BrsuA.01104.a
Cysteine desulfurase (EC 2.8.1.7), SufS subfamily
| CENTER ID: | BrsuA.01104.a |
| ORGANISM: | Brucella suis 1330 |
| ASSOCIATED DISEASE: | Brucellosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrsuA.01104.a.B1.GE36773 | Full length( BrsuA.01104.a ) | 1 | 414 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrsuA.01104.a.B1.PS01849 | Full length( BrsuA.01104.a ) | 1 | 414 | ||
| BrsuA.01104.a.B1.PS01850 | Full length( BrsuA.01104.a ) | 1 | 414 |
Structures
4W91
DEPOSITED: 8/26/2014
DETERMINATION: XRay
CLONE: BrsuA.01104.a.B1.GE36773
PROTEIN: BrsuA.01104.a.B1.PS01850
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | PATRIC_Locus_Tag:VBIBruSui107850_0949 |
| BV-BRC: | fig|204722.5.peg.949 |
| RefSeq: | NP_697945.1 |
| RefSeq: | YP_005615769.1 |
| UniProt: | A0A0H3GBE5 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDQKNPLTMA YDVEAIRRDF PILSRQVHGK TLVYLDNGAS AQKPQSVIDA VTHAYANEYA NVHRGLHFLS NAATDAYEKS RETVRRFLNA GSVDEIVFTK NATEAINTVA YGYGMPFIGE GDEILLSIME HHSNIVPWHF IRERQGAKLV FTPVDDNGVF HIEEFEKRLS ERTKLVAITH MSNTLGTVVP IKKIVELAHA RGIPVLVDGS QGAVHLPVDV QDLGCDWYVF TGHKVYGPSG IGVLYGRAQM LEKMRPFQGG GEMIEEVTEE NVTYNHPPHR FEAGTPPIVQ AIGLGAALEY MEKIGRHAIL AHEADLRDYA HERLGRINSL RIFGNAPDKG AIISFALEGI HAHDVSMVID RAGVAVRAGT HCAQPLLKRF GVTSTCRASF ALYNTRAEVD ALAEALEKAR KFFG
NT Sequence
ATGGATCAGA AGAATCCGCT CACCATGGCC TATGATGTCG AGGCCATACG CAGGGATTTC CCGATTTTGT CGCGGCAGGT GCACGGCAAA ACGCTCGTTT ATCTGGATAA CGGCGCTTCC GCACAGAAGC CGCAAAGCGT TATCGACGCG GTAACTCATG CTTACGCGAA TGAATATGCC AATGTTCATC GCGGTTTGCA TTTTCTCTCG AACGCTGCCA CCGACGCTTA TGAGAAGTCG CGTGAAACCG TGCGACGTTT TCTCAATGCC GGTTCTGTGG ACGAGATCGT CTTCACCAAG AATGCGACAG AGGCGATCAA TACCGTCGCT TATGGCTATG GAATGCCGTT CATCGGCGAG GGCGATGAAA TCCTGCTCTC GATCATGGAG CATCATTCCA ACATCGTGCC GTGGCATTTC ATACGCGAAC GCCAGGGCGC GAAACTGGTT TTCACACCAG TCGACGATAA CGGTGTTTTC CACATCGAAG AGTTCGAGAA GCGCCTGAGC GAACGTACCA AACTCGTTGC CATCACGCAT ATGTCGAATA CACTCGGCAC GGTGGTCCCG ATCAAGAAGA TCGTGGAGCT TGCCCATGCA CGCGGCATTC CGGTTCTGGT CGATGGCAGT CAGGGTGCAG TGCACCTGCC GGTCGATGTG CAGGATCTGG GCTGTGACTG GTATGTCTTT ACCGGCCACA AAGTCTATGG CCCTTCGGGC ATCGGCGTGC TTTATGGACG CGCGCAAATG CTGGAAAAGA TGCGCCCGTT CCAGGGCGGG GGCGAAATGA TCGAGGAAGT GACGGAGGAA AACGTCACCT ACAATCATCC GCCACACCGC TTTGAAGCAG GCACGCCGCC TATCGTTCAG GCGATTGGTC TGGGTGCGGC GCTTGAATAT ATGGAAAAGA TCGGGCGTCA TGCGATCCTG GCTCACGAGG CAGACTTGCG TGATTATGCG CATGAACGGC TCGGCCGGAT CAACAGCTTG CGCATTTTCG GCAATGCGCC GGACAAGGGC GCAATCATCT CCTTCGCGCT TGAAGGCATT CATGCCCATG ACGTGTCGAT GGTGATCGAT CGGGCGGGGG TCGCGGTGCG CGCGGGAACG CATTGTGCGC AACCGCTCTT GAAACGCTTT GGCGTCACCT CTACATGCCG TGCATCCTTT GCCCTTTATA ATACCCGCGC CGAAGTAGAC GCATTGGCTG AAGCCTTGGA AAAGGCAAGG AAGTTTTTCG GA
Details for BrsuA.01104.a.B1.GE36773
| HARVESTED ON: | 3/27/2013 |
| SEQUENCED ON: | 3/30/2013 |
| EXPECTED MW: | 47kDa |
| OBSERVED MW: | 47kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 94 |
Validated AA Sequence
MAYDVEAIRR DFPILSRQVH GKTLVYLDNG ASAQKPQSVI DAVTHAYANE YANVHRGLHF LSNAATDAYE KSRETVRRFL NAGSVDEIVF TKNATEAINT VAYGYGMPFI GEGDEILLSI MEHHSNIVPW HFIRERQGAK LVFTPVDDNG VFHIEEFEKR LSERTKLVAI THMSNTLGTV VPIKKIVELA HARGIPVLVD GSQGAVHLPV DVQDLGCDWY VFTGHKVYGP SGIGVLYGRA QMLEKMRPFQ GGGEMIEEVT EENVTYNHPP HRFEAGTPPI VQAIGXGAAL EYMEKIGRHA ILAHEADLRD YAHERLGRIN SLRIFGNAPD KGAIISFALE GIHAHDVSMV IDRAGVAVRA GTHCAQPLLK RFGVTSTCRA SFALYNTRAE VDALAEA
Validated NT Sequence
caaggcttca gccaatgcgt ctacttcggc gcgggtatta taaagggcaa aggatgcacg gcatgtagag gtgacgccaa agcgtttcaa gagcggttgc gcacaatgcg ttcccgcgcg caccgcgacc cccgcccgat cgatcaccat cgacacgtca tgggcatgaa tgccttcaag cgcgaaggag atgattgcgc ccttgtccgg cgcattgccg aaaatgcgca agctgttgat ccggccgagc cgttcatgcg cataatcacg caagtctgcc tcgtgagcca ggatcgcatg acgcccgatc ttttccatat attcaagcgc cgcacccana ccaatcgcct gaacgatagg cggcgtgcct gcttcaaagc ggtgtggcgg atgattgtag gtgacgtttt cctccgtcac ttcctcgatc atttcgcccc cgccctggaa cgggcgcatc ttttccagca tttgcgcgcg tccataaagc acgccgatgc ccgaagggcc atagactttg tggccggtaa agacatacca gtcacagccc agatcctgca catcgaccgg caggtgcact gcaccctgac tgccatcgac cagaaccgga atgccgcgtg catgggcaag ctccacgatc ttcttgatcg ggaccaccgt gccgagtgta ttcgacatat gcgtgatggc aacgagtttg gtacgttcgc tcaggcgctt ctcgaactct tcgatgtgga aaacaccgtt atcgtcgact ggtgtgaaaa ccagtttcgc gccctggcgt tcgcgtatga aatgccacgg cacgatgttg gaatgatgct ccatgatcga gagcaggatt tcatcgccct cgccgatgaa cggcattcca tagccataag cgacggtatt gatcgcctct gtcgcattct tggtgaagac gatctcgtcc acagaaccgg cattgagaaa acgtcgcacg gtttcacgcg acttctcata agcgtcggtg gcagcgttcg agagaaaatg caaaccgcga tgaacattgg catattcatt cgcgtaagca tgagttaccg cgtcgataac gctttgcggc ttctgtgcgg aagcgccgtt atccagataa acgagcgttt tgccgtgcac ctgccgcgac aaaatcggga aatccctgcg tatggcctcg acatcatagg ccatggtgag cggattcttc t
Expected Protein Sequence
MAHHHHHHMD QKNPLTMAYD VEAIRRDFPI LSRQVHGKTL VYLDNGASAQ KPQSVIDAVT HAYANEYANV HRGLHFLSNA ATDAYEKSRE TVRRFLNAGS VDEIVFTKNA TEAINTVAYG YGMPFIGEGD EILLSIMEHH SNIVPWHFIR ERQGAKLVFT PVDDNGVFHI EEFEKRLSER TKLVAITHMS NTLGTVVPIK KIVELAHARG IPVLVDGSQG AVHLPVDVQD LGCDWYVFTG HKVYGPSGIG VLYGRAQMLE KMRPFQGGGE MIEEVTEENV TYNHPPHRFE AGTPPIVQAI GLGAALEYME KIGRHAILAH EADLRDYAHE RLGRINSLRI FGNAPDKGAI ISFALEGIHA HDVSMVIDRA GVAVRAGTHC AQPLLKRFGV TSTCRASFAL YNTRAEVDAL AEALEKARKF FG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggatc agaagaatcc gctcaccatg gcctatgatg tcgaggccat acgcagggat ttcccgattt tgtcgcggca ggtgcacggc aaaacgctcg tttatctgga taacggcgct tccgcacaga agccgcaaag cgttatcgac gcggtaactc atgcttacgc gaatgaatat gccaatgttc atcgcggttt gcattttctc tcgaacgctg ccaccgacgc ttatgagaag tcgcgtgaaa ccgtgcgacg ttttctcaat gccggttctg tggacgagat cgtcttcacc aagaatgcga cagaggcgat caataccgtc gcttatggct atggaatgcc gttcatcggc gagggcgatg aaatcctgct ctcgatcatg gagcatcatt ccaacatcgt gccgtggcat ttcatacgcg aacgccaggg cgcgaaactg gttttcacac cagtcgacga taacggtgtt ttccacatcg aagagttcga gaagcgcctg agcgaacgta ccaaactcgt tgccatcacg catatgtcga atacactcgg cacggtggtc ccgatcaaga agatcgtgga gcttgcccat gcacgcggca ttccggttct ggtcgatggc agtcagggtg cagtgcacct gccggtcgat gtgcaggatc tgggctgtga ctggtatgtc tttaccggcc acaaagtcta tggcccttcg ggcatcggcg tgctttatgg acgcgcgcaa atgctggaaa agatgcgccc gttccagggc gggggcgaaa tgatcgagga agtgacggag gaaaacgtca cctacaatca tccgccacac cgctttgaag caggcacgcc gcctatcgtt caggcgattg gtctgggtgc ggcgcttgaa tatatggaaa agatcgggcg tcatgcgatc ctggctcacg aggcagactt gcgtgattat gcgcatgaac ggctcggccg gatcaacagc ttgcgcattt tcggcaatgc gccggacaag ggcgcaatca tctccttcgc gcttgaaggc attcatgccc atgacgtgtc gatggtgatc gatcgggcgg gggtcgcggt gcgcgcggga acgcattgtg cgcaaccgct cttgaaacgc tttggcgtca cctctacatg ccgtgcatcc tttgcccttt ataatacccg cgccgaagta gacgcattgg ctgaagcctt ggaaaaggca aggaagtttt tcggatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for BrsuA.01104.a.B1.PS01849
| PURIFICATION DATe: | 6/27/2013 |
| CONCENTRATION: | 67.4mg/ml |
| OBSERVED MW: | 45kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 5 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 94 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAYDVEAIRR DFPILSRQVH GKTLVYLDNG ASAQKPQSVI DAVTHAYANE YANVHRGLHF LSNAATDAYE KSRETVRRFL NAGSVDEIVF TKNATEAINT VAYGYGMPFI GEGDEILLSI MEHHSNIVPW HFIRERQGAK LVFTPVDDNG VFHIEEFEKR LSERTKLVAI THMSNTLGTV VPIKKIVELA HARGIPVLVD GSQGAVHLPV DVQDLGCDWY VFTGHKVYGP SGIGVLYGRA QMLEKMRPFQ GGGEMIEEVT EENVTYNHPP HRFEAGTPPI VQAIGXGAAL EYMEKIGRHA ILAHEADLRD YAHERLGRIN SLRIFGNAPD KGAIISFALE GIHAHDVSMV IDRAGVAVRA GTHCAQPLLK RFGVTSTCRA SFALYNTRAE VDALAEA
Validated NT Sequence
caaggcttca gccaatgcgt ctacttcggc gcgggtatta taaagggcaa aggatgcacg gcatgtagag gtgacgccaa agcgtttcaa gagcggttgc gcacaatgcg ttcccgcgcg caccgcgacc cccgcccgat cgatcaccat cgacacgtca tgggcatgaa tgccttcaag cgcgaaggag atgattgcgc ccttgtccgg cgcattgccg aaaatgcgca agctgttgat ccggccgagc cgttcatgcg cataatcacg caagtctgcc tcgtgagcca ggatcgcatg acgcccgatc ttttccatat attcaagcgc cgcacccana ccaatcgcct gaacgatagg cggcgtgcct gcttcaaagc ggtgtggcgg atgattgtag gtgacgtttt cctccgtcac ttcctcgatc atttcgcccc cgccctggaa cgggcgcatc ttttccagca tttgcgcgcg tccataaagc acgccgatgc ccgaagggcc atagactttg tggccggtaa agacatacca gtcacagccc agatcctgca catcgaccgg caggtgcact gcaccctgac tgccatcgac cagaaccgga atgccgcgtg catgggcaag ctccacgatc ttcttgatcg ggaccaccgt gccgagtgta ttcgacatat gcgtgatggc aacgagtttg gtacgttcgc tcaggcgctt ctcgaactct tcgatgtgga aaacaccgtt atcgtcgact ggtgtgaaaa ccagtttcgc gccctggcgt tcgcgtatga aatgccacgg cacgatgttg gaatgatgct ccatgatcga gagcaggatt tcatcgccct cgccgatgaa cggcattcca tagccataag cgacggtatt gatcgcctct gtcgcattct tggtgaagac gatctcgtcc acagaaccgg cattgagaaa acgtcgcacg gtttcacgcg acttctcata agcgtcggtg gcagcgttcg agagaaaatg caaaccgcga tgaacattgg catattcatt cgcgtaagca tgagttaccg cgtcgataac gctttgcggc ttctgtgcgg aagcgccgtt atccagataa acgagcgttt tgccgtgcac ctgccgcgac aaaatcggga aatccctgcg tatggcctcg acatcatagg ccatggtgag cggattcttc t
Expressed Protein Sequence
MAHHHHHHMD QKNPLTMAYD VEAIRRDFPI LSRQVHGKTL VYLDNGASAQ KPQSVIDAVT HAYANEYANV HRGLHFLSNA ATDAYEKSRE TVRRFLNAGS VDEIVFTKNA TEAINTVAYG YGMPFIGEGD EILLSIMEHH SNIVPWHFIR ERQGAKLVFT PVDDNGVFHI EEFEKRLSER TKLVAITHMS NTLGTVVPIK KIVELAHARG IPVLVDGSQG AVHLPVDVQD LGCDWYVFTG HKVYGPSGIG VLYGRAQMLE KMRPFQGGGE MIEEVTEENV TYNHPPHRFE AGTPPIVQAI GLGAALEYME KIGRHAILAH EADLRDYAHE RLGRINSLRI FGNAPDKGAI ISFALEGIHA HDVSMVIDRA GVAVRAGTHC AQPLLKRFGV TSTCRASFAL YNTRAEVDAL AEALEKARKF FG
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGATC AGAAGAATCC GCTCACCATG GCCTATGATG TCGAGGCCAT ACGCAGGGAT TTCCCGATTT TGTCGCGGCA GGTGCACGGC AAAACGCTCG TTTATCTGGA TAACGGCGCT TCCGCACAGA AGCCGCAAAG CGTTATCGAC GCGGTAACTC ATGCTTACGC GAATGAATAT GCCAATGTTC ATCGCGGTTT GCATTTTCTC TCGAACGCTG CCACCGACGC TTATGAGAAG TCGCGTGAAA CCGTGCGACG TTTTCTCAAT GCCGGTTCTG TGGACGAGAT CGTCTTCACC AAGAATGCGA CAGAGGCGAT CAATACCGTC GCTTATGGCT ATGGAATGCC GTTCATCGGC GAGGGCGATG AAATCCTGCT CTCGATCATG GAGCATCATT CCAACATCGT GCCGTGGCAT TTCATACGCG AACGCCAGGG CGCGAAACTG GTTTTCACAC CAGTCGACGA TAACGGTGTT TTCCACATCG AAGAGTTCGA GAAGCGCCTG AGCGAACGTA CCAAACTCGT TGCCATCACG CATATGTCGA ATACACTCGG CACGGTGGTC CCGATCAAGA AGATCGTGGA GCTTGCCCAT GCACGCGGCA TTCCGGTTCT GGTCGATGGC AGTCAGGGTG CAGTGCACCT GCCGGTCGAT GTGCAGGATC TGGGCTGTGA CTGGTATGTC TTTACCGGCC ACAAAGTCTA TGGCCCTTCG GGCATCGGCG TGCTTTATGG ACGCGCGCAA ATGCTGGAAA AGATGCGCCC GTTCCAGGGC GGGGGCGAAA TGATCGAGGA AGTGACGGAG GAAAACGTCA CCTACAATCA TCCGCCACAC CGCTTTGAAG CAGGCACGCC GCCTATCGTT CAGGCGATTG GTCTGGGTGC GGCGCTTGAA TATATGGAAA AGATCGGGCG TCATGCGATC CTGGCTCACG AGGCAGACTT GCGTGATTAT GCGCATGAAC GGCTCGGCCG GATCAACAGC TTGCGCATTT TCGGCAATGC GCCGGACAAG GGCGCAATCA TCTCCTTCGC GCTTGAAGGC ATTCATGCCC ATGACGTGTC GATGGTGATC GATCGGGCGG GGGTCGCGGT GCGCGCGGGA ACGCATTGTG CGCAACCGCT CTTGAAACGC TTTGGCGTCA CCTCTACATG CCGTGCATCC TTTGCCCTTT ATAATACCCG CGCCGAAGTA GACGCATTGG CTGAAGCCTT GGAAAAGGCA AGGAAGTTTT TCGGATGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT
Details for BrsuA.01104.a.B1.PS01850
| PURIFICATION DATe: | 6/27/2013 |
| CONCENTRATION: | 50.2mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 5 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 94 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAYDVEAIRR DFPILSRQVH GKTLVYLDNG ASAQKPQSVI DAVTHAYANE YANVHRGLHF LSNAATDAYE KSRETVRRFL NAGSVDEIVF TKNATEAINT VAYGYGMPFI GEGDEILLSI MEHHSNIVPW HFIRERQGAK LVFTPVDDNG VFHIEEFEKR LSERTKLVAI THMSNTLGTV VPIKKIVELA HARGIPVLVD GSQGAVHLPV DVQDLGCDWY VFTGHKVYGP SGIGVLYGRA QMLEKMRPFQ GGGEMIEEVT EENVTYNHPP HRFEAGTPPI VQAIGXGAAL EYMEKIGRHA ILAHEADLRD YAHERLGRIN SLRIFGNAPD KGAIISFALE GIHAHDVSMV IDRAGVAVRA GTHCAQPLLK RFGVTSTCRA SFALYNTRAE VDALAEA
Validated NT Sequence
caaggcttca gccaatgcgt ctacttcggc gcgggtatta taaagggcaa aggatgcacg gcatgtagag gtgacgccaa agcgtttcaa gagcggttgc gcacaatgcg ttcccgcgcg caccgcgacc cccgcccgat cgatcaccat cgacacgtca tgggcatgaa tgccttcaag cgcgaaggag atgattgcgc ccttgtccgg cgcattgccg aaaatgcgca agctgttgat ccggccgagc cgttcatgcg cataatcacg caagtctgcc tcgtgagcca ggatcgcatg acgcccgatc ttttccatat attcaagcgc cgcacccana ccaatcgcct gaacgatagg cggcgtgcct gcttcaaagc ggtgtggcgg atgattgtag gtgacgtttt cctccgtcac ttcctcgatc atttcgcccc cgccctggaa cgggcgcatc ttttccagca tttgcgcgcg tccataaagc acgccgatgc ccgaagggcc atagactttg tggccggtaa agacatacca gtcacagccc agatcctgca catcgaccgg caggtgcact gcaccctgac tgccatcgac cagaaccgga atgccgcgtg catgggcaag ctccacgatc ttcttgatcg ggaccaccgt gccgagtgta ttcgacatat gcgtgatggc aacgagtttg gtacgttcgc tcaggcgctt ctcgaactct tcgatgtgga aaacaccgtt atcgtcgact ggtgtgaaaa ccagtttcgc gccctggcgt tcgcgtatga aatgccacgg cacgatgttg gaatgatgct ccatgatcga gagcaggatt tcatcgccct cgccgatgaa cggcattcca tagccataag cgacggtatt gatcgcctct gtcgcattct tggtgaagac gatctcgtcc acagaaccgg cattgagaaa acgtcgcacg gtttcacgcg acttctcata agcgtcggtg gcagcgttcg agagaaaatg caaaccgcga tgaacattgg catattcatt cgcgtaagca tgagttaccg cgtcgataac gctttgcggc ttctgtgcgg aagcgccgtt atccagataa acgagcgttt tgccgtgcac ctgccgcgac aaaatcggga aatccctgcg tatggcctcg acatcatagg ccatggtgag cggattcttc t
Expressed Protein Sequence
MAHHHHHHMD QKNPLTMAYD VEAIRRDFPI LSRQVHGKTL VYLDNGASAQ KPQSVIDAVT HAYANEYANV HRGLHFLSNA ATDAYEKSRE TVRRFLNAGS VDEIVFTKNA TEAINTVAYG YGMPFIGEGD EILLSIMEHH SNIVPWHFIR ERQGAKLVFT PVDDNGVFHI EEFEKRLSER TKLVAITHMS NTLGTVVPIK KIVELAHARG IPVLVDGSQG AVHLPVDVQD LGCDWYVFTG HKVYGPSGIG VLYGRAQMLE KMRPFQGGGE MIEEVTEENV TYNHPPHRFE AGTPPIVQAI GLGAALEYME KIGRHAILAH EADLRDYAHE RLGRINSLRI FGNAPDKGAI ISFALEGIHA HDVSMVIDRA GVAVRAGTHC AQPLLKRFGV TSTCRASFAL YNTRAEVDAL AEALEKARKF FG
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGATC AGAAGAATCC GCTCACCATG GCCTATGATG TCGAGGCCAT ACGCAGGGAT TTCCCGATTT TGTCGCGGCA GGTGCACGGC AAAACGCTCG TTTATCTGGA TAACGGCGCT TCCGCACAGA AGCCGCAAAG CGTTATCGAC GCGGTAACTC ATGCTTACGC GAATGAATAT GCCAATGTTC ATCGCGGTTT GCATTTTCTC TCGAACGCTG CCACCGACGC TTATGAGAAG TCGCGTGAAA CCGTGCGACG TTTTCTCAAT GCCGGTTCTG TGGACGAGAT CGTCTTCACC AAGAATGCGA CAGAGGCGAT CAATACCGTC GCTTATGGCT ATGGAATGCC GTTCATCGGC GAGGGCGATG AAATCCTGCT CTCGATCATG GAGCATCATT CCAACATCGT GCCGTGGCAT TTCATACGCG AACGCCAGGG CGCGAAACTG GTTTTCACAC CAGTCGACGA TAACGGTGTT TTCCACATCG AAGAGTTCGA GAAGCGCCTG AGCGAACGTA CCAAACTCGT TGCCATCACG CATATGTCGA ATACACTCGG CACGGTGGTC CCGATCAAGA AGATCGTGGA GCTTGCCCAT GCACGCGGCA TTCCGGTTCT GGTCGATGGC AGTCAGGGTG CAGTGCACCT GCCGGTCGAT GTGCAGGATC TGGGCTGTGA CTGGTATGTC TTTACCGGCC ACAAAGTCTA TGGCCCTTCG GGCATCGGCG TGCTTTATGG ACGCGCGCAA ATGCTGGAAA AGATGCGCCC GTTCCAGGGC GGGGGCGAAA TGATCGAGGA AGTGACGGAG GAAAACGTCA CCTACAATCA TCCGCCACAC CGCTTTGAAG CAGGCACGCC GCCTATCGTT CAGGCGATTG GTCTGGGTGC GGCGCTTGAA TATATGGAAA AGATCGGGCG TCATGCGATC CTGGCTCACG AGGCAGACTT GCGTGATTAT GCGCATGAAC GGCTCGGCCG GATCAACAGC TTGCGCATTT TCGGCAATGC GCCGGACAAG GGCGCAATCA TCTCCTTCGC GCTTGAAGGC ATTCATGCCC ATGACGTGTC GATGGTGATC GATCGGGCGG GGGTCGCGGT GCGCGCGGGA ACGCATTGTG CGCAACCGCT CTTGAAACGC TTTGGCGTCA CCTCTACATG CCGTGCATCC TTTGCCCTTT ATAATACCCG CGCCGAAGTA GACGCATTGG CTGAAGCCTT GGAAAAGGCA AGGAAGTTTT TCGGATGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT