SSGCID: Target Page

BrovA.18144.b

Cobalamin synthesis protein/P47K family protein

CENTER ID:	BrovA.18144.b
ORGANISM:	Brucella ovis ATCC 25840
ASSOCIATED DISEASE:	Ovine epididymitis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrovA.18144.b.B1.GE38421	Full length( BrovA.18144.b )		1	386

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrovA.18144.b.B1.PW37604	Full length( BrovA.18144.b )		1	386

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	PATRIC_Locus_Tag:VBIBruOvi136990_3391
BV-BRC:	fig\|444178.3.peg.3391
RefSeq:	YP_001259846.1
UniProt:	A0A0H3ARJ1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPDPIPVSVL TGFLGSGKTT LLNRLLKDPA LSDTAVIINE FGEVSIDHLL VEQASEGVIE LADGCLCCTV RGELVDTLAD LIDRLQTGRI KALKRVIIET TGLGDPAPVL HSIMGHPLLT QVFRLDGVLA TVDAVNGMAT LDNHEEAVKQ AAMADRIILT KTDLPEAQAG LPALKARLRT LNPGADILEA GEERTGYAAL FECGLYNPQT KSADVRRWLK AEAYEDEHHG HGHVCGPDCG HDHHHHDHHH DQGYAHHHHH DDAIRSFSLR HDAPIPVSTF EMFLDLLRST HGEKLLRMKG IVQIAEDPDR PVVIHGVQKI FHPPARLPQW PQGKRETLLV LIVKDLPEAY VRELFDAFLG RPGLDRPDRA AMTENPLAIP GFSPRR

NT Sequence

ATGCCTGATC CCATTCCCGT TTCCGTGCTG ACCGGCTTTC TTGGCTCCGG CAAGACGACG CTGCTCAACC GGCTTTTGAA AGATCCGGCG CTCAGCGATA CAGCAGTCAT TATCAACGAG TTCGGCGAGG TGAGCATTGA CCATCTGCTG GTCGAGCAGG CGAGCGAGGG CGTGATCGAG CTGGCTGACG GGTGTCTGTG CTGCACAGTG CGCGGTGAAC TGGTCGATAC GCTGGCCGAT CTCATCGACC GGTTGCAGAC CGGCCGCATC AAGGCGCTGA AGCGCGTCAT CATCGAAACC ACGGGGCTTG GCGATCCGGC CCCGGTGCTG CATTCGATCA TGGGTCATCC TCTTCTCACG CAGGTTTTCC GGCTTGATGG CGTGCTGGCT ACCGTGGATG CGGTGAACGG CATGGCAACG CTCGACAATC ATGAGGAAGC CGTCAAACAG GCAGCCATGG CCGACCGCAT CATCCTCACC AAGACCGATC TGCCGGAAGC ACAGGCTGGC TTGCCGGCAT TGAAGGCACG GTTGCGCACG CTTAACCCCG GAGCCGATAT TCTGGAAGCG GGGGAAGAGC GCACAGGCTA TGCCGCGCTT TTTGAATGCG GGCTTTATAA CCCGCAGACG AAATCGGCGG ATGTGCGGCG CTGGCTTAAG GCCGAAGCCT ATGAGGACGA GCATCATGGC CATGGCCATG TGTGCGGGCC GGATTGCGGT CACGACCATC ATCACCACGA TCACCATCAC GATCAGGGCT ATGCGCATCA TCATCATCAT GACGATGCGA TCCGTTCGTT TTCACTGCGC CATGATGCGC CGATCCCGGT TTCGACCTTC GAGATGTTTC TCGATCTTCT GCGTTCGACC CATGGTGAAA AGCTCTTGCG CATGAAGGGT ATCGTGCAGA TTGCCGAAGA CCCGGACCGC CCGGTCGTCA TCCATGGTGT GCAGAAGATT TTCCATCCGC CCGCGCGCCT GCCGCAATGG CCGCAAGGCA AGCGCGAAAC GCTTCTGGTC CTGATTGTGA AGGACTTGCC GGAGGCCTAT GTGCGGGAAC TTTTCGATGC CTTTCTGGGC CGGCCCGGCC TCGACAGGCC CGACCGCGCA GCCATGACTG AAAATCCGCT GGCCATTCCG GGGTTCTCAC CCCGCCGG

Details for BrovA.18144.b.B1.GE38421

HARVESTED ON:	8/29/2014
SEQUENCED ON:	12/10/2014
EXPECTED MW:	44kDa
OBSERVED MW:	42kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMP DPIPVSVLTG FLGSGKTTLL NRLLKDPALS DTAVIINEFG EVSIDHLLVE QASEGVIELA DGCLCCTVRG ELVDTLADLI DRLQTGRIKA LKRVIIETTG LGDPAPVLHS IMGHPLLTQV FRLDGVLATV DAVNGMATLD NHEEAVKQAA MADRIILTKT DLPEAQAGLP ALKARLRTLN PGADILEAGE ERTGYAALFE CGLYNPQTKS ADVRRWLKAE AYEDEHHGHG HVCGPDCGHD HHHHDHHHDQ GYAHHHHHDD AIRSFSLRHD APIPVSTFEM FLDLLRSTHG EKLLRMKGIV QIAEDPDRPV VIHGVQKIFH PPARLPQWPQ GKRETLLVLI VKDLPEAYVR ELFDAFLGRP GLDRPDRAAM TENPLAIPGF SPRR

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcctg atcccattcc cgtttccgtg ctgaccggct ttcttggctc cggcaagacg acgctgctca accggctttt gaaagatccg gcgctcagcg atacagcagt cattatcaac gagttcggcg aggtgagcat tgaccatctg ctggtcgagc aggcgagcga gggcgtgatc gagctggctg acgggtgtct gtgctgcaca gtgcgcggtg aactggtcga tacgctggcc gatctcatcg accggttgca gaccggccgc atcaaggcgc tgaagcgcgt catcatcgaa accacggggc ttggcgatcc ggccccggtg ctgcattcga tcatgggtca tcctcttctc acgcaggttt tccggcttga tggcgtgctg gctaccgtgg atgcggtgaa cggcatggca acgctcgaca atcatgagga agccgtcaaa caggcagcca tggccgaccg catcatcctc accaagaccg atctgccgga agcacaggct ggcttgccgg cattgaaggc acggttgcgc acgcttaacc ccggagccga tattctggaa gcgggggaag agcgcacagg ctatgccgcg ctttttgaat gcgggcttta taacccgcag acgaaatcgg cggatgtgcg gcgctggctt aaggccgaag cctatgagga cgagcatcat ggccatggcc atgtgtgcgg gccggattgc ggtcacgacc atcatcacca cgatcaccat cacgatcagg gctatgcgca tcatcatcat catgacgatg cgatccgttc gttttcactg cgccatgatg cgccgatccc ggtttcgacc ttcgagatgt ttctcgatct tctgcgttcg acccatggtg aaaagctctt gcgcatgaag ggtatcgtgc agattgccga agacccggac cgcccggtcg tcatccatgg tgtgcagaag attttccatc cgcccgcgcg cctgccgcaa tggccgcaag gcaagcgcga aacgcttctg gtcctgattg tgaaggactt gccggaggcc tatgtgcggg aacttttcga tgcctttctg ggccggcccg gcctcgacag gcccgaccgc gcagccatga ctgaaaatcc gctggccatt ccggggttct caccccgccg gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat

Details for BrovA.18144.b.B1.PW37604

PURIFICATION DATe:	11/24/2014
CONCENTRATION:	10mg/ml
OBSERVED MW:	46kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	7
VIAL VOLUME:	200µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCCTG ATCCCATTCC CGTTTCCGTG CTGACCGGCT TTCTTGGCTC CGGCAAGACG ACGCTGCTCA ACCGGCTTTT GAAAGATCCG GCGCTCAGCG ATACAGCAGT CATTATCAAC GAGTTCGGCG AGGTGAGCAT TGACCATCTG CTGGTCGAGC AGGCGAGCGA GGGCGTGATC GAGCTGGCTG ACGGGTGTCT GTGCTGCACA GTGCGCGGTG AACTGGTCGA TACGCTGGCC GATCTCATCG ACCGGTTGCA GACCGGCCGC ATCAAGGCGC TGAAGCGCGT CATCATCGAA ACCACGGGGC TTGGCGATCC GGCCCCGGTG CTGCATTCGA TCATGGGTCA TCCTCTTCTC ACGCAGGTTT TCCGGCTTGA TGGCGTGCTG GCTACCGTGG ATGCGGTGAA CGGCATGGCA ACGCTCGACA ATCATGAGGA AGCCGTCAAA CAGGCAGCCA TGGCCGACCG CATCATCCTC ACCAAGACCG ATCTGCCGGA AGCACAGGCT GGCTTGCCGG CATTGAAGGC ACGGTTGCGC ACGCTTAACC CCGGAGCCGA TATTCTGGAA GCGGGGGAAG AGCGCACAGG CTATGCCGCG CTTTTTGAAT GCGGGCTTTA TAACCCGCAG ACGAAATCGG CGGATGTGCG GCGCTGGCTT AAGGCCGAAG CCTATGAGGA CGAGCATCAT GGCCATGGCC ATGTGTGCGG GCCGGATTGC GGTCACGACC ATCATCACCA CGATCACCAT CACGATCAGG GCTATGCGCA TCATCATCAT CATGACGATG CGATCCGTTC GTTTTCACTG CGCCATGATG CGCCGATCCC GGTTTCGACC TTCGAGATGT TTCTCGATCT TCTGCGTTCG ACCCATGGTG AAAAGCTCTT GCGCATGAAG GGTATCGTGC AGATTGCCGA AGACCCGGAC CGCCCGGTCG TCATCCATGG TGTGCAGAAG ATTTTCCATC CGCCCGCGCG CCTGCCGCAA TGGCCGCAAG GCAAGCGCGA AACGCTTCTG GTCCTGATTG TGAAGGACTT GCCGGAGGCC TATGTGCGGG AACTTTTCGA TGCCTTTCTG GGCCGGCCCG GCCTCGACAG GCCCGACCGC GCAGCCATGA CTGAAAATCC GCTGGCCATT CCGGGGTTCT CACCCCGCCG GTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT