BrsuA.01123.b
Dihydropyrimidinase (EC 3.5.2.2)
| CENTER ID: | BrsuA.01123.b |
| ORGANISM: | Brucella suis 1330 |
| ASSOCIATED DISEASE: | Brucellosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
Clicking the button will add the material to a virtual cart.
You may order multiple materials at a time at no cost to you, as this contract is funded
by NIAID. When you are ready to place your order, click the "Place Order" link which will
appear in the top right corner of the page after you place your first item in your cart.
Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrsuA.01123.b.B1.GE36774 | Full length( BrsuA.01123.b ) | 1 | 489 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrsuA.01123.b.B1.PS01876 | Full length( BrsuA.01123.b ) | 1 | 489 |
Structures
4TQT
DEPOSITED: 6/12/2014
DETERMINATION: XRay
CLONE: BrsuA.01123.b.B1.GE36774
PROTEIN: BrsuA.01123.b.B1.PS01876
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|204722.5.peg.291 |
| RefSeq: | NP_697312.1 |
| RefSeq: | YP_005615132.1 |
| UniProt: | A0A0H3G9X2 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAKTGASKVI KGGTVITADR TFRADILIED GKIAAIGDSL EGDEVIDASG CYVMPGGIDP HTHLQMPFMG TYSSDDFDTG TAAALAGGTT MVVDFVLPDS EGNLLDALQE WFQKAGKART DYSFHMAITG WNERTFNEMA EVVKRGINTF KHFMAYKGAL MVNDDEMFAS FQRCAELGAM PLVHAENGDI VAQLQAKLMA EGNDGPEAHA YSRPPEVEGE ATNRAIMIAD QAGVPLYVVH VSCEQSHEAI RRARQKGMRV FGEPLIQHLT LDESEYHNRD WDYAARRVMS PPFRDKLNQD SLWAGLAAGS LQCVATDHCA FTTEQKRYGI GNFTKIPNGT GGLEERMPVL WTRGVRTGRL TPNEFVAVTS TNIAKILNIY PQKGAVVPGA DADLVIWDPE TTKKISAKTQ HSSIDYNVFE GFELKGLPIM TLSRGRIAFD KGQVTAKPGD GRFIEREPNG AVNRALSQWK EIVAPRKVER SAEHMPIGV
NT Sequence
ATGGCCAAGA CCGGGGCAAG CAAAGTCATC AAGGGCGGCA CCGTCATCAC GGCTGACCGC ACGTTCAGGG CAGATATTCT TATCGAAGAC GGCAAGATCG CCGCCATTGG CGATAGCCTT GAAGGCGATG AGGTCATCGA CGCATCCGGC TGCTATGTCA TGCCGGGCGG CATCGACCCG CACACCCACC TGCAAATGCC CTTCATGGGC ACATATTCCT CCGACGATTT CGACACGGGC ACGGCGGCAG CACTTGCCGG CGGCACGACG ATGGTGGTCG ATTTCGTCCT GCCGGATTCC GAGGGCAATC TTCTGGATGC GCTTCAGGAA TGGTTTCAGA AGGCGGGCAA GGCGCGCACC GATTATTCGT TCCACATGGC GATCACCGGC TGGAACGAGC GCACTTTCAA CGAAATGGCC GAAGTGGTGA AGCGCGGCAT CAACACGTTC AAGCATTTCA TGGCCTATAA GGGCGCGCTG ATGGTGAATG ACGATGAGAT GTTCGCCTCG TTCCAGCGTT GTGCGGAACT GGGTGCCATG CCGCTCGTCC ATGCGGAAAA CGGCGATATC GTCGCGCAAT TGCAGGCGAA ACTCATGGCC GAAGGCAATG ACGGGCCGGA AGCCCATGCC TATTCCCGCC CGCCGGAAGT GGAAGGAGAA GCCACCAATC GCGCCATCAT GATCGCCGAT CAGGCGGGCG TACCGCTTTA TGTGGTGCAT GTCTCCTGCG AGCAAAGCCA TGAAGCAATC CGCCGCGCGC GCCAGAAGGG TATGCGTGTT TTCGGCGAGC CGCTGATCCA GCATCTGACG CTCGATGAAA GCGAATATCA CAATCGCGAC TGGGACTATG CCGCGCGGCG CGTGATGTCG CCGCCGTTCC GCGACAAGCT CAATCAGGAC AGCCTCTGGG CGGGCCTTGC GGCTGGAAGC CTGCAATGCG TGGCGACCGA TCATTGCGCC TTCACCACCG AGCAGAAACG CTATGGCATC GGCAATTTCA CCAAGATTCC GAACGGCACC GGCGGGCTGG AGGAACGTAT GCCGGTTCTG TGGACGCGCG GCGTGCGAAC GGGCCGCCTG ACACCTAATG AGTTCGTGGC CGTCACTTCC ACGAACATCG CGAAAATCCT CAACATCTAT CCGCAGAAAG GTGCCGTGGT GCCAGGCGCG GACGCCGATC TTGTTATATG GGATCCGGAG ACGACCAAGA AGATTTCCGC CAAGACGCAG CATTCCTCCA TCGATTACAA CGTGTTCGAG GGTTTCGAGC TGAAGGGGCT GCCGATCATG ACGCTGTCGC GCGGGCGGAT CGCCTTCGAC AAGGGACAGG TGACAGCAAA ACCGGGCGAC GGGCGCTTCA TCGAGCGTGA GCCGAATGGC GCGGTCAATC GCGCGCTATC GCAATGGAAG GAAATTGTCG CGCCGCGCAA GGTGGAGCGC AGTGCCGAAC ATATGCCGAT AGGAGTC
Details for BrsuA.01123.b.B1.GE36774
| HARVESTED ON: | 3/27/2013 |
| SEQUENCED ON: | 3/30/2013 |
| EXPECTED MW: | 55kDa |
| OBSERVED MW: | 55kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 85 |
Validated AA Sequence
MPGGIDPHTH LQMPFMGTYS SDDFDTGTAA ALAGGTTMVV DFVLPDSEGN LLDALQEWFQ KAGKARTDYS FHMAITGWNE RTFNEMAEVV KRGINTFKHF MAYKGALMVN DDEMFASFQR CAELGAMPLV HAENGDIVAQ LQAKLMAEGN DGPEAHAYSR PPEVEGEATN RAIMIADQAG VPLYVVHVSC EQSHEAIRRA RQKGMRVFGE PLIQHLTLXE SEYHNRDWDY AARRVMSPPF RDKLNQDSLW AGLAAGSLQC VATDHCAFTT EQKRYGIGNF TKIPNGTGGL EERMPVLWTR GVRTGRLTPN EFVAVTSTNI AKILNIYPQK GAVVPGADAD LVIWDPETTK KISAKTQHSS IDYNVFEGFE LKGLPIMTLS RGRIAFDKGQ VTAKPGDGRF IEREPNGAVN RALSQWKEIV APRKVE
Validated NT Sequence
cgctccacct tgcgcggcgc gacaatttcc ttccattgcg atagcgcgcg attgaccgcg ccattcggct cacgctcgat gaagcgcccg tcgcccggtt ttgctgtcac ctgtcccttg tcgaaggcga tccgcccgcg cgacagcgtc atgatcggca gccccttcag ctcgaaaccc tcgaacacgt tgtaatcgat ggaggaatgc tgcgtcttgg cggaaatctt cttggtcgtc tccggatccc atataacaag atcggcgtcc gcgcctggca ccacggcacc tttctgcgga tagatgttga ggattttcgc gatgttcgtg gaagtgacgg ccacgaactc attaggtgtc aggcggcccg ttcgcacgcc gcgcgtccac agaaccggca tacgttcctc cagcccgccg gtgccgttcg gaatcttggt gaaattgccg atgccatagc gtttctgctc ggtggtgaag gcgcaatgat cggtcgccac gcattgcagg cttccagccg caaggcccgc ccagaggctg tcctgattga gcttgtcgcg gaacggcggc gacatcacgc gccgcgcggc atagtcccag tcgcgattgt gatattcgct ttcntcgagc gtcagatgct ggatcagcgg ctcgccgaaa acacgcatac ccttctggcg cgcgcggcgg attgcttcat ggctttgctc gcaggagaca tgcaccacat aaagcggtac gcccgcctga tcggcgatca tgatggcgcg attggtggct tctccttcca cttccggcgg gcgggaatag gcatgggctt ccggcccgtc attgccttcg gccatgagtt tcgcctgcaa ttgcgcgacg atatcgccgt tttccgcatg gacgagcggc atggcaccca gttccgcaca acgctggaac gaggcgaaca tctcatcgtc attcaccatc agcgcgccct tataggccat gaaatgcttg aacgtgttga tgccgcgctt caccacttcg gccatttcgt tgaaagtgcg ctcgttccag ccggtgatcg ccatgtggaa cgaataatcg gtgcgcgcct tgcccgcctt ctgaaaccat tcctgaagcg catccagaag attgccctcg gaatccggca ggacgaaatc gaccaccatc gtcgtgccgc cggcaagtgc tgccgccgtg cccgtgtcga aatcgtcgga ggaatatgtg cccatgaagg gcatttgcag gtgggtgtgc gggtcgatgc cgcccggcat gacatagcag ccggatgcgt cgatgacctc atcgccttca aggctatcgc caatggcggc gatcttgccg tcttcgataa gaatatctgc cctgaacgtg cggtcagccg tgatgacggt gccgcccttg atgactttgc ttgccccggt c
Expected Protein Sequence
MAHHHHHHMA KTGASKVIKG GTVITADRTF RADILIEDGK IAAIGDSLEG DEVIDASGCY VMPGGIDPHT HLQMPFMGTY SSDDFDTGTA AALAGGTTMV VDFVLPDSEG NLLDALQEWF QKAGKARTDY SFHMAITGWN ERTFNEMAEV VKRGINTFKH FMAYKGALMV NDDEMFASFQ RCAELGAMPL VHAENGDIVA QLQAKLMAEG NDGPEAHAYS RPPEVEGEAT NRAIMIADQA GVPLYVVHVS CEQSHEAIRR ARQKGMRVFG EPLIQHLTLD ESEYHNRDWD YAARRVMSPP FRDKLNQDSL WAGLAAGSLQ CVATDHCAFT TEQKRYGIGN FTKIPNGTGG LEERMPVLWT RGVRTGRLTP NEFVAVTSTN IAKILNIYPQ KGAVVPGADA DLVIWDPETT KKISAKTQHS SIDYNVFEGF ELKGLPIMTL SRGRIAFDKG QVTAKPGDGR FIEREPNGAV NRALSQWKEI VAPRKVERSA EHMPIGV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcca agaccggggc aagcaaagtc atcaagggcg gcaccgtcat cacggctgac cgcacgttca gggcagatat tcttatcgaa gacggcaaga tcgccgccat tggcgatagc cttgaaggcg atgaggtcat cgacgcatcc ggctgctatg tcatgccggg cggcatcgac ccgcacaccc acctgcaaat gcccttcatg ggcacatatt cctccgacga tttcgacacg ggcacggcgg cagcacttgc cggcggcacg acgatggtgg tcgatttcgt cctgccggat tccgagggca atcttctgga tgcgcttcag gaatggtttc agaaggcggg caaggcgcgc accgattatt cgttccacat ggcgatcacc ggctggaacg agcgcacttt caacgaaatg gccgaagtgg tgaagcgcgg catcaacacg ttcaagcatt tcatggccta taagggcgcg ctgatggtga atgacgatga gatgttcgcc tcgttccagc gttgtgcgga actgggtgcc atgccgctcg tccatgcgga aaacggcgat atcgtcgcgc aattgcaggc gaaactcatg gccgaaggca atgacgggcc ggaagcccat gcctattccc gcccgccgga agtggaagga gaagccacca atcgcgccat catgatcgcc gatcaggcgg gcgtaccgct ttatgtggtg catgtctcct gcgagcaaag ccatgaagca atccgccgcg cgcgccagaa gggtatgcgt gttttcggcg agccgctgat ccagcatctg acgctcgatg aaagcgaata tcacaatcgc gactgggact atgccgcgcg gcgcgtgatg tcgccgccgt tccgcgacaa gctcaatcag gacagcctct gggcgggcct tgcggctgga agcctgcaat gcgtggcgac cgatcattgc gccttcacca ccgagcagaa acgctatggc atcggcaatt tcaccaagat tccgaacggc accggcgggc tggaggaacg tatgccggtt ctgtggacgc gcggcgtgcg aacgggccgc ctgacaccta atgagttcgt ggccgtcact tccacgaaca tcgcgaaaat cctcaacatc tatccgcaga aaggtgccgt ggtgccaggc gcggacgccg atcttgttat atgggatccg gagacgacca agaagatttc cgccaagacg cagcattcct ccatcgatta caacgtgttc gagggtttcg agctgaaggg gctgccgatc atgacgctgt cgcgcgggcg gatcgccttc gacaagggac aggtgacagc aaaaccgggc gacgggcgct tcatcgagcg tgagccgaat ggcgcggtca atcgcgcgct atcgcaatgg aaggaaattg tcgcgccgcg caaggtggag cgcagtgccg aacatatgcc gataggagtc tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for BrsuA.01123.b.B1.PS01876
| PURIFICATION DATe: | 8/1/2013 |
| CONCENTRATION: | 19.16mg/ml |
| OBSERVED MW: | 63kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 7 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 85 |
Protocol Notes
notes unavailable
Validated AA Sequence
MPGGIDPHTH LQMPFMGTYS SDDFDTGTAA ALAGGTTMVV DFVLPDSEGN LLDALQEWFQ KAGKARTDYS FHMAITGWNE RTFNEMAEVV KRGINTFKHF MAYKGALMVN DDEMFASFQR CAELGAMPLV HAENGDIVAQ LQAKLMAEGN DGPEAHAYSR PPEVEGEATN RAIMIADQAG VPLYVVHVSC EQSHEAIRRA RQKGMRVFGE PLIQHLTLXE SEYHNRDWDY AARRVMSPPF RDKLNQDSLW AGLAAGSLQC VATDHCAFTT EQKRYGIGNF TKIPNGTGGL EERMPVLWTR GVRTGRLTPN EFVAVTSTNI AKILNIYPQK GAVVPGADAD LVIWDPETTK KISAKTQHSS IDYNVFEGFE LKGLPIMTLS RGRIAFDKGQ VTAKPGDGRF IEREPNGAVN RALSQWKEIV APRKVE
Validated NT Sequence
cgctccacct tgcgcggcgc gacaatttcc ttccattgcg atagcgcgcg attgaccgcg ccattcggct cacgctcgat gaagcgcccg tcgcccggtt ttgctgtcac ctgtcccttg tcgaaggcga tccgcccgcg cgacagcgtc atgatcggca gccccttcag ctcgaaaccc tcgaacacgt tgtaatcgat ggaggaatgc tgcgtcttgg cggaaatctt cttggtcgtc tccggatccc atataacaag atcggcgtcc gcgcctggca ccacggcacc tttctgcgga tagatgttga ggattttcgc gatgttcgtg gaagtgacgg ccacgaactc attaggtgtc aggcggcccg ttcgcacgcc gcgcgtccac agaaccggca tacgttcctc cagcccgccg gtgccgttcg gaatcttggt gaaattgccg atgccatagc gtttctgctc ggtggtgaag gcgcaatgat cggtcgccac gcattgcagg cttccagccg caaggcccgc ccagaggctg tcctgattga gcttgtcgcg gaacggcggc gacatcacgc gccgcgcggc atagtcccag tcgcgattgt gatattcgct ttcntcgagc gtcagatgct ggatcagcgg ctcgccgaaa acacgcatac ccttctggcg cgcgcggcgg attgcttcat ggctttgctc gcaggagaca tgcaccacat aaagcggtac gcccgcctga tcggcgatca tgatggcgcg attggtggct tctccttcca cttccggcgg gcgggaatag gcatgggctt ccggcccgtc attgccttcg gccatgagtt tcgcctgcaa ttgcgcgacg atatcgccgt tttccgcatg gacgagcggc atggcaccca gttccgcaca acgctggaac gaggcgaaca tctcatcgtc attcaccatc agcgcgccct tataggccat gaaatgcttg aacgtgttga tgccgcgctt caccacttcg gccatttcgt tgaaagtgcg ctcgttccag ccggtgatcg ccatgtggaa cgaataatcg gtgcgcgcct tgcccgcctt ctgaaaccat tcctgaagcg catccagaag attgccctcg gaatccggca ggacgaaatc gaccaccatc gtcgtgccgc cggcaagtgc tgccgccgtg cccgtgtcga aatcgtcgga ggaatatgtg cccatgaagg gcatttgcag gtgggtgtgc gggtcgatgc cgcccggcat gacatagcag ccggatgcgt cgatgacctc atcgccttca aggctatcgc caatggcggc gatcttgccg tcttcgataa gaatatctgc cctgaacgtg cggtcagccg tgatgacggt gccgcccttg atgactttgc ttgccccggt c
Expressed Protein Sequence
MAHHHHHHMA KTGASKVIKG GTVITADRTF RADILIEDGK IAAIGDSLEG DEVIDASGCY VMPGGIDPHT HLQMPFMGTY SSDDFDTGTA AALAGGTTMV VDFVLPDSEG NLLDALQEWF QKAGKARTDY SFHMAITGWN ERTFNEMAEV VKRGINTFKH FMAYKGALMV NDDEMFASFQ RCAELGAMPL VHAENGDIVA QLQAKLMAEG NDGPEAHAYS RPPEVEGEAT NRAIMIADQA GVPLYVVHVS CEQSHEAIRR ARQKGMRVFG EPLIQHLTLD ESEYHNRDWD YAARRVMSPP FRDKLNQDSL WAGLAAGSLQ CVATDHCAFT TEQKRYGIGN FTKIPNGTGG LEERMPVLWT RGVRTGRLTP NEFVAVTSTN IAKILNIYPQ KGAVVPGADA DLVIWDPETT KKISAKTQHS SIDYNVFEGF ELKGLPIMTL SRGRIAFDKG QVTAKPGDGR FIEREPNGAV NRALSQWKEI VAPRKVERSA EHMPIGV
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCCA AGACCGGGGC AAGCAAAGTC ATCAAGGGCG GCACCGTCAT CACGGCTGAC CGCACGTTCA GGGCAGATAT TCTTATCGAA GACGGCAAGA TCGCCGCCAT TGGCGATAGC CTTGAAGGCG ATGAGGTCAT CGACGCATCC GGCTGCTATG TCATGCCGGG CGGCATCGAC CCGCACACCC ACCTGCAAAT GCCCTTCATG GGCACATATT CCTCCGACGA TTTCGACACG GGCACGGCGG CAGCACTTGC CGGCGGCACG ACGATGGTGG TCGATTTCGT CCTGCCGGAT TCCGAGGGCA ATCTTCTGGA TGCGCTTCAG GAATGGTTTC AGAAGGCGGG CAAGGCGCGC ACCGATTATT CGTTCCACAT GGCGATCACC GGCTGGAACG AGCGCACTTT CAACGAAATG GCCGAAGTGG TGAAGCGCGG CATCAACACG TTCAAGCATT TCATGGCCTA TAAGGGCGCG CTGATGGTGA ATGACGATGA GATGTTCGCC TCGTTCCAGC GTTGTGCGGA ACTGGGTGCC ATGCCGCTCG TCCATGCGGA AAACGGCGAT ATCGTCGCGC AATTGCAGGC GAAACTCATG GCCGAAGGCA ATGACGGGCC GGAAGCCCAT GCCTATTCCC GCCCGCCGGA AGTGGAAGGA GAAGCCACCA ATCGCGCCAT CATGATCGCC GATCAGGCGG GCGTACCGCT TTATGTGGTG CATGTCTCCT GCGAGCAAAG CCATGAAGCA ATCCGCCGCG CGCGCCAGAA GGGTATGCGT GTTTTCGGCG AGCCGCTGAT CCAGCATCTG ACGCTCGATG AAAGCGAATA TCACAATCGC GACTGGGACT ATGCCGCGCG GCGCGTGATG TCGCCGCCGT TCCGCGACAA GCTCAATCAG GACAGCCTCT GGGCGGGCCT TGCGGCTGGA AGCCTGCAAT GCGTGGCGAC CGATCATTGC GCCTTCACCA CCGAGCAGAA ACGCTATGGC ATCGGCAATT TCACCAAGAT TCCGAACGGC ACCGGCGGGC TGGAGGAACG TATGCCGGTT CTGTGGACGC GCGGCGTGCG AACGGGCCGC CTGACACCTA ATGAGTTCGT GGCCGTCACT TCCACGAACA TCGCGAAAAT CCTCAACATC TATCCGCAGA AAGGTGCCGT GGTGCCAGGC GCGGACGCCG ATCTTGTTAT ATGGGATCCG GAGACGACCA AGAAGATTTC CGCCAAGACG CAGCATTCCT CCATCGATTA CAACGTGTTC GAGGGTTTCG AGCTGAAGGG GCTGCCGATC ATGACGCTGT CGCGCGGGCG GATCGCCTTC GACAAGGGAC AGGTGACAGC AAAACCGGGC GACGGGCGCT TCATCGAGCG TGAGCCGAAT GGCGCGGTCA ATCGCGCGCT ATCGCAATGG AAGGAAATTG TCGCGCCGCG CAAGGTGGAG CGCAGTGCCG AACATATGCC GATAGGAGTC TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT