| CENTER ID: | BrovA.18829.a |
| ORGANISM: | Brucella ovis ATCC 25840 |
| ASSOCIATED DISEASE: | Ovine epididymitis |
| CURRENT STATUS: | purified |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrovA.18829.a.B2.GE38169 | soluble domain | 17 | 161 | ||
| BrovA.18829.a.B3.GE39624 | shortened soluble domain | 23 | 157 |
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrovA.18829.a.B2.PS02201 | soluble domain | 17 | 161 | ||
| BrovA.18829.a.B3.PS37834 | shortened soluble domain | 23 | 157 | ||
| BrovA.18829.a.B3.PS37835 | shortened soluble domain | 23 | 157 |
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|444178.3.peg.2012 |
| RefSeq: | YP_001258672.1 |
| UniProt: | A0A0H3ANH0 |
| HARVESTED ON: | 6/11/2014 |
| SEQUENCED ON: | 6/12/2014 |
| EXPECTED MW: | 17kDa |
| OBSERVED MW: | 16kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with sequence variation |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
| HARVESTED ON: | 10/30/2015 |
| SEQUENCED ON: | 10/23/2015 |
| EXPECTED MW: | 16kDa |
| OBSERVED MW: | 16kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
| PURIFICATION DATe: | 11/6/2014 |
| CONCENTRATION: | 55.7mg/ml |
| OBSERVED MW: | 17kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 4 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
| PURIFICATION DATe: | 2/16/2016 |
| CONCENTRATION: | 60.3mg/ml |
| OBSERVED MW: | 16kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | SEC Buffer |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 2 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
| PURIFICATION DATe: | 2/16/2016 |
| CONCENTRATION: | 41.6mg/ml |
| OBSERVED MW: | 16kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | SEC Buffer |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 2 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |