BrsuA.00011.a

cell division protein FtsZ

CENTER ID:	BrsuA.00011.a
ORGANISM:	Brucella suis 1330
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrsuA.00011.a.A3.GE28187	Created from Decode construct for feature BrsuA.00011.a		13	566
BrsuA.00011.a.A4.GE28188	Created from Decode construct for feature BrsuA.00011.a		13	323

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_128807
PATRIC ID:	fig\|204722.5.peg.1451
RefSeq:	NP_698423
UniProt:	A0A0H3G7S8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTINLQKPDI TELKPRITVF GVGGGGGNAV NNMINAGLRG VDFVVANTDA QALTMSKSDR IIQLGAAVTE GLGAGSQPEV GRAAAEECID EIVDHLNGTH MCFVTAGMGG GTGTGAAPVV ARAARERGIL TVGVVTKPFH FEGARRMKTA DLGIEELQKN VDTLIVIPNQ NLFRIANDKT TFADAFAMAD QVLYSGVACI TDLMVKEGLI NLDFADVRSV MREMGKAMMG TGEASGEGRA MAAAEAAIAN PLLDETSMRG AKGLLISITG GRDMTLFEVD EAATRIREEV DPEANIILGA TFDEGLEGVI RVSVVATGID KQQGDAAPAP LEFRQPVKPT AAQAKPMAPH GALRPPVAEQ PRQADPVAQV IQAAEAEMPV SPAAPAASAE PEFRPQSRIF QAPAPEAFER APVARAPMQP AQAMHAPQPQ QYQQPQMHEQ PVREPRPAPR MPAVSDFPPV AQAEINARRA PQQPVQEEPR GPMGLLKRLT HGLSRREEEQ PAARLEPAQH REPGMRPAEP RRPMQQDSSI YAPRRGQLDD QGRPQPRTAS EEDQLEIPAF LRRQSN

NT Sequence

ATGACTATCA ATCTGCAAAA GCCGGATATC ACCGAGCTGA AGCCCCGCAT CACCGTATTC GGTGTCGGCG GCGGCGGCGG CAATGCGGTC AACAACATGA TCAATGCCGG CCTGCGCGGC GTGGATTTCG TTGTGGCCAA CACCGACGCG CAGGCTTTGA CCATGTCGAA GTCCGACCGC ATCATCCAGC TTGGCGCTGC CGTGACGGAA GGCCTCGGCG CCGGTTCGCA GCCGGAAGTT GGTCGTGCTG CCGCGGAAGA ATGCATCGAC GAAATCGTCG ATCACCTGAA CGGTACGCAT ATGTGCTTTG TGACCGCAGG CATGGGCGGC GGCACCGGCA CGGGCGCTGC GCCTGTCGTG GCACGCGCTG CCCGCGAACG CGGCATCCTC ACCGTCGGCG TTGTGACCAA GCCTTTCCAC TTTGAAGGCG CCCGCCGCAT GAAAACTGCC GATCTGGGCA TTGAAGAACT GCAGAAGAAT GTCGATACGC TCATCGTCAT TCCGAACCAG AACCTGTTCC GCATCGCCAA CGACAAGACC ACTTTTGCCG ACGCTTTTGC GATGGCTGAT CAGGTGCTCT ATTCGGGTGT TGCCTGCATC ACCGACCTGA TGGTCAAGGA AGGCCTGATT AACCTCGACT TCGCTGACGT CCGCTCGGTC ATGCGCGAAA TGGGCAAGGC CATGATGGGC ACGGGCGAAG CTTCCGGTGA AGGCCGTGCG ATGGCTGCTG CCGAGGCCGC AATCGCCAAT CCGCTGCTCG ACGAAACCTC CATGCGCGGT GCGAAGGGCC TTCTGATCTC GATCACCGGT GGCCGCGACA TGACCCTGTT CGAGGTCGAT GAAGCTGCGA CCCGTATTCG TGAAGAAGTT GACCCGGAAG CCAACATCAT CCTTGGCGCA ACCTTCGACG AAGGCCTCGA AGGCGTCATC CGTGTTTCCG TGGTTGCGAC CGGCATCGAC AAGCAGCAAG GAGATGCGGC GCCTGCGCCG CTGGAATTTC GCCAGCCAGT GAAGCCGACT GCCGCCCAGG CCAAGCCGAT GGCTCCGCAT GGCGCGCTGC GTCCGCCTGT TGCCGAACAG CCGCGTCAGG CCGATCCGGT CGCCCAGGTC ATCCAGGCCG CCGAAGCCGA AATGCCGGTT TCTCCGGCTG CGCCTGCCGC ATCGGCTGAG CCGGAATTCC GTCCGCAGAG CCGCATTTTC CAGGCTCCCG CGCCGGAAGC TTTTGAACGC GCCCCGGTTG CCCGCGCGCC GATGCAGCCG GCTCAGGCAA TGCATGCCCC CCAGCCGCAG CAGTATCAGC AGCCGCAGAT GCACGAACAG CCGGTTCGTG AGCCGCGCCC TGCACCGCGT ATGCCTGCCG TTTCCGACTT CCCGCCGGTG GCACAGGCAG AAATCAACGC CCGCCGTGCC CCGCAGCAGC CTGTACAGGA AGAGCCGCGC GGCCCGATGG GCCTTCTCAA GCGCCTGACC CATGGTCTGT CGCGCCGTGA GGAAGAACAG CCGGCTGCCC GTCTGGAGCC TGCACAGCAC CGCGAGCCGG GTATGCGCCC AGCCGAACCG CGCCGCCCGA TGCAGCAGGA TTCGTCGATC TATGCACCGC GCCGCGGTCA GCTTGACGAT CAGGGCCGTC CGCAGCCGCG GACAGCTTCT GAAGAAGATC AGCTCGAAAT CCCGGCTTTC CTGCGCCGCC AGTCGAAC

Details for BrsuA.00011.a.A3.GE28187

HARVESTED ON:	1/20/2010
SEQUENCED ON:	6/16/2010
EXPECTED MW:	61kDa
OBSERVED MW:	70kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	100
PERCENT COVERAGE:	53

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMLKPRITVF GVGGGNAVNN MINAGLRGVD FVVANTDAQA LTMSKSDRII QLGAAVTEGL GAGSQPEVGR AAAEECIDEI VDHLNGTHMC FVTAGMGGGT GTGAAPVVAR AARERGILTV GVVTKPFHFE GARRMKTADL GIEELQKNVD TLIVIPNQNL FRIANDKTTF ADAFAMADQV LYSGVACITD LMVKEGLINL DFADVRSVMR EMGKAMMGTG EASGEGRAMA AAEAAIANPL LDETSMRGAK GLLISITGGR DMTLFEVDEA ATRIREEVDP EANIILGA

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgctga agccccgcat caccgtattc ggtgtcggcg gcggcaatgc ggtcaacaac atgatcaatg ccggcctgcg cggcgtggat ttcgttgtgg ccaacaccga cgcgcaggct ttgaccatgt cgaagtccga ccgcatcatc cagcttggcg ctgccgtgac ggaaggcctc ggcgccggtt cgcagccgga agttggtcgt gctgccgcgg aagaatgcat cgacgaaatc gtcgatcacc tgaacggtac gcatatgtgc tttgtgaccg caggcatggg cggcggcacc ggcacgggcg ctgcgcctgt cgtggcacgc gctgcccgcg aacgcggcat cctcaccgtc ggcgttgtga ccaagccttt ccactttgaa ggcgcccgcc gcatgaaaac tgccgatctg ggcattgaag aactgcagaa gaatgtcgat acgctcatcg tcattccgaa ccagaacctg ttccgcatcg ccaacgacaa gaccactttt gccgacgctt ttgcgatggc tgatcaggtg ctctattcgg gtgttgcctg catcaccgac ctgatggtca aggaaggcct gattaacctc gacttcgctg acgtccgctc ggtcatgcgc gaaatgggca aggccatgat gggcacgggc gaggcttccg gtgaaggncg tgcgatggct gctgccgagg ccgcaatcgc caatccgctg ctcgacgaaa cctccatgcg cggtgcgaag ggccttctga tctcgatcac cggtggccgc gacatgaccc tgttcgaggt cgatgaagct gcgacccgta ttcgtgaaga agttgacccg gaagccaaca tcatccttgg cgcnac

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMLKPRITVF GVGGGGGNAV NNMINAGLRG VDFVVANTDA QALTMSKSDR IIQLGAAVTE GLGAGSQPEV GRAAAEECID EIVDHLNGTH MCFVTAGMGG GTGTGAAPVV ARAARERGIL TVGVVTKPFH FEGARRMKTA DLGIEELQKN VDTLIVIPNQ NLFRIANDKT TFADAFAMAD QVLYSGVACI TDLMVKEGLI NLDFADVRSV MREMGKAMMG TGEASGEGRA MAAAEAAIAN PLLDETSMRG AKGLLISITG GRDMTLFEVD EAATRIREEV DPEANIILGA TFDEGLEGVI RVSVVATGID KQQGDAAPAP LEFRQPVKPT AAQAKPMAPH GALRPPVAEQ PRQADPVAQV IQAAEAEMPV SPAAPAASAE PEFRPQSRIF QAPAPEAFER APVARAPMQP AQAMHAPQPQ QYQQPQMHEQ PVREPRPAPR MPAVSDFPPV AQAEINARRA PQQPVQEEPR GPMGLLKRLT HGLSRREEEQ PAARLEPAQH REPGMRPAEP RRPMQQDSSI YAPRRGQLDD QGRPQPRTAS EEDQLEIPAF LRRQSN

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gctgaagccc cgcatcaccg tattcggtgt cggcggcggc ggcggcaatg cggtcaacaa catgatcaat gccggcctgc gcggcgtgga tttcgttgtg gccaacaccg acgcgcaggc tttgaccatg tcgaagtccg accgcatcat ccagcttggc gctgccgtga cggaaggcct cggcgccggt tcgcagccgg aagttggtcg tgctgccgcg gaagaatgca tcgacgaaat cgtcgatcac ctgaacggta cgcatatgtg ctttgtgacc gcaggcatgg gcggcggcac cggcacgggc gctgcgcctg tcgtggcacg cgctgcccgc gaacgcggca tcctcaccgt cggcgttgtg accaagcctt tccactttga aggcgcccgc cgcatgaaaa ctgccgatct gggcattgaa gaactgcaga agaatgtcga tacgctcatc gtcattccga accagaacct gttccgcatc gccaacgaca agaccacttt tgccgacgct tttgcgatgg ctgatcaggt gctctattcg ggtgttgcct gcatcaccga cctgatggtc aaggaaggcc tgattaacct cgacttcgct gacgtccgct cggtcatgcg cgaaatgggc aaggccatga tgggcacggg cgaagcttcc ggtgaaggcc gtgcgatggc tgctgccgag gccgcaatcg ccaatccgct gctcgacgaa acctccatgc gcggtgcgaa gggccttctg atctcgatca ccggtggccg cgacatgacc ctgttcgagg tcgatgaagc tgcgacccgt attcgtgaag aagttgaccc ggaagccaac atcatccttg gcgcaacctt cgacgaaggc ctcgaaggcg tcatccgtgt ttccgtggtt gcgaccggca tcgacaagca gcaaggagat gcggcgcctg cgccgctgga atttcgccag ccagtgaagc cgactgccgc ccaggccaag ccgatggctc cgcatggcgc gctgcgtccg cctgttgccg aacagccgcg tcaggccgat ccggtcgccc aggtcatcca ggccgccgaa gccgaaatgc cggtttctcc ggctgcgcct gccgcatcgg ctgagccgga attccgtccg cagagccgca ttttccaggc tcccgcgccg gaagcttttg aacgcgcccc ggttgcccgc gcgccgatgc agccggctca ggcaatgcat gccccccagc cgcagcagta tcagcagccg cagatgcacg aacagccggt tcgtgagccg cgccctgcac cgcgtatgcc tgccgtttcc gacttcccgc cggtggcaca ggcagaaatc aacgcccgcc gtgccccgca gcagcctgta caggaagagc cgcgcggccc gatgggcctt ctcaagcgcc tgacccatgg tctgtcgcgc cgtgaggaag aacagccggc tgcccgtctg gagcctgcac agcaccgcga gccgggtatg cgcccagccg aaccgcgccg cccgatgcag caggattcgt cgatctatgc accgcgccgc ggtcagcttg acgatcaggg ccgtccgcag ccgcggacag cttctgaaga agatcagctc gaaatcccgg ctttcctgcg ccgccagtcg aacaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa

Details for BrsuA.00011.a.A4.GE28188

HARVESTED ON:	1/20/2010
SEQUENCED ON:	6/16/2010
EXPECTED MW:	34kDa
OBSERVED MW:	38kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	97
PERCENT COVERAGE:	99

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMLKPRITVF GVGGGGNAVN NMINAGLRGV DFVVANTDAQ ALTMSKSDRI IQLGAAVTEG LGAGSQPEVG RAAAEECIDE IVDHLNGTHM CFVTAGMGGG TGTGAAPVVA RAARERGILT VGVVTKPFHF EGARRMKTAD LGIEELQKNV DTLIVIPNQN LFRIANDKTT FADAFAMADQ VLYSGVACIT DLMVKEGLIN LDFADVRSVM REMGKAMMGT GEASGEGRAM AAAEAAIANP LLDETSMRGA KGLLISITGG RDMTLFEVDE AATRIREEVD PEANIXLGAT FDEXLXGVXR VXVVATGIDS AX

Validated NT Sequence

attttgttta nctttaagaa ggagatatac catggctcat caccatcacc atcatatggg taccctggaa gctcagaccc agggtcctgg ttcgatgctg aagccccgca tcaccgtatt cggtgtcggc ggcggcggca atgcggtcaa caacatgatc aatgccggcc tgcgcggcgt ggatttcgtt gtggccaaca ccgacgcgca ggctttgacc atgtcgaagt ccgaccgcat catccagctt ggcgctgccg tgacggaagg cctcggcgcc ggttcgcagc cggaagttgg tcgtgctgcc gcggaagaat gcatcgacga aatcgtcgat cacctgaacg gtacgcatat gtgctttgtg accgcaggca tgggcggcgg caccggcacg ggcgctgcgc ctgtcgtggc acgcgctgcc cgcgaacgcg gcatcctcac cgtcggcgtt gtgaccaagc ctttccactt tgaaggcgcc cgccgcatga aaactgccga tctgggcatt gaagaactgc agaagaatgt cgatacgctc atcgtcattc cgaaccagaa cctgttccgc atcgccaacg acaagaccac ttttgccgac gcttttgcga tggctgatca ggtgctctat tcgggtgttg cctgcatcac cgacctgatg gtcaaggaag gnctgattaa cctcgacttc gctgacgtcc gctcggtcat gcgcgaaatg ggcaaggcca tgatgggcac gggcgaggct tccggtgaag gccgtgcgat ggctgctgcc gaggccgcaa tcgccaatcc gctgctcgac gaaacctcca tgcgcggtgc gaagggcctt ctgatctcga tcaccggtgg ccgcgacatg accctgttcg aggtcgatga agctgcgacc cgtattcgtg aagaagttga cccggaagcc aacatcntcc ttggcgcaac cttcgacgaa nnnctcgang gngtcntccg tgtttncgtg gttgcnaccg gcatcgacag cgcanaataa ncagcacgaa cnngtnnngc agncnagctn cnnnnnngat ncnnctgcta ncaaannnng aaagnnngcn nnantngntn ctg

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gctgaagccc cgcatcaccg tattcggtgt cggcggcggc ggcggcaatg cggtcaacaa catgatcaat gccggcctgc gcggcgtgga tttcgttgtg gccaacaccg acgcgcaggc tttgaccatg tcgaagtccg accgcatcat ccagcttggc gctgccgtga cggaaggcct cggcgccggt tcgcagccgg aagttggtcg tgctgccgcg gaagaatgca tcgacgaaat cgtcgatcac ctgaacggta cgcatatgtg ctttgtgacc gcaggcatgg gcggcggcac cggcacgggc gctgcgcctg tcgtggcacg cgctgcccgc gaacgcggca tcctcaccgt cggcgttgtg accaagcctt tccactttga aggcgcccgc cgcatgaaaa ctgccgatct gggcattgaa gaactgcaga agaatgtcga tacgctcatc gtcattccga accagaacct gttccgcatc gccaacgaca agaccacttt tgccgacgct tttgcgatgg ctgatcaggt gctctattcg ggtgttgcct gcatcaccga cctgatggtc aaggaaggcc tgattaacct cgacttcgct gacgtccgct cggtcatgcg cgaaatgggc aaggccatga tgggcacggg cgaagcttcc ggtgaaggcc gtgcgatggc tgctgccgag gccgcaatcg ccaatccgct gctcgacgaa acctccatgc gcggtgcgaa gggccttctg atctcgatca ccggtggccg cgacatgacc ctgttcgagg tcgatgaagc tgcgacccgt attcgtgaag aagttgaccc ggaagccaac atcatccttg gcgcaacctt cgacgaaggc ctcgaaggcg tcatccgtgt ttccgtggtt gcgaccggca tcgacaagca gcaaaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa