BrsuA.01370.a

Phosphomannomutase, putative

CENTER ID: BrsuA.01370.a
ORGANISM: Brucella suis 1330
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrsuA.01370.a.A1.GE34022 full length 1 445
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|204722.5.peg.556
RefSeq: NP_697553.1
UniProt: A0A0H3G6D7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVYVSAFLQH LSHNFSYETV YVGADLRESS PKITISCYRA IELTGRRAIW AGNVPTPALA AYAMARNAPA IMITGSHIPE AYNGIKFYRP DGEFLKDDEA PVRGLAEELL SKVVDGQRSV NLPAPLADVA EEYVSRSIGA FGRDTLAGMK IGIDLHSAVG RDILVRIFKG LGAEVYPFRR TENFVAVDTE ALDPADISRA RTFIAEHGLD AVVSTDGDGD RPLVIDDQGR QVNGDTLGIL TARYLRAKTV VTPLSTTSAL EESGWFENIH RTRIGSPYVV VEMARAVAHP VVGFEANGGF LLGDDVALKT GLLRRLPTRD AVLPAVAVLA QAKDQGMRLS EMVATLPSRF MKADRVKEVP GDRAAPFLHA IETSQSFRSN FSPLIAEPEA ISTVDGVRMA FANGDTVHFR QSGNAPEMRI YIETDSAEKT DRMLSEFIAK LSETI
NT Sequence
atggtgtatg ttagcgcttt cttacagcat ctttctcata atttttcata tgaaacagtt tacgttggag ccgaccttcg ggagtctagc cctaaaataa ccatatcatg ttatagagct attgaactga cgggaaggag agcgatttgg gctggaaatg ttccgacgcc ggcgctcgcg gcttatgcaa tggctcgcaa tgcacctgcc atcatgataa cgggaagcca tatcccagag gcctacaatg gcattaaatt ctatcggcct gacggagaat ttctgaagga cgatgaagca cccgtacgtg gtcttgccga agaattattg agtaaggttg ttgacgggca acggtccgtc aatttgcctg cgcccttggc tgatgttgca gaagaatatg tgagccgttc catcggcgct tttggccggg acacactggc tggaatgaaa atcggaattg atcttcattc cgctgttggg cgagatattc ttgtacgtat atttaaagga cttggagcgg aggtttatcc attccgtaga acggaaaatt tcgttgccgt tgatacagaa gcgcttgatc cagcggatat atctcgggcg cgcacattta tcgccgaaca tgggctcgat gcagtagtgt cgacagatgg agacggtgat aggcctctgg tcattgacga tcagggaaga caggtaaatg gcgatacact gggtattctg acggctcgat atctccgtgc gaaaacagtc gtgacgccgc tcagtacgac gagcgcacta gaggagagtg gctggttcga gaatatccac agaacacgga ttgggtcgcc ctatgttgtg gtcgaaatgg cgcgtgcggt ggcgcatcca gttgttggtt tcgaggcgaa tggcggcttt ctactcggcg acgatgtggc gttgaagact ggtttattgc gccgcctccc aacgcgggat gcagttttgc cagcagtggc tgttcttgcg caggcgaaag accagggtat gcgattgagt gagatggtcg ctacacttcc ttctcgtttc atgaaagccg atagggttaa ggaagttccc ggagaccgcg cagctccatt tttgcacgct atagaaacgt cacaatcatt tcgttctaat ttctccccgt tgattgccga accggaagca atctcgactg tcgacggcgt gcgaatggcg tttgcgaatg gcgatacggt tcatttccga caatcaggca atgctccgga aatgcggatt tatatagaaa cggattccgc ggagaaaact gatcgaatgt tgagcgaatt tattgcaaag ctctctgaaa cgatctga
Details for BrsuA.01370.a.A1.GE34022
HARVESTED ON: 6/4/2012
SEQUENCED ON: 6/5/2012
EXPECTED MW: 50kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 81
PERCENT COVERAGE: 84
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVYVSAFLQ HLSHNFSYET VYVGADLRES SPKITISCYR AIELTGRRAI WAGNVPTPAL AAYAMARNAP AIMITGSHIP EAYNGIKFYR PDGEFLKDDE APVRGLAEEL LSKVVDGQRS VNLPAPLADV AEEYVSRSIG AFGRDTLAGM KIGIDLHSAV GRDILVRIFK GLGAEVYPFR RTENFVAVDT EALDPADISR ARTFIAEHGL DAVVSTDGDG DRPLVIDDXG RQVNGDTLGI LTARYLRAKT VVTPLSTTSA LEESGWFENI HXTRIGSPYV VXEMARAXAH PVVGFEANGG FLLGXXXXXX LXXXAXSQXG MQFCXQXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXX
Validated NT Sequence
ttttnnttac tttaagaagg aganatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatggtgta tgttagcgct ttcttacagc atctttctca taatttttca tatgaaacag tttacgttgg agccgacctt cgggagtcta gccctaaaat aaccatatca tgttatagag ctattgaact gacgggaagg agagcgattt gggctggaaa tgttccgacg ccggcgctcg cggcttatgc aatggctcgc aatgcacctg ccatcatgat aacgggaagc catatcccag aggcctacaa tggcattaaa ttctatcggc ctgacggaga atttctgaag gacgatgaag cacccgtacg tggtcttgcc gaagaattat tgagtaaggt tgttgacggg caacggtccg tcaatttgcc tgcgcccttg gctgatgttg cagaagaata tgtgagccgt tccatcggcg cttttggccg ggacacactg gctggaatga aaatcggaat tgatcttcat tccgctgttg ggcgagatat tcttgtacgt atatttaaag gacttggagc ggaggtttat ccattccgta gaacggaaaa tttcgttgcc gttgatacag aagcgcttga tccagcggat atatctcggg cgcgcacatt tatcgccgaa catgggctcg atgcagtagt gtcgacagat ggagacggtg ataggcctct ggtcattgac gatcanggaa gacaggtaaa tggcgataca ctgggtattc tgacggctcg atatctccgt gcgaaaacag tcgtgacgcc gctcagtacg acgagcgcac tagaggagag tggctggttc gagaatatcc acngaacacg gattgggtcg ccctatgttg tggncgaaat ggcncgtgcg gnggcgcatc cngttgttgg tttcgaggcg aatggcggct ttctactcgg cgannangnn nnttnaanac tggnttnntg ngcnnnctcc caacncggga tgcagttttg ccngcagngn nntntngnnn aannancngn nnngcnntnn nngnnnngnn nnnnnnnnnn nnncnnntnn aannnanngg ntnnnnnnnc ngnnnnncnn nnnnnncnnn tnnnnnnnnn annnnnnnna ncnntnnnnn nnnnnnnnnc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVYVSAFLQ HLSHNFSYET VYVGADLRES SPKITISCYR AIELTGRRAI WAGNVPTPAL AAYAMARNAP AIMITGSHIP EAYNGIKFYR PDGEFLKDDE APVRGLAEEL LSKVVDGQRS VNLPAPLADV AEEYVSRSIG AFGRDTLAGM KIGIDLHSAV GRDILVRIFK GLGAEVYPFR RTENFVAVDT EALDPADISR ARTFIAEHGL DAVVSTDGDG DRPLVIDDQG RQVNGDTLGI LTARYLRAKT VVTPLSTTSA LEESGWFENI HRTRIGSPYV VVEMARAVAH PVVGFEANGG FLLGDDVALK TGLLRRLPTR DAVLPAVAVL AQAKDQGMRL SEMVATLPSR FMKADRVKEV PGDRAAPFLH AIETSQSFRS NFSPLIAEPE AISTVDGVRM AFANGDTVHF RQSGNAPEMR IYIETDSAEK TDRMLSEFIA KLSETI
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgtatgtt agcgctttct tacagcatct ttctcataat ttttcatatg aaacagttta cgttggagcc gaccttcggg agtctagccc taaaataacc atatcatgtt atagagctat tgaactgacg ggaaggagag cgatttgggc tggaaatgtt ccgacgccgg cgctcgcggc ttatgcaatg gctcgcaatg cacctgccat catgataacg ggaagccata tcccagaggc ctacaatggc attaaattct atcggcctga cggagaattt ctgaaggacg atgaagcacc cgtacgtggt cttgccgaag aattattgag taaggttgtt gacgggcaac ggtccgtcaa tttgcctgcg cccttggctg atgttgcaga agaatatgtg agccgttcca tcggcgcttt tggccgggac acactggctg gaatgaaaat cggaattgat cttcattccg ctgttgggcg agatattctt gtacgtatat ttaaaggact tggagcggag gtttatccat tccgtagaac ggaaaatttc gttgccgttg atacagaagc gcttgatcca gcggatatat ctcgggcgcg cacatttatc gccgaacatg ggctcgatgc agtagtgtcg acagatggag acggtgatag gcctctggtc attgacgatc agggaagaca ggtaaatggc gatacactgg gtattctgac ggctcgatat ctccgtgcga aaacagtcgt gacgccgctc agtacgacga gcgcactaga ggagagtggc tggttcgaga atatccacag aacacggatt gggtcgccct atgttgtggt cgaaatggcg cgtgcggtgg cgcatccagt tgttggtttc gaggcgaatg gcggctttct actcggcgac gatgtggcgt tgaagactgg tttattgcgc cgcctcccaa cgcgggatgc agttttgcca gcagtggctg ttcttgcgca ggcgaaagac cagggtatgc gattgagtga gatggtcgct acacttcctt ctcgtttcat gaaagccgat agggttaagg aagttcccgg agaccgcgca gctccatttt tgcacgctat agaaacgtca caatcatttc gttctaattt ctccccgttg attgccgaac cggaagcaat ctcgactgtc gacggcgtgc gaatggcgtt tgcgaatggc gatacggttc atttccgaca atcaggcaat gctccggaaa tgcggattta tatagaaacg gattccgcgg agaaaactga tcgaatgttg agcgaattta ttgcaaagct ctctgaaacg atctaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa