BrsuA.00085.a

GDP-L-fucose synthetase (EC 1.1.1.271)

CENTER ID: BrsuA.00085.a
ORGANISM: Brucella suis 1330
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrsuA.00085.a.B1.GE36761 Full length( BrsuA.00085.a ) 1 356
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruSui107850_2633
BV-BRC: fig|204722.5.peg.2633
RefSeq: NP_699610.1
RefSeq: YP_005614120.1
UniProt: A0A0H3G687

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQDKTALIVG VTGQDGAYLS ELLLGKGYRV HGLKRRSSSF NTARIDHLYQ DPHEEDIRFR LHFGDLTDAT NLCRVIQEVQ PDEIYNLGAQ SHVQVSFETP EYTANADALG TLRLLESMRI LGLGKSCRFY QASTSELFGN SSHHAQNEQT PFAPRSPYAT AKLYAYWTTV NYRDAYGFHA SNGILFNHES PLRGETFVTR KITRAVAAIE RGLQDRLRLG NLEARRDWGH ARDYVEGMWR ILQEDTPDDY VLATGETHTV REFIEHAFKA VDKQIVWHGD GVDEVGIDRK TGNCLIEIDP RYFRPNEVDF LMGDASKAKD RLGWQHTTGF ENLVAEMVDW DLRNIMREVG RNDFYG
NT Sequence
TTGCAGGATA AAACAGCTCT CATCGTCGGT GTAACCGGGC AGGATGGCGC CTATCTAAGC GAGCTTCTGC TGGGCAAGGG CTATCGGGTG CATGGCCTGA AAAGGCGGTC ATCGTCATTC AATACGGCGC GTATCGACCA TCTTTATCAA GACCCGCATG AGGAAGACAT CCGCTTTCGC CTGCATTTCG GCGACCTGAC AGACGCAACC AATCTGTGCC GCGTCATCCA GGAAGTGCAG CCTGACGAGA TTTATAATCT CGGCGCCCAG AGCCATGTGC AGGTGAGTTT TGAAACGCCG GAATATACGG CCAATGCCGA TGCGCTCGGC ACACTGCGCC TGCTTGAATC CATGCGCATC CTGGGTCTCG GCAAAAGCTG CCGTTTCTAT CAGGCTTCCA CTTCGGAGCT TTTCGGCAAT AGTTCCCACC ATGCGCAAAA CGAGCAGACG CCTTTCGCTC CGCGCAGCCC ATATGCAACC GCCAAGCTTT ATGCTTACTG GACAACGGTG AACTATCGCG ATGCCTATGG TTTCCACGCC TCGAACGGCA TCTTGTTCAA TCATGAAAGC CCCTTGCGGG GCGAAACCTT CGTCACCCGC AAGATCACGC GCGCAGTTGC AGCTATCGAA CGCGGGCTAC AGGACAGGCT GCGGCTTGGC AATCTTGAGG CCCGCCGCGA CTGGGGCCAT GCGCGCGATT ATGTCGAAGG CATGTGGCGC ATTCTACAGG AAGACACACC GGACGATTAT GTGCTCGCCA CGGGTGAAAC GCATACGGTG CGTGAATTCA TCGAACACGC CTTCAAGGCC GTTGACAAGC AGATCGTATG GCATGGCGAT GGCGTGGACG AGGTCGGCAT CGACCGCAAG ACGGGCAATT GCCTGATCGA GATCGACCCG CGCTATTTCC GCCCCAACGA AGTGGATTTC CTTATGGGCG ATGCTTCCAA GGCAAAAGAC CGTCTGGGCT GGCAACATAC GACCGGCTTT GAAAATCTCG TGGCTGAAAT GGTGGATTGG GATCTGCGCA ACATCATGCG GGAGGTAGGA CGCAATGATT TCTACGGC
Details for BrsuA.00085.a.B1.GE36761
HARVESTED ON: 3/27/2013
SEQUENCED ON: 3/30/2013
EXPECTED MW: 42kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 95
Validated AA Sequence
MLQDKTALIV GVTGQDGAYL SELLLGKGYR VHGLKRRSSS FNTARIDHLY QDPHEEDIRF RLHFGDLTDA TNLCRVIQEV QPDEIYNLGA QSHVQVSFET PEYTANADAL GTLRLLESMR ILGLGKSCRF YQASTSELFG NSSHHAQNEQ TPFAPRSPYA TAKLYAYWTT VNYRDAYGFH ASNGILFNHE SPLRGETFVT RKITRAVAAI ERGLQDRLRL GNLEARRDWG HARDYVEGMW RILQEDTPDD YVLATGETHT VREFIEHAFK AVDKQIVWHG DGVDEVGIDR KTGNCLIEID PRYFRPNEVD FLMGDASKAK DRLGWQHTTG FENLVAEMVD WDLRNIM
Validated NT Sequence
cgcatgatgt tgcgcagatc ccaatccacc atttcagcca cgagattttc aaagccggtc gtatgttgcc agcccagacg gtcttttgcc ttggaagcat cgcccataag gaaatccact tcgttggggc ggaaatagcg cgggtcgatc tcgatcaggc aattgcccgt cttgcggtcg atgccgacct cgtccacgcc atcgccatgc catacgatct gcttgtcaac ggccttgaag gcgtgttcga tgaattcacg caccgtatgc gtttcacccg tggcgagcac ataatcgtcc ggtgtgtctt cctgtagaat gcgccacatg ccttcgacat aatcgcgcgc atggccccag tcgcggcggg cctcaagatt gccaagccgc agcctgtcct gtagcccgcg ttcgatagct gcaactgcgc gcgtgatctt gcgggtgacg aaggtttcgc cccgcaaggg gctttcatga ttgaacaaga tgccgttcga ggcgtggaaa ccataggcat cgcgatagtt caccgttgtc cagtaagcat aaagcttggc ggttgcatat gggctgcgcg gagcgaaagg cgtctgctcg ttttgcgcat ggtgggaact attgccgaaa agctccgaag tggaagcctg atagaaacgg cagcttttgc cgagacccag gatgcgcatg gattcaagca ggcgcagtgt gccgagcgca tcggcattgg ccgtatattc cggcgtttca aaactcacct gcacatggct ctgggcgccg agattataaa tctcgtcagg ctgcacttcc tggatgacgc ggcacagatt ggttgcgtct gtcaggtcgc cgaaatgcag gcgaaagcgg atgtcttcct catgcgggtc ttgataaaga tggtcgatac gcgccgtatt gaatgacgat gaccgccttt tcaggccatg cacccgatag cccttgccca gcagaagctc gcttagatag gcgccatcct gcccggttac accgacgatg agagctgttt tatcctgcaa catatggtgg tg
Expected Protein Sequence
MAHHHHHHMQ DKTALIVGVT GQDGAYLSEL LLGKGYRVHG LKRRSSSFNT ARIDHLYQDP HEEDIRFRLH FGDLTDATNL CRVIQEVQPD EIYNLGAQSH VQVSFETPEY TANADALGTL RLLESMRILG LGKSCRFYQA STSELFGNSS HHAQNEQTPF APRSPYATAK LYAYWTTVNY RDAYGFHASN GILFNHESPL RGETFVTRKI TRAVAAIERG LQDRLRLGNL EARRDWGHAR DYVEGMWRIL QEDTPDDYVL ATGETHTVRE FIEHAFKAVD KQIVWHGDGV DEVGIDRKTG NCLIEIDPRY FRPNEVDFLM GDASKAKDRL GWQHTTGFEN LVAEMVDWDL RNIMREVGRN DFYG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttgcagg ataaaacagc tctcatcgtc ggtgtaaccg ggcaggatgg cgcctatcta agcgagcttc tgctgggcaa gggctatcgg gtgcatggcc tgaaaaggcg gtcatcgtca ttcaatacgg cgcgtatcga ccatctttat caagacccgc atgaggaaga catccgcttt cgcctgcatt tcggcgacct gacagacgca accaatctgt gccgcgtcat ccaggaagtg cagcctgacg agatttataa tctcggcgcc cagagccatg tgcaggtgag ttttgaaacg ccggaatata cggccaatgc cgatgcgctc ggcacactgc gcctgcttga atccatgcgc atcctgggtc tcggcaaaag ctgccgtttc tatcaggctt ccacttcgga gcttttcggc aatagttccc accatgcgca aaacgagcag acgcctttcg ctccgcgcag cccatatgca accgccaagc tttatgctta ctggacaacg gtgaactatc gcgatgccta tggtttccac gcctcgaacg gcatcttgtt caatcatgaa agccccttgc ggggcgaaac cttcgtcacc cgcaagatca cgcgcgcagt tgcagctatc gaacgcgggc tacaggacag gctgcggctt ggcaatcttg aggcccgccg cgactggggc catgcgcgcg attatgtcga aggcatgtgg cgcattctac aggaagacac accggacgat tatgtgctcg ccacgggtga aacgcatacg gtgcgtgaat tcatcgaaca cgccttcaag gccgttgaca agcagatcgt atggcatggc gatggcgtgg acgaggtcgg catcgaccgc aagacgggca attgcctgat cgagatcgac ccgcgctatt tccgccccaa cgaagtggat ttccttatgg gcgatgcttc caaggcaaaa gaccgtctgg gctggcaaca tacgaccggc tttgaaaatc tcgtggctga aatggtggat tgggatctgc gcaacatcat gcgggaggta ggacgcaatg atttctacgg ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat