BrsuA.10014.i

Dipeptide transport ATP-binding protein DppD (TC 3.A.1.5.2)

CENTER ID: BrsuA.10014.i
ORGANISM: Brucella suis 1330
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrsuA.10014.i.B1.GE36784 Full length( BrsuA.10014.i ) 1 282
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruSui107850_1608
BV-BRC: fig|204722.5.peg.1608
RefSeq: NP_698574.1
RefSeq: YP_005616397.1
UniProt: A0A0H3G405

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLLDIKNLTV SFDTSTGPFK AVDGIDITVN KGEVLAIVGE SGSGKSVGML AVMGLLPKTA TVTADVMMFD GMDLRTLSDS QRRKIIGRDI SMIFQEPVAS LNPCFTVGYQ LEEVLKQHMG MGASASRARA IELLKLVGIR DAVERLKSFP HQMSGGQCQR VMIAIAIACN PKLLIADEPT TALDVTIQKQ ILDLLMRLQV EHGMGLIIIT HDMGVVAETA DRVIVQYKGH KMEDADVLSL FSAPKHPYTR ALLSALPENA TGNRLPTVSD FVFENISAGE AR
NT Sequence
GTGCTACTCG ATATTAAAAA TCTCACGGTT TCCTTCGATA CATCCACTGG CCCGTTCAAG GCGGTGGACG GGATCGACAT CACCGTTAAC AAGGGTGAAG TGCTGGCCAT CGTCGGCGAA TCCGGTTCGG GCAAGTCCGT CGGAATGCTG GCGGTTATGG GTCTTTTGCC AAAGACTGCG ACCGTAACCG CCGATGTGAT GATGTTCGAC GGCATGGACC TGCGCACCCT GTCCGACAGT CAGCGCCGCA AGATCATCGG CCGCGATATT TCGATGATCT TTCAGGAGCC CGTAGCAAGC CTCAATCCGT GTTTCACGGT CGGCTATCAG CTTGAGGAAG TCCTGAAGCA GCATATGGGT ATGGGGGCTT CTGCAAGCCG TGCCCGCGCT ATCGAGCTTC TGAAACTGGT CGGTATCCGT GATGCGGTGG AGCGTCTGAA AAGCTTCCCG CACCAGATGT CGGGCGGCCA GTGCCAGCGC GTGATGATCG CGATTGCGAT TGCCTGTAAT CCGAAGCTCC TGATTGCCGA CGAACCCACG ACCGCACTCG ATGTGACGAT CCAGAAGCAG ATTCTCGATC TCCTGATGCG GCTTCAGGTG GAGCACGGCA TGGGCCTCAT CATAATTACC CATGATATGG GTGTGGTGGC TGAAACGGCA GACCGGGTTA TCGTGCAATA CAAGGGCCAC AAGATGGAAG ATGCCGACGT GCTGTCGCTG TTTTCCGCGC CCAAGCACCC TTACACCCGT GCGCTGCTCT CGGCCCTGCC GGAAAATGCG ACTGGTAATC GTCTTCCGAC GGTTTCTGAT TTTGTCTTTG AGAATATCAG TGCAGGAGAA GCGCGA
Details for BrsuA.10014.i.B1.GE36784
HARVESTED ON: 3/27/2013
SEQUENCED ON: 3/30/2013
EXPECTED MW: 32kDa
OBSERVED MW: 32kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 97
Validated AA Sequence
MVLLDIKNLT VSFDTSTGPF KAVDGIDITV NKGEVLAIVG ESGSGKSVGM LAVMGLLPKT ATVTADVMMF DGMDLRTLSD SQRRKIIGRD ISMIFQEPVA SLNPCFTVGY QLEEVLKQHM GMGASASRAR AIELLKLVGI RDAVERLKSF PHQMSGGQCQ RVMIAIAIAC NPKLLIADEP TTALDVTIQK QILDLLMRLQ VEHGMGLIII THDMGVVAET ADRVIVQYKG HKMEDADVLS LFSAPKHPYT RALLSALPEN ATGNRLPTVS DFVFENISAG EAR
Validated NT Sequence
atcttactca cttatcgcgc ttctcctgca ctgatattct caaagacaaa atcagaaacc gtcggaagac gattaccagt cgcattttcc ggcagggccg agagcagcgc acgggtgtaa gggtgcttgg gcgcggaaaa cagcgacagc acgtcggcat cttccatctt gtggcccttg tattgcacga taacccggtc tgccgtttca gccaccacac ccatatcatg ggtaattatg atgaggccca tgccgtgctc cacctgaagc cgcatcagga gatcgagaat ctgcttctgg atcgtcacat cgagtgcggt cgtgggttcg tcggcaatca ggagcttcgg attacaggca atcgcaatcg cgatcatcac gcgctggcac tggccgcccg acatctggtg cgggaagctt ttcagacgct ccaccgcatc acggataccg accagtttca gaagctcgat agcgcgggca cggcttgcag aagcccccat acccatatgc tgcttcagga cttcctcaag ctgatagccg accgtgaaac acggattgag gcttgctacg ggctcctgaa agatcatcga aatatcgcgg ccgatgatct tgcggcgctg actgtcggac agggtgcgca ggtccatgcc gtcgaacatc atcacatcgg cggttacggt cgcagtcttt ggcaaaagac ccataaccgc cagcattccg acggacttgc ccgaaccgga ttcgccgacg atggccagca cttcaccctt gttaacggtg atgtcgatcc cgtccaccgc cttgaacggg ccagtggatg tatcgaagga aaccgtgaga tttttaatat cgagtagcac catatggtgg tgggtggtgg tgagccat
Expected Protein Sequence
MAHHHHHHML LDIKNLTVSF DTSTGPFKAV DGIDITVNKG EVLAIVGESG SGKSVGMLAV MGLLPKTATV TADVMMFDGM DLRTLSDSQR RKIIGRDISM IFQEPVASLN PCFTVGYQLE EVLKQHMGMG ASASRARAIE LLKLVGIRDA VERLKSFPHQ MSGGQCQRVM IAIAIACNPK LLIADEPTTA LDVTIQKQIL DLLMRLQVEH GMGLIIITHD MGVVAETADR VIVQYKGHKM EDADVLSLFS APKHPYTRAL LSALPENATG NRLPTVSDFV FENISAGEAR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgctac tcgatattaa aaatctcacg gtttccttcg atacatccac tggcccgttc aaggcggtgg acgggatcga catcaccgtt aacaagggtg aagtgctggc catcgtcggc gaatccggtt cgggcaagtc cgtcggaatg ctggcggtta tgggtctttt gccaaagact gcgaccgtaa ccgccgatgt gatgatgttc gacggcatgg acctgcgcac cctgtccgac agtcagcgcc gcaagatcat cggccgcgat atttcgatga tctttcagga gcccgtagca agcctcaatc cgtgtttcac ggtcggctat cagcttgagg aagtcctgaa gcagcatatg ggtatggggg cttctgcaag ccgtgcccgc gctatcgagc ttctgaaact ggtcggtatc cgtgatgcgg tggagcgtct gaaaagcttc ccgcaccaga tgtcgggcgg ccagtgccag cgcgtgatga tcgcgattgc gattgcctgt aatccgaagc tcctgattgc cgacgaaccc acgaccgcac tcgatgtgac gatccagaag cagattctcg atctcctgat gcggcttcag gtggagcacg gcatgggcct catcataatt acccatgata tgggtgtggt ggctgaaacg gcagaccggg ttatcgtgca atacaagggc cacaagatgg aagatgccga cgtgctgtcg ctgttttccg cgcccaagca cccttacacc cgtgcgctgc tctcggccct gccggaaaat gcgactggta atcgtcttcc gacggtttct gattttgtct ttgagaatat cagtgcagga gaagcgcgat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat