SSGCID: Target Page

BrsuA.10014.l

Branched-chain amino acid transport ATP-binding protein LivF (TC 3.A.1.4.1)

CENTER ID:	BrsuA.10014.l
ORGANISM:	Brucella suis 1330
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrsuA.10014.l.B1.GE36787	Full length( BrsuA.10014.l )		1	238

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	PATRIC_Locus_Tag:VBIBruSui107850_2250
BV-BRC:	fig\|204722.5.peg.2250
RefSeq:	NP_699235.1
RefSeq:	YP_005613746.1
UniProt:	A0A0H3G9D3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAAENILDVR ELHAHYGKSH ILQGVTFDLK KGEAISLLGR NGSGRSTTMK AIMGLVPPTS GEVLLNGKNL AGERSFKICR AGIGFVPEER ETFLNLTVDE NLQMGIQRGA EGATRWTVEQ MFDYFPRLKE RRNTRAGSFS GGEQQMLTMC RTLLGNPLVM LIDEPTEGLA PKIVAVVGEM IKDINRQGVS VVLVEQKLTI ALKVSSRVCV MGHGRIVFEG TPDELTADEK LMEEWLAV

NT Sequence

ATGGCTGCTG AAAACATTCT CGATGTTCGC GAATTGCACG CGCATTACGG CAAGAGCCAT ATCCTGCAAG GCGTGACCTT CGACCTGAAG AAGGGCGAGG CGATCAGCCT GCTGGGGCGC AATGGTTCGG GCCGTTCCAC CACCATGAAG GCGATCATGG GGCTGGTGCC GCCGACAAGC GGCGAAGTGC TTCTGAACGG GAAAAATCTG GCGGGCGAGC GTTCGTTCAA AATCTGCCGC GCAGGCATTG GTTTCGTACC GGAAGAGCGT GAAACCTTCC TCAATCTGAC CGTGGATGAA AACCTGCAAA TGGGTATTCA GCGCGGTGCG GAAGGGGCGA CCAGATGGAC GGTCGAACAG ATGTTCGACT ATTTCCCGCG CCTCAAGGAA CGCCGCAACA CCCGCGCGGG CAGCTTTTCG GGCGGCGAGC AGCAGATGCT GACCATGTGC CGCACATTGC TCGGCAATCC GCTGGTCATG CTGATCGACG AACCGACAGA AGGCCTTGCG CCGAAGATCG TCGCTGTCGT CGGCGAGATG ATCAAGGACA TCAACCGGCA GGGCGTTTCA GTGGTGCTGG TGGAGCAGAA GCTCACCATT GCGCTGAAAG TTTCCAGCCG CGTCTGTGTG ATGGGGCATG GCCGCATCGT TTTTGAAGGA ACGCCGGACG AACTGACCGC CGACGAAAAA CTGATGGAGG AATGGCTGGC CGTC

Details for BrsuA.10014.l.B1.GE36787

HARVESTED ON:	3/27/2013
SEQUENCED ON:	3/30/2013
EXPECTED MW:	27kDa
OBSERVED MW:	27kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMA AENILDVREL HAHYGKSHIL QGVTFDLKKG EAISLLGRNG SGRSTTMKAI MGLVPPTSGE VLLNGKNLAG ERSFKICRAG IGFVPEERET FLNLTVDENL QMGIQRGAEG ATRWTVEQMF DYFPRLKERR NTRAGSFSGG EQQMLTMCRT LLGNPLVMLI DEPTEGLAPK IVAVVGEMIK DINRQGVSVV LVEQKLTIAL KVSSRVCVMG HGRIVFEGTP DELTADEKLM EEWLAV

Validated NT Sequence

anattttnnn nnntttaaga aggaganata ccatggctca ccaccaccac caccatatgg ctgctgaaaa cattctcgat gttcgcgaat tgcacgcgca ttacggcaag agccatatcc tgcaaggcgt gaccttcgac ctgaagaagg gcgaggcgat cagcctgctg gggcgcaatg gttcgggccg ttccaccacc atgaaggcga tcatggggct ggtgccgccg acaagcggcg aagtgcttct gaacgggaaa aatctggcgg gcgagcgttc gttcaaaatc tgccgcgcag gcattggttt cgtaccggaa gagcgtgaaa ccttcctcaa tctgaccgtg gatgaaaacc tgcaaatggg tattcagcgc ggtgcggaag gggcgaccag atggacggtc gaacagatgt tcgactattt cccgcgcctc aaggaacgcc gcaacacccg cgcgggcagc ttttcgggcg gcgagcagca gatgctgacc atgtgccgca cattgctcgg caatccgctg gtcatgctga tcgacgaacc gacagaaggc cttgcgccga agatcgtcgc tgtcgtcggc gagatgatca aggacatcaa ccggcagggc gtttcagtgg tgctggtgga gcagaagctc accattgcgc tgaaagtttc cagccgcgtc tgtgtgatgg ggcatggccg catcgttttt gaaggaacgc cggacgaact gaccgccgac gaaaaactga tggaggaatg gctggccgtc taagtgagta anataggatc cggctgnnnn nnnccccnnn annnnnnnnn nnnnnnnaan nngtnnnnnn ttnatnccnn nnnnnanccc nnnnangnnn gtnncncgnn ntacncncgc nnngtnnnnt gcgcncnnnc ntncnnnnnn nnncngcntn cnnnnnnnnn nnnnnnnnng nnnnnngnnn nnnannnnnn nnnnnaggna nnnnncnnnn gtttntnnnn nnnacnnnnn nnncnnnnnn nccnnnnnnn ncnncnncnn tcnnnnannn nnnnnnnccc cnnnnnnnnn nnnnnnnnnn nnnnannnnn nntnnnnnnn nnnnnnnnnn cnnnnnnnnn nnnnnnnnnn nnnnnnnnan nnnnnnnncn nnnnnncnnn nnnnnc

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggctg ctgaaaacat tctcgatgtt cgcgaattgc acgcgcatta cggcaagagc catatcctgc aaggcgtgac cttcgacctg aagaagggcg aggcgatcag cctgctgggg cgcaatggtt cgggccgttc caccaccatg aaggcgatca tggggctggt gccgccgaca agcggcgaag tgcttctgaa cgggaaaaat ctggcgggcg agcgttcgtt caaaatctgc cgcgcaggca ttggtttcgt accggaagag cgtgaaacct tcctcaatct gaccgtggat gaaaacctgc aaatgggtat tcagcgcggt gcggaagggg cgaccagatg gacggtcgaa cagatgttcg actatttccc gcgcctcaag gaacgccgca acacccgcgc gggcagcttt tcgggcggcg agcagcagat gctgaccatg tgccgcacat tgctcggcaa tccgctggtc atgctgatcg acgaaccgac agaaggcctt gcgccgaaga tcgtcgctgt cgtcggcgag atgatcaagg acatcaaccg gcagggcgtt tcagtggtgc tggtggagca gaagctcacc attgcgctga aagtttccag ccgcgtctgt gtgatggggc atggccgcat cgtttttgaa ggaacgccgg acgaactgac cgccgacgaa aaactgatgg aggaatggct ggccgtctga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at