SSGCID: Target Page

BrsuA.00863.a

Sensor histidine kinase

CENTER ID:	BrsuA.00863.a
ORGANISM:	Brucella suis 1330
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrsuA.00863.a.A3.GE33984	domain2		174	432

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrsuA.00863.a.A3.PW34561	domain2		174	432

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|204722.5.peg.622
RefSeq:	NP_697619.1
UniProt:	A0A0H3GAP1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MVATFISSLY GEAARNNFER LLTAHLFSLV GAVSVSGEGT LQGRPELGDL RYSSPLSGWY WSVDPVTPNL TGKLESPSLV GRIVPEMPVS QAPFDSSFMR SYTLPGLDNE ELSIVETEVV LDNSNRVARF RVMGNLSEVL NEIANFRARL LVYLGVFGIG SILINAAVIL FGLRPLDKVR QALADIREGR SSRLDATLPL EIAPLAREMN ALIENNRRIM ERSRTQVGNL AHSLKTPLSV LVNEARAMGG APGRIVQEQS EAMQVQIQHY LQRARIAAQR DSVVFRTPVT PVLERLHRVT AKLHPTFNIS FRNDLPGAVF AGEREDLEEI IGNLLENAGK WGRKCITIRL AAIAGEQRQF EIVIEDDGPG LEADKIEAAL KRGSRVDETK PGTGLGLAIV QDTVREYGGS LHLGKDALGG LAVRVVLPLT QD

NT Sequence

gtggtcgcca ctttcatcag ctcactctat ggcgaggccg cgcgaaacaa tttcgaacgg cttttgaccg cacatctctt cagcctcgtc ggggcggtca gcgtctcggg cgagggcacg ctacaggggc gccccgaact gggggacctg cgctattcga gcccgctttc gggctggtac tggtccgtcg atccggtaac gcccaatctg accggcaagc ttgaatcgcc ttcgctggtg gggcgcatcg tgccggaaat gcccgtgtca caagccccct tcgacagctc cttcatgcgc agctacacct tgccgggcct cgacaatgaa gagctttcca tcgtcgaaac cgaagtggtg ctggataatt ccaaccgtgt cgcgcgcttt cgcgtcatgg gcaatcttag cgaagtactc aacgaaatag caaatttccg ggcgcggctg cttgtctatc ttggcgtgtt cggcattggc agcattctca tcaatgccgc tgtcattctt ttcggcctgc gcccgctgga taaggttcgt caggcgcttg ccgatatccg cgaagggcgc tcgtccaggc tggatgcaac cttgccgctg gaaatcgcgc cgctggcacg ggaaatgaat gcgctcatag aaaacaatcg ccgcatcatg gagcggtcgc gaacccaggt cggtaatctc gcccattcgc taaaaacgcc gctctccgtt ctggtgaacg aagcccgtgc catgggcggt gcgcccggac gcatcgtgca ggaacaaagc gaggccatgc aggtgcaaat ccagcattat ctgcaacgcg cgcgcatcgc agcacagcgc gatagtgtgg ttttccgaac gcctgtcaca cccgttctgg agcggttgca tcgggtgacg gcaaaattgc atccaacttt caatatttcc ttcaggaacg atcttcccgg tgccgtcttt gcaggcgaac gggaagatct ggaggaaatc atcggcaatc ttctcgaaaa tgccggtaaa tgggggcgca aatgcatcac tatccgcctt gcggcaatcg ccggcgagca aaggcaattt gaaatcgtca ttgaggacga tgggccgggg cttgaggcag acaagattga agcagccctt aaacgcggca gccgcgtcga tgaaacaaag cccggaacgg gtctggggct cgccattgtt caggatacgg tgcgcgaata tggcggcagt ctgcatcttg gcaaggatgc gcttggcgga ttggctgtcc gggtcgtgtt gccgttgacg caagattga

Details for BrsuA.00863.a.A3.GE33984

HARVESTED ON:	11/21/2011
SEQUENCED ON:	11/29/2011
EXPECTED MW:	30kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMRPLDKVRQ ALADIREGRS SRLDATLPLE IAPLAREMNA LIENNRRIME RSRTQVGNLA HSLKTPLSVL VNEARAMGGA PGRIVQEQSE AMQVQIQHYL QRARIAAQRD SVVFRTPVTP VLERLHRVTA KLHPTFNISF RNDLPGAVFA GEREDLEEII GNLLENAGKW GRKCITIRLA AIAGEQRQFE IVIEDDGPGL EADKIEAALK RGSRVDETKP GTGLGLAIVQ DTVREYGGSL HLGKDALGGL AVRVVLPL

Validated NT Sequence

gtcaacggca acacgacccg gacagccaat ccgccaagcg catccttgcc aagatgcaga ctgccgccat attcgcgcac cgtatcctga acaatggcga gccccagacc cgttccgggc tttgtttcat cgacgcggct gccgcgttta agggctgctt caatcttgtc tgcctcaagc cccggcccat cgtcctcaat gacgatttca aattgccttt gctcgccggc gattgccgca aggcggatag tgatgcattt gcgcccccat ttaccggcat tttcgagaag attgccgatg atttcctcca gatcttcccg ttcgcctgca aagacggcac cgggaagatc gttcctgaag gaaatattga aagttggatg caattttgcc gtcacccgat gcaaccgctc cagaacgggt gtgacaggcg ttcggaaaac cacactatcg cgctgtgctg cgatgcgcgc gcgttgcaga taatgctgga tttgcacctg catggcctcg ctttgttcct gcacgatgcg tccgggcgca ccgcccatgg cacgggcttc gttcaccaga acggagagcg gcgtttttag cgaatgggcg agattaccga cctgggttcg cgaccgctcc atgatgcggc gattgttttc tatgagcgca ttcatttccc gtgccagcgg cgcgatttcc agcggcaagg ttgcatccag cctggacgag cgcccttcgc ggatatcggc aagcgcctga cgaaccttat ccagcgggcg catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatggt gatggtgatg agccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgcccgctg gataaggttc gtcaggcgct tgccgatatc cgcgaagggc gctcgtccag gctggatgca accttgccgc tggaaatcgc gccgctggca cgggaaatga atgcgctcat agaaaacaat cgccgcatca tggagcggtc gcgaacccag gtcggtaatc tcgcccattc gctaaaaacg ccgctctccg ttctggtgaa cgaagcccgt gccatgggcg gtgcgcccgg acgcatcgtg caggaacaaa gcgaggccat gcaggtgcaa atccagcatt atctgcaacg cgcgcgcatc gcagcacagc gcgatagtgt ggttttccga acgcctgtca cacccgttct ggagcggttg catcgggtga cggcaaaatt gcatccaact ttcaatattt ccttcaggaa cgatcttccc ggtgccgtct ttgcaggcga acgggaagat ctggaggaaa tcatcggcaa tcttctcgaa aatgccggta aatgggggcg caaatgcatc actatccgcc ttgcggcaat cgccggcgag caaaggcaat ttgaaatcgt cattgaggac gatgggccgg ggcttgaggc agacaagatt gaagcagccc ttaaacgcgg cagccgcgtc gatgaaacaa agcccggaac gggtctgggg ctcgccattg ttcaggatac ggtgcgcgaa tatggcggca gtctgcatct tggcaaggat gcgcttggcg gattggctgt ccgggtcgtg ttgccgttga cgcaagatta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a

Details for BrsuA.00863.a.A3.PW34561

PURIFICATION DATe:	2/28/2012
CONCENTRATION:	15.17mg/ml
OBSERVED MW:	31kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	26
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCGCCCGCTG GATAAGGTTC GTCAGGCGCT TGCCGATATC CGCGAAGGGC GCTCGTCCAG GCTGGATGCA ACCTTGCCGC TGGAAATCGC GCCGCTGGCA CGGGAAATGA ATGCGCTCAT AGAAAACAAT CGCCGCATCA TGGAGCGGTC GCGAACCCAG GTCGGTAATC TCGCCCATTC GCTAAAAACG CCGCTCTCCG TTCTGGTGAA CGAAGCCCGT GCCATGGGCG GTGCGCCCGG ACGCATCGTG CAGGAACAAA GCGAGGCCAT GCAGGTGCAA ATCCAGCATT ATCTGCAACG CGCGCGCATC GCAGCACAGC GCGATAGTGT GGTTTTCCGA ACGCCTGTCA CACCCGTTCT GGAGCGGTTG CATCGGGTGA CGGCAAAATT GCATCCAACT TTCAATATTT CCTTCAGGAA CGATCTTCCC GGTGCCGTCT TTGCAGGCGA ACGGGAAGAT CTGGAGGAAA TCATCGGCAA TCTTCTCGAA AATGCCGGTA AATGGGGGCG CAAATGCATC ACTATCCGCC TTGCGGCAAT CGCCGGCGAG CAAAGGCAAT TTGAAATCGT CATTGAGGAC GATGGGCCGG GGCTTGAGGC AGACAAGATT GAAGCAGCCC TTAAACGCGG CAGCCGCGTC GATGAAACAA AGCCCGGAAC GGGTCTGGGG CTCGCCATTG TTCAGGATAC GGTGCGCGAA TATGGCGGCA GTCTGCATCT TGGCAAGGAT GCGCTTGGCG GATTGGCTGT CCGGGTCGTG TTGCCGTTGA CGCAAGATTA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A