SSGCID: Target Page

BrsuA.01664.a

Sensor histidine kinase BvrS, putative

CENTER ID:	BrsuA.01664.a
ORGANISM:	Brucella suis 1330
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrsuA.01664.a.A2.GE34015	domain1		69	286

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|204722.5.peg.2128
RefSeq:	NP_699066.1
UniProt:	A0A0H3G5U9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MVAETQKDSL SGMRERRARR QRSVFLRRFL SPLRKFLGQY LFSSLTRRIL FLNLAALAVL VSGILYMNQF REGLIDAKIE SLLTQGKIIA AAISASATVD TNSLLIDPEK LLELQAGQSI TPSPDSPDNW EFPINPEKVS PLLRQLISPT STRARIYDRY ANKLLDSRAL YSTSFPSSGP VLRYDLPPIE DETPALWERI GSWLSRLFYG GGLPLYQEQP GGNGLAYQEI VKALSGSPQM AQRRNQRGEL IVSVAVPIQR SRAILGVLLL STEGDDIDKI VQAERMAVFR VFGVVSAVMV ILSLFLASTI ANPLRKLSAA ADRVRHGVKN RVEIPDFSER QDEVGHLSTS IRDMTDALYT RIEAIESFAA DVSHELKNPL TSLRSAVETL PLAKTDESRK RLLDVIQHDV RRLDRLITDI SDVSRLDAEL AREHIDRVDM KKLLTSLVTA AREVRRNKVG TEIVFNTGKL PTGKKGFYVA GHDLRFGQVV SNLIENARSF VPDDTGRIVV TLAGEGNRLR ILVEDNGPGI PIENIERIFE RFYTDRPASE AFGQNSGLGL SISRQIIEAH GGTLTAENIT DPDKPDIFKG ARFIVDLPAS A

NT Sequence

atggtcgcag agacccagaa agatagcctt tcggggatgc gcgagcgcag agcacggcgg caacgctcgg tgtttctgcg ccgctttctg tcgcccttgc gcaagtttct tgggcaatat ctcttttcaa gcctgacgcg ccgcatcctg tttctcaacc tggcagcact ggccgttctg gtttccggca ttctttatat gaaccagttc cgcgaggggc tgatcgacgc caagatcgaa agcctgctga cgcagggcaa gatcatcgct gctgcgattt ctgcatcggc tacggtcgat accaattcgc tgctgatcga ccctgaaaag cttctggagc ttcaggccgg gcagagcata accccctccc ccgattcgcc ggataattgg gaatttccga tcaatcccga aaaggtttcg ccgctgctgc gccagttgat ctctccgacc agcacgcgcg cgcgcatcta tgaccgctat gcgaataagc tgcttgattc gcgcgcgctt tattcgacaa gcttcccgtc cagcgggccg gtcctgcgct atgacctgcc gccgatagag gatgagacgc ctgccctttg ggaacgtatc ggcagctggc tgtcgcgcct tttctatggc ggtggcctgc cgctctacca ggagcagccg ggcggaaacg gccttgccta tcaggaaatc gtcaaggcgc tgagcggctc gccgcagatg gcacagcgcc gcaatcagcg cggtgagctg atcgtttccg tggccgttcc tatccagcgc tcccgtgcca ttctgggtgt tctgcttctt tctaccgaag gcgatgatat cgacaagatc gttcaggccg agcgtatggc ggttttccgc gtgttcggcg ttgtgtcggc ggtcatggtc atcctgtcgc ttttcctcgc ctcaacaatt gccaacccct tgcgcaagct ttcggctgcg gcggaccggg tgcgccatgg cgtgaaaaac cgcgtggaaa ttcccgattt ttccgagcgt caggacgagg tgggccacct ttccacctcc atccgcgaca tgacggacgc gctttatacg cgcatcgaag ccattgaaag cttcgcagcc gatgtcagcc atgagctgaa gaaccccctc acctcgcttc gcagcgcagt cgaaaccctg ccgctcgcca agaccgacga atcacgcaag cgcctgctcg atgtcatcca gcacgacgtg cggcggctgg accgccttat cacggatatt tccgacgttt cgcgcctcga tgccgagctt gcgcgcgaac atatcgaccg cgtggatatg aaaaagcttc tcaccagcct tgtgactgcc gcccgcgaag tgcggcgcaa caaggtcggc accgaaatcg tgttcaacac gggcaagctg cctaccggca agaaaggctt ctatgtcgcc ggccacgatc tgcgttttgg gcaggtggta agcaacctta tcgagaatgc gcgctccttc gtgcctgacg atacgggccg tatcgtcgtt acgctggccg gcgaaggcaa ccggctgcgc attctggtcg aggataacgg ccccggcatt cctatcgaga atatcgagcg catcttcgag cgtttctaca cggatcgccc ggcatcggaa gcattcgggc agaattccgg cctcggcctg tcgatcagcc ggcagatcat cgaggcgcat ggcggcacgc tgacggcgga aaatattacc gatccggata agccggatat tttcaagggt gcccgcttta tcgttgatct tccggccagc gcatga

Details for BrsuA.01664.a.A2.GE34015

HARVESTED ON:	11/21/2011
SEQUENCED ON:	11/29/2011
EXPECTED MW:	26kDa
OBSERVED MW:	26kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMQFREGLID AKIESLLTQG KIIAAAISAS ATVDTNSLLI DPEKLLELQA GQSITPSPDS PDNWEFPINP EKVSPLLRQL ISPTSTRARI YDRYANKLLD SRALYSTSFP SSGPVLRYDL PPIEDETPAL WERIGSWLSR LFYGGGLPLY QEQPGGNGLA YQEIVKALSG SPQMAQRRNQ RGELIVSVAV PIQRSRAILG VLLLSTEGDD IDXIVQAERM

Validated NT Sequence

ttnnttantt taagaaggag anataccatg gctcatcacc atcaccatca tatgggtacc ctggaagctc agacccaggg tcctggttcg atgcagttcc gcgaggggct gatcgacgcc aagatcgaaa gcctgctgac gcagggcaag atcatcgctg ctgcgatttc tgcatcggct acggtcgata ccaattcgct gctgatcgac cctgaaaagc ttctggagct tcaggccggg cagagcataa ccccctcccc cgattcgccg gataattggg aatttccgat caatcccgaa aaggtttcgc cgctgctgcg ccagttgatc tctccgacca gcacgcgcgc gcgcatctat gaccgctatg cgaataagct gcttgattcg cgcgcgcttt attcgacaag cttcccgtcc agcgggccgg tcctgcgcta tgacctgccg ccgatagagg atgagacgcc tgccctttgg gaacgtatcg gcagctggct gtcgcgcctt ttctatggcg gtggcctgcc gctctaccag gagcagccgg gcggaaacgg ccttgcctat caggaaatcg tcaaggcgct gagcggctcg ccgcagatgg cacagcgccg caatcagcgc ggtgagctga tcgtttccgt ggccgttcct atccagcgct cccgtgccat tctgggtgtt ctgcttcttt ctaccgaagg cgatgatatc gacnagatcg ttcaggccga gcgtatgtaa taaacagcac gaacaagttc tgcagccaag cttctcgagg anccggctgc taacaaagcc cgaaaggaag ctganttggc tgnngnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngnnngna tnnnnngtnn nnnnntnnnn nnncnnnccn ctngncnntt cnnngngnng nnnngnnnnn nnnnnannnt nnnnnnnngn aananntnnn nngcnnnnnn cangannnnn nnnnngnnnc nnnnangnna ntnnntng

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcagttccgc gaggggctga tcgacgccaa gatcgaaagc ctgctgacgc agggcaagat catcgctgct gcgatttctg catcggctac ggtcgatacc aattcgctgc tgatcgaccc tgaaaagctt ctggagcttc aggccgggca gagcataacc ccctcccccg attcgccgga taattgggaa tttccgatca atcccgaaaa ggtttcgccg ctgctgcgcc agttgatctc tccgaccagc acgcgcgcgc gcatctatga ccgctatgcg aataagctgc ttgattcgcg cgcgctttat tcgacaagct tcccgtccag cgggccggtc ctgcgctatg acctgccgcc gatagaggat gagacgcctg ccctttggga acgtatcggc agctggctgt cgcgcctttt ctatggcggt ggcctgccgc tctaccagga gcagccgggc ggaaacggcc ttgcctatca ggaaatcgtc aaggcgctga gcggctcgcc gcagatggca cagcgccgca atcagcgcgg tgagctgatc gtttccgtgg ccgttcctat ccagcgctcc cgtgccattc tgggtgttct gcttctttct accgaaggcg atgatatcga caagatcgtt caggccgagc gtatgtaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa