BrovA.18198.b

Transcriptional regulator, DeoR family

CENTER ID: BrovA.18198.b
ORGANISM: Brucella ovis ATCC 25840
ASSOCIATED DISEASE: Ovine epididymitis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrovA.18198.b.B1.GE39085 Full length( BrovA.18198.b ) 1 254
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrovA.18198.b.B1.PS37815 Full length( BrovA.18198.b ) 1 254

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruOvi136990_0938
BV-BRC: fig|444178.3.peg.938
RefSeq: YP_001257799.1
UniProt: A0A0H3ATB8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKRDDRRQAI INLLIENQAV DLDDLAGRFA VSKMTIHRDL DSLEQSGVLR KVRGGATIDA GTQFESDFRF RERQDGEAKL KMARAALELV EPGMTVMVND GSMAALLGGM LREKRPLTVI TNNAVIIDEL KGENGINLIA LGGTYSAKFN AFFGILTEEA LARLSADIAF VSSPAVNGQL VYHMDENVVR TKRAMTNAAA RSCLLVNHQR FGRPALHVMA DLTDFDAIIT DASPGTAVLN GLKQAGIKLT IAAD
NT Sequence
GTGAAGCGCG ACGACAGACG ACAGGCGATC ATAAACCTTC TTATCGAAAA TCAGGCCGTC GATCTTGACG ATCTGGCGGG GCGGTTTGCC GTTTCCAAGA TGACGATCCA TCGTGATCTG GATTCACTGG AACAGTCCGG CGTATTGCGC AAGGTACGCG GCGGCGCGAC GATCGACGCG GGCACACAAT TCGAGAGTGA TTTCCGTTTT CGCGAGCGGC AGGACGGCGA AGCCAAGCTG AAGATGGCCC GCGCAGCACT TGAGCTTGTG GAACCCGGCA TGACCGTGAT GGTCAATGAT GGTTCCATGG CTGCCCTTCT GGGCGGCATG TTGCGGGAAA AGCGCCCGCT CACCGTCATC ACCAACAATG CCGTCATCAT TGATGAGCTG AAAGGTGAAA ACGGGATCAA CCTTATTGCG CTCGGCGGCA CCTATTCGGC AAAGTTCAAC GCCTTTTTCG GTATTTTGAC GGAAGAAGCA TTGGCGCGCC TCAGCGCCGA CATTGCCTTT GTCTCATCGC CTGCCGTCAA CGGGCAACTG GTCTATCACA TGGACGAAAA CGTGGTGCGC ACCAAGCGCG CCATGACGAA TGCCGCCGCA CGCTCGTGCC TTCTGGTCAA CCACCAGCGT TTCGGGCGCC CTGCCCTGCA TGTGATGGCC GACCTTACCG ATTTTGACGC GATCATCACC GATGCATCCC CCGGCACGGC GGTTTTGAAC GGACTGAAAC AGGCCGGGAT CAAACTGACA ATAGCAGCAG AC
Details for BrovA.18198.b.B1.GE39085
HARVESTED ON: 3/31/2015
SEQUENCED ON: 3/27/2015
EXPECTED MW: 29kDa
OBSERVED MW: 28kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMV KRDDRRQAII NLLIENQAVD LDDLAGRFAV SKMTIHRDLD SLEQSGVLRK VCGGATIDAG TQFESDFRFR ERQDGEAKLK MARAALELVE PGMTVMVNDG SMAALLGGML REKRPLTVIT NNAVIIDELK GENGINLIAL GGTYSAKFNA FFGILTEEAL ARLSADIAFV SSPAVNGQLV YHMDENVVRT KRAMTNAAAR SCLLVNHQRF GRPALHVMAD LTDFDAIITD ASPGTAVLNG LKQAGIKLTI AAD
Validated NT Sequence
atcttactca cttagtctgc tgctattgtc agtttgatcc cggcctgttt cagtccgttc aaaaccgccg tgccggggga tgcatcggtg atgatcgcgt caaaatcggt aaggtcggcc atcacatgca gggcagggcg cccgaaacgc tggtggttga ccagaaggca cgagcgtgcg gcggcattcg tcatggcgcg cttggtgcgc accacgtttt cgtccatgtg atagaccagt tgcccgttga cggcaggcga tgagacaaag gcaatgtcgg cgctgaggcg cgccaatgct tcttccgtca aaataccgaa aaaggcgttg aactttgccg aataggtgcc gccgagcgca ataaggttga tcccgttttc acctttcagc tcatcaatga tgacggcatt gttggtgatg acggtgagcg ggcgcttttc ccgcaacatg ccgcccagaa gggcagccat ggaaccatca ttgaccatca cggtcatgcc gggttccaca agctcaagtg ctgcgcgggc catcttcagc ttggcttcgc cgtcctgccg ctcgcgaaaa cggaaatcac tctcgaattg tgtgcccgcg tcgatcgtcg cgccgccgca taccttgcgc agtacgccgg actgttccag tgaatccaga tcacgatgga tcgtcatctt ggaaacggca aaccgccccg ccagatcgtc aagatcgacg gcctgatttt cgataagaag gtttatgatc gcctgtcgtc tgtcgtcgcg cttcaccata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHMK RDDRRQAIIN LLIENQAVDL DDLAGRFAVS KMTIHRDLDS LEQSGVLRKV RGGATIDAGT QFESDFRFRE RQDGEAKLKM ARAALELVEP GMTVMVNDGS MAALLGGMLR EKRPLTVITN NAVIIDELKG ENGINLIALG GTYSAKFNAF FGILTEEALA RLSADIAFVS SPAVNGQLVY HMDENVVRTK RAMTNAAARS CLLVNHQRFG RPALHVMADL TDFDAIITDA SPGTAVLNGL KQAGIKLTIA AD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgaagc gcgacgacag acgacaggcg atcataaacc ttcttatcga aaatcaggcc gtcgatcttg acgatctggc ggggcggttt gccgtttcca agatgacgat ccatcgtgat ctggattcac tggaacagtc cggcgtattg cgcaaggtac gcggcggcgc gacgatcgac gcgggcacac aattcgagag tgatttccgt tttcgcgagc ggcaggacgg cgaagccaag ctgaagatgg cccgcgcagc acttgagctt gtggaacccg gcatgaccgt gatggtcaat gatggttcca tggctgccct tctgggcggc atgttgcggg aaaagcgccc gctcaccgtc atcaccaaca atgccgtcat cattgatgag ctgaaaggtg aaaacgggat caaccttatt gcgctcggcg gcacctattc ggcaaagttc aacgcctttt tcggtatttt gacggaagaa gcattggcgc gcctcagcgc cgacattgcc tttgtctcat cgcctgccgt caacgggcaa ctggtctatc acatggacga aaacgtggtg cgcaccaagc gcgccatgac gaatgccgcc gcacgctcgt gccttctggt caaccaccag cgtttcgggc gccctgccct gcatgtgatg gccgacctta ccgattttga cgcgatcatc accgatgcat cccccggcac ggcggttttg aacggactga aacaggccgg gatcaaactg acaatagcag cagactgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for BrovA.18198.b.B1.PS37815
PURIFICATION DATe: 1/28/2016
CONCENTRATION: 13.65mg/ml
OBSERVED MW: 28kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: SEC buffer
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 110µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMV KRDDRRQAII NLLIENQAVD LDDLAGRFAV SKMTIHRDLD SLEQSGVLRK VCGGATIDAG TQFESDFRFR ERQDGEAKLK MARAALELVE PGMTVMVNDG SMAALLGGML REKRPLTVIT NNAVIIDELK GENGINLIAL GGTYSAKFNA FFGILTEEAL ARLSADIAFV SSPAVNGQLV YHMDENVVRT KRAMTNAAAR SCLLVNHQRF GRPALHVMAD LTDFDAIITD ASPGTAVLNG LKQAGIKLTI AAD
Validated NT Sequence
atcttactca cttagtctgc tgctattgtc agtttgatcc cggcctgttt cagtccgttc aaaaccgccg tgccggggga tgcatcggtg atgatcgcgt caaaatcggt aaggtcggcc atcacatgca gggcagggcg cccgaaacgc tggtggttga ccagaaggca cgagcgtgcg gcggcattcg tcatggcgcg cttggtgcgc accacgtttt cgtccatgtg atagaccagt tgcccgttga cggcaggcga tgagacaaag gcaatgtcgg cgctgaggcg cgccaatgct tcttccgtca aaataccgaa aaaggcgttg aactttgccg aataggtgcc gccgagcgca ataaggttga tcccgttttc acctttcagc tcatcaatga tgacggcatt gttggtgatg acggtgagcg ggcgcttttc ccgcaacatg ccgcccagaa gggcagccat ggaaccatca ttgaccatca cggtcatgcc gggttccaca agctcaagtg ctgcgcgggc catcttcagc ttggcttcgc cgtcctgccg ctcgcgaaaa cggaaatcac tctcgaattg tgtgcccgcg tcgatcgtcg cgccgccgca taccttgcgc agtacgccgg actgttccag tgaatccaga tcacgatgga tcgtcatctt ggaaacggca aaccgccccg ccagatcgtc aagatcgacg gcctgatttt cgataagaag gtttatgatc gcctgtcgtc tgtcgtcgcg cttcaccata tggtggtggt ggtggtgagc cat
Expressed Protein Sequence
MAHHHHHHMK RDDRRQAIIN LLIENQAVDL DDLAGRFAVS KMTIHRDLDS LEQSGVLRKV RGGATIDAGT QFESDFRFRE RQDGEAKLKM ARAALELVEP GMTVMVNDGS MAALLGGMLR EKRPLTVITN NAVIIDELKG ENGINLIALG GTYSAKFNAF FGILTEEALA RLSADIAFVS SPAVNGQLVY HMDENVVRTK RAMTNAAARS CLLVNHQRFG RPALHVMADL TDFDAIITDA SPGTAVLNGL KQAGIKLTIA AD
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGTGAAGC GCGACGACAG ACGACAGGCG ATCATAAACC TTCTTATCGA AAATCAGGCC GTCGATCTTG ACGATCTGGC GGGGCGGTTT GCCGTTTCCA AGATGACGAT CCATCGTGAT CTGGATTCAC TGGAACAGTC CGGCGTATTG CGCAAGGTAC GCGGCGGCGC GACGATCGAC GCGGGCACAC AATTCGAGAG TGATTTCCGT TTTCGCGAGC GGCAGGACGG CGAAGCCAAG CTGAAGATGG CCCGCGCAGC ACTTGAGCTT GTGGAACCCG GCATGACCGT GATGGTCAAT GATGGTTCCA TGGCTGCCCT TCTGGGCGGC ATGTTGCGGG AAAAGCGCCC GCTCACCGTC ATCACCAACA ATGCCGTCAT CATTGATGAG CTGAAAGGTG AAAACGGGAT CAACCTTATT GCGCTCGGCG GCACCTATTC GGCAAAGTTC AACGCCTTTT TCGGTATTTT GACGGAAGAA GCATTGGCGC GCCTCAGCGC CGACATTGCC TTTGTCTCAT CGCCTGCCGT CAACGGGCAA CTGGTCTATC ACATGGACGA AAACGTGGTG CGCACCAAGC GCGCCATGAC GAATGCCGCC GCACGCTCGT GCCTTCTGGT CAACCACCAG CGTTTCGGGC GCCCTGCCCT GCATGTGATG GCCGACCTTA CCGATTTTGA CGCGATCATC ACCGATGCAT CCCCCGGCAC GGCGGTTTTG AACGGACTGA AACAGGCCGG GATCAAACTG ACAATAGCAG CAGACTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT