BrsuA.00027.a
Isovaleryl-CoA dehydrogenase (EC 1.3.99.10)
| CENTER ID: | BrsuA.00027.a |
| ORGANISM: | Brucella suis 1330 |
| ASSOCIATED DISEASE: | Brucellosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrsuA.00027.a.B1.GE36752 | Full length( BrsuA.00027.a ) | 1 | 382 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrsuA.00027.a.B1.PS01889 | Full length( BrsuA.00027.a ) | 1 | 382 |
Structures
4O5M
DEPOSITED: 12/19/2013
DETERMINATION: XRay
CLONE: BrsuA.00027.a.B1.GE36752
PROTEIN: BrsuA.00027.a.B1.PS01889
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | PATRIC_Locus_Tag:VBIBruSui107850_0022 |
| BV-BRC: | fig|204722.5.peg.22 |
| RefSeq: | NP_697062.1 |
| RefSeq: | YP_005614881.1 |
| UniProt: | A0A0H3G544 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNFGLGEEIE ALRDTVRRFA ESRIAPLAAE TDRNNQFPMH LWREFGELGV LGITAPEDYG GAGMGYLAHC IAMEEISRAS ASIGLSYGAH SNLCVNQITR NGSPEQRAKY LPKLISGEHV GALAMSEPGA GSDVVSMKLA AEKRGDRYVL NGNKMWITNG PDADVLVVYA KTDLSAGPRG ISAFIIEKGF KGFSTAQKLD KLGMRGSNTC ELVFEDCEVP AENLLGTEGK GVNVLMSGLD YERVVLAGGP LGIMAACLDV VVPYVHERKQ FDQPIGEFQL MQCKLADMYV TFNASRAYVY AVAAACDRGE TTRKDAAGCI LYSAENATQM ALQAIQSLGG NGYINDYPTG RLLRDAKLYE IGAGTSEIRR MLIGRELFQE TR
NT Sequence
ATGAACTTCG GTCTTGGCGA GGAGATCGAA GCCCTTCGTG ACACTGTGCG CCGTTTCGCC GAAAGCCGCA TTGCGCCGCT TGCCGCTGAA ACCGACCGCA ATAATCAATT TCCCATGCAT CTTTGGCGTG AATTTGGTGA GCTTGGCGTA CTCGGCATTA CGGCGCCGGA AGACTATGGT GGCGCAGGCA TGGGCTATCT CGCCCATTGC ATCGCCATGG AGGAGATCAG CCGTGCTTCC GCCTCCATCG GCCTTAGCTA CGGCGCCCAT TCCAATCTCT GCGTCAACCA GATCACCCGC AACGGTTCGC CCGAACAGCG CGCGAAATAT CTGCCGAAAC TCATTTCCGG TGAGCATGTC GGCGCGCTGG CCATGTCTGA GCCGGGCGCG GGGTCCGATG TTGTTTCCAT GAAGCTTGCC GCCGAAAAGC GCGGGGACCG TTACGTCCTC AACGGCAACA AGATGTGGAT CACCAATGGC CCGGATGCCG ATGTGCTGGT GGTCTATGCC AAGACAGACC TGTCGGCTGG CCCGCGCGGG ATCAGCGCCT TTATCATTGA AAAAGGGTTC AAGGGTTTTT CGACCGCGCA AAAGCTCGAC AAACTCGGTA TGCGCGGTTC CAACACCTGC GAACTGGTGT TCGAGGATTG CGAAGTGCCA GCCGAAAACC TGCTCGGAAC CGAGGGCAAG GGCGTCAATG TGCTGATGTC CGGCCTCGAT TATGAGCGCG TGGTGCTGGC GGGTGGGCCG CTCGGCATCA TGGCGGCCTG CCTCGATGTC GTGGTGCCCT ATGTGCATGA GCGCAAGCAG TTCGACCAGC CAATCGGTGA ATTCCAGCTC ATGCAGTGCA AGCTTGCCGA CATGTATGTG ACCTTCAACG CTTCACGCGC TTATGTCTAT GCCGTGGCCG CAGCCTGCGA CCGGGGTGAG ACGACGCGCA AGGATGCGGC GGGCTGCATT CTTTACTCTG CGGAAAATGC CACGCAGATG GCCTTGCAGG CGATCCAGTC TCTCGGCGGC AACGGCTATA TCAACGATTA TCCGACGGGC CGCCTTTTGC GCGATGCCAA GCTTTATGAA ATCGGCGCGG GCACGTCGGA AATCCGGCGT ATGCTGATCG GACGGGAACT CTTTCAGGAG ACCCGT
Details for BrsuA.00027.a.B1.GE36752
| HARVESTED ON: | 3/27/2013 |
| SEQUENCED ON: | 3/30/2013 |
| EXPECTED MW: | 42kDa |
| OBSERVED MW: | 41kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 85 |
Validated AA Sequence
MHLWREFGEL GVLGITAPED YGGAGMGYLA HCIAMEEISR ASASIGLSYG AHSNLCVNQI TRNGSPEQRA KYLPKLISGE HVGALAMSEP GAGSDVVSMK LAAEKRGDRY VLNGNKMWIT NGPDADVLVV YAKTDLSAGP RGISAFIIEK GFKGFSTAQK LDKLGMRGSN TCELVFEDCE VPAENLLGTE GKGVNVLMSG LDYERVVLAG GPLGIMAACL DVVVPYVHER KQFDQPIGEF QLMQCKLADM YVTFNASRAY VYAVAAACDR GETTRKDAAG CILYSAENAT QMALQAIQSL GGNGYINDYP TGRLLRDAKL YEIGAGTSEI RRMLI
Validated NT Sequence
cgatcagcat acgccggatt tccgacgtgc ccgcgccgat ttcataaagc ttggcatcgc gcaaaaggcg gcccgtcgga taatcgttga tatagccgtt gccgccgaga gactggatcg cctgcaaggc catctgcgtg gcattttccg cagagtaaag aatgcagccc gccgcatcct tgcgcgtcgt ctcaccccgg tcgcaggctg cggccacggc atagacataa gcgcgtgaag cgttgaaggt cacatacatg tcggcaagct tgcactgcat gagctggaat tcaccgattg gctggtcgaa ctgcttgcgc tcatgcacat agggcaccac gacatcgagg caggccgcca tgatgccgag cggcccaccc gccagcacca cgcgctcata atcgaggccg gacatcagca cattgacgcc cttgccctcg gttccgagca ggttttcggc tggcacttcg caatcctcga acaccagttc gcaggtgttg gaaccgcgca taccgagttt gtcgagcttt tgcgcggtcg aaaaaccctt gaaccctttt tcaatgataa aggcgctgat cccgcgcggg ccagccgaca ggtctgtctt ggcatagacc accagcacat cggcatccgg gccattggtg atccacatct tgttgccgtt gaggacgtaa cggtccccgc gcttttcggc ggcaagcttc atggaaacaa catcggaccc cgcgcccggc tcagacatgg ccagcgcgcc gacatgctca ccggaaatga gtttcggcag atatttcgcg cgctgttcgg gcgaaccgtt gcgggtgatc tggttgacgc agagattgga atgggcgccg tagctaaggc cgatggaggc ggaagcacgg ctgatctcct ccatggcgat gcaatgggcg agatagccca tgcctgcgcc accatagtct tccggcgccg taatgccgag tacgccaagc tcaccaaatt cacgccaaag atgcatggga aattgattat tgcggtcggt ttcagcggca agcggcgcaa tgcggctttc ggcgaaacgg cgcacagtgt cacgaagggc ttcgatctcc tcgccaagac c
Expected Protein Sequence
MAHHHHHHMN FGLGEEIEAL RDTVRRFAES RIAPLAAETD RNNQFPMHLW REFGELGVLG ITAPEDYGGA GMGYLAHCIA MEEISRASAS IGLSYGAHSN LCVNQITRNG SPEQRAKYLP KLISGEHVGA LAMSEPGAGS DVVSMKLAAE KRGDRYVLNG NKMWITNGPD ADVLVVYAKT DLSAGPRGIS AFIIEKGFKG FSTAQKLDKL GMRGSNTCEL VFEDCEVPAE NLLGTEGKGV NVLMSGLDYE RVVLAGGPLG IMAACLDVVV PYVHERKQFD QPIGEFQLMQ CKLADMYVTF NASRAYVYAV AAACDRGETT RKDAAGCILY SAENATQMAL QAIQSLGGNG YINDYPTGRL LRDAKLYEIG AGTSEIRRML IGRELFQETR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaact tcggtcttgg cgaggagatc gaagcccttc gtgacactgt gcgccgtttc gccgaaagcc gcattgcgcc gcttgccgct gaaaccgacc gcaataatca atttcccatg catctttggc gtgaatttgg tgagcttggc gtactcggca ttacggcgcc ggaagactat ggtggcgcag gcatgggcta tctcgcccat tgcatcgcca tggaggagat cagccgtgct tccgcctcca tcggccttag ctacggcgcc cattccaatc tctgcgtcaa ccagatcacc cgcaacggtt cgcccgaaca gcgcgcgaaa tatctgccga aactcatttc cggtgagcat gtcggcgcgc tggccatgtc tgagccgggc gcggggtccg atgttgtttc catgaagctt gccgccgaaa agcgcgggga ccgttacgtc ctcaacggca acaagatgtg gatcaccaat ggcccggatg ccgatgtgct ggtggtctat gccaagacag acctgtcggc tggcccgcgc gggatcagcg cctttatcat tgaaaaaggg ttcaagggtt tttcgaccgc gcaaaagctc gacaaactcg gtatgcgcgg ttccaacacc tgcgaactgg tgttcgagga ttgcgaagtg ccagccgaaa acctgctcgg aaccgagggc aagggcgtca atgtgctgat gtccggcctc gattatgagc gcgtggtgct ggcgggtggg ccgctcggca tcatggcggc ctgcctcgat gtcgtggtgc cctatgtgca tgagcgcaag cagttcgacc agccaatcgg tgaattccag ctcatgcagt gcaagcttgc cgacatgtat gtgaccttca acgcttcacg cgcttatgtc tatgccgtgg ccgcagcctg cgaccggggt gagacgacgc gcaaggatgc ggcgggctgc attctttact ctgcggaaaa tgccacgcag atggccttgc aggcgatcca gtctctcggc ggcaacggct atatcaacga ttatccgacg ggccgccttt tgcgcgatgc caagctttat gaaatcggcg cgggcacgtc ggaaatccgg cgtatgctga tcggacggga actctttcag gagacccgtt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for BrsuA.00027.a.B1.PS01889
| PURIFICATION DATe: | 8/27/2013 |
| CONCENTRATION: | 52.6mg/ml |
| OBSERVED MW: | 39kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 5 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 85 |
Protocol Notes
notes unavailable
Validated AA Sequence
MHLWREFGEL GVLGITAPED YGGAGMGYLA HCIAMEEISR ASASIGLSYG AHSNLCVNQI TRNGSPEQRA KYLPKLISGE HVGALAMSEP GAGSDVVSMK LAAEKRGDRY VLNGNKMWIT NGPDADVLVV YAKTDLSAGP RGISAFIIEK GFKGFSTAQK LDKLGMRGSN TCELVFEDCE VPAENLLGTE GKGVNVLMSG LDYERVVLAG GPLGIMAACL DVVVPYVHER KQFDQPIGEF QLMQCKLADM YVTFNASRAY VYAVAAACDR GETTRKDAAG CILYSAENAT QMALQAIQSL GGNGYINDYP TGRLLRDAKL YEIGAGTSEI RRMLI
Validated NT Sequence
cgatcagcat acgccggatt tccgacgtgc ccgcgccgat ttcataaagc ttggcatcgc gcaaaaggcg gcccgtcgga taatcgttga tatagccgtt gccgccgaga gactggatcg cctgcaaggc catctgcgtg gcattttccg cagagtaaag aatgcagccc gccgcatcct tgcgcgtcgt ctcaccccgg tcgcaggctg cggccacggc atagacataa gcgcgtgaag cgttgaaggt cacatacatg tcggcaagct tgcactgcat gagctggaat tcaccgattg gctggtcgaa ctgcttgcgc tcatgcacat agggcaccac gacatcgagg caggccgcca tgatgccgag cggcccaccc gccagcacca cgcgctcata atcgaggccg gacatcagca cattgacgcc cttgccctcg gttccgagca ggttttcggc tggcacttcg caatcctcga acaccagttc gcaggtgttg gaaccgcgca taccgagttt gtcgagcttt tgcgcggtcg aaaaaccctt gaaccctttt tcaatgataa aggcgctgat cccgcgcggg ccagccgaca ggtctgtctt ggcatagacc accagcacat cggcatccgg gccattggtg atccacatct tgttgccgtt gaggacgtaa cggtccccgc gcttttcggc ggcaagcttc atggaaacaa catcggaccc cgcgcccggc tcagacatgg ccagcgcgcc gacatgctca ccggaaatga gtttcggcag atatttcgcg cgctgttcgg gcgaaccgtt gcgggtgatc tggttgacgc agagattgga atgggcgccg tagctaaggc cgatggaggc ggaagcacgg ctgatctcct ccatggcgat gcaatgggcg agatagccca tgcctgcgcc accatagtct tccggcgccg taatgccgag tacgccaagc tcaccaaatt cacgccaaag atgcatggga aattgattat tgcggtcggt ttcagcggca agcggcgcaa tgcggctttc ggcgaaacgg cgcacagtgt cacgaagggc ttcgatctcc tcgccaagac c
Expressed Protein Sequence
MAHHHHHHMN FGLGEEIEAL RDTVRRFAES RIAPLAAETD RNNQFPMHLW REFGELGVLG ITAPEDYGGA GMGYLAHCIA MEEISRASAS IGLSYGAHSN LCVNQITRNG SPEQRAKYLP KLISGEHVGA LAMSEPGAGS DVVSMKLAAE KRGDRYVLNG NKMWITNGPD ADVLVVYAKT DLSAGPRGIS AFIIEKGFKG FSTAQKLDKL GMRGSNTCEL VFEDCEVPAE NLLGTEGKGV NVLMSGLDYE RVVLAGGPLG IMAACLDVVV PYVHERKQFD QPIGEFQLMQ CKLADMYVTF NASRAYVYAV AAACDRGETT RKDAAGCILY SAENATQMAL QAIQSLGGNG YINDYPTGRL LRDAKLYEIG AGTSEIRRML IGRELFQETR
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAACT TCGGTCTTGG CGAGGAGATC GAAGCCCTTC GTGACACTGT GCGCCGTTTC GCCGAAAGCC GCATTGCGCC GCTTGCCGCT GAAACCGACC GCAATAATCA ATTTCCCATG CATCTTTGGC GTGAATTTGG TGAGCTTGGC GTACTCGGCA TTACGGCGCC GGAAGACTAT GGTGGCGCAG GCATGGGCTA TCTCGCCCAT TGCATCGCCA TGGAGGAGAT CAGCCGTGCT TCCGCCTCCA TCGGCCTTAG CTACGGCGCC CATTCCAATC TCTGCGTCAA CCAGATCACC CGCAACGGTT CGCCCGAACA GCGCGCGAAA TATCTGCCGA AACTCATTTC CGGTGAGCAT GTCGGCGCGC TGGCCATGTC TGAGCCGGGC GCGGGGTCCG ATGTTGTTTC CATGAAGCTT GCCGCCGAAA AGCGCGGGGA CCGTTACGTC CTCAACGGCA ACAAGATGTG GATCACCAAT GGCCCGGATG CCGATGTGCT GGTGGTCTAT GCCAAGACAG ACCTGTCGGC TGGCCCGCGC GGGATCAGCG CCTTTATCAT TGAAAAAGGG TTCAAGGGTT TTTCGACCGC GCAAAAGCTC GACAAACTCG GTATGCGCGG TTCCAACACC TGCGAACTGG TGTTCGAGGA TTGCGAAGTG CCAGCCGAAA ACCTGCTCGG AACCGAGGGC AAGGGCGTCA ATGTGCTGAT GTCCGGCCTC GATTATGAGC GCGTGGTGCT GGCGGGTGGG CCGCTCGGCA TCATGGCGGC CTGCCTCGAT GTCGTGGTGC CCTATGTGCA TGAGCGCAAG CAGTTCGACC AGCCAATCGG TGAATTCCAG CTCATGCAGT GCAAGCTTGC CGACATGTAT GTGACCTTCA ACGCTTCACG CGCTTATGTC TATGCCGTGG CCGCAGCCTG CGACCGGGGT GAGACGACGC GCAAGGATGC GGCGGGCTGC ATTCTTTACT CTGCGGAAAA TGCCACGCAG ATGGCCTTGC AGGCGATCCA GTCTCTCGGC GGCAACGGCT ATATCAACGA TTATCCGACG GGCCGCCTTT TGCGCGATGC CAAGCTTTAT GAAATCGGCG CGGGCACGTC GGAAATCCGG CGTATGCTGA TCGGACGGGA ACTCTTTCAG GAGACCCGTT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT