BrsuA.00323.c

ZINC PROTEASE (EC 3.4.99.-)

CENTER ID: BrsuA.00323.c
ORGANISM: Brucella suis 1330
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrsuA.00323.c.B1.GE36768 Full length( BrsuA.00323.c ) 1 346
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrsuA.00323.c.B1.PS01905 Full length( BrsuA.00323.c ) 1 346

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruSui107850_2425

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKERIDNLGA EMSFSASQDS VSGGVRMLAE NRDAVTDLVA LAVNEPRFDQ EAIDRIRQQI VAGIEAAQRN PSTIASRKFA EVLYGNHPYA RDDEGTVKSL QSISRDDLAN FHRKNFARDK LTVGVVGAIN AKDLGVMLDR IFGDLPASAE LVPVPDAKLA LGTTTSLNFD MPQTSISFVY PAIPRKDPEF FAAYLMNHIL GGGFTSRLYN EVREKRGLAY SVSSSMVMHD HVSELMISTA TRPDKAQDSL KIIREQVAAM ANDGPTEEEL AAAKSFLKGS YAVNNLDSSG AIANTLVSLQ EAGLPSDYID KRSELIDAVT LDQVKAIARK LLQAEPAILI YGPAQS
NT Sequence
ATGAAGGAGC GGATCGATAA TCTGGGCGCG GAAATGAGTT TTTCCGCTTC GCAGGATTCG GTTTCCGGCG GCGTCCGTAT GCTGGCCGAA AATCGTGATG CCGTGACCGA TCTCGTGGCG CTTGCCGTCA ACGAGCCGCG CTTCGATCAG GAAGCCATCG ACCGCATTCG CCAGCAGATT GTTGCAGGCA TTGAGGCCGC ACAGCGCAAT CCGTCTACGA TTGCGTCGCG CAAGTTTGCC GAAGTGCTTT ACGGCAACCA CCCTTATGCG CGTGACGATG AGGGAACCGT GAAATCCCTG CAATCGATCA GCCGCGATGA TCTTGCGAAT TTCCATCGCA AGAATTTCGC CCGCGACAAG CTGACCGTTG GCGTTGTGGG TGCCATCAAC GCCAAGGATC TGGGCGTCAT GCTCGACAGG ATTTTTGGCG ACCTGCCCGC CTCGGCGGAA CTGGTTCCGG TGCCGGATGC GAAGCTTGCG CTTGGCACCA CCACCAGCCT GAATTTCGAT ATGCCGCAGA CGTCGATCAG CTTCGTCTAC CCGGCCATTC CGCGCAAAGA CCCTGAGTTT TTTGCCGCCT ATCTGATGAA CCATATTCTG GGCGGCGGTT TCACCTCGCG TCTTTATAAC GAAGTGCGTG AAAAGCGCGG CCTTGCCTAT TCGGTGTCCT CGTCCATGGT CATGCACGAC CATGTTTCCG AACTGATGAT TTCCACCGCC ACCCGGCCTG ACAAGGCCCA GGACTCATTG AAAATCATTC GTGAGCAGGT TGCCGCCATG GCCAATGACG GCCCGACCGA GGAAGAGCTT GCCGCAGCCA AGAGCTTTCT CAAAGGCTCC TATGCGGTGA ACAATCTCGA TTCGTCGGGA GCTATTGCCA ATACGCTGGT GAGTTTGCAG GAAGCCGGGC TTCCAAGCGA TTATATCGAC AAGCGTTCCG AACTCATCGA TGCTGTCACG CTGGATCAGG TCAAGGCCAT TGCCAGGAAA CTGCTTCAGG CCGAGCCTGC GATCCTGATC TATGGACCGG CGCAAAGT
Details for BrsuA.00323.c.B1.GE36768
HARVESTED ON: 3/27/2013
SEQUENCED ON: 3/30/2013
EXPECTED MW: 39kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 92
Validated AA Sequence
MSFSASQDSV SGGVRMLAEN RDAVTDLVAL AVNEPRFDQE AIDRIRQQIV AGIEAAQRNP STIASRKFAE VLYGNHPYAR DDEGTVKSLQ SISRDDLANF HRKNFARDKL TVGVVGAINA KDLGVMLDRI FGDLPASAEL VPVPDAKLAL GTTTSLNFDM PQTSISFVYP AIPRKDPEFF AAYLMNHILG GGFTSRLYNE VREKRGLAYS VSSSMVMHDH VSELMISTAT RPDKAQDSLK IIREQVAAMA NDGPTEEELA AAKSFLKGSY AVNNLDSSGA IANTLVSLQE AGLPSDYIDK RSELIDAVTL DQVKAIARKL LQAEPAI
Validated NT Sequence
ggatcgcagg ctcggcctga agcagtttcc tggcaatggc cttgacctga tccagcgtga cagcatcgat gagttcggaa cgcttgtcga tataatcgct tggaagcccg gcttcctgca aactcaccag cgtattggca atagctcccg acgaatcgag attgttcacc gcataggagc ctttgagaaa gctcttggct gcggcaagct cttcctcggt cgggccgtca ttggccatgg cggcaacctg ctcacgaatg attttcaatg agtcctgggc cttgtcaggc cgggtggcgg tggaaatcat cagttcggaa acatggtcgt gcatgaccat ggacgaggac accgaatagg caaggccgcg cttttcacgc acttcgttat aaagacgcga ggtgaaaccg ccgcccagaa tatggttcat cagataggcg gcaaaaaact cagggtcttt gcgcggaatg gccgggtaga cgaagctgat cgacgtctgc ggcatatcga aattcaggct ggtggtggtg ccaagcgcaa gcttcgcatc cggcaccgga accagttccg ccgaggcggg caggtcgcca aaaatcctgt cgagcatgac gcccagatcc ttggcgttga tggcacccac aacgccaacg gtcagcttgt cgcgggcgaa attcttgcga tggaaattcg caagatcatc gcggctgatc gattgcaggg atttcacggt tccctcatcg tcacgcgcat aagggtggtt gccgtaaagc acttcggcaa acttgcgcga cgcaatcgta gacggattgc gctgtgcggc ctcaatgcct gcaacaatct gctggcgaat gcggtcgatg gcttcctgat cgaagcgcgg ctcgttgacg gcaagcgcca cgagatcggt cacggcatca cgattttcgg ccagcatacg gacgccgccg gaaaccgaat cctgcgaagc ggaaaaactc atttccgcgc ccagattatc gatccgct
Expected Protein Sequence
MAHHHHHHMK ERIDNLGAEM SFSASQDSVS GGVRMLAENR DAVTDLVALA VNEPRFDQEA IDRIRQQIVA GIEAAQRNPS TIASRKFAEV LYGNHPYARD DEGTVKSLQS ISRDDLANFH RKNFARDKLT VGVVGAINAK DLGVMLDRIF GDLPASAELV PVPDAKLALG TTTSLNFDMP QTSISFVYPA IPRKDPEFFA AYLMNHILGG GFTSRLYNEV REKRGLAYSV SSSMVMHDHV SELMISTATR PDKAQDSLKI IREQVAAMAN DGPTEEELAA AKSFLKGSYA VNNLDSSGAI ANTLVSLQEA GLPSDYIDKR SELIDAVTLD QVKAIARKLL QAEPAILIYG PAQS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaagg agcggatcga taatctgggc gcggaaatga gtttttccgc ttcgcaggat tcggtttccg gcggcgtccg tatgctggcc gaaaatcgtg atgccgtgac cgatctcgtg gcgcttgccg tcaacgagcc gcgcttcgat caggaagcca tcgaccgcat tcgccagcag attgttgcag gcattgaggc cgcacagcgc aatccgtcta cgattgcgtc gcgcaagttt gccgaagtgc tttacggcaa ccacccttat gcgcgtgacg atgagggaac cgtgaaatcc ctgcaatcga tcagccgcga tgatcttgcg aatttccatc gcaagaattt cgcccgcgac aagctgaccg ttggcgttgt gggtgccatc aacgccaagg atctgggcgt catgctcgac aggatttttg gcgacctgcc cgcctcggcg gaactggttc cggtgccgga tgcgaagctt gcgcttggca ccaccaccag cctgaatttc gatatgccgc agacgtcgat cagcttcgtc tacccggcca ttccgcgcaa agaccctgag ttttttgccg cctatctgat gaaccatatt ctgggcggcg gtttcacctc gcgtctttat aacgaagtgc gtgaaaagcg cggccttgcc tattcggtgt cctcgtccat ggtcatgcac gaccatgttt ccgaactgat gatttccacc gccacccggc ctgacaaggc ccaggactca ttgaaaatca ttcgtgagca ggttgccgcc atggccaatg acggcccgac cgaggaagag cttgccgcag ccaagagctt tctcaaaggc tcctatgcgg tgaacaatct cgattcgtcg ggagctattg ccaatacgct ggtgagtttg caggaagccg ggcttccaag cgattatatc gacaagcgtt ccgaactcat cgatgctgtc acgctggatc aggtcaaggc cattgccagg aaactgcttc aggccgagcc tgcgatcctg atctatggac cggcgcaaag ttgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for BrsuA.00323.c.B1.PS01905
PURIFICATION DATe: 9/12/2013
CONCENTRATION: 11.5mg/ml
OBSERVED MW: 38kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 92
Protocol Notes
notes unavailable
Validated AA Sequence
MSFSASQDSV SGGVRMLAEN RDAVTDLVAL AVNEPRFDQE AIDRIRQQIV AGIEAAQRNP STIASRKFAE VLYGNHPYAR DDEGTVKSLQ SISRDDLANF HRKNFARDKL TVGVVGAINA KDLGVMLDRI FGDLPASAEL VPVPDAKLAL GTTTSLNFDM PQTSISFVYP AIPRKDPEFF AAYLMNHILG GGFTSRLYNE VREKRGLAYS VSSSMVMHDH VSELMISTAT RPDKAQDSLK IIREQVAAMA NDGPTEEELA AAKSFLKGSY AVNNLDSSGA IANTLVSLQE AGLPSDYIDK RSELIDAVTL DQVKAIARKL LQAEPAI
Validated NT Sequence
ggatcgcagg ctcggcctga agcagtttcc tggcaatggc cttgacctga tccagcgtga cagcatcgat gagttcggaa cgcttgtcga tataatcgct tggaagcccg gcttcctgca aactcaccag cgtattggca atagctcccg acgaatcgag attgttcacc gcataggagc ctttgagaaa gctcttggct gcggcaagct cttcctcggt cgggccgtca ttggccatgg cggcaacctg ctcacgaatg attttcaatg agtcctgggc cttgtcaggc cgggtggcgg tggaaatcat cagttcggaa acatggtcgt gcatgaccat ggacgaggac accgaatagg caaggccgcg cttttcacgc acttcgttat aaagacgcga ggtgaaaccg ccgcccagaa tatggttcat cagataggcg gcaaaaaact cagggtcttt gcgcggaatg gccgggtaga cgaagctgat cgacgtctgc ggcatatcga aattcaggct ggtggtggtg ccaagcgcaa gcttcgcatc cggcaccgga accagttccg ccgaggcggg caggtcgcca aaaatcctgt cgagcatgac gcccagatcc ttggcgttga tggcacccac aacgccaacg gtcagcttgt cgcgggcgaa attcttgcga tggaaattcg caagatcatc gcggctgatc gattgcaggg atttcacggt tccctcatcg tcacgcgcat aagggtggtt gccgtaaagc acttcggcaa acttgcgcga cgcaatcgta gacggattgc gctgtgcggc ctcaatgcct gcaacaatct gctggcgaat gcggtcgatg gcttcctgat cgaagcgcgg ctcgttgacg gcaagcgcca cgagatcggt cacggcatca cgattttcgg ccagcatacg gacgccgccg gaaaccgaat cctgcgaagc ggaaaaactc atttccgcgc ccagattatc gatccgct
Expressed Protein Sequence
MAHHHHHHMK ERIDNLGAEM SFSASQDSVS GGVRMLAENR DAVTDLVALA VNEPRFDQEA IDRIRQQIVA GIEAAQRNPS TIASRKFAEV LYGNHPYARD DEGTVKSLQS ISRDDLANFH RKNFARDKLT VGVVGAINAK DLGVMLDRIF GDLPASAELV PVPDAKLALG TTTSLNFDMP QTSISFVYPA IPRKDPEFFA AYLMNHILGG GFTSRLYNEV REKRGLAYSV SSSMVMHDHV SELMISTATR PDKAQDSLKI IREQVAAMAN DGPTEEELAA AKSFLKGSYA VNNLDSSGAI ANTLVSLQEA GLPSDYIDKR SELIDAVTLD QVKAIARKLL QAEPAILIYG PAQS
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAAGG AGCGGATCGA TAATCTGGGC GCGGAAATGA GTTTTTCCGC TTCGCAGGAT TCGGTTTCCG GCGGCGTCCG TATGCTGGCC GAAAATCGTG ATGCCGTGAC CGATCTCGTG GCGCTTGCCG TCAACGAGCC GCGCTTCGAT CAGGAAGCCA TCGACCGCAT TCGCCAGCAG ATTGTTGCAG GCATTGAGGC CGCACAGCGC AATCCGTCTA CGATTGCGTC GCGCAAGTTT GCCGAAGTGC TTTACGGCAA CCACCCTTAT GCGCGTGACG ATGAGGGAAC CGTGAAATCC CTGCAATCGA TCAGCCGCGA TGATCTTGCG AATTTCCATC GCAAGAATTT CGCCCGCGAC AAGCTGACCG TTGGCGTTGT GGGTGCCATC AACGCCAAGG ATCTGGGCGT CATGCTCGAC AGGATTTTTG GCGACCTGCC CGCCTCGGCG GAACTGGTTC CGGTGCCGGA TGCGAAGCTT GCGCTTGGCA CCACCACCAG CCTGAATTTC GATATGCCGC AGACGTCGAT CAGCTTCGTC TACCCGGCCA TTCCGCGCAA AGACCCTGAG TTTTTTGCCG CCTATCTGAT GAACCATATT CTGGGCGGCG GTTTCACCTC GCGTCTTTAT AACGAAGTGC GTGAAAAGCG CGGCCTTGCC TATTCGGTGT CCTCGTCCAT GGTCATGCAC GACCATGTTT CCGAACTGAT GATTTCCACC GCCACCCGGC CTGACAAGGC CCAGGACTCA TTGAAAATCA TTCGTGAGCA GGTTGCCGCC ATGGCCAATG ACGGCCCGAC CGAGGAAGAG CTTGCCGCAG CCAAGAGCTT TCTCAAAGGC TCCTATGCGG TGAACAATCT CGATTCGTCG GGAGCTATTG CCAATACGCT GGTGAGTTTG CAGGAAGCCG GGCTTCCAAG CGATTATATC GACAAGCGTT CCGAACTCAT CGATGCTGTC ACGCTGGATC AGGTCAAGGC CATTGCCAGG AAACTGCTTC AGGCCGAGCC TGCGATCCTG ATCTATGGAC CGGCGCAAAG TTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT