQRVAVARA LVLEPQVLLL DEPLSNLDAR LRRRVRTEIR ELQQRLGFTA DYVTHDQDEA LAVSDTIIVM KEGGIAQKGS PRDLYEAPAS AFIADFMGEA NVVPCEVISA ENGEAVIRVA GLTHRVPARN AQPRPAQLAI RPNAVTLQPQ AGGGFSGTVA HSAYLGDHIE YEIETEHGKL FIVDPAVEQS LPLQTDVSIQ FKTRGLAIIN Q
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaccg cgatccgccc cggctccgta acctttgaaa atgtcaccaa aaaattcggc aatttcaccg cattgcccaa tctgtcgctg acggtggaac ccggtacgct ggtcacgctg ctggggcctt ccggctgcgg caagaccacg acgctgcgcc tgctggccgg tctggaacat ccgacttcgg ggcgcattct cgtcggtggc aaggatgtga ccaatctgcc tgccaatgag cgtgacgtgt cgatggtctt ccagtcctat gcgctttttc cgcatatgac ctcgcttgaa aacgtcgcct atgggcttga atcttccggc ttcaagaaga acgaagcgcg cgagcgcgcg gaagagggat tgaagctggt gggcctcggc gggatggggc atcgcttgcc agccgaattg tctggcgggc agcagcagcg cgtggcagtg gcccgcgcgc tcgtgctgga gccgcaggtt ctgcttctgg atgagccgct ttccaacctc gatgcacgcc ttcgccgccg cgtgcgcacg gaaatccgtg aattgcagca gcggcttggt ttcaccgccg attatgtgac gcatgaccag gacgaggcgc tcgccgtttc ggatacgatc attgttatga aagagggcgg aattgcccag aagggcagcc cgcgcgatct ttacgaggct ccagcttcgg ccttcatcgc cgatttcatg ggcgaggcca atgtcgtgcc ctgcgaggtg ataagcgcgg aaaatggcga ggctgtcatc cgcgtggctg gccttaccca tcgcgtgcca gcacgaaatg cccaacccag accggcacag cttgccatcc ggcccaatgc cgtcaccttg cagccgcagg cgggtggcgg gttttccggc acggtcgcac attctgccta tctcggcgat catatcgaat atgagataga gaccgaacac ggaaagctct tcatcgtcga tccggccgtg gaacagtcct tgccgctcca gacggatgtt tccatccagt tcaagacacg cggccttgcc atcatcaacc aatgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat