BrovA.18113.b

COG0028: Thiamine pyrophosphate-requiring enzymes

CENTER ID: BrovA.18113.b
ORGANISM: Brucella ovis ATCC 25840
ASSOCIATED DISEASE: Ovine epididymitis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrovA.18113.b.B1.GE38414 Full length( BrovA.18113.b ) 1 449
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruOvi136990_1020

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAEAYGKLTG KPGICFVTRG PGATNASIGV HTAFQDSTPM LLFIGQVASD QVEREAFQEI DYRRMFGQMA KWVVQIDDAA RIPELVSQAF HRAVNGRPGP VVVALPEDML TSYAAVSDAP AYKQVAMHPG GEQLQAMRAL IEKAERPLMI VGGGGWTQDA VRDLRQFAEN MSLPVAASFR CQDMFDNTHP LYAGEMGTSI SARLVQRVKD SALLVVVGAR LGEMTTQGYE LVTIPVPQQK LVHIHPGAEE LGRVYHADVP INAGMPAFCA AAAKLAPVAD PRWKAWADSA NADYRDNIKS PVIPGEVQMG EVMEWLRAHL PADAIITNGA GNYSAWPHRF YQYRTYRSQL APTNGSMGYG VPAAVAAKLT APERTVVAFA GDGCFLMNGQ ELATAAQYGA NAIFIVVNNG MYGTSVCIRS APIRRAFRVR PWPIPTLRRL RGPMGCMVK
NT Sequence
ATGGCAGAGG CCTATGGCAA ACTGACGGGT AAGCCCGGCA TTTGCTTCGT CACGCGCGGC CCCGGCGCCA CCAATGCATC GATTGGCGTG CACACGGCTT TTCAGGATTC GACGCCCATG CTGCTCTTCA TCGGTCAGGT CGCAAGCGAT CAGGTCGAGC GTGAGGCATT TCAGGAAATC GATTATCGCC GCATGTTCGG CCAGATGGCC AAATGGGTCG TGCAGATTGA CGATGCCGCG CGCATTCCCG AACTGGTGAG CCAGGCATTC CATCGCGCGG TGAACGGGCG TCCCGGTCCG GTGGTGGTCG CCTTGCCGGA AGACATGCTG ACGTCCTATG CGGCGGTCAG CGATGCGCCT GCCTATAAGC AGGTGGCCAT GCATCCGGGT GGCGAACAGT TGCAGGCAAT GCGGGCGCTG ATCGAAAAGG CAGAGCGTCC GCTGATGATC GTCGGCGGCG GTGGCTGGAC ACAGGATGCC GTGCGCGACC TGCGCCAGTT TGCCGAAAAT ATGTCTCTGC CGGTTGCCGC ATCCTTCCGC TGTCAGGATA TGTTCGACAA TACGCATCCC CTTTATGCGG GCGAAATGGG GACCTCGATC AGTGCCAGGC TTGTCCAGCG GGTGAAGGAT TCCGCCCTGC TTGTCGTGGT TGGCGCGCGC CTTGGTGAAA TGACGACACA GGGCTATGAA CTCGTCACCA TTCCGGTGCC GCAGCAGAAG CTTGTCCATA TCCATCCGGG CGCGGAGGAA CTGGGCCGGG TCTATCATGC CGATGTTCCG ATCAATGCCG GGATGCCTGC CTTCTGTGCT GCGGCTGCAA AGCTTGCGCC GGTTGCCGAT CCGCGCTGGA AGGCATGGGC CGACAGCGCC AATGCCGACT ATCGCGACAA TATCAAGTCG CCGGTCATTC CGGGCGAAGT GCAGATGGGC GAGGTGATGG AGTGGCTGCG CGCGCATCTG CCCGCCGATG CCATCATCAC CAATGGCGCG GGCAATTATT CCGCATGGCC GCATCGCTTT TACCAGTATC GCACCTATCG CAGCCAGTTG GCCCCGACCA ATGGCTCCAT GGGCTATGGT GTGCCGGCTG CTGTTGCGGC CAAGCTCACC GCGCCGGAGC GGACCGTGGT GGCTTTCGCG GGAGATGGCT GTTTCCTCAT GAACGGGCAG GAGCTTGCAA CTGCCGCGCA ATATGGCGCG AATGCCATTT TCATCGTCGT GAACAACGGC ATGTATGGCA CGTCCGTATG CATCAGGAGC GCACCTATCC GGCGCGCATT TCGGGTACGG CCCTGGCCAA TCCCGACTTT GCGGCGCTTG CGCGGACCTA TGGGCTGCAT GGTGAAG
Details for BrovA.18113.b.B1.GE38414
HARVESTED ON: 8/29/2014
SEQUENCED ON: 12/10/2014
EXPECTED MW: 50kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMA EAYGKLTGKP GICFVTRGPG ATNASIGVHT AFQDSTPMLL FIGQVASDQV EREAFQEIDY RRMFGQMAKW VVQIDDAARI PELVSQAFHR AVNGRPGPVV VALPEDMLTS YAAVSDAPAY KQVAMHPGGE QLQAMRALIE KAERPLMIVG GGGWTQDAVR DLRQFAENMS LPVAASFRCQ DMFDNTHPLY AGEMGTSISA RLVQRVKDSA LLVVVGARLG EMTTQGYELV TIPVPQQKLV HIHPGAEELG RVYHADVPIN AGMPAFCAAA AKLAPVADPR WKAWADSANA DYRDNIKSPV IPGEVQMGEV MEWLRAHLPA DAIITNGAGN YSAWPHRFYQ YRTYRSQLAP TNGSMGYGVP AAVAAKLTAP ERTVVAFAGD GCFLMNGQEL ATAAQYGANA IFIVVNNGMY GTSVCIRSAP IRRAFRVRPW PIPTLRRLRG PMGCMVK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcag aggcctatgg caaactgacg ggtaagcccg gcatttgctt cgtcacgcgc ggccccggcg ccaccaatgc atcgattggc gtgcacacgg cttttcagga ttcgacgccc atgctgctct tcatcggtca ggtcgcaagc gatcaggtcg agcgtgaggc atttcaggaa atcgattatc gccgcatgtt cggccagatg gccaaatggg tcgtgcagat tgacgatgcc gcgcgcattc ccgaactggt gagccaggca ttccatcgcg cggtgaacgg gcgtcccggt ccggtggtgg tcgccttgcc ggaagacatg ctgacgtcct atgcggcggt cagcgatgcg cctgcctata agcaggtggc catgcatccg ggtggcgaac agttgcaggc aatgcgggcg ctgatcgaaa aggcagagcg tccgctgatg atcgtcggcg gcggtggctg gacacaggat gccgtgcgcg acctgcgcca gtttgccgaa aatatgtctc tgccggttgc cgcatccttc cgctgtcagg atatgttcga caatacgcat cccctttatg cgggcgaaat ggggacctcg atcagtgcca ggcttgtcca gcgggtgaag gattccgccc tgcttgtcgt ggttggcgcg cgccttggtg aaatgacgac acagggctat gaactcgtca ccattccggt gccgcagcag aagcttgtcc atatccatcc gggcgcggag gaactgggcc gggtctatca tgccgatgtt ccgatcaatg ccgggatgcc tgccttctgt gctgcggctg caaagcttgc gccggttgcc gatccgcgct ggaaggcatg ggccgacagc gccaatgccg actatcgcga caatatcaag tcgccggtca ttccgggcga agtgcagatg ggcgaggtga tggagtggct gcgcgcgcat ctgcccgccg atgccatcat caccaatggc gcgggcaatt attccgcatg gccgcatcgc ttttaccagt atcgcaccta tcgcagccag ttggccccga ccaatggctc catgggctat ggtgtgccgg ctgctgttgc ggccaagctc accgcgccgg agcggaccgt ggtggctttc gcgggagatg gctgtttcct catgaacggg caggagcttg caactgccgc gcaatatggc gcgaatgcca ttttcatcgt cgtgaacaac ggcatgtatg gcacgtccgt atgcatcagg agcgcaccta tccggcgcgc atttcgggta cggccctggc caatcccgac tttgcggcgc ttgcgcggac ctatgggctg catggtgaag tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat