SSGCID: Target Page

BrsuA.10014.c

ABC-type polar amino acid transport system, ATPase component

CENTER ID:	BrsuA.10014.c
ORGANISM:	Brucella suis 1330
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrsuA.10014.c.B1.GE36778	Full length( BrsuA.10014.c )		1	269

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	PATRIC_Locus_Tag:VBIBruSui107850_0759
BV-BRC:	fig\|204722.5.peg.759
RefSeq:	NP_697759.1
RefSeq:	YP_005615583.1
UniProt:	A0A0H3G664

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSAQSALQQS GIGIEVDRSK MQVSKTEVAI EITNMHKWYG EFHVLRDINL KVMRGERIVV AGPSGSGKST MIRCINRLEE HQKGKIVVDG IELTNDLKKI DEVRREVGMV FQHFNLFPHL TILENCTLAP IWVRKMPKKQ AEEIAMHYLE RVKIPEQANK YPGQLSGGQQ QRVAIARALC MNPKVMLFDE PTSALDPEMV KEVLDTMVSL AAEGMTMICV THEMGFARQV ANRVIFMDQG QIVEQNSPDE FFDNPLHERT RLFLSQILH

NT Sequence

ATGAGTGCAC AAAGCGCCCT CCAGCAGTCC GGGATCGGGA TCGAAGTCGA CCGCTCGAAA ATGCAGGTTT CCAAAACCGA AGTCGCCATC GAGATCACCA ATATGCACAA GTGGTATGGT GAGTTCCACG TTCTGCGTGA CATCAACCTC AAGGTTATGC GTGGTGAGCG TATCGTCGTG GCCGGACCGT CCGGTTCTGG CAAATCGACC ATGATCCGCT GCATCAACCG TCTGGAAGAA CATCAGAAAG GCAAGATCGT CGTCGATGGT ATCGAGCTTA CCAACGATCT GAAGAAAATC GATGAAGTGC GCCGCGAAGT CGGCATGGTG TTCCAGCACT TCAACCTCTT CCCGCATCTA ACCATTCTGG AAAACTGCAC GCTTGCGCCG ATCTGGGTGC GCAAGATGCC GAAAAAGCAG GCGGAAGAAA TCGCCATGCA TTATCTGGAG CGTGTGAAGA TCCCGGAACA GGCCAACAAA TATCCGGGCC AGCTTTCCGG CGGCCAGCAG CAGCGTGTGG CGATTGCGCG CGCGCTTTGC ATGAACCCGA AAGTCATGCT GTTCGATGAG CCGACCTCGG CGCTCGATCC GGAAATGGTC AAGGAAGTGC TGGATACGAT GGTGAGCCTT GCTGCCGAAG GCATGACGAT GATCTGCGTG ACGCACGAAA TGGGCTTTGC CCGTCAGGTT GCCAACCGCG TCATCTTCAT GGATCAGGGC CAGATCGTCG AACAGAATTC GCCAGACGAA TTCTTCGATA ATCCGCTGCA TGAACGCACC AGGCTGTTCC TGAGCCAGAT CCTGCAT

Details for BrsuA.10014.c.B1.GE36778

HARVESTED ON:	3/27/2013
SEQUENCED ON:	3/30/2013
EXPECTED MW:	32kDa
OBSERVED MW:	32kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	98
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMS AQSALQQSGI GIEVDRSKMQ VSKTEVAIEI TNMHKWYGEF HVLRDINLKV MRGERIVVAG PSGSGKSTMI RCINRLEEHQ KGKIVVDGIE LTNDLKKIDE VRREVGMVFQ HFNLFPHLTI LENCTLAPIW VRKMPKKQAE EIAMHYLERV KIPEQANKYP GQLSGGQQQR VAIARALCMN PKVMLFDEPT SALDPEMVKE VLDTMVSLAA EGMTMICVTH EMGFARQVAN RVIFMDXGQI VEQNSPDEFF DNPLHERTXC S

Validated NT Sequence

attttgttta ctttaagaag gaganatacc atggctcacc accaccacca ccatatgagt gcacaaagcg ccctccagca gtccgggatc gggatcgaag tcgaccgctc gaaaatgcag gtttccaaaa ccgaagtcgc catcgagatc accaatatgc acaagtggta tggtgagttc cacgttctgc gtgacatcaa cctcaaggtt atgcgtggtg agcgtatcgt cgtggccgga ccgtccggtt ctggcaaatc gaccatgatc cgctgcatca accgtctgga agaacatcag aaaggcaaga tcgtcgtcga tggtatcgag cttaccaacg atctgaagaa aatcgatgaa gtgcgccgcg aagtcggcat ggtgttccag cacttcaacc tcttcccgca tctaaccatt ctggaaaact gcacgcttgc gccgatctgg gtgcgcaaga tgccgaaaaa gcaggcggaa gaaatcgcca tgcattatct ggagcgtgtg aagatcccgg aacaggccaa caaatatccg ggccagcttt ccggcggcca gcagcagcgt gtggcgattg cgcgcgcgct ttgcatgaac ccgaaagtca tgctgttcga tgagccgacc tcggcgctcg atccggaaat ggtcaaggaa gtgctggata cgatggtgag ccttgctgcc gaaggcatga cgatgatctg cgtgacgcac gaaatgggct ttgcccgtca ggttgccaac cgcgtcatct tcatggatca nggccagatc gtcgaacaga attcgccaga cgaattcttc gataatccgc tgcatgaacg caccnnntgt tcctgagcca gatcctgcat taagtgagta aganagnatc cggctnnnnn nnnnnccccn nnnanannnn nnnnnnannn nnnnaannnn cccgnnttnn tcnnnnnnnn nntccccccc nngnnncnnn nnnnncnctt cnccnnnnnn ccntnnnnnc nnccnnncnc nncctnccnn nnnnnnnnnn nntnctcnnn nnnnnnnnnn ccnnnnnnnn cnacnnnncc tnccccnncc tncgctnnnn nnccctnnnn nnnccctttn nnnnntttnn nnnnnnnnnc cnnnnnnnc

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagtg cacaaagcgc cctccagcag tccgggatcg ggatcgaagt cgaccgctcg aaaatgcagg tttccaaaac cgaagtcgcc atcgagatca ccaatatgca caagtggtat ggtgagttcc acgttctgcg tgacatcaac ctcaaggtta tgcgtggtga gcgtatcgtc gtggccggac cgtccggttc tggcaaatcg accatgatcc gctgcatcaa ccgtctggaa gaacatcaga aaggcaagat cgtcgtcgat ggtatcgagc ttaccaacga tctgaagaaa atcgatgaag tgcgccgcga agtcggcatg gtgttccagc acttcaacct cttcccgcat ctaaccattc tggaaaactg cacgcttgcg ccgatctggg tgcgcaagat gccgaaaaag caggcggaag aaatcgccat gcattatctg gagcgtgtga agatcccgga acaggccaac aaatatccgg gccagctttc cggcggccag cagcagcgtg tggcgattgc gcgcgcgctt tgcatgaacc cgaaagtcat gctgttcgat gagccgacct cggcgctcga tccggaaatg gtcaaggaag tgctggatac gatggtgagc cttgctgccg aaggcatgac gatgatctgc gtgacgcacg aaatgggctt tgcccgtcag gttgccaacc gcgtcatctt catggatcag ggccagatcg tcgaacagaa ttcgccagac gaattcttcg ataatccgct gcatgaacgc accaggctgt tcctgagcca gatcctgcat tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat