BrsuA.01147.a
Cysteine synthase (EC 2.5.1.47)
| CENTER ID: | BrsuA.01147.a |
| ORGANISM: | Brucella suis 1330 |
| ASSOCIATED DISEASE: | Brucellosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrsuA.01147.a.A1.GE33987 | full length | 1 | 342 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrsuA.01147.a.A1.PS01448 | full length | 1 | 342 |
Structures
5I7W
DEPOSITED: 2/18/2016
DETERMINATION: XRay
CLONE: BrsuA.01147.a.A1.GE33987
PROTEIN: BrsuA.01147.a.A1.PS01448
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_133066 |
| PATRIC ID: | fig|204722.5.peg.1067 |
| RefSeq: | NP_698058.1 |
| UniProt: | A0A0H3G350 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFNSVLDTIG NTPLIRLSKA SELTGCDIYG KAEFLNPGQS VKDRAALYII RDAEKRGLLR PGGVIVEGTA GNTGIGLTMV AKALGYRTAI VIPETQSQEK KDALRLLGAE LIEVPAAPYR NPNNYVRLSG RLAEQLAKTE PNGAIWANQF DNTVNRQAHI ETTAQEIWRD TNDQIDGFVA AVGSGGTLAG TAIGLKERNH NIKIALADPH GAALHAFYTT GELKAEGDSI TEGIGQGRIT ANLEGFTPDF SYQIPDAEAL DILFALVEEE GLCLGGSSGI NIAGAIRLAK DLGPGHTIVT VLCDYGNRYQ SKLFNPAFLR GKSLPVPRWL EKKTEIDIPF EG
NT Sequence
atgttcaatt cggtactcga cacaatcggc aacacgccgt tgatccgcct cagcaaggct tccgagctca caggatgcga tatttacggg aaagcagagt ttctcaatcc gggccagtcg gtcaaggacc gtgcggcgct ctacattatc cgcgatgcgg aaaaacgcgg gcttttgcgg cccggaggcg tgattgtgga aggaaccgcg ggcaataccg gcattggcct cacgatggtc gccaaggcat tgggctaccg cacggcgatc gtgattcctg aaacgcagag ccaggaaaaa aaggacgcct tgcgcctgct cggtgcagag ctgatcgagg ttcccgccgc cccctatcgc aacccgaaca attatgtgcg cctttcaggg cgtcttgccg agcaactggc caaaaccgag ccgaatggcg caatatgggc caatcagttc gacaatacgg tcaaccggca agcccatatc gagaccacgg cgcaggaaat ctggcgcgat acaaacgacc agatcgatgg ttttgtggcc gctgtgggat ctggcggtac gcttgccgga acggcgattg gcctgaagga acggaaccac aatatcaaaa ttgctctggc ggacccgcat ggcgcggcgc ttcacgcctt ctatacgacc ggcgaattga aggcagaagg cgattcgatc accgaaggca tcgggcaggg gcgcatcacc gccaatcttg aaggcttcac ccctgatttt tcctaccaga ttcccgatgc ggaggcgctc gatatcctgt ttgccctcgt ggaagaggag gggctttgcc tcggcggctc gtccggcatc aacattgccg gtgcaatccg gcttgcaaaa gatctcgggc cgggccacac aattgtgacc gtgctttgtg actacggtaa tcgttaccag tcaaagctgt tcaatcccgc cttcctccgc ggcaagagcc tgcccgtgcc gcgctggctg gagaagaaaa ccgaaataga cataccgttc gaggggtaa
Details for BrsuA.01147.a.A1.GE33987
| HARVESTED ON: | 11/21/2011 |
| SEQUENCED ON: | 1/18/2018 |
| EXPECTED MW: | 39kDa |
| OBSERVED MW: | 39kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MGTLEAQTQG PGSMFNSVLD TIGNTPLIRL SKASELTGCD IYGKAEFLNP GQSVKDRAAL YIIRDAEKRG LLRPGGVIVE GTAGNTGIGL TMVAKALGYR TAIVIPETQS QEKKDALRLL GAELIEVPAA PYRNPNNYVR LSGRLAEQLA KTEPNGAIWA NQFDNTVNRQ AHIETTAQEI WRDTNDQIDG FVAAVGSGGT LAGTAIGLKE RNHNIKIALA DPHGAALHAF YTTGELKAEG DSITEGIGQG RITANLEGFT PDFSYQIPDA EALDILFALV EEEGLCLGGS SGINIAGAIR LAKDLGPGHT IVTVLCDYGN RYQSKLFNPA FLRGKSLPVP RWLEKKTEID IPF
Validated NT Sequence
cgaacggtat gtctatttcg gttttcttct ccagccagcg cggcacgggc aggctcttgc cgcggaggaa ggcgggattg aacagctttg actggtaacg attaccgtag tcacaaagca cggtcacaat tgtgtggccc ggcccgagat cttttgcaag ccggattgca ccggcaatgt tgatgccgga cgagccgccg aggcaaagcc cctcctcttc cacgagggca aacaggatat cgagcgcctc cgcatcggga atctggtagg aaaaatcagg ggtgaagcct tcaagattgg cggtgatgcg cccctgcccg atgccttcgg tgatcgaatc gccttctgcc ttcaattcgc cggtcgtata gaaggcgtga agcgccgcgc catgcgggtc cgccagagca attttgatat tgtggttccg ttccttcagg ccaatcgccg ttccggcaag cgtaccgcca gatcccacag cggccacaaa accatcgatc tggtcgtttg tatcgcgcca gatttcctgc gccgtggtct cgatatgggc ttgccggttg accgtattgt cgaactgatt ggcccatatt gcgccattcg gctcggtttt ggccagttgc tcggcaagac gccctgaaag gcgcacataa ttgttcgggt tgcgataggg ggcggcggga acctcgatca gctctgcacc gagcaggcgc aaggcgtcct ttttttcctg gctctgcgtt tcaggaatca cgatcgccgt gcggtagccc aatgccttgg cgaccatcgt gaggccaatg ccggtattgc ccgcggttcc ttccacaatc acgcctccgg gccgcaaaag cccgcgtttt tccgcatcgc ggataatgta gagcgccgca cggtccttga ccgactggcc cggattgaga aactctgctt tcccgtaaat atcgcatcct gtgagctcgg aagccttgct gaggcggatc aacggcgtgt tgccgattgt gtcgagtacc gaattgaaca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tggtgatgag cc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMFNSVLDTI GNTPLIRLSK ASELTGCDIY GKAEFLNPGQ SVKDRAALYI IRDAEKRGLL RPGGVIVEGT AGNTGIGLTM VAKALGYRTA IVIPETQSQE KKDALRLLGA ELIEVPAAPY RNPNNYVRLS GRLAEQLAKT EPNGAIWANQ FDNTVNRQAH IETTAQEIWR DTNDQIDGFV AAVGSGGTLA GTAIGLKERN HNIKIALADP HGAALHAFYT TGELKAEGDS ITEGIGQGRI TANLEGFTPD FSYQIPDAEA LDILFALVEE EGLCLGGSSG INIAGAIRLA KDLGPGHTIV TVLCDYGNRY QSKLFNPAFL RGKSLPVPRW LEKKTEIDIP FEG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttcaattcg gtactcgaca caatcggcaa cacgccgttg atccgcctca gcaaggcttc cgagctcaca ggatgcgata tttacgggaa agcagagttt ctcaatccgg gccagtcggt caaggaccgt gcggcgctct acattatccg cgatgcggaa aaacgcgggc ttttgcggcc cggaggcgtg attgtggaag gaaccgcggg caataccggc attggcctca cgatggtcgc caaggcattg ggctaccgca cggcgatcgt gattcctgaa acgcagagcc aggaaaaaaa ggacgccttg cgcctgctcg gtgcagagct gatcgaggtt cccgccgccc cctatcgcaa cccgaacaat tatgtgcgcc tttcagggcg tcttgccgag caactggcca aaaccgagcc gaatggcgca atatgggcca atcagttcga caatacggtc aaccggcaag cccatatcga gaccacggcg caggaaatct ggcgcgatac aaacgaccag atcgatggtt ttgtggccgc tgtgggatct ggcggtacgc ttgccggaac ggcgattggc ctgaaggaac ggaaccacaa tatcaaaatt gctctggcgg acccgcatgg cgcggcgctt cacgccttct atacgaccgg cgaattgaag gcagaaggcg attcgatcac cgaaggcatc gggcaggggc gcatcaccgc caatcttgaa ggcttcaccc ctgatttttc ctaccagatt cccgatgcgg aggcgctcga tatcctgttt gccctcgtgg aagaggaggg gctttgcctc ggcggctcgt ccggcatcaa cattgccggt gcaatccggc ttgcaaaaga tctcgggccg ggccacacaa ttgtgaccgt gctttgtgac tacggtaatc gttaccagtc aaagctgttc aatcccgcct tcctccgcgg caagagcctg cccgtgccgc gctggctgga gaagaaaacc gaaatagaca taccgttcga ggggtaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for BrsuA.01147.a.A1.PS01448
| PURIFICATION DATe: | 4/3/2012 |
| CONCENTRATION: | 43.62mg/ml |
| OBSERVED MW: | 38kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 4 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMFNSVLD TIGNTPLIRL SKASELTGCD IYGKAEFLNP GQSVKDRAAL YIIRDAEKRG LLRPGGVIVE GTAGNTGIGL TMVAKALGYR TAIVIPETQS QEKKDALRLL GAELIEVPAA PYRNPNNYVR LSGRLAEQLA KTEPNGAIWA NQFDNTVNRQ AHIETTAQEI WRDTNDQIDG FVAAVGSGGT LAGTAIGLKE RNHNIKIALA DPHGAALHAF YTTGELKAEG DSITEGIGQG RITANLEGFT PDFSYQIPDA EALDILFALV EEEGLCLGGS SGINIAGAIR LAKDLGPGHT IVTVLCDYGN RYQSKLFNPA FLRGKSLPVP RWLEKKTEID IPF
Validated NT Sequence
cgaacggtat gtctatttcg gttttcttct ccagccagcg cggcacgggc aggctcttgc cgcggaggaa ggcgggattg aacagctttg actggtaacg attaccgtag tcacaaagca cggtcacaat tgtgtggccc ggcccgagat cttttgcaag ccggattgca ccggcaatgt tgatgccgga cgagccgccg aggcaaagcc cctcctcttc cacgagggca aacaggatat cgagcgcctc cgcatcggga atctggtagg aaaaatcagg ggtgaagcct tcaagattgg cggtgatgcg cccctgcccg atgccttcgg tgatcgaatc gccttctgcc ttcaattcgc cggtcgtata gaaggcgtga agcgccgcgc catgcgggtc cgccagagca attttgatat tgtggttccg ttccttcagg ccaatcgccg ttccggcaag cgtaccgcca gatcccacag cggccacaaa accatcgatc tggtcgtttg tatcgcgcca gatttcctgc gccgtggtct cgatatgggc ttgccggttg accgtattgt cgaactgatt ggcccatatt gcgccattcg gctcggtttt ggccagttgc tcggcaagac gccctgaaag gcgcacataa ttgttcgggt tgcgataggg ggcggcggga acctcgatca gctctgcacc gagcaggcgc aaggcgtcct ttttttcctg gctctgcgtt tcaggaatca cgatcgccgt gcggtagccc aatgccttgg cgaccatcgt gaggccaatg ccggtattgc ccgcggttcc ttccacaatc acgcctccgg gccgcaaaag cccgcgtttt tccgcatcgc ggataatgta gagcgccgca cggtccttga ccgactggcc cggattgaga aactctgctt tcccgtaaat atcgcatcct gtgagctcgg aagccttgct gaggcggatc aacggcgtgt tgccgattgt gtcgagtacc gaattgaaca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tggtgatgag cc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMFNSVLDTI GNTPLIRLSK ASELTGCDIY GKAEFLNPGQ SVKDRAALYI IRDAEKRGLL RPGGVIVEGT AGNTGIGLTM VAKALGYRTA IVIPETQSQE KKDALRLLGA ELIEVPAAPY RNPNNYVRLS GRLAEQLAKT EPNGAIWANQ FDNTVNRQAH IETTAQEIWR DTNDQIDGFV AAVGSGGTLA GTAIGLKERN HNIKIALADP HGAALHAFYT TGELKAEGDS ITEGIGQGRI TANLEGFTPD FSYQIPDAEA LDILFALVEE EGLCLGGSSG INIAGAIRLA KDLGPGHTIV TVLCDYGNRY QSKLFNPAFL RGKSLPVPRW LEKKTEIDIP FEG
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTTCAATTCG GTACTCGACA CAATCGGCAA CACGCCGTTG ATCCGCCTCA GCAAGGCTTC CGAGCTCACA GGATGCGATA TTTACGGGAA AGCAGAGTTT CTCAATCCGG GCCAGTCGGT CAAGGACCGT GCGGCGCTCT ACATTATCCG CGATGCGGAA AAACGCGGGC TTTTGCGGCC CGGAGGCGTG ATTGTGGAAG GAACCGCGGG CAATACCGGC ATTGGCCTCA CGATGGTCGC CAAGGCATTG GGCTACCGCA CGGCGATCGT GATTCCTGAA ACGCAGAGCC AGGAAAAAAA GGACGCCTTG CGCCTGCTCG GTGCAGAGCT GATCGAGGTT CCCGCCGCCC CCTATCGCAA CCCGAACAAT TATGTGCGCC TTTCAGGGCG TCTTGCCGAG CAACTGGCCA AAACCGAGCC GAATGGCGCA ATATGGGCCA ATCAGTTCGA CAATACGGTC AACCGGCAAG CCCATATCGA GACCACGGCG CAGGAAATCT GGCGCGATAC AAACGACCAG ATCGATGGTT TTGTGGCCGC TGTGGGATCT GGCGGTACGC TTGCCGGAAC GGCGATTGGC CTGAAGGAAC GGAACCACAA TATCAAAATT GCTCTGGCGG ACCCGCATGG CGCGGCGCTT CACGCCTTCT ATACGACCGG CGAATTGAAG GCAGAAGGCG ATTCGATCAC CGAAGGCATC GGGCAGGGGC GCATCACCGC CAATCTTGAA GGCTTCACCC CTGATTTTTC CTACCAGATT CCCGATGCGG AGGCGCTCGA TATCCTGTTT GCCCTCGTGG AAGAGGAGGG GCTTTGCCTC GGCGGCTCGT CCGGCATCAA CATTGCCGGT GCAATCCGGC TTGCAAAAGA TCTCGGGCCG GGCCACACAA TTGTGACCGT GCTTTGTGAC TACGGTAATC GTTACCAGTC AAAGCTGTTC AATCCCGCCT TCCTCCGCGG CAAGAGCCTG CCCGTGCCGC GCTGGCTGGA GAAGAAAACC GAAATAGACA TACCGTTCGA GGGGTAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA