BrsuA.00674.b

Aspartate aminotransferase (EC 2.6.1.1)

CENTER ID: BrsuA.00674.b
ORGANISM: Brucella suis 1330
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrsuA.00674.b.B1.GE36770 Full length( BrsuA.00674.b ) 1 372
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruSui107850_0926
BV-BRC: fig|204722.5.peg.926
RefSeq: NP_697924.1
RefSeq: YP_005615748.1
UniProt: A0A0H3G2Y2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDVLAEANRR RAAGHPIISM AVGQPADSAP VVVRQAAERA LKDGRIGYTD ALGLIELREA IAAHYADHYG VSVSPERIAV TTGSSAAFNL AFIVYFDPGD RVAITRPGYP AYRNILTALG LEVVEIPTGH GTFSSLTAQA LAAAHAEKPL KGVLFASPAN PTGAVIDKPE LTAIIETARN LGIRVISDEI YHRLSYEADD VTALEVADDV TIINSFSKYY CMTGWRIGWM VLPDNDIRAI ERLSQSLYIS APELSQRGAI EAFRATAELE RIKDRYRRNR LILLNHLPRM GLDLAAPMDG AFYAYCDVSR FTNDSMEFVR GMISEINIAA TPGRDFDPLD GHRTVRFSYA GKVEEMEEAV RRLDDWLPQQ KA
NT Sequence
ATGGACGTTC TGGCTGAGGC GAACCGTCGG CGCGCGGCGG GCCATCCGAT CATTTCCATG GCGGTTGGCC AACCGGCTGA TTCCGCACCT GTGGTCGTTC GTCAGGCCGC AGAGCGCGCG TTGAAAGATG GCCGCATCGG CTATACCGAC GCTTTGGGCC TGATTGAACT GCGCGAGGCT ATCGCCGCGC ATTATGCGGA TCATTACGGC GTCAGCGTGT CGCCGGAGCG GATTGCGGTC ACGACCGGCT CTTCCGCCGC TTTCAATCTT GCATTTATTG TCTATTTCGA TCCCGGCGAC CGGGTGGCGA TAACGCGCCC CGGCTATCCG GCCTACCGCA ACATCTTGAC GGCGCTTGGT CTCGAAGTGG TGGAAATTCC CACCGGCCAT GGAACATTCT CGTCGCTGAC GGCGCAAGCA CTTGCCGCCG CCCATGCTGA AAAACCCTTG AAGGGCGTTC TCTTTGCAAG CCCCGCCAAC CCAACCGGAG CGGTTATCGA CAAACCGGAA CTGACGGCGA TCATCGAGAC GGCACGTAAC CTCGGAATCC GGGTCATATC GGATGAAATC TATCATCGCC TGTCCTATGA AGCCGACGAT GTAACCGCAC TTGAAGTTGC CGACGACGTC ACCATCATCA ATTCCTTCTC CAAATATTAC TGCATGACGG GCTGGCGCAT CGGCTGGATG GTTCTGCCGG ACAACGATAT CCGGGCGATT GAGCGCCTCA GCCAGAGCCT CTATATTTCC GCGCCCGAAC TGTCGCAGCG CGGCGCCATT GAGGCCTTCC GCGCCACTGC GGAACTGGAA CGGATCAAGG ATCGCTATCG CCGAAACAGG CTTATCCTGC TCAATCATCT GCCGCGCATG GGGCTTGATC TGGCTGCACC GATGGACGGC GCATTCTACG CCTATTGCGA TGTGTCGCGT TTCACCAATG ACAGCATGGA GTTTGTGCGC GGGATGATCT CCGAGATCAA TATCGCCGCC ACCCCCGGCC GTGATTTCGA TCCGCTGGAC GGGCATCGCA CCGTGCGTTT CTCCTACGCA GGCAAGGTCG AGGAAATGGA AGAGGCTGTT CGGCGGCTTG ACGACTGGTT GCCGCAGCAG AAAGCG
Details for BrsuA.00674.b.B1.GE36770
HARVESTED ON: 3/27/2013
SEQUENCED ON: 3/30/2013
EXPECTED MW: 42kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 97
PERCENT COVERAGE: 84
Validated AA Sequence
MAHHHHHHMD VLAEANRRRA AGHPIISMAV GQPADSAPVV VRQAAERALK DGRIGYTDAL GLIELREAIA AHYADHYGVS VSPERIAVTT GSSAAFNLAF IVYFDPGDRV AITRPGYPAY RNILTALGLE VVEIPTGHGT FSSLTAQALA AAHAEKPLKG VLFASPANPT GAVIDKPELT AIIETARNLG IRVISDEIYH RLSYEADDVT ALEVADDVTI INSFSKYYCM TGWRIGWMVL PDNDIRAIER LSQSLYISAP ELSQRGAIEA FRATAELERI KXRYRRNRLI LLNHLPRMGL DLAAPMDGXF YAXXDVSXXX
Validated NT Sequence
attttgtnnn ntttaagaag gaganatacc atggctcacc accaccacca ccatatggac gttctggctg aggcgaaccg tcggcgcgcg gcgggccatc cgatcatttc catggcggtt ggccaaccgg ctgattccgc acctgtggtc gttcgtcagg ccgcagagcg cgcgttgaaa gatggccgca tcggctatac cgacgctttg ggcctgattg aactgcgcga ggctatcgcc gcgcattatg cggatcatta cggcgtcagc gtgtcgccgg agcggattgc ggtcacgacc ggctcttccg ccgctttcaa tcttgcattt attgtctatt tcgatcccgg cgaccgggtg gcgataacgc gccccggcta tccggcctac cgcaacatct tgacggcgct tggtctcgaa gtggtggaaa ttcccaccgg ccatggaaca ttctcgtcgc tgacggcgca agcacttgcc gccgcccatg ctgaaaaacc cttgaagggc gttctctttg caagccccgc caacccaacc ggagcggtta tcgacaaacc ggaactgacg gcgatcatcg agacggcacg taacctcgga atccgggtca tatcggatga aatctatcat cgcctgtcct atgaagccga cgatgtaacc gcacttgaag ttgccgacga cgtcaccatc atcaattcct tctccaaata ttactgcatg acgggctggc gcatcggctg gatggttctg ccggacaacg atatccgggc gattgagcgc ctcagccaga gcctctatat ttccgcgccc gaactgtcgc agcgcggcgc cattgaggcc ttccgcgcca ctgcggaact ggaacggatc aaggntcgct atcgccgaaa caggcttatc ctgctcaatc atctgccgcg catggggctt gatctggctg caccgatgga cggcncattc tacgcctnnt ncgatgtgtc gnttcnncnn tgacagcatg gnnttngngc nnnggnngnt cnncnanatn annnncgccn nnncccccnn nnnnnattnn atccnnnnna nnggcannnn nnnnnnttnt nnnnnnnnng nnngnnnnnn aannnnaagn nnnnngnnnn tnnnnnnnnn nnncnnnnnn nnnnnnnt
Expected Protein Sequence
MAHHHHHHMD VLAEANRRRA AGHPIISMAV GQPADSAPVV VRQAAERALK DGRIGYTDAL GLIELREAIA AHYADHYGVS VSPERIAVTT GSSAAFNLAF IVYFDPGDRV AITRPGYPAY RNILTALGLE VVEIPTGHGT FSSLTAQALA AAHAEKPLKG VLFASPANPT GAVIDKPELT AIIETARNLG IRVISDEIYH RLSYEADDVT ALEVADDVTI INSFSKYYCM TGWRIGWMVL PDNDIRAIER LSQSLYISAP ELSQRGAIEA FRATAELERI KDRYRRNRLI LLNHLPRMGL DLAAPMDGAF YAYCDVSRFT NDSMEFVRGM ISEINIAATP GRDFDPLDGH RTVRFSYAGK VEEMEEAVRR LDDWLPQQKA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggacg ttctggctga ggcgaaccgt cggcgcgcgg cgggccatcc gatcatttcc atggcggttg gccaaccggc tgattccgca cctgtggtcg ttcgtcaggc cgcagagcgc gcgttgaaag atggccgcat cggctatacc gacgctttgg gcctgattga actgcgcgag gctatcgccg cgcattatgc ggatcattac ggcgtcagcg tgtcgccgga gcggattgcg gtcacgaccg gctcttccgc cgctttcaat cttgcattta ttgtctattt cgatcccggc gaccgggtgg cgataacgcg ccccggctat ccggcctacc gcaacatctt gacggcgctt ggtctcgaag tggtggaaat tcccaccggc catggaacat tctcgtcgct gacggcgcaa gcacttgccg ccgcccatgc tgaaaaaccc ttgaagggcg ttctctttgc aagccccgcc aacccaaccg gagcggttat cgacaaaccg gaactgacgg cgatcatcga gacggcacgt aacctcggaa tccgggtcat atcggatgaa atctatcatc gcctgtccta tgaagccgac gatgtaaccg cacttgaagt tgccgacgac gtcaccatca tcaattcctt ctccaaatat tactgcatga cgggctggcg catcggctgg atggttctgc cggacaacga tatccgggcg attgagcgcc tcagccagag cctctatatt tccgcgcccg aactgtcgca gcgcggcgcc attgaggcct tccgcgccac tgcggaactg gaacggatca aggatcgcta tcgccgaaac aggcttatcc tgctcaatca tctgccgcgc atggggcttg atctggctgc accgatggac ggcgcattct acgcctattg cgatgtgtcg cgtttcacca atgacagcat ggagtttgtg cgcgggatga tctccgagat caatatcgcc gccacccccg gccgtgattt cgatccgctg gacgggcatc gcaccgtgcg tttctcctac gcaggcaagg tcgaggaaat ggaagaggct gttcggcggc ttgacgactg gttgccgcag cagaaagcgt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat