BrsuA.01104.a
Cysteine desulfurase (EC 2.8.1.7), SufS subfamily
CENTER ID:
BrsuA.01104.a
ORGANISM:
Brucella suis 1330
ASSOCIATED DISEASE:
Brucellosis
CURRENT STATUS:
in PDB
COMMUNITY REQUEST:
True
NIH RISK GROUP:
3
SELECT AGENT:
True
NIH PRIORITY
pathogens category:
IIIB
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO
AA
START
AA
STOP
ORDER
MATERIAL
BrsuA.01104.a.B1.GE36773
Full length( BrsuA.01104.a )
info
1
414
BrsuA.01104.a.B1.GE36773
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis . Get sequence information
using the button in the "info" column.
Proteins
CENTER REFERENCE ID
DOMAIN/REGION DESCRIPTION
INFO
AA START
AA STOP
ORDER MATERIAL
BrsuA.01104.a.B1.PS01849
Full length( BrsuA.01104.a )
info
1
414
PS01849
BrsuA.01104.a.B1.PS01850
Full length( BrsuA.01104.a )
info
1
414
PS01850
Structures
4W91
DEPOSITED: 8/26/2014
DETERMINATION: XRay
CLONE: BrsuA.01104.a.B1.GE36773
PROTEIN: BrsuA.01104.a.B1.PS01850
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDQKNPLTMA YDVEAIRRDF PILSRQVHGK TLVYLDNGAS AQKPQSVIDA VTHAYANEYA NVHRGLHFLS NAATDAYEKS RETVRRFLNA GSVDEIVFTK NATEAINTVA YGYGMPFIGE GDEILLSIME HHSNIVPWHF IRERQGAKLV FTPVDDNGVF HIEEFEKRLS ERTKLVAITH MSNTLGTVVP IKKIVELAHA RGIPVLVDGS QGAVHLPVDV QDLGCDWYVF TGHKVYGPSG IGVLYGRAQM LEKMRPFQGG GEMIEEVTEE NVTYNHPPHR FEAGTPPIVQ AIGLGAALEY MEKIGRHAIL AHEADLRDYA HERLGRINSL RIFGNAPDKG AIISFALEGI HAHDVSMVID RAGVAVRAGT HCAQPLLKRF GVTSTCRASF ALYNTRAEVD ALAEALEKAR KFFG
NT Sequence
ATGGATCAGA AGAATCCGCT CACCATGGCC TATGATGTCG AGGCCATACG CAGGGATTTC CCGATTTTGT CGCGGCAGGT GCACGGCAAA ACGCTCGTTT ATCTGGATAA CGGCGCTTCC GCACAGAAGC CGCAAAGCGT TATCGACGCG GTAACTCATG CTTACGCGAA TGAATATGCC AATGTTCATC GCGGTTTGCA TTTTCTCTCG AACGCTGCCA CCGACGCTTA TGAGAAGTCG CGTGAAACCG TGCGACGTTT TCTCAATGCC GGTTCTGTGG ACGAGATCGT CTTCACCAAG AATGCGACAG AGGCGATCAA TACCGTCGCT TATGGCTATG GAATGCCGTT CATCGGCGAG GGCGATGAAA TCCTGCTCTC GATCATGGAG CATCATTCCA ACATCGTGCC GTGGCATTTC ATACGCGAAC GCCAGGGCGC GAAACTGGTT TTCACACCAG TCGACGATAA CGGTGTTTTC CACATCGAAG AGTTCGAGAA GCGCCTGAGC GAACGTACCA AACTCGTTGC CATCACGCAT ATGTCGAATA CACTCGGCAC GGTGGTCCCG ATCAAGAAGA TCGTGGAGCT TGCCCATGCA CGCGGCATTC CGGTTCTGGT CGATGGCAGT CAGGGTGCAG TGCACCTGCC GGTCGATGTG CAGGATCTGG GCTGTGACTG GTATGTCTTT ACCGGCCACA AAGTCTATGG CCCTTCGGGC ATCGGCGTGC TTTATGGACG CGCGCAAATG CTGGAAAAGA TGCGCCCGTT CCAGGGCGGG GGCGAAATGA TCGAGGAAGT GACGGAGGAA AACGTCACCT ACAATCATCC GCCACACCGC TTTGAAGCAG GCACGCCGCC TATCGTTCAG GCGATTGGTC TGGGTGCGGC GCTTGAATAT ATGGAAAAGA TCGGGCGTCA TGCGATCCTG GCTCACGAGG CAGACTTGCG TGATTATGCG CATGAACGGC TCGGCCGGAT CAACAGCTTG CGCATTTTCG GCAATGCGCC GGACAAGGGC GCAATCATCT CCTTCGCGCT TGAAGGCATT CATGCCCATG ACGTGTCGAT GGTGATCGAT CGGGCGGGGG TCGCGGTGCG CGCGGGAACG CATTGTGCGC AACCGCTCTT GAAACGCTTT GGCGTCACCT CTACATGCCG TGCATCCTTT GCCCTTTATA ATACCCGCGC CGAAGTAGAC GCATTGGCTG AAGCCTTGGA AAAGGCAAGG AAGTTTTTCG GA
Details for BrsuA.01104.a.B1.GE36773
HARVESTED ON:
3/27/2013
SEQUENCED ON:
3/30/2013
EXPECTED MW:
47kDa
OBSERVED MW:
47kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Too many to count (100+)
TOTAL EXPRESSION LEVEL:
Moderate Expression
SOLUBLE EXPRESSION LEVEL
High Expression
EXPRESSION HOST:
data unavailable
SEQUENCING RESULT:
pass with incomplete coverage
PERCENT IDENTITY:
99
PERCENT COVERAGE:
94
Details for BrsuA.01104.a.B1.PS01849
PURIFICATION DATe:
6/27/2013
CONCENTRATION:
67.4mg/ml
OBSERVED MW:
45kDa
EXPRESSION LEVEL:
Moderate Expression
PROTEIN PURIFICATION BUFFER:
20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:
data unavailable
VIAL COUNT (approx.):
5
VIAL VOLUME:
200µl
PERCENT IDENTITY:
99
PERCENT COVERAGE:
94
Details for BrsuA.01104.a.B1.PS01850
PURIFICATION DATe:
6/27/2013
CONCENTRATION:
50.2mg/ml
OBSERVED MW:
data unavailable
EXPRESSION LEVEL:
Moderate Expression
PROTEIN PURIFICATION BUFFER:
20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:
data unavailable
VIAL COUNT (approx.):
5
VIAL VOLUME:
200µl
PERCENT IDENTITY:
99
PERCENT COVERAGE:
94