SSGCID: Target Page

BrsuA.10014.p

ABC-type enterochelin transport system, ATPase component

CENTER ID:	BrsuA.10014.p
ORGANISM:	Brucella suis 1330
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrsuA.10014.p.B1.GE36791	Full length( BrsuA.10014.p )		1	252

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	PATRIC_Locus_Tag:VBIBruSui107850_2890
BV-BRC:	fig\|204722.5.peg.2890
RefSeq:	NP_699860.1
RefSeq:	YP_005614366.1
UniProt:	A0A0H3GBE8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MIEISQVSKS YNGTLVVDDV TLRLPARGIT SIIGPNGAGK STLLSMVSRL LPMDKGSVTV DGLDITTTSG DVLARRLSIL RQENAINSRL TVRDLVTFGR YPYSKGRPNS EDRKHVEQAI GYLGLEGLSE RFLDELSGGQ RQRAFVAMVL CQDTDYVLLD EPLNNLDMKH SASMMKLLRR AADELGKTIV LVLHDINFAS CYSDHIVAMR HGKVVHQGSP DELIHPEILS EIYEMEIGVE IIGGKRIGVY YL

NT Sequence

TTGATCGAAA TAAGCCAGGT CAGCAAATCC TATAACGGCA CACTCGTCGT CGATGATGTA ACGCTGCGCT TGCCCGCACG CGGAATCACA TCGATCATCG GGCCCAATGG CGCCGGAAAG TCCACCCTGC TTTCAATGGT GAGCCGCCTC CTGCCCATGG ACAAGGGCAG CGTGACCGTG GACGGGCTGG ATATCACCAC CACGTCGGGT GATGTGCTGG CCAGGCGGCT TTCCATCCTG CGGCAGGAAA ACGCCATCAA TTCGCGCCTC ACCGTGCGCG ATCTCGTGAC TTTCGGGCGC TATCCCTATT CCAAAGGACG TCCCAACAGC GAAGACCGCA AGCATGTGGA ACAGGCTATC GGCTATCTTG GCCTTGAAGG TTTGAGCGAA CGTTTTCTGG ACGAGCTTTC CGGCGGCCAG CGCCAGCGCG CCTTTGTGGC GATGGTGCTG TGCCAGGATA CGGATTACGT TCTGCTGGAC GAACCGCTCA ACAATCTCGA TATGAAGCAT TCCGCCTCGA TGATGAAGCT TCTGCGCCGC GCTGCCGATG AACTGGGAAA GACCATCGTT CTCGTGCTGC ACGACATCAA TTTTGCGTCG TGCTATTCAG ATCATATCGT TGCCATGCGC CACGGCAAGG TCGTGCATCA GGGAAGCCCG GATGAACTCA TCCATCCGGA AATTCTCAGC GAAATCTATG AGATGGAAAT CGGCGTCGAA ATCATCGGCG GCAAGCGCAT AGGCGTCTAT TATCTC

Details for BrsuA.10014.p.B1.GE36791

HARVESTED ON:	3/27/2013
SEQUENCED ON:	3/30/2013
EXPECTED MW:	29kDa
OBSERVED MW:	29kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHML IEISQVSKSY NGTLVVDDVT LRLPARGITS IIGPNGAGKS TLLSMVSRLL PMDKGSVTVD GLDITTTSGD VLARRLSILR QENAINSRLT VRDLVTFGRY PYSKGRPNSE DRKHVEQAIG YLGLEGLSER FLDELSGGQR QRAFVAMVLC QDTDYVLLDE PLNNLDMKHS ASMMKLLRRA ADELGKTIVL VLHDINFASC YSDHIVAMRH GKVVHQGSPD ELIHPEILSE IYEMEIGVEI IGGKRIGVYY L

Validated NT Sequence

tcttactcac ttagagataa tagacgccta tgcgcttgcc gccgatgatt tcgacgccga tttccatctc atagatttcg ctgagaattt ccggatggat gagttcatcc gggcttccct gatgcacgac cttgccgtgg cgcatggcaa cgatatgatc tgaatagcac gacgcaaaat tgatgtcgtg cagcacgaga acgatggtct ttcccagttc atcggcagcg cggcgcagaa gcttcatcat cgaggcggaa tgcttcatat cgagattgtt gagcggttcg tccagcagaa cgtaatccgt atcctggcac agcaccatcg ccacaaaggc gcgctggcgc tggccgccgg aaagctcgtc cagaaaacgt tcgctcaaac cttcaaggcc aagatagccg atagcctgtt ccacatgctt gcggtcttcg ctgttgggac gtcctttgga atagggatag cgcccgaaag tcacgagatc gcgcacggtg aggcgcgaat tgatggcgtt ttcctgccgc aggatggaaa gccgcctggc cagcacatca cccgacgtgg tggtgatatc cagcccgtcc acggtcacgc tgcccttgtc catgggcagg aggcggctca ccattgaaag cagggtggac tttccggcgc cattgggccc gatgatcgat gtgattccgc gtgcgggcaa gcgcagcgtt acatcatcga cgacgagtgt gccgttatag gatttgctga cctggcttat ttcgatcaac atatggtggt ggtggtggtg agccat

Expected Protein Sequence

MAHHHHHHMI EISQVSKSYN GTLVVDDVTL RLPARGITSI IGPNGAGKST LLSMVSRLLP MDKGSVTVDG LDITTTSGDV LARRLSILRQ ENAINSRLTV RDLVTFGRYP YSKGRPNSED RKHVEQAIGY LGLEGLSERF LDELSGGQRQ RAFVAMVLCQ DTDYVLLDEP LNNLDMKHSA SMMKLLRRAA DELGKTIVLV LHDINFASCY SDHIVAMRHG KVVHQGSPDE LIHPEILSEI YEMEIGVEII GGKRIGVYYL

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttgatcg aaataagcca ggtcagcaaa tcctataacg gcacactcgt cgtcgatgat gtaacgctgc gcttgcccgc acgcggaatc acatcgatca tcgggcccaa tggcgccgga aagtccaccc tgctttcaat ggtgagccgc ctcctgccca tggacaaggg cagcgtgacc gtggacgggc tggatatcac caccacgtcg ggtgatgtgc tggccaggcg gctttccatc ctgcggcagg aaaacgccat caattcgcgc ctcaccgtgc gcgatctcgt gactttcggg cgctatccct attccaaagg acgtcccaac agcgaagacc gcaagcatgt ggaacaggct atcggctatc ttggccttga aggtttgagc gaacgttttc tggacgagct ttccggcggc cagcgccagc gcgcctttgt ggcgatggtg ctgtgccagg atacggatta cgttctgctg gacgaaccgc tcaacaatct cgatatgaag cattccgcct cgatgatgaa gcttctgcgc cgcgctgccg atgaactggg aaagaccatc gttctcgtgc tgcacgacat caattttgcg tcgtgctatt cagatcatat cgttgccatg cgccacggca aggtcgtgca tcagggaagc ccggatgaac tcatccatcc ggaaattctc agcgaaatct atgagatgga aatcggcgtc gaaatcatcg gcggcaagcg cataggcgtc tattatctct gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat