BrsuA.00168.a

2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (EC 2.7.7.60) / 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (EC 4.6.1.12)

CENTER ID:	BrsuA.00168.a
ORGANISM:	Brucella suis 1330
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrsuA.00168.a.B1.GE36766	Full length( BrsuA.00168.a )		1	447

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrsuA.00168.a.B1.PS01895	Full length( BrsuA.00168.a )		1	447

External Resources

RESOURCE	REFERENCE ID
PATRIC ID:	fig\|204722.5.peg.1140
RefSeq:	NP_698125.2
RefSeq:	YP_005615950.1
UniProt:	Q8G0H4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKSAYSLITP LSERKEKQPE RTINQLPSGI CRRKALFGLQ AQSLAEVTVI SKAAAVIVAA GRGERAGQSA EGPKQYRLIG AEAVLARTLR AFTDCPLIGT IAVVIHPDDH ALYRRAVPEK HENVILVTGG PTRQESTRLG LLALKDEAPQ YVLIHDGVRP FIGQDLLERI IANLTPDNGV LPALAVPDTL KRAAADGMVE TTISRTGLFA AQTPQAFPYA PILDAHEKAF AINRTDFTDD AAIAEWQEIA VRIIEGSADN TKLTWAKDIE MADKRLRQDH AVFPDIRTGN GYDVHSFEPG DHVTLCGVKI PHEAKLNGHS DADVALHALT DALLATRGAG DIGTHFPPSD SQWKGAASRI FIEHAAKIVR EAGGRIANVD VTLISEAPKI GPHRAAMTQA LCDMLGIAAD RVSIKATTNE KLGFVGRREG IAAIATATVI YPGEVPE

NT Sequence

ATGAAATCCG CATACAGTTT AATCACCCCG CTTTCCGAAC GCAAGGAAAA GCAGCCTGAA CGCACGATAA ATCAATTGCC GTCTGGCATT TGTCGTCGCA AGGCCTTATT CGGCTTGCAG GCACAATCTT TGGCAGAGGT TACAGTCATT TCAAAGGCAG CAGCAGTCAT CGTGGCGGCC GGTCGCGGCG AGCGGGCGGG GCAAAGCGCC GAGGGGCCGA AGCAATATCG GCTCATTGGC GCAGAGGCCG TGCTTGCCCG CACTTTGCGC GCTTTTACCG ATTGCCCGCT CATCGGCACA ATCGCCGTCG TCATCCATCC CGACGATCAT GCGCTTTATC GGCGCGCTGT GCCGGAGAAG CATGAGAACG TCATCCTCGT TACCGGTGGC CCCACCCGAC AGGAATCGAC CCGCCTCGGT CTTCTGGCCT TGAAGGACGA AGCGCCGCAA TATGTGTTGA TCCATGATGG CGTGCGTCCT TTCATCGGTC AGGATTTGCT GGAACGCATC ATTGCAAACC TCACCCCTGA TAATGGTGTG CTTCCCGCCC TCGCCGTCCC CGACACGTTG AAACGGGCGG CGGCAGACGG CATGGTAGAG ACCACCATTT CCCGTACCGG CTTGTTCGCG GCGCAGACGC CGCAAGCTTT TCCCTATGCG CCGATCTTGG ACGCACATGA GAAAGCCTTC GCAATCAACC GCACCGACTT TACCGACGAT GCCGCCATTG CCGAATGGCA GGAAATTGCT GTTCGCATTA TCGAAGGTTC GGCGGATAAT ACAAAGCTCA CCTGGGCAAA GGATATTGAA ATGGCAGACA AGCGCCTGCG GCAGGACCAT GCCGTATTTC CCGACATTCG CACGGGCAAT GGCTATGATG TTCATTCTTT CGAGCCGGGT GATCATGTCA CGCTTTGCGG CGTGAAAATC CCCCATGAGG CAAAGCTCAA CGGTCACTCC GATGCCGATG TGGCCCTTCA TGCGCTGACC GACGCCCTGC TCGCCACGCG CGGTGCGGGT GATATTGGCA CACATTTTCC GCCATCCGAT TCGCAATGGA AAGGAGCTGC CTCACGCATC TTCATCGAAC ATGCGGCCAA AATCGTCCGC GAGGCGGGAG GCCGGATCGC CAATGTGGAC GTGACACTCA TCAGTGAAGC GCCGAAGATC GGCCCCCATC GCGCCGCCAT GACACAAGCA CTCTGCGACA TGCTGGGCAT CGCCGCCGAC CGGGTTTCCA TCAAGGCTAC GACGAACGAA AAGCTTGGCT TTGTCGGCCG TCGCGAAGGC ATTGCAGCCA TCGCAACCGC AACCGTCATC TACCCCGGAG AAGTACCGGA A

Details for BrsuA.00168.a.B1.GE36766

HARVESTED ON:	3/27/2013
SEQUENCED ON:	3/30/2013
EXPECTED MW:	49kDa
OBSERVED MW:	49kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	22
PERCENT COVERAGE:	64

Validated AA Sequence

MCANITRTAR GEQGVGQRMK GHIGIGVTVE LCLMGDFHAA KRDMITRLER MNIIXIARAN VGKYGMVLPQ ALVCHFNILC PGELCIIRRT FDNANSNFLP FGNGGIVGKV GAVDCEGFLM CVQDRRIGKS LRRLRREQAG TGNGGLYHAV CRRPFQRVGD GEGGKHTIIR GEVCNDAFQQ ILTDERTHAI MDQHILRRFV LQGQKTEAGR FLSGGATGNE DDVLMLLRHS APIKRMIVGM DDDGDCADER AIGKSAQSAG KHGLCANEPI LLRPLGALPR PLAATGRHDD CCCL

Validated NT Sequence

cggttgcggt tgcgatggct gcaatgcctt cgcgacggcc gacaaagcca agcttttcgt tcgtcgtagc cttgatggaa acccggtcgg cggcgatgcc cagcatgtcg cagagtgctt gtgtcatggc ggcgcgatgg gggccgatct tcggcgcttc actgatgagt gtcacgtcca cattggcgat ccggcctccc gcctcgcgga cgattttggc cgcatgttcg atgaagatgc gtgaggcagc tcctttccat tgcgaatcgg atggcggaaa atgtgtgcca atatcacccg caccgcgcgt ggcgagcagg gcgtcggtca gcgcatgaag ggccacatcg gcatcggagt gaccgttgag ctttgcctca tgggggattt tcacgccgca aagcgtgaca tgatcacccg gctcgaaaga atgaacatca tanccattgc ccgtgcgaat gtcgggaaat acggcatggt cctgccgcag gcgcttgtct gccatttcaa tatcctttgc ccaggtgagc tttgtattat ccgccgaacc ttcgataatg cgaacagcaa tttcctgcca ttcggcaatg gcggcatcgt cggtaaagtc ggtgcggttg attgcgaagg ctttctcatg tgcgtccaag atcggcgcat agggaaaagc ttgcggcgtc tgcgccgcga acaagccggt acgggaaatg gtggtctcta ccatgccgtc tgccgccgcc cgtttcaacg tgtcggggac ggcgagggcg ggaagcacac cattatcagg ggtgaggttt gcaatgatgc gttccagcaa atcctgaccg atgaaaggac gcacgccatc atggatcaac acatattgcg gcgcttcgtc cttcaaggcc agaagaccga ggcgggtcga ttcctgtcgg gtggggccac cggtaacgag gatgacgttc tcatgcttct ccggcacagc gcgccgataa agcgcatgat cgtcgggatg gatgacgacg gcgattgtgc cgatgagcgg gcaatcggta aaagcgcgca aagtgcgggc aagcacggcc tctgcgccaa tgagccgata ttgcttcggc ccctcggcgc tttgccccgc ccgctcgccg cgaccggccg ccacgatgac tgctgctgcc tttgaaatga ctgtaacctc tgccaaagat tgtgcctgca agccgaataa ggccttgcga cgacaaatgc cagacggcaa ttgatttatc gtgcgttcag gctgcttttc cttgcgttcg gaaagcgggg tgattaaact gtatgc

Expected Protein Sequence

MAHHHHHHMK SAYSLITPLS ERKEKQPERT INQLPSGICR RKALFGLQAQ SLAEVTVISK AAAVIVAAGR GERAGQSAEG PKQYRLIGAE AVLARTLRAF TDCPLIGTIA VVIHPDDHAL YRRAVPEKHE NVILVTGGPT RQESTRLGLL ALKDEAPQYV LIHDGVRPFI GQDLLERIIA NLTPDNGVLP ALAVPDTLKR AAADGMVETT ISRTGLFAAQ TPQAFPYAPI LDAHEKAFAI NRTDFTDDAA IAEWQEIAVR IIEGSADNTK LTWAKDIEMA DKRLRQDHAV FPDIRTGNGY DVHSFEPGDH VTLCGVKIPH EAKLNGHSDA DVALHALTDA LLATRGAGDI GTHFPPSDSQ WKGAASRIFI EHAAKIVREA GGRIANVDVT LISEAPKIGP HRAAMTQALC DMLGIAADRV SIKATTNEKL GFVGRREGIA AIATATVIYP GEVPE

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaat ccgcatacag tttaatcacc ccgctttccg aacgcaagga aaagcagcct gaacgcacga taaatcaatt gccgtctggc atttgtcgtc gcaaggcctt attcggcttg caggcacaat ctttggcaga ggttacagtc atttcaaagg cagcagcagt catcgtggcg gccggtcgcg gcgagcgggc ggggcaaagc gccgaggggc cgaagcaata tcggctcatt ggcgcagagg ccgtgcttgc ccgcactttg cgcgctttta ccgattgccc gctcatcggc acaatcgccg tcgtcatcca tcccgacgat catgcgcttt atcggcgcgc tgtgccggag aagcatgaga acgtcatcct cgttaccggt ggccccaccc gacaggaatc gacccgcctc ggtcttctgg ccttgaagga cgaagcgccg caatatgtgt tgatccatga tggcgtgcgt cctttcatcg gtcaggattt gctggaacgc atcattgcaa acctcacccc tgataatggt gtgcttcccg ccctcgccgt ccccgacacg ttgaaacggg cggcggcaga cggcatggta gagaccacca tttcccgtac cggcttgttc gcggcgcaga cgccgcaagc ttttccctat gcgccgatct tggacgcaca tgagaaagcc ttcgcaatca accgcaccga ctttaccgac gatgccgcca ttgccgaatg gcaggaaatt gctgttcgca ttatcgaagg ttcggcggat aatacaaagc tcacctgggc aaaggatatt gaaatggcag acaagcgcct gcggcaggac catgccgtat ttcccgacat tcgcacgggc aatggctatg atgttcattc tttcgagccg ggtgatcatg tcacgctttg cggcgtgaaa atcccccatg aggcaaagct caacggtcac tccgatgccg atgtggccct tcatgcgctg accgacgccc tgctcgccac gcgcggtgcg ggtgatattg gcacacattt tccgccatcc gattcgcaat ggaaaggagc tgcctcacgc atcttcatcg aacatgcggc caaaatcgtc cgcgaggcgg gaggccggat cgccaatgtg gacgtgacac tcatcagtga agcgccgaag atcggccccc atcgcgccgc catgacacaa gcactctgcg acatgctggg catcgccgcc gaccgggttt ccatcaaggc tacgacgaac gaaaagcttg gctttgtcgg ccgtcgcgaa ggcattgcag ccatcgcaac cgcaaccgtc atctaccccg gagaagtacc ggaatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat

Details for BrsuA.00168.a.B1.PS01895

PURIFICATION DATe:	9/4/2013
CONCENTRATION:	20.49mg/ml
OBSERVED MW:	47kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	2
VIAL VOLUME:	110µl
PERCENT IDENTITY:	22
PERCENT COVERAGE:	64

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAAAT CCGCATACAG TTTAATCACC CCGCTTTCCG AACGCAAGGA AAAGCAGCCT GAACGCACGA TAAATCAATT GCCGTCTGGC ATTTGTCGTC GCAAGGCCTT ATTCGGCTTG CAGGCACAAT CTTTGGCAGA GGTTACAGTC ATTTCAAAGG CAGCAGCAGT CATCGTGGCG GCCGGTCGCG GCGAGCGGGC GGGGCAAAGC GCCGAGGGGC CGAAGCAATA TCGGCTCATT GGCGCAGAGG CCGTGCTTGC CCGCACTTTG CGCGCTTTTA CCGATTGCCC GCTCATCGGC ACAATCGCCG TCGTCATCCA TCCCGACGAT CATGCGCTTT ATCGGCGCGC TGTGCCGGAG AAGCATGAGA ACGTCATCCT CGTTACCGGT GGCCCCACCC GACAGGAATC GACCCGCCTC GGTCTTCTGG CCTTGAAGGA CGAAGCGCCG CAATATGTGT TGATCCATGA TGGCGTGCGT CCTTTCATCG GTCAGGATTT GCTGGAACGC ATCATTGCAA ACCTCACCCC TGATAATGGT GTGCTTCCCG CCCTCGCCGT CCCCGACACG TTGAAACGGG CGGCGGCAGA CGGCATGGTA GAGACCACCA TTTCCCGTAC CGGCTTGTTC GCGGCGCAGA CGCCGCAAGC TTTTCCCTAT GCGCCGATCT TGGACGCACA TGAGAAAGCC TTCGCAATCA ACCGCACCGA CTTTACCGAC GATGCCGCCA TTGCCGAATG GCAGGAAATT GCTGTTCGCA TTATCGAAGG TTCGGCGGAT AATACAAAGC TCACCTGGGC AAAGGATATT GAAATGGCAG ACAAGCGCCT GCGGCAGGAC CATGCCGTAT TTCCCGACAT TCGCACGGGC AATGGCTATG ATGTTCATTC TTTCGAGCCG GGTGATCATG TCACGCTTTG CGGCGTGAAA ATCCCCCATG AGGCAAAGCT CAACGGTCAC TCCGATGCCG ATGTGGCCCT TCATGCGCTG ACCGACGCCC TGCTCGCCAC GCGCGGTGCG GGTGATATTG GCACACATTT TCCGCCATCC GATTCGCAAT GGAAAGGAGC TGCCTCACGC ATCTTCATCG AACATGCGGC CAAAATCGTC CGCGAGGCGG GAGGCCGGAT CGCCAATGTG GACGTGACAC TCATCAGTGA AGCGCCGAAG ATCGGCCCCC ATCGCGCCGC CATGACACAA GCACTCTGCG ACATGCTGGG CATCGCCGCC GACCGGGTTT CCATCAAGGC TACGACGAAC GAAAAGCTTG GCTTTGTCGG CCGTCGCGAA GGCATTGCAG CCATCGCAAC CGCAACCGTC ATCTACCCCG GAGAAGTACC GGAATGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT