BrovA.17737.a

Flagellin protein FlaA

CENTER ID: BrovA.17737.a
ORGANISM: Brucella ovis ATCC 25840
ASSOCIATED DISEASE: Ovine epididymitis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrovA.17737.a.B2.GE38408 Mature protein( BrovA.17737.a ) 18 269
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruOvi136990_1198
BV-BRC: fig|444178.3.peg.1198
RefSeq: YP_001258010.1
UniProt: A0A0H3ATI7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MASILTNSSA LTALQTLAST NKSLESTQNR ISTGLRISEA SDNASYWSIA TSMKSDNKAN SAVQDALGLG AGKVDTAYSA INKIRESVDD IKTKLVSAMG ASTEDKGKIE TEIKSIVANI NSALSNANYA GSNLLNGPTT DLNVVASYNR SGNAVTVDKI TVKATDTDAK TMVKDIVDAG FFTSASDDTA IGTALNTVET ALASLATGAA TLGAAKSQID SQKSFLSALQ VQQQLGVQAL SIATFAQASR ISLRSTGWRW CGCSRISSN
NT Sequence
ATGGCTAGCA TTCTTACAAA CTCGTCGGCT CTGACCGCTC TCCAGACGCT TGCTTCGACC AACAAGTCGC TGGAATCGAC CCAGAACCGT ATCTCGACCG GTCTTCGTAT TTCTGAAGCT TCGGATAATG CTTCGTACTG GTCCATCGCA ACGTCGATGA AGTCCGACAA CAAGGCCAAT TCGGCTGTTC AGGACGCTCT CGGCCTCGGC GCCGGCAAGG TCGATACCGC TTATTCCGCG ATCAACAAGA TCCGTGAATC GGTTGACGAC ATCAAGACCA AGCTTGTTTC GGCTATGGGT GCGAGCACAG AAGACAAGGG CAAGATCGAA ACCGAAATCA AGTCGATCGT CGCTAATATC AACTCGGCTC TCAGCAATGC CAACTACGCG GGTTCCAACC TGCTGAATGG CCCGACGACC GATCTCAATG TTGTAGCTTC GTACAATCGT TCCGGTAACG CCGTCACGGT TGACAAGATC ACTGTCAAGG CTACTGACAC TGATGCGAAG ACCATGGTTA AAGATATCGT AGATGCGGGT TTCTTCACTT CGGCCTCGGA CGATACTGCT ATTGGTACGG CTCTGAATAC GGTTGAAACC GCTCTTGCTT CTCTCGCAAC CGGCGCTGCG ACGCTCGGTG CGGCCAAGTC GCAGATCGAC AGCCAGAAGA GCTTCCTGTC GGCTCTCCAG GTTCAGCAGC AGCTTGGCGT TCAGGCTCTG TCGATCGCCA CTTTCGCGCA AGCTTCGCGC ATTAGCTTGC GTTCAACAGG ATGGCGGTGG TGTGGATGCA GCAGAATTTC CAGCAAT
Details for BrovA.17737.a.B2.GE38408
HARVESTED ON: 8/29/2014
SEQUENCED ON: 12/10/2014
EXPECTED MW: 27kDa
OBSERVED MW: 27kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMA STNKSLESTQ NRISTGLRIS EASDNASYWS IATSMKSDNK ANSAVQDALG LGAGKVDTAY SAINKIRESV DDIKTKLVSA MGASTEDKGK IETEIKSIVA NINSALSNAN YAGSNLLNGP TTDLNVVASY NRSGNAVTVD KITVKATDTD AKTMVKDIVD AGFFTSASDD TAIGTALNTV ETALASLATG AATLGAAKSQ IDSQKSFLSG LQVQQQLGVQ ALSIATFAQA SRISLRSTGW RWCGXXRISS N
Validated NT Sequence
atcttactca cttaattgct ggaaattctg nnncatccac accaccgcca tcctgttgaa cgcaagctaa tgcgcgaagc ttgcgcgaaa gtggcgatcg acagagcctg aacgccaagc tgctgctgaa cctggagacc cgacaggaag ctcttctggc tgtcgatctg cgacttggcc gcaccgagcg tcgcagcgcc ggttgcgaga gaagcaagag cggtttcaac cgtattcaga gccgtaccaa tagcagtatc gtccgaggcc gaagtgaaga aacccgcatc tacgatatct ttaaccatgg tcttcgcatc agtgtcagta gccttgacag tgatcttgtc aaccgtgacg gcgttaccgg aacgattgta cgaagctaca acattgagat cggtcgtcgg gccattcagc aggttggaac ccgcgtagtt ggcattgctg agagccgagt tgatattagc gacgatcgac ttgatttcgg tttcgatctt gcccttgtct tctgtgctcg cacccatagc cgaaacaagc ttggtcttga tgtcgtcaac cgattcacgg atcttgttga tcgcggaata agcggtatcg accttgccgg cgccgaggcc gagagcgtcc tgaacagccg aattggcctt gttgtcggac ttcatcgacg ttgcgatgga ccagtacgaa gcattatccg aagcttcaga aatacgaaga ccggtcgaga tacggttctg ggtcgattcc agcgacttgt tggtcgaagc catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHAS TNKSLESTQN RISTGLRISE ASDNASYWSI ATSMKSDNKA NSAVQDALGL GAGKVDTAYS AINKIRESVD DIKTKLVSAM GASTEDKGKI ETEIKSIVAN INSALSNANY AGSNLLNGPT TDLNVVASYN RSGNAVTVDK ITVKATDTDA KTMVKDIVDA GFFTSASDDT AIGTALNTVE TALASLATGA ATLGAAKSQI DSQKSFLSAL QVQQQLGVQA LSIATFAQAS RISLRSTGWR WCGCSRISSN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgcttcga ccaacaagtc gctggaatcg acccagaacc gtatctcgac cggtcttcgt atttctgaag cttcggataa tgcttcgtac tggtccatcg caacgtcgat gaagtccgac aacaaggcca attcggctgt tcaggacgct ctcggcctcg gcgccggcaa ggtcgatacc gcttattccg cgatcaacaa gatccgtgaa tcggttgacg acatcaagac caagcttgtt tcggctatgg gtgcgagcac agaagacaag ggcaagatcg aaaccgaaat caagtcgatc gtcgctaata tcaactcggc tctcagcaat gccaactacg cgggttccaa cctgctgaat ggcccgacga ccgatctcaa tgttgtagct tcgtacaatc gttccggtaa cgccgtcacg gttgacaaga tcactgtcaa ggctactgac actgatgcga agaccatggt taaagatatc gtagatgcgg gtttcttcac ttcggcctcg gacgatactg ctattggtac ggctctgaat acggttgaaa ccgctcttgc ttctctcgca accggcgctg cgacgctcgg tgcggccaag tcgcagatcg acagccagaa gagcttcctg tcggctctcc aggttcagca gcagcttggc gttcaggctc tgtcgatcgc cactttcgcg caagcttcgc gcattagctt gcgttcaaca ggatggcggt ggtgtggatg cagcagaatt tccagcaatt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat