SSGCID: Target Page

BrsuA.10014.b

Zinc import ATP-binding protein ZnuC (EC 3.6.3.-)

CENTER ID:	BrsuA.10014.b
ORGANISM:	Brucella suis 1330
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrsuA.10014.b.A1.GE34020	full length		1	298

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|204722.5.peg.3351
RefSeq:	NP_700278.1
UniProt:	Q8FUU5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDKKSSHPAG AARDILIELR NAGVYRDGRW LVRNVDLSVE RGEIVTLIGP NGAGKSTAAK MALHILKPDE GMVSHKPGLR IGYVPQKINI DRTLPLSVER LMTLTGPLPR KEIDAALEAV GIAHLAKAET AHLSGGEFQR ALMARALARK PDIMVLDEPV QGVDFSGEAA LYELIARLRD DTGCGVLLSS HDLHLVMAAT DRVICLNGHV CCSGTPRDVT SSPEYVRLFG SRAVGPLAVY EHHHDHTHLP DGRVLYADGT TADPIAGSTM GPRGHCHVED GHHHDHEHHH HEGGQPRA

NT Sequence

atggataaga aatcctctca ccccgccggg gcggcgcgag acatattgat tgaactgagg aatgccgggg tttatcgcga tggccgctgg ctggtgcgca atgtcgatct gagcgtggag cgcggcgaga tcgtgaccct catcggcccg aacggtgccg gcaaaagcac cgccgccaag atggcgctgc atattctcaa gccggacgaa ggcatggtgt cccacaagcc gggcctgcgt attggttatg tgccgcagaa gatcaatatc gaccgcacct tgccgctttc ggtcgaacgc ctgatgacgc tgacggggcc gcttccccgc aaggaaattg atgcggcact tgaagccgtc ggcattgccc atcttgcgaa agcggaaacg gcgcatcttt ccggcggcga gttccagcgc gcgctgatgg cgcgcgcgct cgcccgcaag cctgacatca tggtgctgga cgagccggtg cagggcgtcg atttttccgg cgaggcggca ctttacgaac tgattgccag gcttcgcgac gatacgggtt gcggtgtgct actgagttcg cacgatctgc atctggttat ggccgcgacc gaccgcgtca tctgcctgaa cggccatgtc tgttgcagcg gcaccccgcg cgacgtgaca tccagccccg aatatgtccg cctgttcggc agccgcgctg tcggcccgct cgcagtctat gaacatcacc acgatcatac acatctgccc gatggccgtg tgctttatgc cgatggaacc acagcggatc ccatagcagg atccacaatg ggaccaaggg gacattgcca cgttgaagac ggtcatcacc atgaccatga acaccatcat cacgaagggg gccagccgcg tgcttga

Details for BrsuA.10014.b.A1.GE34020

HARVESTED ON:	6/4/2012
SEQUENCED ON:	6/5/2012
EXPECTED MW:	34kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMDKKSSHPA GAARDILIEL RNAGVYRDGR WLVRNVDLSV ERGEIVTLIG PNGAGKSTAA KMALHILKPD EGMVSHKPGL RIGYVPQKIN IDRTLPLSVE RLMTLTGPLP RKEIDAALEA VGIAHLAKAE TAHLSGGEFQ RALMARALAR KPDIMVLDEP VQGVDFSGEA ALYELIARLR DDTGCGVLLS SHDLHLVMAA TDRVICLNGH VCCSGTPRDV TSSPEYVRLF GSRAVGPLAV YEHHHDHTHL PDGRVLYADG TTADPIAGST MGPRGHCHVE DGHHHDHEHH HHEGGQPRA

Validated NT Sequence

ttattaagca cgcggctggc ccccttcgtg atgatggtgt tcatggtcat ggtgatgacc gtcttcaacg tggcaatgtc cccttggtcc cattgtggat cctgctatgg gatccgctgt ggttccatcg gcataaagca cacggccatc gggcagatgt gtatgatcgt ggtgatgttc atagactgcg agcgggccga cagcgcggct gccgaacagg cggacatatt cggggctgga tgtcacgtcg cgcggggtgc cgctgcaaca gacatggccg ttcaggcaga tgacgcggtc ggtcgcggcc ataaccagat gcagatcgtg cgaactcagt agcacaccgc aacccgtatc gtcgcgaagc ctggcaatca gttcgtaaag tgccgcctcg ccggaaaaat cgacgccctg caccggctcg tccagcacca tgatgtcagg cttgcgggcg agcgcgcgcg ccatcagcgc gcgctggaac tcgccgccgg aaagatgcgc cgtttccgct ttcgcaagat gggcaatgcc gacggcttca agtgccgcat caatttcctt gcggggaagc ggccccgtca gcgtcatcag gcgttcgacc gaaagcggca aggtgcggtc gatattgatc ttctgcggca cataaccaat acgcaggccc ggcttgtggg acaccatgcc ttcgtccggc ttgagaatat gcagcgccat cttggcggcg gtgcttttgc cggcaccgtt cgggccgatg agggtcacga tctcgccgcg ctccacgctc agatcgacat tgcgcaccag ccagcggcca tcgcgataaa ccccggcatt cctcagttca atcaatatgt ctcgcgccgc cccggcgggg tgagaggatt tcttatccat cgaaccagga ccctgggtct gagcttccag ggtacccata tgatggtgat ggtgatgagc cat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggataagaaa tcctctcacc ccgccggggc ggcgcgagac atattgattg aactgaggaa tgccggggtt tatcgcgatg gccgctggct ggtgcgcaat gtcgatctga gcgtggagcg cggcgagatc gtgaccctca tcggcccgaa cggtgccggc aaaagcaccg ccgccaagat ggcgctgcat attctcaagc cggacgaagg catggtgtcc cacaagccgg gcctgcgtat tggttatgtg ccgcagaaga tcaatatcga ccgcaccttg ccgctttcgg tcgaacgcct gatgacgctg acggggccgc ttccccgcaa ggaaattgat gcggcacttg aagccgtcgg cattgcccat cttgcgaaag cggaaacggc gcatctttcc ggcggcgagt tccagcgcgc gctgatggcg cgcgcgctcg cccgcaagcc tgacatcatg gtgctggacg agccggtgca gggcgtcgat ttttccggcg aggcggcact ttacgaactg attgccaggc ttcgcgacga tacgggttgc ggtgtgctac tgagttcgca cgatctgcat ctggttatgg ccgcgaccga ccgcgtcatc tgcctgaacg gccatgtctg ttgcagcggc accccgcgcg acgtgacatc cagccccgaa tatgtccgcc tgttcggcag ccgcgctgtc ggcccgctcg cagtctatga acatcaccac gatcatacac atctgcccga tggccgtgtg ctttatgccg atggaaccac agcggatccc atagcaggat ccacaatggg accaagggga cattgccacg ttgaagacgg tcatcaccat gaccatgaac accatcatca cgaagggggc cagccgcgtg cttaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa