SSGCID: Target Page

BrsuA.00107.c

RND efflux system, membrane fusion protein CmeA

CENTER ID:	BrsuA.00107.c
ORGANISM:	Brucella suis 1330
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrsuA.00107.c.B1.GE36762	Full length( BrsuA.00107.c )		1	411

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	PATRIC_Locus_Tag:VBIBruSui107850_2550
BV-BRC:	fig\|204722.5.peg.2550
RefSeq:	NP_699530.1
RefSeq:	YP_005614040.1
UniProt:	Q8FWV8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MVAFWTCRNA WFQHLPFAKR GDENAPSGPR RLRPWFLVLA LGLAACSEDK SAPQQAAPLP PIPVGVIKIT ERPTHPQLSF VGRVEATDSV DLIARVDGFL DKRTFTEGQA VKTGDLLFVL QKDALQAALD AAQANLAKAQ ADADNLKLQT ERARSLYKQK TVSQAMLDDR VAAEKQALAV VQQAQASLEQ AQINLGYTDI RAPFSGRIGM ANFSVGALVG PSSGPLATIV SQDPIYVTFP VSDKTILDLT EGGRTATDRS NVAVSLTLSN GMTYPQTGAI DFTGIKINPN TDTLMVRAQF PNPNNVLIDG QYVQVTAASK HPVEALLVPQ KAIMTDQSGN YVLAVGEDNK VIQRQITQGS TFGSNVVVKS GLAVGDQVVV DGLQRIRPGQ KVDPQIVDAT TPAQKAMSVG N

NT Sequence

ATGGTTGCCT TTTGGACGTG CCGAAACGCT TGGTTCCAAC ACCTTCCTTT TGCGAAGCGC GGCGACGAAA ATGCGCCATC TGGACCACGC CGGCTGCGCC CCTGGTTCCT CGTTCTGGCT CTGGGGCTTG CAGCGTGCAG CGAAGACAAG TCAGCTCCGC AGCAAGCCGC TCCCCTGCCC CCGATACCTG TCGGCGTCAT CAAGATAACA GAGCGCCCCA CCCATCCCCA GCTCTCCTTC GTCGGACGTG TCGAGGCAAC CGATAGCGTC GATCTGATAG CCCGCGTCGA TGGCTTTCTC GACAAGCGCA CCTTCACCGA AGGTCAGGCC GTAAAGACCG GGGATTTGCT GTTTGTCCTG CAAAAGGATG CGCTTCAGGC GGCGCTGGAT GCGGCGCAGG CCAATCTTGC AAAAGCGCAG GCCGATGCCG ACAACCTGAA ACTCCAGACC GAGCGCGCCC GCTCGCTCTA CAAGCAGAAG ACCGTTTCGC AAGCCATGCT TGATGATCGT GTGGCAGCGG AAAAGCAGGC TTTGGCCGTC GTGCAGCAGG CGCAGGCTTC GCTGGAACAG GCGCAAATCA ATCTCGGCTA TACCGATATA CGCGCGCCTT TTTCCGGACG CATCGGCATG GCCAATTTCA GCGTCGGCGC CCTTGTCGGG CCGTCCTCAG GCCCTCTGGC AACGATCGTC AGCCAGGACC CGATTTACGT CACCTTCCCC GTCAGCGACA AGACCATTCT CGATTTAACC GAAGGCGGGA GAACGGCCAC AGACCGCAGC AATGTAGCCG TCAGCCTGAC GCTCTCCAAC GGCATGACAT ATCCGCAAAC GGGGGCCATC GACTTTACCG GGATCAAGAT TAATCCCAAT ACCGATACGC TGATGGTCCG GGCACAGTTT CCCAATCCGA ACAATGTCCT GATCGACGGG CAGTATGTTC AGGTAACGGC GGCGAGCAAA CACCCCGTTG AAGCCCTGCT CGTCCCGCAA AAGGCAATCA TGACCGACCA GAGCGGCAAT TACGTTCTGG CCGTCGGTGA AGATAACAAG GTCATCCAGC GCCAGATCAC GCAAGGTTCG ACCTTTGGCA GCAATGTTGT GGTCAAGTCC GGCCTCGCGG TGGGCGATCA GGTCGTGGTC GATGGCCTCC AGCGCATCCG GCCCGGCCAG AAGGTCGATC CGCAGATCGT CGATGCAACC ACGCCCGCCC AGAAAGCCAT GTCGGTGGGG AAT

Details for BrsuA.00107.c.B1.GE36762

HARVESTED ON:	3/27/2013
SEQUENCED ON:	3/30/2013
EXPECTED MW:	45kDa
OBSERVED MW:	42kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	83
PERCENT COVERAGE:	64

Validated AA Sequence

Validated NT Sequence

tttnnnnnnn ttaagaagga ganataccat ggctcaccac caccaccacc atatggttgc cttttggacg tgccgaaacg cttggttcca acaccttcct tttgcgaagc gcggcgacga aaatgcgcca tctggaccac gccggctgcg cccctggttc ctcgttctgg ctctggggct tgcagcgtgc agcgaagaca agtcagctcc gcagcaagcc gctcccctgc ccccgatacc tgtcggcgtc atcaagataa cagagcgccc cacccatccc cagctctcct tcgtcggacg tgtcgaggca accgatagcg tcgatctgat agcccgcgtc gatggctttc tcgacaagcg caccttcacc gaaggtcagg ccgtaaagac cggggatttg ctgtttgtcc tgcaaaagga tgcgcttcag gcggcgctgg atgcggcgca ggccaatctt gcaaaagcgc aggccgatgc cgacaacctg aaactccaga ccgagcgcgc ccgctcgctc tacaagcaga agaccgtttc gcaagccatg cttgatgatc gtgtggcagc ggaaaagcag gctttggccg tcgtgcagca ggcgcangct tcgctggaac aggcgcaaat caatctcggc tataccgata tacgcgcgcc tttttccgga cgcatcggca tngncnaatt tcagcgtcng cgcccttgtc ngggccgtcc tcnggccctc tggcaacgat cgtcagccag gacccgattt acgtcnncnt tccccgtcag cganaganca ttctngattt nnncnaannn nggnnnacgg cnnnnnaccg cancnntgta gcngtcannn tgannnnnnn cnncngnatg annnatccnc annggggnna tcganttnnn nnnncnnnan nnnnccnnnn cnnnncncta ngnnngnnnn nnntncnnnn nannnnnnnn nnnnnncnan nnnanntnng nnangnnnnn nnnannnnnn gncnnannnn nnngnc

Expected Protein Sequence

MAHHHHHHMV AFWTCRNAWF QHLPFAKRGD ENAPSGPRRL RPWFLVLALG LAACSEDKSA PQQAAPLPPI PVGVIKITER PTHPQLSFVG RVEATDSVDL IARVDGFLDK RTFTEGQAVK TGDLLFVLQK DALQAALDAA QANLAKAQAD ADNLKLQTER ARSLYKQKTV SQAMLDDRVA AEKQALAVVQ QAQASLEQAQ INLGYTDIRA PFSGRIGMAN FSVGALVGPS SGPLATIVSQ DPIYVTFPVS DKTILDLTEG GRTATDRSNV AVSLTLSNGM TYPQTGAIDF TGIKINPNTD TLMVRAQFPN PNNVLIDGQY VQVTAASKHP VEALLVPQKA IMTDQSGNYV LAVGEDNKVI QRQITQGSTF GSNVVVKSGL AVGDQVVVDG LQRIRPGQKV DPQIVDATTP AQKAMSVGN

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggttg ccttttggac gtgccgaaac gcttggttcc aacaccttcc ttttgcgaag cgcggcgacg aaaatgcgcc atctggacca cgccggctgc gcccctggtt cctcgttctg gctctggggc ttgcagcgtg cagcgaagac aagtcagctc cgcagcaagc cgctcccctg cccccgatac ctgtcggcgt catcaagata acagagcgcc ccacccatcc ccagctctcc ttcgtcggac gtgtcgaggc aaccgatagc gtcgatctga tagcccgcgt cgatggcttt ctcgacaagc gcaccttcac cgaaggtcag gccgtaaaga ccggggattt gctgtttgtc ctgcaaaagg atgcgcttca ggcggcgctg gatgcggcgc aggccaatct tgcaaaagcg caggccgatg ccgacaacct gaaactccag accgagcgcg cccgctcgct ctacaagcag aagaccgttt cgcaagccat gcttgatgat cgtgtggcag cggaaaagca ggctttggcc gtcgtgcagc aggcgcaggc ttcgctggaa caggcgcaaa tcaatctcgg ctataccgat atacgcgcgc ctttttccgg acgcatcggc atggccaatt tcagcgtcgg cgcccttgtc gggccgtcct caggccctct ggcaacgatc gtcagccagg acccgattta cgtcaccttc cccgtcagcg acaagaccat tctcgattta accgaaggcg ggagaacggc cacagaccgc agcaatgtag ccgtcagcct gacgctctcc aacggcatga catatccgca aacgggggcc atcgacttta ccgggatcaa gattaatccc aataccgata cgctgatggt ccgggcacag tttcccaatc cgaacaatgt cctgatcgac gggcagtatg ttcaggtaac ggcggcgagc aaacaccccg ttgaagccct gctcgtcccg caaaaggcaa tcatgaccga ccagagcggc aattacgttc tggccgtcgg tgaagataac aaggtcatcc agcgccagat cacgcaaggt tcgacctttg gcagcaatgt tgtggtcaag tccggcctcg cggtgggcga tcaggtcgtg gtcgatggcc tccagcgcat ccggcccggc cagaaggtcg atccgcagat cgtcgatgca accacgcccg cccagaaagc catgtcggtg gggaattgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t