BrsuA.01123.b
Dihydropyrimidinase (EC 3.5.2.2)
CENTER ID:
BrsuA.01123.b
ORGANISM:
Brucella suis 1330
ASSOCIATED DISEASE:
Brucellosis
CURRENT STATUS:
in PDB
COMMUNITY REQUEST:
True
NIH RISK GROUP:
3
SELECT AGENT:
True
NIH PRIORITY
pathogens category:
IIIB
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO
AA
START
AA
STOP
ORDER
MATERIAL
BrsuA.01123.b.B1.GE36774
Full length( BrsuA.01123.b )
info
1
489
BrsuA.01123.b.B1.GE36774
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis . Get sequence information
using the button in the "info" column.
Proteins
CENTER REFERENCE ID
DOMAIN/REGION DESCRIPTION
INFO
AA START
AA STOP
ORDER MATERIAL
BrsuA.01123.b.B1.PS01876
Full length( BrsuA.01123.b )
info
1
489
PS01876
Structures
4TQT
DEPOSITED: 6/12/2014
DETERMINATION: XRay
CLONE: BrsuA.01123.b.B1.GE36774
PROTEIN: BrsuA.01123.b.B1.PS01876
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAKTGASKVI KGGTVITADR TFRADILIED GKIAAIGDSL EGDEVIDASG CYVMPGGIDP HTHLQMPFMG TYSSDDFDTG TAAALAGGTT MVVDFVLPDS EGNLLDALQE WFQKAGKART DYSFHMAITG WNERTFNEMA EVVKRGINTF KHFMAYKGAL MVNDDEMFAS FQRCAELGAM PLVHAENGDI VAQLQAKLMA EGNDGPEAHA YSRPPEVEGE ATNRAIMIAD QAGVPLYVVH VSCEQSHEAI RRARQKGMRV FGEPLIQHLT LDESEYHNRD WDYAARRVMS PPFRDKLNQD SLWAGLAAGS LQCVATDHCA FTTEQKRYGI GNFTKIPNGT GGLEERMPVL WTRGVRTGRL TPNEFVAVTS TNIAKILNIY PQKGAVVPGA DADLVIWDPE TTKKISAKTQ HSSIDYNVFE GFELKGLPIM TLSRGRIAFD KGQVTAKPGD GRFIEREPNG AVNRALSQWK EIVAPRKVER SAEHMPIGV
NT Sequence
ATGGCCAAGA CCGGGGCAAG CAAAGTCATC AAGGGCGGCA CCGTCATCAC GGCTGACCGC ACGTTCAGGG CAGATATTCT TATCGAAGAC GGCAAGATCG CCGCCATTGG CGATAGCCTT GAAGGCGATG AGGTCATCGA CGCATCCGGC TGCTATGTCA TGCCGGGCGG CATCGACCCG CACACCCACC TGCAAATGCC CTTCATGGGC ACATATTCCT CCGACGATTT CGACACGGGC ACGGCGGCAG CACTTGCCGG CGGCACGACG ATGGTGGTCG ATTTCGTCCT GCCGGATTCC GAGGGCAATC TTCTGGATGC GCTTCAGGAA TGGTTTCAGA AGGCGGGCAA GGCGCGCACC GATTATTCGT TCCACATGGC GATCACCGGC TGGAACGAGC GCACTTTCAA CGAAATGGCC GAAGTGGTGA AGCGCGGCAT CAACACGTTC AAGCATTTCA TGGCCTATAA GGGCGCGCTG ATGGTGAATG ACGATGAGAT GTTCGCCTCG TTCCAGCGTT GTGCGGAACT GGGTGCCATG CCGCTCGTCC ATGCGGAAAA CGGCGATATC GTCGCGCAAT TGCAGGCGAA ACTCATGGCC GAAGGCAATG ACGGGCCGGA AGCCCATGCC TATTCCCGCC CGCCGGAAGT GGAAGGAGAA GCCACCAATC GCGCCATCAT GATCGCCGAT CAGGCGGGCG TACCGCTTTA TGTGGTGCAT GTCTCCTGCG AGCAAAGCCA TGAAGCAATC CGCCGCGCGC GCCAGAAGGG TATGCGTGTT TTCGGCGAGC CGCTGATCCA GCATCTGACG CTCGATGAAA GCGAATATCA CAATCGCGAC TGGGACTATG CCGCGCGGCG CGTGATGTCG CCGCCGTTCC GCGACAAGCT CAATCAGGAC AGCCTCTGGG CGGGCCTTGC GGCTGGAAGC CTGCAATGCG TGGCGACCGA TCATTGCGCC TTCACCACCG AGCAGAAACG CTATGGCATC GGCAATTTCA CCAAGATTCC GAACGGCACC GGCGGGCTGG AGGAACGTAT GCCGGTTCTG TGGACGCGCG GCGTGCGAAC GGGCCGCCTG ACACCTAATG AGTTCGTGGC CGTCACTTCC ACGAACATCG CGAAAATCCT CAACATCTAT CCGCAGAAAG GTGCCGTGGT GCCAGGCGCG GACGCCGATC TTGTTATATG GGATCCGGAG ACGACCAAGA AGATTTCCGC CAAGACGCAG CATTCCTCCA TCGATTACAA CGTGTTCGAG GGTTTCGAGC TGAAGGGGCT GCCGATCATG ACGCTGTCGC GCGGGCGGAT CGCCTTCGAC AAGGGACAGG TGACAGCAAA ACCGGGCGAC GGGCGCTTCA TCGAGCGTGA GCCGAATGGC GCGGTCAATC GCGCGCTATC GCAATGGAAG GAAATTGTCG CGCCGCGCAA GGTGGAGCGC AGTGCCGAAC ATATGCCGAT AGGAGTC
Details for BrsuA.01123.b.B1.GE36774
HARVESTED ON:
3/27/2013
SEQUENCED ON:
3/30/2013
EXPECTED MW:
55kDa
OBSERVED MW:
55kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Too many to count (100+)
TOTAL EXPRESSION LEVEL:
High Expression
SOLUBLE EXPRESSION LEVEL
High Expression
EXPRESSION HOST:
data unavailable
SEQUENCING RESULT:
pass with incomplete coverage
PERCENT IDENTITY:
99
PERCENT COVERAGE:
85
Details for BrsuA.01123.b.B1.PS01876
PURIFICATION DATe:
8/1/2013
CONCENTRATION:
19.16mg/ml
OBSERVED MW:
63kDa
EXPRESSION LEVEL:
Moderate Expression
PROTEIN PURIFICATION BUFFER:
20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:
data unavailable
VIAL COUNT (approx.):
7
VIAL VOLUME:
200µl
PERCENT IDENTITY:
99
PERCENT COVERAGE:
85