BrovA.17661.a
Glutamate Aspartate periplasmic binding protein precursor GltI (TC 3.A.1.3.4)
| CENTER ID: | BrovA.17661.a |
| ORGANISM: | Brucella ovis ATCC 25840 |
| ASSOCIATED DISEASE: | Ovine epididymitis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrovA.17661.a.B2.GE38406 | Mature protein( BrovA.17661.a ) | 19 | 338 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrovA.17661.a.B2.PS02300 | Mature protein( BrovA.17661.a ) | 19 | 338 |
Structures
4Z9N
DEPOSITED: 4/10/2015
DETERMINATION: XRay
CLONE: BrovA.17661.a.B2.GE38406
PROTEIN: BrovA.17661.a.B2.PS02300
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|444178.3.peg.2072 |
| RefSeq: | YP_001258723.1 |
| UniProt: | A0A0H3AT66 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTGVLGAAAL FGIASGASAD TLSDVKAKGF LQCGVNTGLL GFASPNDKGE WSGFDVDYCR AVASAIFGDP TKVKFTPLNA KERFTALQSG EVDVLIRNTT WTISRDTSLG LDFAGINYYD GQGFMINSKK LAGINSALQL SGASICVQAG TTTELNMADY FRANKMEYNP VVFEKIEEAN AAYDSGRCDA YTTDQSSLYG VRLALANPDD HVILPEIISK EPFGLTVRQG DARWADVVRW THNALLNAEE YGITQANVEE MKKSDNPDIK RLLGAEADTK IGTDLGLDKD WVVKIIKGVG NYGEIFERNI GSGSPLKIAR GLNAQWNKGG LQYGIPVR
NT Sequence
ATGACGGGTG TATTGGGTGC GGCGGCGCTG TTTGGCATCG CTTCGGGAGC ATCCGCAGAC ACGCTTTCCG ATGTGAAGGC GAAAGGTTTT CTACAGTGCG GCGTAAACAC GGGGCTACTT GGTTTTGCGT CGCCCAATGA TAAGGGTGAA TGGTCCGGTT TCGACGTTGA TTATTGCCGT GCGGTAGCTT CGGCGATCTT CGGTGATCCG ACCAAGGTGA AGTTCACTCC TCTCAATGCA AAAGAGCGTT TCACGGCGCT TCAATCCGGT GAGGTGGACG TTCTGATCCG CAACACGACC TGGACCATCA GCCGCGATAC GTCGCTTGGT CTCGATTTTG CTGGCATCAA CTACTATGAC GGCCAGGGCT TCATGATCAA TTCAAAGAAG CTGGCGGGCA TCAATTCCGC ATTGCAGCTT TCGGGTGCTT CCATCTGCGT CCAGGCCGGC ACGACGACCG AGCTGAACAT GGCCGACTAT TTCCGCGCCA ATAAGATGGA ATATAATCCG GTTGTCTTTG AGAAGATCGA AGAAGCCAAT GCCGCTTATG ATTCCGGCCG TTGTGACGCC TATACGACCG ACCAGTCCAG CCTTTACGGG GTTCGTCTGG CGCTTGCCAA TCCGGACGAT CACGTCATCC TGCCGGAAAT CATTTCCAAG GAGCCTTTCG GCCTGACCGT TCGTCAAGGT GACGCCAGGT GGGCGGATGT CGTTCGCTGG ACGCATAATG CGCTGCTCAA TGCCGAGGAA TACGGCATTA CGCAGGCCAA TGTCGAAGAG ATGAAGAAGT CCGATAATCC GGATATCAAG CGTCTTCTCG GCGCGGAAGC AGACACCAAG ATCGGCACCG ATCTCGGCCT CGACAAGGAC TGGGTGGTGA AGATCATCAA GGGTGTCGGC AATTACGGCG AAATCTTCGA GCGCAACATT GGTTCGGGCA GCCCGCTCAA GATCGCACGC GGCCTCAACG CCCAGTGGAA CAAGGGCGGC CTGCAATATG GCATCCCCGT GCGC
Details for BrovA.17661.a.B2.GE38406
| HARVESTED ON: | 8/29/2014 |
| SEQUENCED ON: | 12/10/2014 |
| EXPECTED MW: | 36kDa |
| OBSERVED MW: | 35kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MAHHHHHHMA DTLSDVKAKG FLQCGVNTGL LGFASPNDKG EWSGFDVDYC RAVASAIFGD PTKVKFTPLN AKERFTALQS GEVDVLIRNT TWTISRDTSL GLDFAGINYY DGQGFMINSK KLAGINSALQ LSGASICVQA GTTTELNMAD YFRANKMEYN PVVFEKIEEA NAAYDSGRCD AYTTDQSSLY GVRLALANPD DHVILPEIIS KEPFGLTVRQ GDARWADVVR WTHNALLNAE EYGITQANVE EMKKSDNPDI KRLLGAEADT KIGTDLGLDK DWVVKIIKGV GNYGEIFERN IGSGSPLKIA RGLNAQWNKG
Validated NT Sequence
gccgcccttg ttccactggg cgttgaggcc gcgtgcgatc ttgagcgggc tgcccgaacc aatgttgcgc tcgaagattt cgccgtaatt gccgacaccc ttgatgatct tcaccaccca gtccttgtcg aggccgagat cggtgccgat cttggtgtct gcttccgcgc cgagaagacg cttgatatcc ggattatcgg acttcttcat ctcttcgaca ttggcctgcg taatgccgta ttcctcggca ttgagcagcg cattatgcgt ccagcgaacg acatccgccc acctggcgtc accttgacga acggtcaggc cgaaaggctc cttggaaatg atttccggca ggatgacgtg atcgtccgga ttggcaagcg ccagacgaac cccgtaaagg ctggactggt cggtcgtata ggcgtcacaa cggccggaat cataagcggc attggcttct tcgatcttct caaagacaac cggattatat tccatcttat tggcgcggaa atagtcggcc atgttcagct cggtcgtcgt gccggcctgg acgcagatgg aagcacccga aagctgcaat gcggaattga tgcccgccag cttctttgaa ttgatcatga agccctggcc gtcatagtag ttgatgccag caaaatcgag accaagcgac gtatcgcggc tgatggtcca ggtcgtgttg cggatcagaa cgtccacctc accggattga agcgccgtga aacgctcttt tgcattgaga ggagtgaact tcaccttggt cggatcaccg aagatcgccg aagctaccgc acggcaataa tcaacgtcga aaccggacca ttcaccctta tcattgggcg acgcaaaacc aagtagcccc gtgtttacgc cgcactgtag aaaacctttc gccttcacat cggaaagcgt gtctgccata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHAD TLSDVKAKGF LQCGVNTGLL GFASPNDKGE WSGFDVDYCR AVASAIFGDP TKVKFTPLNA KERFTALQSG EVDVLIRNTT WTISRDTSLG LDFAGINYYD GQGFMINSKK LAGINSALQL SGASICVQAG TTTELNMADY FRANKMEYNP VVFEKIEEAN AAYDSGRCDA YTTDQSSLYG VRLALANPDD HVILPEIISK EPFGLTVRQG DARWADVVRW THNALLNAEE YGITQANVEE MKKSDNPDIK RLLGAEADTK IGTDLGLDKD WVVKIIKGVG NYGEIFERNI GSGSPLKIAR GLNAQWNKGG LQYGIPVR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgcagaca cgctttccga tgtgaaggcg aaaggttttc tacagtgcgg cgtaaacacg gggctacttg gttttgcgtc gcccaatgat aagggtgaat ggtccggttt cgacgttgat tattgccgtg cggtagcttc ggcgatcttc ggtgatccga ccaaggtgaa gttcactcct ctcaatgcaa aagagcgttt cacggcgctt caatccggtg aggtggacgt tctgatccgc aacacgacct ggaccatcag ccgcgatacg tcgcttggtc tcgattttgc tggcatcaac tactatgacg gccagggctt catgatcaat tcaaagaagc tggcgggcat caattccgca ttgcagcttt cgggtgcttc catctgcgtc caggccggca cgacgaccga gctgaacatg gccgactatt tccgcgccaa taagatggaa tataatccgg ttgtctttga gaagatcgaa gaagccaatg ccgcttatga ttccggccgt tgtgacgcct atacgaccga ccagtccagc ctttacgggg ttcgtctggc gcttgccaat ccggacgatc acgtcatcct gccggaaatc atttccaagg agcctttcgg cctgaccgtt cgtcaaggtg acgccaggtg ggcggatgtc gttcgctgga cgcataatgc gctgctcaat gccgaggaat acggcattac gcaggccaat gtcgaagaga tgaagaagtc cgataatccg gatatcaagc gtcttctcgg cgcggaagca gacaccaaga tcggcaccga tctcggcctc gacaaggact gggtggtgaa gatcatcaag ggtgtcggca attacggcga aatcttcgag cgcaacattg gttcgggcag cccgctcaag atcgcacgcg gcctcaacgc ccagtggaac aagggcggcc tgcaatatgg catccccgtg cgctgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for BrovA.17661.a.B2.PS02300
| PURIFICATION DATe: | 1/15/2015 |
| CONCENTRATION: | 53.52mg/ml |
| OBSERVED MW: | 35kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 5 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMA DTLSDVKAKG FLQCGVNTGL LGFASPNDKG EWSGFDVDYC RAVASAIFGD PTKVKFTPLN AKERFTALQS GEVDVLIRNT TWTISRDTSL GLDFAGINYY DGQGFMINSK KLAGINSALQ LSGASICVQA GTTTELNMAD YFRANKMEYN PVVFEKIEEA NAAYDSGRCD AYTTDQSSLY GVRLALANPD DHVILPEIIS KEPFGLTVRQ GDARWADVVR WTHNALLNAE EYGITQANVE EMKKSDNPDI KRLLGAEADT KIGTDLGLDK DWVVKIIKGV GNYGEIFERN IGSGSPLKIA RGLNAQWNKG
Validated NT Sequence
gccgcccttg ttccactggg cgttgaggcc gcgtgcgatc ttgagcgggc tgcccgaacc aatgttgcgc tcgaagattt cgccgtaatt gccgacaccc ttgatgatct tcaccaccca gtccttgtcg aggccgagat cggtgccgat cttggtgtct gcttccgcgc cgagaagacg cttgatatcc ggattatcgg acttcttcat ctcttcgaca ttggcctgcg taatgccgta ttcctcggca ttgagcagcg cattatgcgt ccagcgaacg acatccgccc acctggcgtc accttgacga acggtcaggc cgaaaggctc cttggaaatg atttccggca ggatgacgtg atcgtccgga ttggcaagcg ccagacgaac cccgtaaagg ctggactggt cggtcgtata ggcgtcacaa cggccggaat cataagcggc attggcttct tcgatcttct caaagacaac cggattatat tccatcttat tggcgcggaa atagtcggcc atgttcagct cggtcgtcgt gccggcctgg acgcagatgg aagcacccga aagctgcaat gcggaattga tgcccgccag cttctttgaa ttgatcatga agccctggcc gtcatagtag ttgatgccag caaaatcgag accaagcgac gtatcgcggc tgatggtcca ggtcgtgttg cggatcagaa cgtccacctc accggattga agcgccgtga aacgctcttt tgcattgaga ggagtgaact tcaccttggt cggatcaccg aagatcgccg aagctaccgc acggcaataa tcaacgtcga aaccggacca ttcaccctta tcattgggcg acgcaaaacc aagtagcccc gtgtttacgc cgcactgtag aaaacctttc gccttcacat cggaaagcgt gtctgccata tggtggtggt ggtggtgagc cat
Expressed Protein Sequence
MAHHHHHHAD TLSDVKAKGF LQCGVNTGLL GFASPNDKGE WSGFDVDYCR AVASAIFGDP TKVKFTPLNA KERFTALQSG EVDVLIRNTT WTISRDTSLG LDFAGINYYD GQGFMINSKK LAGINSALQL SGASICVQAG TTTELNMADY FRANKMEYNP VVFEKIEEAN AAYDSGRCDA YTTDQSSLYG VRLALANPDD HVILPEIISK EPFGLTVRQG DARWADVVRW THNALLNAEE YGITQANVEE MKKSDNPDIK RLLGAEADTK IGTDLGLDKD WVVKIIKGVG NYGEIFERNI GSGSPLKIAR GLNAQWNKGG LQYGIPVR
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGCAGACA CGCTTTCCGA TGTGAAGGCG AAAGGTTTTC TACAGTGCGG CGTAAACACG GGGCTACTTG GTTTTGCGTC GCCCAATGAT AAGGGTGAAT GGTCCGGTTT CGACGTTGAT TATTGCCGTG CGGTAGCTTC GGCGATCTTC GGTGATCCGA CCAAGGTGAA GTTCACTCCT CTCAATGCAA AAGAGCGTTT CACGGCGCTT CAATCCGGTG AGGTGGACGT TCTGATCCGC AACACGACCT GGACCATCAG CCGCGATACG TCGCTTGGTC TCGATTTTGC TGGCATCAAC TACTATGACG GCCAGGGCTT CATGATCAAT TCAAAGAAGC TGGCGGGCAT CAATTCCGCA TTGCAGCTTT CGGGTGCTTC CATCTGCGTC CAGGCCGGCA CGACGACCGA GCTGAACATG GCCGACTATT TCCGCGCCAA TAAGATGGAA TATAATCCGG TTGTCTTTGA GAAGATCGAA GAAGCCAATG CCGCTTATGA TTCCGGCCGT TGTGACGCCT ATACGACCGA CCAGTCCAGC CTTTACGGGG TTCGTCTGGC GCTTGCCAAT CCGGACGATC ACGTCATCCT GCCGGAAATC ATTTCCAAGG AGCCTTTCGG CCTGACCGTT CGTCAAGGTG ACGCCAGGTG GGCGGATGTC GTTCGCTGGA CGCATAATGC GCTGCTCAAT GCCGAGGAAT ACGGCATTAC GCAGGCCAAT GTCGAAGAGA TGAAGAAGTC CGATAATCCG GATATCAAGC GTCTTCTCGG CGCGGAAGCA GACACCAAGA TCGGCACCGA TCTCGGCCTC GACAAGGACT GGGTGGTGAA GATCATCAAG GGTGTCGGCA ATTACGGCGA AATCTTCGAG CGCAACATTG GTTCGGGCAG CCCGCTCAAG ATCGCACGCG GCCTCAACGC CCAGTGGAAC AAGGGCGGCC TGCAATATGG CATCCCCGTG CGCTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT