BrsuA.00057.e

Putrescine transport ATP-binding protein PotA (TC 3.A.1.11.1)

CENTER ID: BrsuA.00057.e
ORGANISM: Brucella suis 1330
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrsuA.00057.e.B1.GE36759 Full length( BrsuA.00057.e ) 1 245
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruSui107850_3334

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGISIEGVTK KYGSFTAVDG LDMQIAPGEF LALLGPSGSG KSTILMLLAG FEHPDNGRIL FGNNDYTRVP PHRRNIGMVF QHYTLFPNMS VLDNVAFPLK TRGMERSQRH RIAREALARV RLADFADRSP KQLSGGQQQR VALARSIVYS PRVLLMDEPL SALDKNLREE MQIEIKRLHT ELGMTIVFVT HDQGEALTMA DRVAILQGGH IQQIAGAREL YNRPANLFSA RFIGEMNLIP VDWDG
NT Sequence
TTGGGTATCA GCATTGAAGG CGTCACCAAG AAATATGGCA GCTTCACCGC AGTCGACGGG CTGGATATGC AGATTGCGCC TGGCGAATTT CTGGCGTTGC TGGGGCCTTC CGGATCGGGA AAGTCCACCA TCCTGATGCT GCTGGCGGGG TTCGAGCACC CGGATAATGG CCGCATTCTC TTCGGCAATA ATGATTATAC CCGTGTTCCG CCGCACAGGC GCAATATTGG CATGGTATTC CAGCATTACA CCCTGTTTCC CAATATGAGC GTGCTCGATA ATGTGGCGTT CCCGCTCAAG ACGCGGGGCA TGGAGCGCAG CCAGCGCCAC AGGATCGCGC GTGAAGCGCT GGCGCGCGTG CGGCTTGCCG ATTTTGCGGA TCGCAGTCCC AAGCAATTGT CCGGCGGGCA GCAGCAACGC GTCGCGCTGG CACGCTCCAT TGTCTATTCG CCGCGCGTGC TCCTGATGGA TGAGCCGCTT TCGGCCCTGG ACAAGAACCT GCGCGAAGAG ATGCAGATCG AGATCAAGCG CTTGCATACC GAGCTTGGCA TGACGATCGT ATTCGTGACC CATGACCAGG GCGAGGCCCT CACCATGGCG GACAGGGTGG CGATTCTGCA AGGTGGCCAT ATTCAGCAGA TCGCAGGCGC CCGCGAGCTT TACAATAGAC CGGCAAACCT GTTCTCCGCC AGGTTTATTG GCGAGATGAA TCTGATTCCG GTCGACTGGG ATGGC
Details for BrsuA.00057.e.B1.GE36759
HARVESTED ON: 3/27/2013
SEQUENCED ON: 3/30/2013
EXPECTED MW: 28kDa
OBSERVED MW: 27kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHML GISIEGVTKK YGSFTAVDGL DMQIAPGEFL ALLGPSGSGK STILMLLAGF EHPDNGRILF GNNDYTRVPP HRRNIGMVFQ HYTLFPNMSV LDNVAFPLKT RGMERSQRHR IAREALARVR LADFADRSPK QLSGGQQQRV ALARSIVYSP RVLLMDEPLS ALDKNLREEM QIEIKRLHTE LGMTIVFVTH DQGEALTMAD RVAILQGGHI QQIAGARELY NRPANLFSAR FIGEMNLIPV DWDG
Validated NT Sequence
annnnnnttn nnnnnnttaa gaaggagana taccatggct caccaccacc accaccatat gttgggtatc agcattgaag gcgtcaccaa gaaatatggc agcttcaccg cagtcgacgg gctggatatg cagattgcgc ctggcgaatt tctggcgttg ctggggcctt ccggatcggg aaagtccacc atcctgatgc tgctggcggg gttcgagcac ccggataatg gccgcattct cttcggcaat aatgattata cccgtgttcc gccgcacagg cgcaatattg gcatggtatt ccagcattac accctgtttc ccaatatgag cgtgctcgat aatgtggcgt tcccgctcaa gacgcggggc atggagcgca gccagcgcca caggatcgcg cgtgaagcgc tggcgcgcgt gcggcttgcc gattttgcgg atcgcagtcc caagcaattg tccggcgggc agcagcaacg cgtcgcgctg gcacgctcca ttgtctattc gccgcgcgtg ctcctgatgg atgagccgct ttcggccctg gacaagaacc tgcgcgaaga gatgcagatc gagatcaagc gcttgcatac cgagcttggc atgacgatcg tattcgtgac ccatgaccag ggcgaggccc tcaccatggc ggacagggtg gcgattctgc aaggtggcca tattcagcag atcgcaggcg cccgcgagct ttacaataga ccggcaaacc tgttctccgc caggtttatt ggcgagatga atctgattcc ggtcgactgg gatggctaag tgagtaagat aggatccggc tgnnnnnnnn ccccnnannn nannnnnnnn nnnnnnncnn nnnnnnngnc ntcccnnnnc nnntntnnnn nnnntnttnc cnnntnnncc cgccncnnnn nnntcnnntn nntncnnnnn cnnnnnnnnc nnntctccnc nnnnccctnn ncnnnnnctn tnnntcntna nntnnnnnnn ccncnncnnn nnncnnctcc cnnnntctcc ncnnnnnncn nnnnnnnnnn nncncnnccn cncccnnncc nncncccnnn nnncntcnnn nncnnncnnn nnnnnnnnnn nnnnnnnnnn nntnnnnnnn ncccncnnnn nnnnnnnnnn ccnnnnnnnn tnnnnnnnng nnnnncnnnn nnnnnnnnnn nnnnnnnnnn nttnncnc
Expected Protein Sequence
MAHHHHHHMG ISIEGVTKKY GSFTAVDGLD MQIAPGEFLA LLGPSGSGKS TILMLLAGFE HPDNGRILFG NNDYTRVPPH RRNIGMVFQH YTLFPNMSVL DNVAFPLKTR GMERSQRHRI AREALARVRL ADFADRSPKQ LSGGQQQRVA LARSIVYSPR VLLMDEPLSA LDKNLREEMQ IEIKRLHTEL GMTIVFVTHD QGEALTMADR VAILQGGHIQ QIAGARELYN RPANLFSARF IGEMNLIPVD WDG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttgggta tcagcattga aggcgtcacc aagaaatatg gcagcttcac cgcagtcgac gggctggata tgcagattgc gcctggcgaa tttctggcgt tgctggggcc ttccggatcg ggaaagtcca ccatcctgat gctgctggcg gggttcgagc acccggataa tggccgcatt ctcttcggca ataatgatta tacccgtgtt ccgccgcaca ggcgcaatat tggcatggta ttccagcatt acaccctgtt tcccaatatg agcgtgctcg ataatgtggc gttcccgctc aagacgcggg gcatggagcg cagccagcgc cacaggatcg cgcgtgaagc gctggcgcgc gtgcggcttg ccgattttgc ggatcgcagt cccaagcaat tgtccggcgg gcagcagcaa cgcgtcgcgc tggcacgctc cattgtctat tcgccgcgcg tgctcctgat ggatgagccg ctttcggccc tggacaagaa cctgcgcgaa gagatgcaga tcgagatcaa gcgcttgcat accgagcttg gcatgacgat cgtattcgtg acccatgacc agggcgaggc cctcaccatg gcggacaggg tggcgattct gcaaggtggc catattcagc agatcgcagg cgcccgcgag ctttacaata gaccggcaaa cctgttctcc gccaggttta ttggcgagat gaatctgatt ccggtcgact gggatggctg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat