BrsuA.01563.a
Dihydrodipicolinate synthase (DHDPS) (EC 4.2.1.52)
| CENTER ID: | BrsuA.01563.a |
| ORGANISM: | Brucella suis 1330 |
| ASSOCIATED DISEASE: | Brucellosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrsuA.01563.a.A1.GE34017 | full length | 1 | 293 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrsuA.01563.a.A1.PW34594 | full length | 1 | 293 |
Structures
6XGS
DEPOSITED: 6/18/2020
DETERMINATION: XRay
CLONE: BrsuA.01563.a.A1.GE34017
PROTEIN: BrsuA.01563.a.A1.PW34594
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_127458 |
| PATRIC ID: | fig|204722.5.peg.660 |
| RefSeq: | NP_697660.1 |
| UniProt: | Q8G1R0 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLKGSITALV TPFDREGAFD EKAFRAFVNW QIEEGTKGLV PVGTTGETPT LSHDEHKRVI EVCIEVAAGR VPVIAGAGSN NTVEAIELAQ HAEKAGADAV LVVTPYYNKP NQRGLYEHFS RVVRSISIPL VIYNIPGRSI IDMTPETMGA LVRDCKNIVG VKDATGKIER VSEQRAICGK EFIQLSGEDA TALGFNAHGG VGCISVTSNI APRLCAEFQE ACQAGNFAKA LELQDRLMPL HKALFLEPNP SGPKYALSRL GRIENVLRSP MVTIEAATAE KIDHAMKHAG LIN
NT Sequence
atgctgaagg gctcaattac cgcacttgtc acgccgttcg accgtgaagg ggcgttcgac gaaaaagcct ttcgtgcatt cgtcaactgg cagatcgaag aggggacgaa aggtctcgtt ccggttggca cgaccggcga aacgccgacc ctttcccatg atgaacataa gcgtgtgatc gaagtctgca ttgaagtcgc cgctggccgt gtgccagtga ttgcgggtgc cggctcgaac aatacggtgg aagcgatcga actggcgcag cacgccgaaa aagcgggcgc ggatgcggtt ctggttgtca caccttatta caacaagccg aatcagcgtg gcctctacga gcatttttcg cgcgttgtcc gctctatctc gatcccgctg gtgatctata acattccggg ccggtccatt atcgacatga cgccggaaac catgggcgct ctcgttcgcg actgtaagaa tattgtcggc gtcaaggatg cgaccggcaa gatcgagcgt gtttccgaac agcgcgcgat ctgtggcaag gagttcatcc agctttccgg tgaagacgcc acggcgcttg gcttcaatgc tcatggcggg gtggggtgca tttcggtaac gtccaacatt gcgccgcgtc tttgcgcgga atttcaggaa gcctgccagg ctggtaattt cgccaaggcg ctggaattgc aggaccgttt gatgccgctg cacaaggcgc tgttcctgga acccaatcct tccggcccga aatatgcgct ttcgcgcctg ggtcgtattg aaaatgttct gcgttcacca atggttacga tcgaagcggc gacggcggaa aagatcgacc atgccatgaa gcacgcgggc ctcatcaact ga
Details for BrsuA.01563.a.A1.GE34017
| HARVESTED ON: | 11/21/2011 |
| SEQUENCED ON: | 11/29/2011 |
| EXPECTED MW: | 33kDa |
| OBSERVED MW: | 33kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLKGSITAL VTPFDREGAF DEKAFRAFVN WQIEEGTKGL VPVGTTGETP TLSHDEHKRV IEVCIEVAAG RVPVIAGAGS NNTVEAIELA QHAEKAGADA VLVVTPYYNK PNQRGLYEHF SRVVRSISIP LVIYNIPGRS IIDMTPETMG ALVRDCKNIV GVKDATGKIE RVSEQRAICG KEFIQLSGED ATALGFNAHG GVGCISVTSN IAPRLCAEFQ EACQAGNFAK ALELQDRLMP LHKALFLEPN PSGPKYALSR LGRIENVLRS PMVTIEAATA EKIDHAMKHA GLIN
Validated NT Sequence
ttattagttg atgaggcccg cgtgcttcat ggcatggtcg atcttttccg ccgtcgccgc ttcgatcgta accattggtg aacgcagaac attttcaata cgacccaggc gcgaaagcgc atatttcggg ccggaaggat tgggttccag gaacagcgcc ttgtgcagcg gcatcaaacg gtcctgcaat tccagcgcct tggcgaaatt accagcctgg caggcttcct gaaattccgc gcaaagacgc ggcgcaatgt tggacgttac cgaaatgcac cccaccccgc catgagcatt gaagccaagc gccgtggcgt cttcaccgga aagctggatg aactccttgc cacagatcgc gcgctgttcg gaaacacgct cgatcttgcc ggtcgcatcc ttgacgccga caatattctt acagtcgcga acgagagcgc ccatggtttc cggcgtcatg tcgataatgg accggcccgg aatgttatag atcaccagcg ggatcgagat agagcggaca acgcgcgaaa aatgctcgta gaggccacgc tgattcggct tgttgtaata aggtgtgaca accagaaccg catccgcgcc cgctttttcg gcgtgctgcg ccagttcgat cgcttccacc gtattgttcg agccggcacc cgcaatcact ggcacacggc cagcggcgac ttcaatgcag acttcgatca cacgcttatg ttcatcatgg gaaagggtcg gcgtttcgcc ggtcgtgcca accggaacga gacctttcgt cccctcttcg atctgccagt tgacgaatgc acgaaaggct ttttcgtcga acgccccttc acggtcgaac ggcgtgacaa gtgcggtaat tgagcccttc agcatcgaac caggaccctg ggtctgagct tccagggtac ccatatgatg gtgatggtga tgagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLKGSITAL VTPFDREGAF DEKAFRAFVN WQIEEGTKGL VPVGTTGETP TLSHDEHKRV IEVCIEVAAG RVPVIAGAGS NNTVEAIELA QHAEKAGADA VLVVTPYYNK PNQRGLYEHF SRVVRSISIP LVIYNIPGRS IIDMTPETMG ALVRDCKNIV GVKDATGKIE RVSEQRAICG KEFIQLSGED ATALGFNAHG GVGCISVTSN IAPRLCAEFQ EACQAGNFAK ALELQDRLMP LHKALFLEPN PSGPKYALSR LGRIENVLRS PMVTIEAATA EKIDHAMKHA GLIN
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gctgaagggc tcaattaccg cacttgtcac gccgttcgac cgtgaagggg cgttcgacga aaaagccttt cgtgcattcg tcaactggca gatcgaagag gggacgaaag gtctcgttcc ggttggcacg accggcgaaa cgccgaccct ttcccatgat gaacataagc gtgtgatcga agtctgcatt gaagtcgccg ctggccgtgt gccagtgatt gcgggtgccg gctcgaacaa tacggtggaa gcgatcgaac tggcgcagca cgccgaaaaa gcgggcgcgg atgcggttct ggttgtcaca ccttattaca acaagccgaa tcagcgtggc ctctacgagc atttttcgcg cgttgtccgc tctatctcga tcccgctggt gatctataac attccgggcc ggtccattat cgacatgacg ccggaaacca tgggcgctct cgttcgcgac tgtaagaata ttgtcggcgt caaggatgcg accggcaaga tcgagcgtgt ttccgaacag cgcgcgatct gtggcaagga gttcatccag ctttccggtg aagacgccac ggcgcttggc ttcaatgctc atggcggggt ggggtgcatt tcggtaacgt ccaacattgc gccgcgtctt tgcgcggaat ttcaggaagc ctgccaggct ggtaatttcg ccaaggcgct ggaattgcag gaccgtttga tgccgctgca caaggcgctg ttcctggaac ccaatccttc cggcccgaaa tatgcgcttt cgcgcctggg tcgtattgaa aatgttctgc gttcaccaat ggttacgatc gaagcggcga cggcggaaaa gatcgaccat gccatgaagc acgcgggcct catcaactaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for BrsuA.01563.a.A1.PW34594
| PURIFICATION DATe: | 2/9/2012 |
| CONCENTRATION: | 36.84mg/ml |
| OBSERVED MW: | 33kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 9 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLKGSITAL VTPFDREGAF DEKAFRAFVN WQIEEGTKGL VPVGTTGETP TLSHDEHKRV IEVCIEVAAG RVPVIAGAGS NNTVEAIELA QHAEKAGADA VLVVTPYYNK PNQRGLYEHF SRVVRSISIP LVIYNIPGRS IIDMTPETMG ALVRDCKNIV GVKDATGKIE RVSEQRAICG KEFIQLSGED ATALGFNAHG GVGCISVTSN IAPRLCAEFQ EACQAGNFAK ALELQDRLMP LHKALFLEPN PSGPKYALSR LGRIENVLRS PMVTIEAATA EKIDHAMKHA GLIN
Validated NT Sequence
ttattagttg atgaggcccg cgtgcttcat ggcatggtcg atcttttccg ccgtcgccgc ttcgatcgta accattggtg aacgcagaac attttcaata cgacccaggc gcgaaagcgc atatttcggg ccggaaggat tgggttccag gaacagcgcc ttgtgcagcg gcatcaaacg gtcctgcaat tccagcgcct tggcgaaatt accagcctgg caggcttcct gaaattccgc gcaaagacgc ggcgcaatgt tggacgttac cgaaatgcac cccaccccgc catgagcatt gaagccaagc gccgtggcgt cttcaccgga aagctggatg aactccttgc cacagatcgc gcgctgttcg gaaacacgct cgatcttgcc ggtcgcatcc ttgacgccga caatattctt acagtcgcga acgagagcgc ccatggtttc cggcgtcatg tcgataatgg accggcccgg aatgttatag atcaccagcg ggatcgagat agagcggaca acgcgcgaaa aatgctcgta gaggccacgc tgattcggct tgttgtaata aggtgtgaca accagaaccg catccgcgcc cgctttttcg gcgtgctgcg ccagttcgat cgcttccacc gtattgttcg agccggcacc cgcaatcact ggcacacggc cagcggcgac ttcaatgcag acttcgatca cacgcttatg ttcatcatgg gaaagggtcg gcgtttcgcc ggtcgtgcca accggaacga gacctttcgt cccctcttcg atctgccagt tgacgaatgc acgaaaggct ttttcgtcga acgccccttc acggtcgaac ggcgtgacaa gtgcggtaat tgagcccttc agcatcgaac caggaccctg ggtctgagct tccagggtac ccatatgatg gtgatggtga tgagccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLKGSITAL VTPFDREGAF DEKAFRAFVN WQIEEGTKGL VPVGTTGETP TLSHDEHKRV IEVCIEVAAG RVPVIAGAGS NNTVEAIELA QHAEKAGADA VLVVTPYYNK PNQRGLYEHF SRVVRSISIP LVIYNIPGRS IIDMTPETMG ALVRDCKNIV GVKDATGKIE RVSEQRAICG KEFIQLSGED ATALGFNAHG GVGCISVTSN IAPRLCAEFQ EACQAGNFAK ALELQDRLMP LHKALFLEPN PSGPKYALSR LGRIENVLRS PMVTIEAATA EKIDHAMKHA GLIN
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCTGAAGGGC TCAATTACCG CACTTGTCAC GCCGTTCGAC CGTGAAGGGG CGTTCGACGA AAAAGCCTTT CGTGCATTCG TCAACTGGCA GATCGAAGAG GGGACGAAAG GTCTCGTTCC GGTTGGCACG ACCGGCGAAA CGCCGACCCT TTCCCATGAT GAACATAAGC GTGTGATCGA AGTCTGCATT GAAGTCGCCG CTGGCCGTGT GCCAGTGATT GCGGGTGCCG GCTCGAACAA TACGGTGGAA GCGATCGAAC TGGCGCAGCA CGCCGAAAAA GCGGGCGCGG ATGCGGTTCT GGTTGTCACA CCTTATTACA ACAAGCCGAA TCAGCGTGGC CTCTACGAGC ATTTTTCGCG CGTTGTCCGC TCTATCTCGA TCCCGCTGGT GATCTATAAC ATTCCGGGCC GGTCCATTAT CGACATGACG CCGGAAACCA TGGGCGCTCT CGTTCGCGAC TGTAAGAATA TTGTCGGCGT CAAGGATGCG ACCGGCAAGA TCGAGCGTGT TTCCGAACAG CGCGCGATCT GTGGCAAGGA GTTCATCCAG CTTTCCGGTG AAGACGCCAC GGCGCTTGGC TTCAATGCTC ATGGCGGGGT GGGGTGCATT TCGGTAACGT CCAACATTGC GCCGCGTCTT TGCGCGGAAT TTCAGGAAGC CTGCCAGGCT GGTAATTTCG CCAAGGCGCT GGAATTGCAG GACCGTTTGA TGCCGCTGCA CAAGGCGCTG TTCCTGGAAC CCAATCCTTC CGGCCCGAAA TATGCGCTTT CGCGCCTGGG TCGTATTGAA AATGTTCTGC GTTCACCAAT GGTTACGATC GAAGCGGCGA CGGCGGAAAA GATCGACCAT GCCATGAAGC ACGCGGGCCT CATCAACTAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA