SSGCID: Target Page

BrsuA.10014.n

Nitrous oxide reductase maturation protein NosF (ATPase)

CENTER ID:	BrsuA.10014.n
ORGANISM:	Brucella suis 1330
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrsuA.10014.n.B1.GE36789	Full length( BrsuA.10014.n )		1	331

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	PATRIC_Locus_Tag:VBIBruSui107850_2494
BV-BRC:	fig\|204722.5.peg.2494
RefSeq:	NP_699473.1
RefSeq:	YP_005613982.1
UniProt:	A0A0H3GA13

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTEHRNAEKR HLQTGMATFG TGSETVVLDK VTKSYGGFNA ISDASFRLMA GENIALVGHN GAGKTTMIKL MLGLIRPTKG AVRVLGEHPA AGAFAARLRL GYLPEHVSFN MALTGAETLG FYARLKQVPF GQADALLERV GLAHAAHRRV GTYSKGMRQR LGLAQALLGQ PAVLLLDEPT TGLDPALRQS FYEILESLRQ EGTTILMSSH ALTELETKVG RVIIANKGKV IADGSIDALR RISRLPMRIR VEVNDVSCLP VETGNLLLRW HRLGPDLYET EAPAEGKMEL VRRIVAMNDK LNELDIIPPT LDELYAHFLN MGDMAVQEAA E

NT Sequence

ATGACTGAGC ATCGGAACGC AGAAAAGCGC CATTTGCAGA CCGGCATGGC GACTTTTGGA ACAGGCTCCG AGACTGTTGT TCTTGATAAG GTTACCAAGT CCTATGGCGG CTTTAACGCC ATAAGCGATG CAAGTTTCCG GCTTATGGCG GGGGAAAATA TTGCACTTGT CGGCCATAAT GGCGCGGGCA AGACCACGAT GATCAAGCTC ATGCTTGGCC TGATCCGCCC CACCAAAGGC GCGGTGCGTG TGCTGGGTGA ACACCCTGCG GCGGGTGCCT TTGCCGCGCG GTTGCGGCTT GGCTACCTGC CCGAACATGT CTCGTTCAAC ATGGCGCTGA CGGGCGCGGA GACACTTGGC TTCTATGCCC GGCTGAAACA GGTGCCCTTT GGCCAGGCGG ATGCACTTCT GGAACGTGTC GGCCTTGCCC ATGCTGCCCA TCGTCGCGTC GGCACCTATT CCAAGGGGAT GCGCCAACGC CTCGGACTTG CGCAGGCTTT GCTGGGGCAG CCCGCCGTGC TTTTGCTTGA CGAACCGACC ACGGGTCTCG ATCCGGCCCT GCGCCAGAGT TTCTATGAAA TTCTGGAAAG CTTGCGGCAG GAGGGCACGA CCATTCTCAT GTCCTCCCAT GCGCTGACCG AGCTTGAAAC TAAGGTCGGG CGCGTCATCA TCGCCAATAA AGGTAAGGTG ATCGCGGATG GTTCCATCGA CGCGCTGCGC CGGATTTCAC GCCTGCCGAT GCGTATCCGT GTGGAGGTGA ACGACGTTTC ATGCCTGCCG GTCGAAACCG GCAACCTGCT GTTGCGCTGG CATCGGCTCG GTCCCGATCT TTACGAAACG GAAGCCCCGG CGGAGGGCAA GATGGAACTT GTACGCCGCA TCGTGGCCAT GAACGACAAG CTGAACGAAC TCGATATCAT CCCGCCCACG CTGGACGAGC TTTACGCGCA TTTTCTCAAT ATGGGCGACA TGGCGGTTCA GGAGGCGGCA GAA

Details for BrsuA.10014.n.B1.GE36789

HARVESTED ON:	3/27/2013
SEQUENCED ON:	3/30/2013
EXPECTED MW:	37kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	92
PERCENT COVERAGE:	103

Validated AA Sequence

MAHHHHHHMT EHRNAEKRHL QTGMATFGTG SETVVLDKVT KSYGGFNAIS DASFRLMAGE NIALVGHNGA GKTTMIKLML GLIRPTKGAV RVLGEHPAAG AFAARLRLGY LPEHVSFNMA LTGAETLGFY ARLKQVPFGQ ADALLERVGL AHAAHRRVGT YSKGMRQRLG LAQALLGQPA VLLLDEPTTG LDPALRQSFY EILESLRQEG TTILMSSHAL TELETKVGRV IIANKGKVIA DGSIDALRRI SRLPMRIRVE VNDVSCLPVE TGNLLLRWHR LGPDLYXTEA PAXGKMELVR XIVAMNDKLN ELDIIPPXWT XFTRIFSXWX XXXFXXXXXX XXXXXXXXXX

Validated NT Sequence

cnnnnnnnnn nntttgnnnn ntttaagaag gagatatacc atggctcacc accaccacca ccatatgact gagcatcgga acgcagaaaa gcgccatttg cagaccggca tggcgacttt tggaacaggc tccgagactg ttgttcttga taaggttacc aagtcctatg gcggctttaa cgccataagc gatgcaagtt tccggcttat ggcgggggaa aatattgcac ttgtcggcca taatggcgcg ggcaagacca cgatgatcaa gctcatgctt ggcctgatcc gccccaccaa aggcgcggtg cgtgtgctgg gtgaacaccc tgcggcgggt gcctttgccg cgcggttgcg gcttggctac ctgcccgaac atgtctcgtt caacatggcg ctgacgggcg cggagacact tggcttctat gcccggctga aacaggtgcc ctttggccag gcggatgcac ttctggaacg tgtcggcctt gcccatgctg cccatcgtcg cgtcggcacc tattccaagg ggatgcgcca acgcctcgga cttgcgcagg ctttgctggg gcagcccgcc gtgcttttgc ttgacgaacc gaccacgggt ctcgatccgg ccctgcgcca gagtttctat gaaattctgg aaagcttgcg gcaggagggc acgaccattc tcatgtcctc ccatgcgctg accgagcttg aaactaaggt cgggcgcgtc atcatcgcca ataaaggtaa ggtgatcgcg gatggttcca tcgacgcgct gcgccggatt tcacgcctgc cgatgcgtat ccgtgtggag gtgaacgacg tttcatgcct gccggtcgaa accggcaacc tgctgttgcg ctggcatcgg ctcggtcccg atctttacna aacggaagcc ccggcggang gcaagatgga acttgtacgc cncatcgtgg ccatgaacga caagctgaac gaactcgata tcatcccgcc nnnctggacg anctttacgc gcattttctc annntgggnn nnntgnnngt tcnnnnnnnn nannannnnn nnnnnnnnnn ncngnnnnnn nnnnnnnnnn a

Expected Protein Sequence

MAHHHHHHMT EHRNAEKRHL QTGMATFGTG SETVVLDKVT KSYGGFNAIS DASFRLMAGE NIALVGHNGA GKTTMIKLML GLIRPTKGAV RVLGEHPAAG AFAARLRLGY LPEHVSFNMA LTGAETLGFY ARLKQVPFGQ ADALLERVGL AHAAHRRVGT YSKGMRQRLG LAQALLGQPA VLLLDEPTTG LDPALRQSFY EILESLRQEG TTILMSSHAL TELETKVGRV IIANKGKVIA DGSIDALRRI SRLPMRIRVE VNDVSCLPVE TGNLLLRWHR LGPDLYETEA PAEGKMELVR RIVAMNDKLN ELDIIPPTLD ELYAHFLNMG DMAVQEAAE

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgactg agcatcggaa cgcagaaaag cgccatttgc agaccggcat ggcgactttt ggaacaggct ccgagactgt tgttcttgat aaggttacca agtcctatgg cggctttaac gccataagcg atgcaagttt ccggcttatg gcgggggaaa atattgcact tgtcggccat aatggcgcgg gcaagaccac gatgatcaag ctcatgcttg gcctgatccg ccccaccaaa ggcgcggtgc gtgtgctggg tgaacaccct gcggcgggtg cctttgccgc gcggttgcgg cttggctacc tgcccgaaca tgtctcgttc aacatggcgc tgacgggcgc ggagacactt ggcttctatg cccggctgaa acaggtgccc tttggccagg cggatgcact tctggaacgt gtcggccttg cccatgctgc ccatcgtcgc gtcggcacct attccaaggg gatgcgccaa cgcctcggac ttgcgcaggc tttgctgggg cagcccgccg tgcttttgct tgacgaaccg accacgggtc tcgatccggc cctgcgccag agtttctatg aaattctgga aagcttgcgg caggagggca cgaccattct catgtcctcc catgcgctga ccgagcttga aactaaggtc gggcgcgtca tcatcgccaa taaaggtaag gtgatcgcgg atggttccat cgacgcgctg cgccggattt cacgcctgcc gatgcgtatc cgtgtggagg tgaacgacgt ttcatgcctg ccggtcgaaa ccggcaacct gctgttgcgc tggcatcggc tcggtcccga tctttacgaa acggaagccc cggcggaggg caagatggaa cttgtacgcc gcatcgtggc catgaacgac aagctgaacg aactcgatat catcccgccc acgctggacg agctttacgc gcattttctc aatatgggcg acatggcggt tcaggaggcg gcagaatgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t