BrovA.18190.a

UDP-glucose 4-epimerase (EC 5.1.3.2)

CENTER ID: BrovA.18190.a
ORGANISM: Brucella ovis ATCC 25840
ASSOCIATED DISEASE: Ovine epididymitis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrovA.18190.a.B1.GE38428 Full length( BrovA.18190.a ) 1 328
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruOvi136990_0444
BV-BRC: fig|444178.3.peg.444
RefSeq: YP_001257418.1
UniProt: A0A0H3AU76

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNMKVLVTGS QGYIGSVMVP MLIDAGHEVS GYDIGLYEHC LFKEGGTVMN VPTIRKDVRD VSPRDLEGFD AVIHLAALSN DPLGNLNEDL TYAINHHASV AMAKAAKLAG VGRFLFASSC SNYGISDAEL IDETGELKPV TAYGRSKVRA EQEIRKLADA SFCPVYLRPA TAYGVSPFLR FDIVLNNLVA WAVTKGLIYL KSDGTPWRPI VHIHDISRAF MAAMEAPREK VWNEAFNVGS TAHNYRIRDI AGVVADNVPD CRIEYAQDAV AGTRELLAAY SRSTLTVEDF EGPRFQRIAH IRKLIAQGVL DADLCRMDAH SHPLRAMA
NT Sequence
ATGAACATGA AAGTACTGGT CACGGGAAGT CAGGGTTATA TCGGCTCGGT CATGGTGCCG ATGCTCATCG ATGCCGGGCA TGAGGTGTCG GGCTATGATA TCGGCCTTTA CGAGCATTGC CTGTTCAAGG AAGGCGGGAC GGTCATGAAT GTGCCCACCA TCCGCAAGGA TGTGCGCGAT GTCTCGCCGC GCGATCTGGA AGGTTTCGAT GCGGTCATCC ATCTTGCCGC GCTTTCCAAC GATCCGCTGG GCAACCTCAA CGAAGACCTG ACCTATGCCA TCAACCACCA CGCCAGCGTC GCCATGGCAA AGGCCGCAAA ACTTGCTGGG GTAGGGCGCT TTCTCTTTGC CTCATCATGC AGCAATTACG GTATCAGCGA TGCCGAACTG ATCGACGAAA CCGGCGAATT GAAGCCTGTC ACAGCCTATG GCCGCTCCAA GGTGCGGGCG GAACAGGAAA TCCGTAAGCT TGCGGATGCG AGCTTCTGCC CGGTCTATCT GCGGCCCGCA ACCGCTTATG GTGTTTCACC CTTCCTGCGC TTCGATATCG TGCTGAACAA TCTCGTTGCC TGGGCGGTGA CGAAGGGGCT GATCTATCTG AAATCCGACG GCACACCCTG GCGGCCCATC GTGCATATCC ACGATATTTC ACGCGCTTTC ATGGCGGCGA TGGAAGCGCC GCGCGAAAAG GTCTGGAATG AAGCGTTCAA TGTCGGCTCT ACCGCGCATA ACTACCGTAT TCGCGATATT GCCGGGGTCG TGGCCGATAA TGTGCCGGAT TGCCGGATCG AATATGCGCA GGATGCGGTG GCGGGCACGC GTGAATTGCT TGCTGCCTAC AGCCGATCAA CCCTGACAGT CGAGGACTTT GAAGGGCCGC GCTTTCAGCG CATTGCCCAT ATTCGCAAGC TGATAGCGCA AGGCGTTCTG GATGCCGACC TGTGCCGCAT GGACGCCCAT TCGCACCCCC TGCGGGCGAT GGCA
Details for BrovA.18190.a.B1.GE38428
HARVESTED ON: 8/29/2014
SEQUENCED ON: 12/10/2014
EXPECTED MW: 37kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 105
Validated AA Sequence
MAHHHHHMNM KVLVTGSQGY IGSVMVPMLI DAGHEVSGYD IGLYEHCLFK EGGTVMNVPT IRKDVRDVSP RDLEGFDAVI HLAALSNDPL GNLNEDLTYA INHHASVAMA KAAKLAGVGR FLFASSCSNY GISDAELIDE TGELKPVTAY GRSKVRAEQE IRKLADASFC PVYLRPATAY GVSPFLRFDI VLNNLVAWAV TKGLIYLKSD GTPWRPIVHI HDISRAFMAA MEAPREKVWN EAFNVGSTAH NYRIRDIAGV VADNVPDCRI EYAQDAGPDT RSYRVSFEKL AHVLPEAKPQ WDAVAGTREL LAAYSRSTLT VEEFEGPRFQ RIAHIRKLIA QGVLDADLRR MDAH
Validated NT Sequence
aatgggcgtc catgcggcgc aggtcggcat ccagaacgcc ttgcgctatc agcttgcgaa tatgggcaat gcgctgaaag cgcggccctt caaattcctc gactgtcagg gttgatcggc tgtaggcagc aagcaattca cgcgtgcccg ccaccgcatc ccattgcggt tttgcttccg gcagcacatg cgccagcttt tcaaaactga cgcgatagga ccgcgtatcc ggccccgcat cctgcgcata ttcgatccgg caatccggca cattatcggc cacgaccccg gcaatatcgc gaatacggta gttatgcgcg gtagagccga cattgaacgc ttcattccag accttttcgc gcggcgcttc catcgccgcc atgaaagcgc gtgaaatatc gtggatatgc acgatgggcc gccagggtgt gccgtcggat ttcagataga tcagcccctt cgtcaccgcc caggcaacga gattgttcag cacgatatcg aagcgcagga agggtgaaac accataggcg gttgcgggcc gcagatagac cgggcagaag ctcgcatccg caagcttacg gatttcctgt tccgcccgca ccttggagcg gccataggct gtgacaggct tcaattcgcc ggtttcgtcg atcagttcgg catcgctgat accgtaattg ctgcatgatg aggcaaagag aaagcgccct accccagcaa gttttgcggc ctttgccatg gcgacgctgg cgtggtggtt gatggcatag gtcaggtctt cgttgaggtt gcccagcgga tcgttggaaa gcgcggcaag atggatgacc gcatcgaaac cttccagatc gcgcggcgag acatcgcgca catccttgcg gatggtgggc acattcatga ccgtcccgcc ttccttgaac aggcaatgct cgtaaaggcc gatatcatag cccgacacct catgcccggc atcgatgagc atcggcacca tgaccgagcc gatataaccc tgacttcccg tgaccagtac tttcatgttc atatggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHMN MKVLVTGSQG YIGSVMVPML IDAGHEVSGY DIGLYEHCLF KEGGTVMNVP TIRKDVRDVS PRDLEGFDAV IHLAALSNDP LGNLNEDLTY AINHHASVAM AKAAKLAGVG RFLFASSCSN YGISDAELID ETGELKPVTA YGRSKVRAEQ EIRKLADASF CPVYLRPATA YGVSPFLRFD IVLNNLVAWA VTKGLIYLKS DGTPWRPIVH IHDISRAFMA AMEAPREKVW NEAFNVGSTA HNYRIRDIAG VVADNVPDCR IEYAQDAVAG TRELLAAYSR STLTVEDFEG PRFQRIAHIR KLIAQGVLDA DLCRMDAHSH PLRAMA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaca tgaaagtact ggtcacggga agtcagggtt atatcggctc ggtcatggtg ccgatgctca tcgatgccgg gcatgaggtg tcgggctatg atatcggcct ttacgagcat tgcctgttca aggaaggcgg gacggtcatg aatgtgccca ccatccgcaa ggatgtgcgc gatgtctcgc cgcgcgatct ggaaggtttc gatgcggtca tccatcttgc cgcgctttcc aacgatccgc tgggcaacct caacgaagac ctgacctatg ccatcaacca ccacgccagc gtcgccatgg caaaggccgc aaaacttgct ggggtagggc gctttctctt tgcctcatca tgcagcaatt acggtatcag cgatgccgaa ctgatcgacg aaaccggcga attgaagcct gtcacagcct atggccgctc caaggtgcgg gcggaacagg aaatccgtaa gcttgcggat gcgagcttct gcccggtcta tctgcggccc gcaaccgctt atggtgtttc acccttcctg cgcttcgata tcgtgctgaa caatctcgtt gcctgggcgg tgacgaaggg gctgatctat ctgaaatccg acggcacacc ctggcggccc atcgtgcata tccacgatat ttcacgcgct ttcatggcgg cgatggaagc gccgcgcgaa aaggtctgga atgaagcgtt caatgtcggc tctaccgcgc ataactaccg tattcgcgat attgccgggg tcgtggccga taatgtgccg gattgccgga tcgaatatgc gcaggatgcg gtggcgggca cgcgtgaatt gcttgctgcc tacagccgat caaccctgac agtcgaggac tttgaagggc cgcgctttca gcgcattgcc catattcgca agctgatagc gcaaggcgtt ctggatgccg acctgtgccg catggacgcc cattcgcacc ccctgcgggc gatggcatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at