BrsuA.01141.b

Fructokinase (EC 2.7.1.4)

CENTER ID: BrsuA.01141.b
ORGANISM: Brucella suis 1330
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrsuA.01141.b.B1.GE36775 Full length( BrsuA.01141.b ) 1 308
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrsuA.01141.b.B1.PW37372 Full length( BrsuA.01141.b ) 1 308

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruSui107850_0119
BV-BRC: fig|204722.5.peg.119
RefSeq: NP_697156.1
RefSeq: YP_005614975.1
UniProt: A0A0H3G5B6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MILCCGEALI DMLPRETTGG ETAFQPFAGG SVFNTAIALG RLGVPTGFFS GISSDFFGDV LRDTLARSNV DYSFAAISNR PTTLAFVRLV DGQARYAFYD ENTAGRMLSR NDMPYVDETI SAMLFGCISL ISEPCGSVYE TLLAREAPNR VMFLDPNIRA NLITVRETHL TRMKRMIALA DIVKLSDEDL DWFGEKGSHD EIAAEWLKLG PKLVVITKGA HGAVVYTNHA TVPVPGVKVD VVDTVGAGDT VNAGILASLH SQGLLTKDAL ANLSEDQIHS AVALGVRAAA VTVSRAGANP PWAHEMRD
NT Sequence
ATGATCCTTT GTTGCGGTGA AGCCCTGATC GACATGCTGC CACGCGAAAC CACTGGCGGC GAAACCGCAT TCCAGCCTTT CGCCGGCGGT TCGGTTTTCA ACACGGCCAT TGCGCTGGGG CGGCTTGGCG TTCCGACCGG CTTTTTCTCC GGCATTTCAT CGGATTTTTT CGGCGATGTG CTGCGCGACA CGCTGGCGCG CTCCAATGTC GATTATTCCT TCGCCGCCAT TTCCAACAGG CCGACAACGC TTGCCTTCGT GCGCCTCGTG GACGGTCAGG CACGCTATGC TTTCTATGAC GAAAACACGG CCGGGCGAAT GCTGTCGAGA AACGACATGC CCTATGTGGA TGAAACCATA AGCGCCATGC TGTTCGGCTG CATCAGCCTT ATTTCCGAAC CCTGCGGCAG CGTCTATGAG ACGCTGCTGG CGCGGGAAGC ACCAAACCGG GTGATGTTCC TCGACCCCAA TATTCGCGCA AACCTCATCA CCGTCCGTGA AACGCACCTG ACGCGCATGA AGCGCATGAT CGCGCTTGCC GATATCGTAA AACTCTCCGA CGAAGACCTG GACTGGTTCG GGGAAAAAGG CAGCCATGAC GAGATTGCCG CCGAATGGCT GAAGCTTGGG CCGAAACTTG TCGTCATCAC CAAGGGCGCA CATGGCGCTG TTGTCTATAC AAACCATGCA ACCGTGCCTG TGCCGGGCGT GAAGGTGGAT GTGGTTGATA CGGTGGGCGC GGGCGACACG GTCAATGCGG GAATTCTGGC GAGCCTCCAC AGTCAGGGCC TTCTGACGAA GGACGCGCTT GCGAACCTGA GCGAAGACCA GATACATTCC GCCGTGGCGC TCGGGGTACG GGCTGCTGCC GTGACCGTCT CGCGCGCAGG TGCGAACCCG CCATGGGCGC ATGAGATGCG GGAT
Details for BrsuA.01141.b.B1.GE36775
HARVESTED ON: 3/27/2013
SEQUENCED ON: 3/30/2013
EXPECTED MW: 34kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMI LCCGEALIDM LPRETTGGET AFQPFAGGSV FNTAIALGRL GVPTGFFSGI SSDFFGDVLR DTLARSNVDY SFAAISNRPT TLAFVRLVDG QARYAFYDEN TAGRMLSRND MPYVDETISA MLFGCISLIS EPCGSVYETL LAREAPNRVM FLDPNIRANL ITVRETHLTR MKRMIALADI VKLSDEDLDW FGEKGSHDEI AAEWLKLGPK LVVITKGAHG AVVYTNHATV PVPGVKVDVV DTVGAGDTVN AGILASLHSQ GLLTKDALAN LSEDQIHSAV ALGVRAAAVT VSRAGANPPW AHEMRD
Validated NT Sequence
ccggntccta tcttactcac ttaatcccgc atctcatgcg cccatggcgg gttcgcacct gcgcgcgaga cggtcacggc agcagcccgt accccgagcg ccacggcgga atgtatctgg tcttcgctca ggttcgcaag cgcgtccttc gtcagaaggc cctgactgtg gaggctcgcc agaattcccg cattgaccgt gtcgcccgcg cccaccgtat caaccacatc caccttcacg cccggcacag gcacggttgc atggtttgta tagacaacag cgccatgtgc gcccttggtg atgacgacaa gtttcggccc aagcttcagc cattcggcgg caatctcgtc atggctgcct ttttccccga accagtccag gtcttcgtcg gagagtttta cgatatcggc aagcgcgatc atgcgcttca tgcgcgtcag gtgcgtttca cggacggtga tgaggtttgc gcgaatattg gggtcgagga acatcacccg gtttggtgct tcccgcgcca gcagcgtctc atagacgctg ccgcagggtt cggaaataag gctgatgcag ccgaacagca tggcgcttat ggtttcatcc acatagggca tgtcgtttct cgacagcatt cgcccggccg tgttttcgtc atagaaagca tagcgtgcct gaccgtccac gaggcgcacg aaggcaagcg ttgtcggcct gttggaaatg gcggcgaagg aataatcgac attggagcgc gccagcgtgt cgcgcagcac atcgccgaaa aaatccgatg aaatgccgga gaaaaagccg gtcggaacgc caagccgccc cagcgcaatg gccgtgttga aaaccgaacc gccggcgaaa ggctggaatg cggtttcgcc gccagtggtt tcgcgtggca gcatgtcgat cagggcttca ccgcaacaaa ggatcatatg gtggtggtgg tggtgagcca t
Expected Protein Sequence
MAHHHHHHMI LCCGEALIDM LPRETTGGET AFQPFAGGSV FNTAIALGRL GVPTGFFSGI SSDFFGDVLR DTLARSNVDY SFAAISNRPT TLAFVRLVDG QARYAFYDEN TAGRMLSRND MPYVDETISA MLFGCISLIS EPCGSVYETL LAREAPNRVM FLDPNIRANL ITVRETHLTR MKRMIALADI VKLSDEDLDW FGEKGSHDEI AAEWLKLGPK LVVITKGAHG AVVYTNHATV PVPGVKVDVV DTVGAGDTVN AGILASLHSQ GLLTKDALAN LSEDQIHSAV ALGVRAAAVT VSRAGANPPW AHEMRD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgatcc tttgttgcgg tgaagccctg atcgacatgc tgccacgcga aaccactggc ggcgaaaccg cattccagcc tttcgccggc ggttcggttt tcaacacggc cattgcgctg gggcggcttg gcgttccgac cggctttttc tccggcattt catcggattt tttcggcgat gtgctgcgcg acacgctggc gcgctccaat gtcgattatt ccttcgccgc catttccaac aggccgacaa cgcttgcctt cgtgcgcctc gtggacggtc aggcacgcta tgctttctat gacgaaaaca cggccgggcg aatgctgtcg agaaacgaca tgccctatgt ggatgaaacc ataagcgcca tgctgttcgg ctgcatcagc cttatttccg aaccctgcgg cagcgtctat gagacgctgc tggcgcggga agcaccaaac cgggtgatgt tcctcgaccc caatattcgc gcaaacctca tcaccgtccg tgaaacgcac ctgacgcgca tgaagcgcat gatcgcgctt gccgatatcg taaaactctc cgacgaagac ctggactggt tcggggaaaa aggcagccat gacgagattg ccgccgaatg gctgaagctt gggccgaaac ttgtcgtcat caccaagggc gcacatggcg ctgttgtcta tacaaaccat gcaaccgtgc ctgtgccggg cgtgaaggtg gatgtggttg atacggtggg cgcgggcgac acggtcaatg cgggaattct ggcgagcctc cacagtcagg gccttctgac gaaggacgcg cttgcgaacc tgagcgaaga ccagatacat tccgccgtgg cgctcggggt acgggctgct gccgtgaccg tctcgcgcgc aggtgcgaac ccgccatggg cgcatgagat gcgggattga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for BrsuA.01141.b.B1.PW37372
PURIFICATION DATe: 10/1/2013
CONCENTRATION: 16.6mg/ml
OBSERVED MW: 33kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMI LCCGEALIDM LPRETTGGET AFQPFAGGSV FNTAIALGRL GVPTGFFSGI SSDFFGDVLR DTLARSNVDY SFAAISNRPT TLAFVRLVDG QARYAFYDEN TAGRMLSRND MPYVDETISA MLFGCISLIS EPCGSVYETL LAREAPNRVM FLDPNIRANL ITVRETHLTR MKRMIALADI VKLSDEDLDW FGEKGSHDEI AAEWLKLGPK LVVITKGAHG AVVYTNHATV PVPGVKVDVV DTVGAGDTVN AGILASLHSQ GLLTKDALAN LSEDQIHSAV ALGVRAAAVT VSRAGANPPW AHEMRD
Validated NT Sequence
ccggntccta tcttactcac ttaatcccgc atctcatgcg cccatggcgg gttcgcacct gcgcgcgaga cggtcacggc agcagcccgt accccgagcg ccacggcgga atgtatctgg tcttcgctca ggttcgcaag cgcgtccttc gtcagaaggc cctgactgtg gaggctcgcc agaattcccg cattgaccgt gtcgcccgcg cccaccgtat caaccacatc caccttcacg cccggcacag gcacggttgc atggtttgta tagacaacag cgccatgtgc gcccttggtg atgacgacaa gtttcggccc aagcttcagc cattcggcgg caatctcgtc atggctgcct ttttccccga accagtccag gtcttcgtcg gagagtttta cgatatcggc aagcgcgatc atgcgcttca tgcgcgtcag gtgcgtttca cggacggtga tgaggtttgc gcgaatattg gggtcgagga acatcacccg gtttggtgct tcccgcgcca gcagcgtctc atagacgctg ccgcagggtt cggaaataag gctgatgcag ccgaacagca tggcgcttat ggtttcatcc acatagggca tgtcgtttct cgacagcatt cgcccggccg tgttttcgtc atagaaagca tagcgtgcct gaccgtccac gaggcgcacg aaggcaagcg ttgtcggcct gttggaaatg gcggcgaagg aataatcgac attggagcgc gccagcgtgt cgcgcagcac atcgccgaaa aaatccgatg aaatgccgga gaaaaagccg gtcggaacgc caagccgccc cagcgcaatg gccgtgttga aaaccgaacc gccggcgaaa ggctggaatg cggtttcgcc gccagtggtt tcgcgtggca gcatgtcgat cagggcttca ccgcaacaaa ggatcatatg gtggtggtgg tggtgagcca t
Expressed Protein Sequence
MAHHHHHHMI LCCGEALIDM LPRETTGGET AFQPFAGGSV FNTAIALGRL GVPTGFFSGI SSDFFGDVLR DTLARSNVDY SFAAISNRPT TLAFVRLVDG QARYAFYDEN TAGRMLSRND MPYVDETISA MLFGCISLIS EPCGSVYETL LAREAPNRVM FLDPNIRANL ITVRETHLTR MKRMIALADI VKLSDEDLDW FGEKGSHDEI AAEWLKLGPK LVVITKGAHG AVVYTNHATV PVPGVKVDVV DTVGAGDTVN AGILASLHSQ GLLTKDALAN LSEDQIHSAV ALGVRAAAVT VSRAGANPPW AHEMRD
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGATCC TTTGTTGCGG TGAAGCCCTG ATCGACATGC TGCCACGCGA AACCACTGGC GGCGAAACCG CATTCCAGCC TTTCGCCGGC GGTTCGGTTT TCAACACGGC CATTGCGCTG GGGCGGCTTG GCGTTCCGAC CGGCTTTTTC TCCGGCATTT CATCGGATTT TTTCGGCGAT GTGCTGCGCG ACACGCTGGC GCGCTCCAAT GTCGATTATT CCTTCGCCGC CATTTCCAAC AGGCCGACAA CGCTTGCCTT CGTGCGCCTC GTGGACGGTC AGGCACGCTA TGCTTTCTAT GACGAAAACA CGGCCGGGCG AATGCTGTCG AGAAACGACA TGCCCTATGT GGATGAAACC ATAAGCGCCA TGCTGTTCGG CTGCATCAGC CTTATTTCCG AACCCTGCGG CAGCGTCTAT GAGACGCTGC TGGCGCGGGA AGCACCAAAC CGGGTGATGT TCCTCGACCC CAATATTCGC GCAAACCTCA TCACCGTCCG TGAAACGCAC CTGACGCGCA TGAAGCGCAT GATCGCGCTT GCCGATATCG TAAAACTCTC CGACGAAGAC CTGGACTGGT TCGGGGAAAA AGGCAGCCAT GACGAGATTG CCGCCGAATG GCTGAAGCTT GGGCCGAAAC TTGTCGTCAT CACCAAGGGC GCACATGGCG CTGTTGTCTA TACAAACCAT GCAACCGTGC CTGTGCCGGG CGTGAAGGTG GATGTGGTTG ATACGGTGGG CGCGGGCGAC ACGGTCAATG CGGGAATTCT GGCGAGCCTC CACAGTCAGG GCCTTCTGAC GAAGGACGCG CTTGCGAACC TGAGCGAAGA CCAGATACAT TCCGCCGTGG CGCTCGGGGT ACGGGCTGCT GCCGTGACCG TCTCGCGCGC AGGTGCGAAC CCGCCATGGG CGCATGAGAT GCGGGATTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT