BrsuA.00808.a

L-asparaginase (EC 3.5.1.1)

CENTER ID: BrsuA.00808.a
ORGANISM: Brucella suis 1330
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrsuA.00808.a.B1.GE36771 Full length( BrsuA.00808.a ) 1 305
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruSui107850_1997
BV-BRC: fig|204722.5.peg.1997
RefSeq: NP_698938.1
RefSeq: YP_005616762.1
UniProt: A0A0H3GDI6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPTLTADALI AAVPQIRNYA QIKAQSLFQL PSPSLKFRHG LEVIGWAENE VKSGADGIVL TQGTDTLEEM AFLLDLLWEH PQPLVLTGAM RSPRAPGADG PANLVAAVLT AASRLSRERG VLVAMNDTIH AARWITKSHA LSVQTFVSSD SGPLGYVYEE TPVYINTLSR LPKIDRTQFN ADVKIGFYES LLDGDAQMLR SMFESGRYDG IVVACFGAGH VSGDEADIIE RYASRIPVVV ASRTYGGRTA TKTYGFHGSE IDLQTKGAML AGWLSPRKAR LLLWALLAAG KNLEETRANF LLYNP
NT Sequence
GTGCCGACCC TGACCGCGGA TGCACTCATT GCCGCTGTGC CGCAGATTAG AAATTATGCG CAGATCAAGG CCCAATCTCT TTTTCAGCTT CCCAGCCCCT CGCTAAAATT TCGGCACGGC CTTGAAGTGA TAGGCTGGGC TGAAAACGAG GTGAAATCGG GAGCGGACGG CATAGTGCTG ACGCAGGGAA CCGATACGCT TGAGGAAATG GCATTCCTTC TGGATTTATT GTGGGAACAT CCGCAGCCCC TCGTACTGAC CGGCGCCATG CGCTCGCCGC GCGCGCCGGG TGCTGACGGT CCGGCCAATC TGGTGGCGGC CGTATTGACA GCCGCGAGCA GGCTTAGCCG GGAACGCGGT GTCCTTGTTG CAATGAACGA TACAATCCAC GCGGCACGTT GGATAACAAA ATCTCATGCG CTCAGTGTCC AGACATTCGT ATCGTCGGAC AGCGGGCCGT TGGGATATGT GTATGAGGAG ACGCCGGTTT ACATCAACAC CCTTTCGCGG TTGCCAAAAA TTGACCGTAC GCAGTTCAAC GCCGATGTGA AGATCGGCTT TTACGAATCG CTGCTGGATG GTGACGCGCA GATGCTGCGA AGCATGTTTG AGAGCGGCAG ATATGATGGA ATCGTCGTCG CCTGTTTTGG AGCAGGACAT GTGTCGGGCG ACGAAGCCGA CATTATCGAG CGCTATGCGT CCCGCATTCC GGTCGTCGTA GCCAGCCGGA CCTATGGCGG AAGGACCGCC ACGAAGACTT ATGGGTTCCA TGGCTCCGAG ATTGATCTTC AGACGAAAGG CGCTATGCTC GCAGGCTGGT TATCCCCCCG CAAGGCGCGG TTATTGCTGT GGGCACTACT GGCAGCGGGT AAAAATCTGG AAGAAACAAG AGCTAACTTC CTGCTCTATA ACCCA
Details for BrsuA.00808.a.B1.GE36771
HARVESTED ON: 3/27/2013
SEQUENCED ON: 3/30/2013
EXPECTED MW: 34kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 98
PERCENT COVERAGE: 122
Validated AA Sequence
MAHHHHHHMV PTLTADALIA AVPQIRNYAQ IKAQSLFQLP SPSLKFRHGL EVIGWAENEV KSGADGIVLT QGTDTLEEMA FLLDLLWEHP QPLVLTGAMR SPRAPGADGP ANLVAAVLTA ASRLSRERGV LVAMNDTIHA ARWITKSHAL SVQTFVSSDS GPLGYVYEET PVYINTLSRL PKIDRTQFNA DVKIGFYESL LDGDAQMLRS MFESGRYDGI VVACFGAGHV SGDEXDIIER YASRIPVVVA SRTYGGRTAT KTYGFHGSEI DLQTKGAMLA XWLSPRKARL LLWALLAAGK NLEEXXXNFL LYXPXVSXXX SXXXXPXXXX XXKXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XX
Validated NT Sequence
ttnnttactt taagaaggag anataccatg gctcaccacc accaccacca tatggtgccg accctgaccg cggatgcact cattgccgct gtgccgcaga ttagaaatta tgcgcagatc aaggcccaat ctctttttca gcttcccagc ccctcgctaa aatttcggca cggccttgaa gtgataggct gggctgaaaa cgaggtgaaa tcgggagcgg acggcatagt gctgacgcag ggaaccgata cgcttgagga aatggcattc cttctggatt tattgtggga acatccgcag cccctcgtac tgaccggcgc catgcgctcg ccgcgcgcgc cgggtgctga cggtccggcc aatctggtgg cggccgtatt gacagccgcg agcaggctta gccgggaacg cggtgtcctt gttgcaatga acgatacaat ccacgcggca cgttggataa caaaatctca tgcgctcagt gtccagacat tcgtatcgtc ggacagcggg ccgttgggat atgtgtatga ggagacgccg gtttacatca acaccctttc gcggttgcca aaaattgacc gtacgcagtt caacgccgat gtgaagatcg gcttttacga atcgctgctg gatggtgacg cgcagatgct gcgaagcatg tttgagagcg gcagatatga tggaatcgtc gtcgcctgtt ttggagcagg acatgtgtcg ggcgacgaan ccgacattat cgagcgctat gcgtcccgca ttccggtcgt cgtagccagc cggacctatg gcggaaggac cgccacgaag acttatgggt tccatggctc cgagattgat cttcagacga aaggcgctat gctcgcangc tggttatccc cccgcaaggc gcggttattg ctgtgggcac tactggcagc gggtaaaaat ctggaagaaa nnananctaa cttcctgctc tatnacccat nagtgagtaa nanngnatcn nnnnnnnann nncccnnnnn nnnnnnnnnn nnaaaaaann nnttngncnn cnnnnnnnnn nngnnnnnct nnnnnnnnnc nnnnncnnnn nnnnncnnnn nnnnnnnnnc nnnnnnnnnn nnnnnctnnn nnnnnngnnn nnnnnnnnnn nnnnnnncnn nnnnnncnnn nnnnncnnnn nnncc
Expected Protein Sequence
MAHHHHHHMP TLTADALIAA VPQIRNYAQI KAQSLFQLPS PSLKFRHGLE VIGWAENEVK SGADGIVLTQ GTDTLEEMAF LLDLLWEHPQ PLVLTGAMRS PRAPGADGPA NLVAAVLTAA SRLSRERGVL VAMNDTIHAA RWITKSHALS VQTFVSSDSG PLGYVYEETP VYINTLSRLP KIDRTQFNAD VKIGFYESLL DGDAQMLRSM FESGRYDGIV VACFGAGHVS GDEADIIERY ASRIPVVVAS RTYGGRTATK TYGFHGSEID LQTKGAMLAG WLSPRKARLL LWALLAAGKN LEETRANFLL YNP
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgccga ccctgaccgc ggatgcactc attgccgctg tgccgcagat tagaaattat gcgcagatca aggcccaatc tctttttcag cttcccagcc cctcgctaaa atttcggcac ggccttgaag tgataggctg ggctgaaaac gaggtgaaat cgggagcgga cggcatagtg ctgacgcagg gaaccgatac gcttgaggaa atggcattcc ttctggattt attgtgggaa catccgcagc ccctcgtact gaccggcgcc atgcgctcgc cgcgcgcgcc gggtgctgac ggtccggcca atctggtggc ggccgtattg acagccgcga gcaggcttag ccgggaacgc ggtgtccttg ttgcaatgaa cgatacaatc cacgcggcac gttggataac aaaatctcat gcgctcagtg tccagacatt cgtatcgtcg gacagcgggc cgttgggata tgtgtatgag gagacgccgg tttacatcaa caccctttcg cggttgccaa aaattgaccg tacgcagttc aacgccgatg tgaagatcgg cttttacgaa tcgctgctgg atggtgacgc gcagatgctg cgaagcatgt ttgagagcgg cagatatgat ggaatcgtcg tcgcctgttt tggagcagga catgtgtcgg gcgacgaagc cgacattatc gagcgctatg cgtcccgcat tccggtcgtc gtagccagcc ggacctatgg cggaaggacc gccacgaaga cttatgggtt ccatggctcc gagattgatc ttcagacgaa aggcgctatg ctcgcaggct ggttatcccc ccgcaaggcg cggttattgc tgtgggcact actggcagcg ggtaaaaatc tggaagaaac aagagctaac ttcctgctct ataacccatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat