BrsuA.10014.g

L-proline glycine betaine ABC transport system permease protein ProV (TC 3.A.1.12.1)

CENTER ID: BrsuA.10014.g
ORGANISM: Brucella suis 1330
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrsuA.10014.g.B1.GE36782 Full length( BrsuA.10014.g ) 1 348
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruSui107850_1606
BV-BRC: fig|204722.5.peg.1606
RefSeq: NP_698572.1
RefSeq: YP_005616395.1
UniProt: A0A0H3G4S0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTIIALEDVC IIFGKNVDEA LHLADRGETR SRIQTETGLV LDVHNCTLDI EEGEILVLMG LSGSGKSTLL RAINRLNPIS RGRALVHDGE RSIDVGKADR ATLRRLRTEL VSMVFQQFGL LPWRSVEENV AFGLEISGMG KEERLDKARQ QLELVGLQDW ATRKVGELSG GMQQRVGLAR AFATGAPILL MDEPFSALDP LIRTRLQDEL LAFQSRLRKT IVFVSHDLDE AMKIGNRIAI MEGGRIVQCG TPQEILLHPA NEYVADFVAH MNPLGVLRAA DIMVPFDRNA APRPFAATAR RETLVRDLMT AVADSGGDVG IIENGAIVGK IAADDIVRAL AWHQQRGT
NT Sequence
ATGACGATTA TCGCACTTGA AGACGTTTGC ATCATCTTCG GCAAAAATGT CGATGAGGCG CTTCATCTGG CCGACCGCGG CGAAACACGC TCCAGGATCC AGACCGAAAC CGGCCTTGTG CTCGACGTCC ATAATTGTAC GCTCGATATC GAGGAAGGCG AAATCCTCGT CCTGATGGGC CTATCCGGCT CCGGAAAATC CACACTTCTG CGTGCAATCA ATCGTCTCAA CCCCATCTCG CGCGGGCGTG CACTGGTTCA CGATGGCGAG CGCAGCATCG ATGTGGGAAA AGCTGACCGC GCAACACTGC GCCGTCTGCG CACCGAGCTT GTCTCCATGG TGTTCCAACA GTTTGGTCTG CTGCCATGGC GTAGCGTCGA GGAAAATGTT GCCTTCGGGC TGGAAATCTC CGGTATGGGC AAGGAAGAAC GGCTGGACAA GGCGCGCCAG CAGCTTGAAC TCGTCGGCTT GCAGGATTGG GCCACCCGCA AGGTGGGTGA GCTTTCCGGC GGGATGCAGC AACGCGTCGG GCTTGCCCGC GCCTTTGCAA CCGGCGCGCC CATTCTCCTG ATGGACGAAC CGTTCTCCGC CCTCGACCCG CTGATTCGTA CCCGCCTGCA GGACGAATTG CTGGCCTTCC AGTCACGCCT GAGAAAAACC ATCGTTTTCG TCAGCCATGA CCTTGATGAA GCAATGAAGA TTGGCAATCG CATCGCCATC ATGGAAGGCG GGCGCATCGT TCAATGCGGG ACGCCGCAGG AGATTCTCCT GCACCCTGCC AATGAATATG TTGCCGACTT CGTTGCCCAT ATGAACCCGC TCGGCGTGCT TCGGGCCGCT GACATAATGG TGCCTTTCGA CCGCAATGCA GCGCCACGTC CTTTTGCTGC AACGGCCAGG CGCGAAACGC TGGTGCGGGA TTTGATGACT GCGGTGGCGG ATAGCGGTGG CGATGTCGGT ATCATTGAAA ACGGTGCCAT CGTCGGCAAG ATTGCAGCCG ATGATATCGT GCGCGCCCTC GCCTGGCATC AGCAGCGCGG AACA
Details for BrsuA.10014.g.B1.GE36782
HARVESTED ON: 3/27/2013
SEQUENCED ON: 3/30/2013
EXPECTED MW: 39kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 90
PERCENT COVERAGE: 108
Validated AA Sequence
MAHHHHHHMT IIALEDVCII FGKNVDEALH LADRGETRSR IQTETGLVLD VHNCTLDIEE GEILVLMGLS GSGKSTLLRA INRLNPISRG RALVHDGERS IDVGKADRAT LRRLRTELVS MVFQQFGLLP WRSVEENVAF GLEISGMGKE ERLDKARQQL ELVGLQDWAT RKVGELSGGM QQRVGLARAF ATGAPILLMD EPFSALDPLI RTRLQDELLA FQSRLRKTIV FVSHDLDEAM KIGNRIAIME GGRIVQCGTP XEILLHPANE YVADFVAHMN PLGVLRAADI MVPFDRNAAP RPFAATARRE TLVRDLMTAX XXSXGXXXXI EXXXHRXXXL XXXXXXXXXP XLXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXX
Validated NT Sequence
ttttgtnnnn tttaagaagg aganatacca tggctcacca ccaccaccac catatgacga ttatcgcact tgaagacgtt tgcatcatct tcggcaaaaa tgtcgatgag gcgcttcatc tggccgaccg cggcgaaaca cgctccagga tccagaccga aaccggcctt gtgctcgacg tccataattg tacgctcgat atcgaggaag gcgaaatcct cgtcctgatg ggcctatccg gctccggaaa atccacactt ctgcgtgcaa tcaatcgtct caaccccatc tcgcgcgggc gtgcactggt tcacgatggc gagcgcagca tcgatgtggg aaaagctgac cgcgcaacac tgcgccgtct gcgcaccgag cttgtctcca tggtgttcca acagtttggt ctgctgccat ggcgtagcgt cgaggaaaat gttgccttcg ggctggaaat ctccggtatg ggcaaggaag aacggctgga caaggcgcgc cagcagcttg aactcgtcgg cttgcaggat tgggccaccc gcaaggtggg tgagctttcc ggcgggatgc agcaacgcgt cgggcttgcc cgcgcctttg caaccggcgc gcccattctc ctgatggacg aaccgttctc cgccctcgac ccgctgattc gtacccgcct gcaggacgaa ttgctggcct tccagtcacg cctgagaaaa accatcgttt tcgtcagcca tgaccttgat gaagcaatga agattggcaa tcgcatcgcc atcatggaag gcgggcgcat cgttcaatgc gggacgccgc angagattct cctgcaccct gccaatgaat atgttgccga cttcgttgcc catatgaacc cgctcggcgt gcttcgggcc gctgacataa tggtgccttt cgaccgcaat gcagcgccac gtccttttgc tgcaacggcc aggcgcgaaa cgctggtgcg ggatttgatg actgcgnnnn ngganagcgn tggcgnnnnn nnnntcattg aaaannggng ccatcgncnn gnannattgn nnncnnannn nnnnnnnngn gnnnncccnn nnctggnann ngnnnnnnnn nnnnnnannn nnannnnnnn nnnnnnnnnn nnnnnnnnan nnannnaann nntnnnnnnn cnnnnntnnn nnnnnncnnn nnnnnncc
Expected Protein Sequence
MAHHHHHHMT IIALEDVCII FGKNVDEALH LADRGETRSR IQTETGLVLD VHNCTLDIEE GEILVLMGLS GSGKSTLLRA INRLNPISRG RALVHDGERS IDVGKADRAT LRRLRTELVS MVFQQFGLLP WRSVEENVAF GLEISGMGKE ERLDKARQQL ELVGLQDWAT RKVGELSGGM QQRVGLARAF ATGAPILLMD EPFSALDPLI RTRLQDELLA FQSRLRKTIV FVSHDLDEAM KIGNRIAIME GGRIVQCGTP QEILLHPANE YVADFVAHMN PLGVLRAADI MVPFDRNAAP RPFAATARRE TLVRDLMTAV ADSGGDVGII ENGAIVGKIA ADDIVRALAW HQQRGT
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacga ttatcgcact tgaagacgtt tgcatcatct tcggcaaaaa tgtcgatgag gcgcttcatc tggccgaccg cggcgaaaca cgctccagga tccagaccga aaccggcctt gtgctcgacg tccataattg tacgctcgat atcgaggaag gcgaaatcct cgtcctgatg ggcctatccg gctccggaaa atccacactt ctgcgtgcaa tcaatcgtct caaccccatc tcgcgcgggc gtgcactggt tcacgatggc gagcgcagca tcgatgtggg aaaagctgac cgcgcaacac tgcgccgtct gcgcaccgag cttgtctcca tggtgttcca acagtttggt ctgctgccat ggcgtagcgt cgaggaaaat gttgccttcg ggctggaaat ctccggtatg ggcaaggaag aacggctgga caaggcgcgc cagcagcttg aactcgtcgg cttgcaggat tgggccaccc gcaaggtggg tgagctttcc ggcgggatgc agcaacgcgt cgggcttgcc cgcgcctttg caaccggcgc gcccattctc ctgatggacg aaccgttctc cgccctcgac ccgctgattc gtacccgcct gcaggacgaa ttgctggcct tccagtcacg cctgagaaaa accatcgttt tcgtcagcca tgaccttgat gaagcaatga agattggcaa tcgcatcgcc atcatggaag gcgggcgcat cgttcaatgc gggacgccgc aggagattct cctgcaccct gccaatgaat atgttgccga cttcgttgcc catatgaacc cgctcggcgt gcttcgggcc gctgacataa tggtgccttt cgaccgcaat gcagcgccac gtccttttgc tgcaacggcc aggcgcgaaa cgctggtgcg ggatttgatg actgcggtgg cggatagcgg tggcgatgtc ggtatcattg aaaacggtgc catcgtcggc aagattgcag ccgatgatat cgtgcgcgcc ctcgcctggc atcagcagcg cggaacatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at