BrsuA.10014.j

Ribose ABC transport system, ATP-binding protein RbsA (TC 3.A.1.2.1)

CENTER ID: BrsuA.10014.j
ORGANISM: Brucella suis 1330
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrsuA.10014.j.B1.GE36785 Full length( BrsuA.10014.j ) 1 492
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruSui107850_1661
BV-BRC: fig|204722.5.peg.1661
RefSeq: NP_698620.1
RefSeq: YP_005616443.1
UniProt: A0A0H3G4W1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEFLLDVEGL KKSFGGVAAL RDGRLQLRTG SVHALCGGNG AGKSTFLKIL MGIHARDGGH IRRRGKEVTY SSPAEALQAG IAIIEQELSP VPHMTVAENI YLGREPLRRF GGIDFKTMNL NAQKLLDQLE FKIRANQFMM NLSVAQVQLV EIAKALSHDA EVIFMDEPTS AIGEKEAQQL FAAIERLKAS GKGIVYVSHR LSEIFQIADS YTVFRDGSYI DSGDLGGITR AELIHMIVGR ELGEEYIKTN APTEIPGVEV SGLSAPGKIT DISFTAHKGE IFGIYGLMGS GRSEIFNCLF GLEKASQGTI RLEGEPVTVR RPADAVALGL AFVTEDRKLT GLNLGDSVRN NICLASLPEM SPHFSMNRRA ESAASREMIE RFRIKVAHDT MAVSGLSGGN QQKVVLGKWF LRKPRVLLLD EPTRGVDVGA KQEIYRIICE FAAQGGTVVM VSSEIDEVLG MSDRIMVMRG GQSVGIYRRA ETDAQSLVHL ST
NT Sequence
TTGGAATTTC TGCTCGATGT GGAGGGGCTC AAGAAGTCCT TTGGCGGTGT TGCTGCCTTG CGGGATGGGC GGCTGCAATT GCGTACCGGC TCGGTACATG CGCTGTGCGG CGGCAATGGC GCGGGCAAAT CCACGTTTTT GAAAATCCTG ATGGGCATTC ATGCCCGCGA TGGTGGACAT ATCCGCCGGC GTGGAAAAGA GGTTACGTAT TCCAGCCCCG CCGAAGCGTT GCAGGCGGGT ATCGCGATCA TCGAACAGGA GCTAAGCCCC GTTCCCCATA TGACCGTGGC GGAGAATATC TATTTGGGCA GGGAGCCGCT TCGCCGTTTC GGTGGCATCG ATTTCAAGAC GATGAACCTC AATGCCCAGA AGCTTTTGGA CCAGCTTGAG TTCAAGATTC GCGCCAACCA GTTCATGATG AACCTGTCTG TCGCGCAGGT GCAGCTTGTG GAAATAGCCA AGGCGCTCAG TCACGACGCT GAAGTCATTT TCATGGACGA ACCGACATCG GCCATTGGTG AAAAAGAAGC ACAGCAGCTT TTTGCAGCGA TAGAGCGTTT GAAAGCGAGC GGAAAAGGCA TTGTCTATGT CTCCCATCGC CTGTCGGAAA TTTTCCAGAT TGCCGATAGC TATACCGTGT TTCGCGACGG CAGTTATATC GATAGCGGCG ATCTCGGTGG CATCACCCGT GCGGAACTGA TCCATATGAT CGTCGGGCGG GAACTGGGTG AGGAATATAT CAAGACGAAT GCGCCTACGG AAATACCGGG CGTCGAGGTT TCCGGCCTCA GCGCGCCCGG CAAGATAACC GACATTTCCT TTACCGCGCA TAAGGGCGAG ATTTTTGGCA TTTACGGGTT GATGGGGTCA GGCCGCTCGG AAATATTCAA CTGTCTTTTC GGATTGGAGA AAGCCTCCCA AGGAACAATT CGGCTTGAAG GCGAACCCGT CACCGTCCGG CGCCCCGCGG ACGCCGTGGC TCTGGGGCTG GCTTTTGTGA CGGAAGATCG CAAGCTGACC GGCCTCAATC TCGGCGACAG CGTTCGCAAC AATATATGCC TTGCCAGCCT TCCCGAGATG AGTCCGCATT TTTCAATGAA TCGGCGTGCG GAATCGGCCG CCAGCAGGGA GATGATCGAG CGTTTCAGGA TCAAGGTTGC GCACGACACG ATGGCCGTTT CGGGCCTTTC CGGCGGCAAC CAGCAGAAGG TCGTGCTCGG AAAATGGTTC CTGCGAAAGC CACGCGTTCT GCTGCTCGAT GAACCCACTC GCGGTGTCGA TGTGGGTGCA AAACAGGAAA TTTATCGCAT CATTTGCGAA TTTGCCGCCC AAGGCGGGAC CGTCGTCATG GTTTCTTCCG AGATTGATGA AGTCCTCGGC ATGTCTGACC GCATCATGGT CATGCGTGGC GGACAATCGG TCGGCATCTA CCGCAGGGCG GAAACGGACG CGCAGTCATT GGTGCATCTT TCGACA
Details for BrsuA.10014.j.B1.GE36785
HARVESTED ON: 3/27/2013
SEQUENCED ON: 3/30/2013
EXPECTED MW: 55kDa
OBSERVED MW: 55kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 96
PERCENT COVERAGE: 59
Validated AA Sequence
MAHHHHHHML EFLLDVEGLK KSFGGVAALR DGRLQLRTGS VHALCGGNGA GKSTFLKILM GIHARDGGHI RRRGKEVTYS SPAEALQAGI AIIEQELSPV PHMTVAENIY LGREPLRRFG GIDFKTMNLN AQKLLDQLEF KIRANQFMMN LSVAQVQLVE IAKALSHDAE VIFMDEPTSA IGEKEAQQLF AAIERLKASG KGIVYVSHRL SEIFQIADSY TVFRDGSYID SGDLGGITRA ELIHMIVGRE LGEEYIKTNA PTEIPGVXVS GLSAPGKITD ISFTAHXXRF LAFXG
Validated NT Sequence
ttgnttactt taagaaggag anataccatg gctcaccacc accaccacca tatgttggaa tttctgctcg atgtggaggg gctcaagaag tcctttggcg gtgttgctgc cttgcgggat gggcggctgc aattgcgtac cggctcggta catgcgctgt gcggcggcaa tggcgcgggc aaatccacgt ttttgaaaat cctgatgggc attcatgccc gcgatggtgg acatatccgc cggcgtggaa aagaggttac gtattccagc cccgccgaag cgttgcaggc gggtatcgcg atcatcgaac aggagctaag ccccgttccc catatgaccg tggcggagaa tatctatttg ggcagggagc cgcttcgccg tttcggtggc atcgatttca agacgatgaa cctcaatgcc cagaagcttt tggaccagct tgagttcaag attcgcgcca accagttcat gatgaacctg tctgtcgcgc aggtgcagct tgtggaaata gccaaggcgc tcagtcacga cgctgaagtc attttcatgg acgaaccgac atcggccatt ggtgaaaaag aagcacagca gctttttgca gcgatagagc gtttgaaagc gagcggaaaa ggcattgtct atgtctccca tcgcctgtcg gaaattttcc agattgccga tagctatacc gtgtttcgcg acggcagtta tatcgatagc ggcgatctcg gtggcatcac ccgtgcggaa ctgatccata tgatcgtcgg gcgggaactg ggtgaggaat atatcaagac gaatgcgcct acggaaatac cgggcgtcna ggtttccggc ctcagcgcgc ccggcaagat aaccgacatt tcctttaccg cgcatannnn gagatttttg gcatttanng gttgatgggg tcnggccgct cggaaatnnt cnactgtctt ttcnnatnga naaagcctcc nnagnannnt nnnnnancnn tcnncnncnn nnncncnngn nncnngnnnn gggntgnttt nnnnangann ncnnnnctna nnnnnnnann nnnncnnnnn nnntnnnnnn nnnnnnnnnn nnnnnncnnn nntnannnna ncnnnnnnnc nnnnnnnnnn nnnnnnnnan nnnnnnngnn nnnnnnnnnn nnncnnnnnn nnggnnntnt
Expected Protein Sequence
MAHHHHHHME FLLDVEGLKK SFGGVAALRD GRLQLRTGSV HALCGGNGAG KSTFLKILMG IHARDGGHIR RRGKEVTYSS PAEALQAGIA IIEQELSPVP HMTVAENIYL GREPLRRFGG IDFKTMNLNA QKLLDQLEFK IRANQFMMNL SVAQVQLVEI AKALSHDAEV IFMDEPTSAI GEKEAQQLFA AIERLKASGK GIVYVSHRLS EIFQIADSYT VFRDGSYIDS GDLGGITRAE LIHMIVGREL GEEYIKTNAP TEIPGVEVSG LSAPGKITDI SFTAHKGEIF GIYGLMGSGR SEIFNCLFGL EKASQGTIRL EGEPVTVRRP ADAVALGLAF VTEDRKLTGL NLGDSVRNNI CLASLPEMSP HFSMNRRAES AASREMIERF RIKVAHDTMA VSGLSGGNQQ KVVLGKWFLR KPRVLLLDEP TRGVDVGAKQ EIYRIICEFA AQGGTVVMVS SEIDEVLGMS DRIMVMRGGQ SVGIYRRAET DAQSLVHLST
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttggaat ttctgctcga tgtggagggg ctcaagaagt cctttggcgg tgttgctgcc ttgcgggatg ggcggctgca attgcgtacc ggctcggtac atgcgctgtg cggcggcaat ggcgcgggca aatccacgtt tttgaaaatc ctgatgggca ttcatgcccg cgatggtgga catatccgcc ggcgtggaaa agaggttacg tattccagcc ccgccgaagc gttgcaggcg ggtatcgcga tcatcgaaca ggagctaagc cccgttcccc atatgaccgt ggcggagaat atctatttgg gcagggagcc gcttcgccgt ttcggtggca tcgatttcaa gacgatgaac ctcaatgccc agaagctttt ggaccagctt gagttcaaga ttcgcgccaa ccagttcatg atgaacctgt ctgtcgcgca ggtgcagctt gtggaaatag ccaaggcgct cagtcacgac gctgaagtca ttttcatgga cgaaccgaca tcggccattg gtgaaaaaga agcacagcag ctttttgcag cgatagagcg tttgaaagcg agcggaaaag gcattgtcta tgtctcccat cgcctgtcgg aaattttcca gattgccgat agctataccg tgtttcgcga cggcagttat atcgatagcg gcgatctcgg tggcatcacc cgtgcggaac tgatccatat gatcgtcggg cgggaactgg gtgaggaata tatcaagacg aatgcgccta cggaaatacc gggcgtcgag gtttccggcc tcagcgcgcc cggcaagata accgacattt cctttaccgc gcataagggc gagatttttg gcatttacgg gttgatgggg tcaggccgct cggaaatatt caactgtctt ttcggattgg agaaagcctc ccaaggaaca attcggcttg aaggcgaacc cgtcaccgtc cggcgccccg cggacgccgt ggctctgggg ctggcttttg tgacggaaga tcgcaagctg accggcctca atctcggcga cagcgttcgc aacaatatat gccttgccag ccttcccgag atgagtccgc atttttcaat gaatcggcgt gcggaatcgg ccgccagcag ggagatgatc gagcgtttca ggatcaaggt tgcgcacgac acgatggccg tttcgggcct ttccggcggc aaccagcaga aggtcgtgct cggaaaatgg ttcctgcgaa agccacgcgt tctgctgctc gatgaaccca ctcgcggtgt cgatgtgggt gcaaaacagg aaatttatcg catcatttgc gaatttgccg cccaaggcgg gaccgtcgtc atggtttctt ccgagattga tgaagtcctc ggcatgtctg accgcatcat ggtcatgcgt ggcggacaat cggtcggcat ctaccgcagg gcggaaacgg acgcgcagtc attggtgcat ctttcgacat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat