SSGCID: Target Page

BrsuA.10014.m

Molybdenum transport ATP-binding protein ModC (TC 3.A.1.8.1)

CENTER ID:	BrsuA.10014.m
ORGANISM:	Brucella suis 1330
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrsuA.10014.m.B1.GE36788	Full length( BrsuA.10014.m )		1	359

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	PATRIC_Locus_Tag:VBIBruSui107850_2311
BV-BRC:	fig\|204722.5.peg.2311
RefSeq:	NP_699295.1
RefSeq:	YP_005613806.1
UniProt:	Q8FXI7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSGLTVSIRG RNGAFAIEAG FAAEGGVTAL FGHSGAGKTT LLKMIAGTLR PENGRIAVGD FTLFDAQKGI NLPPEKRCIG YVFQDARLFA YMSVKRNLTY ARWAGHRQAT RSFDEVVALL GIGHLLDRRP STLSGGERQR VAIGRALLSD PALLLLDEPL SSLDHARRQE ILPFIERLRD ESHVPIVYVS HEIDEVARLA DQIVLLSAGR VTASGAAADI FPLIDAESEG GGVLLEGIVS AYDERYKLAE IDLGGASFQL SDAGLKQTMH VRLRVRARDV SIARKIPEAI SIRNLLPVTV TGIERGEGPN AHVFLDFRGR RLGARLTRRS VDDLGLSVGD QVVALVKAVS VDRAAIREK

NT Sequence

ATGAGCGGGC TTACGGTTTC CATCAGGGGC CGCAACGGGG CCTTTGCCAT TGAGGCCGGT TTTGCCGCTG AGGGGGGCGT CACGGCTTTG TTCGGCCATT CGGGTGCGGG CAAGACGACG CTTTTGAAAA TGATCGCCGG AACGCTCCGC CCGGAAAACG GACGCATCGC GGTTGGCGAT TTTACGCTGT TCGATGCGCA AAAAGGCATA AACCTGCCGC CGGAAAAGCG CTGCATCGGC TATGTTTTTC AGGATGCGCG ATTGTTTGCG TATATGAGCG TCAAGCGCAA CCTTACCTAT GCCCGCTGGG CAGGCCACAG GCAGGCCACG CGCAGCTTCG ATGAGGTGGT GGCGCTGCTC GGCATCGGCC ATCTCCTCGA CCGTCGCCCC TCGACACTTT CCGGCGGCGA GCGCCAGCGT GTCGCCATTG GCCGCGCGCT TTTGTCCGAT CCCGCACTTC TGCTGCTCGA TGAGCCATTA TCCTCGCTCG ATCATGCGCG GCGGCAGGAA ATATTGCCCT TCATTGAGCG GCTTCGCGAC GAAAGCCATG TGCCGATCGT CTATGTGAGC CATGAGATCG ATGAGGTGGC GCGGCTTGCC GATCAGATCG TGCTGCTATC GGCTGGCCGG GTGACGGCAA GCGGGGCGGC AGCGGATATT TTCCCGCTGA TCGATGCGGA AAGCGAGGGC GGCGGCGTGC TTCTGGAAGG CATAGTCTCC GCTTATGACG AGCGTTACAA ACTGGCCGAG ATCGACCTTG GCGGCGCCTC GTTCCAGTTG AGCGATGCCG GGCTGAAGCA GACCATGCAT GTGCGCCTGC GGGTGCGCGC GCGCGATGTT TCGATTGCGC GCAAAATTCC TGAAGCGATC AGCATACGCA ACCTTCTGCC GGTTACGGTG ACGGGCATTG AAAGGGGGGA AGGGCCGAAT GCGCATGTTT TTCTTGATTT CCGCGGTCGC CGTCTCGGTG CGCGGCTGAC GCGCCGCTCG GTGGATGATC TGGGCCTGTC GGTGGGCGAT CAGGTGGTGG CTCTTGTCAA AGCCGTTTCG GTGGATCGGG CGGCAATCCG GGAAAAA

Details for BrsuA.10014.m.B1.GE36788

HARVESTED ON:	3/27/2013
SEQUENCED ON:	3/30/2013
EXPECTED MW:	40kDa
OBSERVED MW:	40kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	86
PERCENT COVERAGE:	96

Validated AA Sequence

MAHHHHHHMS GLTVSIRGRN GAFAIEAGFA AEGGVTALFG HSGAGKTTLL KMIAGTLRPE NGRIAVGDFT LFDAQKGINL PPEKRCIGYV FQDARLFAYM SVKRNLTYAR WAGHRQATRS FDEVVALLGI GHLLDRRPST LSGGERQRVA IGRALLSDPA LLLLDEPLSS LDHARRQEIL PFIERLRDES HVPIVYVSHE IDEVARLADQ IVLLSAGRVT ASGAAADIFP LIDAESEGGG VLLEGIVSAY DERYKLAEID LGGASFQLSD AGLKQXMHVR LRVRARDVSI ARKIXEAISI RNLLPVXXXX LXXGXGRMXM XXXISXXXXX XXXXXXXXXX XXXXXXXXXX XXXXX

Validated NT Sequence

cnnnnnnnat tttgnttact ttaagaagga ganataccat ggctcaccac caccaccacc atatgagcgg gcttacggtt tccatcaggg gccgcaacgg ggcctttgcc attgaggccg gttttgccgc tgaggggggc gtcacggctt tgttcggcca ttcgggtgcg ggcaagacga cgcttttgaa aatgatcgcc ggaacgctcc gcccggaaaa cggacgcatc gcggttggcg attttacgct gttcgatgcg caaaaaggca taaacctgcc gccggaaaag cgctgcatcg gctatgtttt tcaggatgcg cgattgtttg cgtatatgag cgtcaagcgc aaccttacct atgcccgctg ggcaggccac aggcaggcca cgcgcagctt cgatgaggtg gtggcgctgc tcggcatcgg ccatctcctc gaccgtcgcc cctcgacact ttccggcggc gagcgccagc gtgtcgccat tggccgcgcg cttttgtccg atcccgcact tctgctgctc gatgagccat tatcctcgct cgatcatgcg cggcggcagg aaatattgcc cttcattgag cggcttcgcg acgaaagcca tgtgccgatc gtctatgtga gccatgagat cgatgaggtg gcgcggcttg ccgatcagat cgtgctgcta tcggctggcc gggtgacggc aagcggggcg gcagcggata ttttcccgct gatcgatgcg gaaagcgagg gcggcggcgt gcttctggaa ggcatagtct ccgcttatga cgagcgttac aaactggccg agatcgacct tggcggcgcc tcgttccagt tgagcgatgc cgggctgaag cagancatgc atgtgcgcct gcgggtgcgc gcgcgcgatg tttcgattgc gcgcaaaatt nctgaagcga tcagcatacg caaccttctg ccngttannn gangggnatt gnannggggg aanggncgaa tgngcatgnt tttnntgatt tccnnngncn nccnnnnncn annnnnccnn ntnnnnngnn nnnnnngnnn nnnnnnnngn nnnnnngnnn nnnnnnnnnn anncnntnnn nnngta

Expected Protein Sequence

MAHHHHHHMS GLTVSIRGRN GAFAIEAGFA AEGGVTALFG HSGAGKTTLL KMIAGTLRPE NGRIAVGDFT LFDAQKGINL PPEKRCIGYV FQDARLFAYM SVKRNLTYAR WAGHRQATRS FDEVVALLGI GHLLDRRPST LSGGERQRVA IGRALLSDPA LLLLDEPLSS LDHARRQEIL PFIERLRDES HVPIVYVSHE IDEVARLADQ IVLLSAGRVT ASGAAADIFP LIDAESEGGG VLLEGIVSAY DERYKLAEID LGGASFQLSD AGLKQTMHVR LRVRARDVSI ARKIPEAISI RNLLPVTVTG IERGEGPNAH VFLDFRGRRL GARLTRRSVD DLGLSVGDQV VALVKAVSVD RAAIREK

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagcg ggcttacggt ttccatcagg ggccgcaacg gggcctttgc cattgaggcc ggttttgccg ctgagggggg cgtcacggct ttgttcggcc attcgggtgc gggcaagacg acgcttttga aaatgatcgc cggaacgctc cgcccggaaa acggacgcat cgcggttggc gattttacgc tgttcgatgc gcaaaaaggc ataaacctgc cgccggaaaa gcgctgcatc ggctatgttt ttcaggatgc gcgattgttt gcgtatatga gcgtcaagcg caaccttacc tatgcccgct gggcaggcca caggcaggcc acgcgcagct tcgatgaggt ggtggcgctg ctcggcatcg gccatctcct cgaccgtcgc ccctcgacac tttccggcgg cgagcgccag cgtgtcgcca ttggccgcgc gcttttgtcc gatcccgcac ttctgctgct cgatgagcca ttatcctcgc tcgatcatgc gcggcggcag gaaatattgc ccttcattga gcggcttcgc gacgaaagcc atgtgccgat cgtctatgtg agccatgaga tcgatgaggt ggcgcggctt gccgatcaga tcgtgctgct atcggctggc cgggtgacgg caagcggggc ggcagcggat attttcccgc tgatcgatgc ggaaagcgag ggcggcggcg tgcttctgga aggcatagtc tccgcttatg acgagcgtta caaactggcc gagatcgacc ttggcggcgc ctcgttccag ttgagcgatg ccgggctgaa gcagaccatg catgtgcgcc tgcgggtgcg cgcgcgcgat gtttcgattg cgcgcaaaat tcctgaagcg atcagcatac gcaaccttct gccggttacg gtgacgggca ttgaaagggg ggaagggccg aatgcgcatg tttttcttga tttccgcggt cgccgtctcg gtgcgcggct gacgcgccgc tcggtggatg atctgggcct gtcggtgggc gatcaggtgg tggctcttgt caaagccgtt tcggtggatc gggcggcaat ccgggaaaaa tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat