BrovA.17543.a

Mannose-1-phosphate guanylyltransferase (GDP) ManC (EC 2.7.7.22) / Mannose-6-phosphate isomerase ManC (EC 5.3.1.8)

CENTER ID: BrovA.17543.a
ORGANISM: Brucella ovis ATCC 25840
ASSOCIATED DISEASE: Ovine epididymitis
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrovA.17543.a.B1.GE38404 Full length( BrovA.17543.a ) 1 474
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruOvi136990_1864
BV-BRC: fig|444178.3.peg.1864
RefSeq: YP_001258542.1
UniProt: A0A0H3ANZ2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MREMLMRPVV LAGGKGTRLW PMSRSQRPKQ FLALTGELSL FQMTLKRLSD PNRYSSPIII TNADYRFLVA EQALECGVEL SDVILEPMAR NTAPAIAAAA LIASKDEQTL IHVVASDHNI EINDAYFQAV DTACNAARMG RLATFGITPT EPATGFGYIE AGELEESGAC AIKRFVEKPK EEVAREMLAA GGFYWNSGMF LFRSDVFLDE CRRYAPAVYE AAEASVATAQ RDLDFVRLNS AEFEKAPDIS VDYAIFEKTK LASVVPSPIH WSDLGSWDAV WKESKQDPSG NVARGPVSLH NTNNSLVLSE GVHVAVNGVD DLTIVASEDA LYVGKLSEAQ GVGTIVKALM KQPATAKLAE EHRTIYRPWG GYTHLLSGDR FQVKRLFIKP GKQLSLQKHH HRSEHWVVVR GVAEIVIDDK SLFLSENEST YISQGAIHRV SNPGKILLEL IEVQTGAYLG EDDIVRFEDD FGRV
NT Sequence
GTGCGTGAAA TGTTGATGAG ACCAGTAGTT TTGGCCGGCG GAAAAGGTAC GAGATTGTGG CCGATGTCGC GCTCGCAACG CCCAAAACAG TTTCTGGCTC TGACTGGCGA ACTCAGCCTC TTTCAGATGA CTTTGAAACG ATTGTCGGAC CCGAATCGCT ATTCCTCGCC GATTATCATA ACAAACGCTG ACTATCGGTT TCTGGTGGCG GAACAGGCAT TAGAATGTGG TGTCGAACTT TCGGATGTCA TTCTGGAGCC CATGGCTCGT AACACGGCAC CGGCGATCGC CGCTGCGGCT TTGATCGCTT CCAAGGATGA GCAGACCCTT ATTCATGTTG TGGCATCTGA TCACAATATT GAAATAAACG ATGCATATTT TCAAGCTGTG GATACGGCCT GCAATGCTGC TCGTATGGGA CGTCTTGCCA CTTTTGGCAT TACGCCAACG GAACCAGCGA CCGGTTTTGG TTACATCGAA GCAGGCGAGT TGGAAGAAAG TGGAGCTTGT GCGATTAAGC GCTTTGTGGA AAAACCGAAG GAAGAAGTTG CGCGCGAAAT GCTTGCTGCA GGCGGCTTCT ACTGGAACTC GGGGATGTTT TTGTTCCGTT CGGACGTTTT TCTCGACGAA TGCAGGCGTT ACGCACCAGC AGTATATGAG GCTGCTGAGG CGTCCGTTGC AACTGCACAG CGGGATCTCG ATTTTGTGCG TCTGAACAGC GCCGAATTTG AGAAAGCTCC GGATATTTCC GTTGACTATG CGATTTTCGA GAAGACAAAA CTCGCTTCTG TTGTTCCGTC ACCAATCCAC TGGTCGGATC TTGGATCGTG GGATGCTGTC TGGAAGGAAA GCAAACAGGA TCCGTCGGGA AATGTTGCTC GCGGACCTGT CAGCCTGCAC AACACCAATA ATTCGCTGGT CTTGTCTGAA GGCGTTCATG TTGCGGTCAA TGGCGTTGAC GATCTCACGA TTGTGGCCTC GGAAGATGCG CTTTATGTTG GCAAGCTCTC TGAAGCGCAA GGTGTAGGCA CGATCGTAAA GGCGCTGATG AAACAACCCG CCACGGCGAA GCTTGCTGAG GAGCATCGGA CCATCTATCG CCCTTGGGGC GGCTACACCC ATTTGCTTTC GGGTGATCGG TTTCAGGTAA AGCGTCTGTT CATCAAGCCG GGCAAACAGC TCTCGCTACA GAAGCATCAC CATCGTTCAG AGCATTGGGT GGTTGTTCGG GGCGTGGCCG AAATCGTGAT CGATGATAAG AGCCTTTTCC TGTCGGAGAA CGAATCGACA TATATTTCTC AAGGTGCGAT ACACCGTGTT TCAAATCCGG GTAAAATTCT GCTCGAACTG ATTGAAGTTC AAACTGGAGC TTATCTGGGC GAAGACGATA TTGTGCGCTT CGAGGATGAT TTCGGACGTG TT
Details for BrovA.17543.a.B1.GE38404
HARVESTED ON: 8/29/2014
SEQUENCED ON: 12/10/2014
EXPECTED MW: 53kDa
OBSERVED MW: 53kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMV REMLMRPVVL AGGKGTRLWP MSRSQRPKQF LALTGELSLF QMTLKRLSDP NRYSSPIIIT NADYRFLVAE QALECGVELS DVILEPMARN TAPAIAAAAL IASKDEQTLI HVVASDHNIE INDAYFQAVD TACNAARMGR LATFGITPTE PATGFGYIEA GELEESGACA IKRFVEKPKE EVAREMLAAG GFYWNSGMFL FRSDVFLDEC RRYAPAVYEA AEASVATAQR DLDFVRLNSA EFEKAPDISV DYAIFEKTKL ASVVPSPIHW SDLGSWDAVW KESKQDPSGN VARGPVSLHN TNNSLVLSEG VHVAVNGVDD LTIVASEDAL YVGKLSEAQG VGTIVKALMK QPATAKLAEE HRTIYRPWGG YTHLLSGDRF QVKRLFIKPG KQLSLQKHHH RSEHWVVVRG VAEIVIDDKS LFLSENESTY ISQGAIHRVS NPGKILLELI EVQTGAYLGE DDIV
Validated NT Sequence
gcgcacaata tcgtcttcgc ccagataagc tccagtttga acttcaatca gttcgagcag aattttaccc ggatttgaaa cacggtgtat cgcaccttga gaaatatatg tcgattcgtt ctccgacagg aaaaggctct tatcatcgat cacgatttcg gccacgcccc gaacaaccac ccaatgctct gaacgatggt gatgcttctg tagcgagagc tgtttgcccg gcttgatgaa cagacgcttt acctgaaacc gatcacccga aagcaaatgg gtgtagccgc cccaagggcg atagatggtc cgatgctcct cagcaagctt cgccgtggcg ggttgtttca tcagcgcctt tacgatcgtg cctacacctt gcgcttcaga gagcttgcca acataaagcg catcttccga ggccacaatc gtgagatcgt caacgccatt gaccgcaaca tgaacgcctt cagacaagac cagcgaatta ttggtgttgt gcaggctgac aggtccgcga gcaacatttc ccgacggatc ctgtttgctt tccttccaga cagcatccca cgatccaaga tccgaccagt ggattggtga cggaacaaca gaagcgagtt ttgtcttctc gaaaatcgca tagtcaacgg aaatatccgg agctttctca aattcggcgc tgttcagacg cacaaaatcg agatcccgct gtgcagttgc aacggacgcc tcagcagcct catatactgc tggtgcgtaa cgcctgcatt cgtcgagaaa aacgtccgaa cggaacaaaa acatccccga gttccagtag aagccgcctg cagcaagcat ttcgcgcgca acttcttcct tcggtttttc cacaaagcgc ttaatcgcac aagctccact ttcttccaac tcgcctgctt cgatgtaacc aaaaccggtc gctggttccg ttggcgtaat gccaaaagtg gcaagacgtc ccatacgagc agcattgcag gccgtatcca cagcttgaaa atatgcatcg tttatttcaa tattgtgatc agatgccaca acatgaataa gggtctgctc atccttggaa gcgatcaaag ccgcagcggc gatcgccggt gccgtgttac gagccatggg ctccagaatg acatccgaaa gttcgacacc acattctaat gcctgttccg ccaccagaaa ccgatagtca gcgtttgtta tgataatcgg cgaggaatag cgattcgggt ccgacaatcg tttcaaagtc atctgaaaga ggctgagttc gccagtcaga gccagaaact gttttgggcg ttgcgagcgc gacatcggcc acaatctcgt accttttccg ccggccaaaa ctactggtct catcaacatt tcacgcacca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHMR EMLMRPVVLA GGKGTRLWPM SRSQRPKQFL ALTGELSLFQ MTLKRLSDPN RYSSPIIITN ADYRFLVAEQ ALECGVELSD VILEPMARNT APAIAAAALI ASKDEQTLIH VVASDHNIEI NDAYFQAVDT ACNAARMGRL ATFGITPTEP ATGFGYIEAG ELEESGACAI KRFVEKPKEE VAREMLAAGG FYWNSGMFLF RSDVFLDECR RYAPAVYEAA EASVATAQRD LDFVRLNSAE FEKAPDISVD YAIFEKTKLA SVVPSPIHWS DLGSWDAVWK ESKQDPSGNV ARGPVSLHNT NNSLVLSEGV HVAVNGVDDL TIVASEDALY VGKLSEAQGV GTIVKALMKQ PATAKLAEEH RTIYRPWGGY THLLSGDRFQ VKRLFIKPGK QLSLQKHHHR SEHWVVVRGV AEIVIDDKSL FLSENESTYI SQGAIHRVSN PGKILLELIE VQTGAYLGED DIVRFEDDFG RV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgcgtg aaatgttgat gagaccagta gttttggccg gcggaaaagg tacgagattg tggccgatgt cgcgctcgca acgcccaaaa cagtttctgg ctctgactgg cgaactcagc ctctttcaga tgactttgaa acgattgtcg gacccgaatc gctattcctc gccgattatc ataacaaacg ctgactatcg gtttctggtg gcggaacagg cattagaatg tggtgtcgaa ctttcggatg tcattctgga gcccatggct cgtaacacgg caccggcgat cgccgctgcg gctttgatcg cttccaagga tgagcagacc cttattcatg ttgtggcatc tgatcacaat attgaaataa acgatgcata ttttcaagct gtggatacgg cctgcaatgc tgctcgtatg ggacgtcttg ccacttttgg cattacgcca acggaaccag cgaccggttt tggttacatc gaagcaggcg agttggaaga aagtggagct tgtgcgatta agcgctttgt ggaaaaaccg aaggaagaag ttgcgcgcga aatgcttgct gcaggcggct tctactggaa ctcggggatg tttttgttcc gttcggacgt ttttctcgac gaatgcaggc gttacgcacc agcagtatat gaggctgctg aggcgtccgt tgcaactgca cagcgggatc tcgattttgt gcgtctgaac agcgccgaat ttgagaaagc tccggatatt tccgttgact atgcgatttt cgagaagaca aaactcgctt ctgttgttcc gtcaccaatc cactggtcgg atcttggatc gtgggatgct gtctggaagg aaagcaaaca ggatccgtcg ggaaatgttg ctcgcggacc tgtcagcctg cacaacacca ataattcgct ggtcttgtct gaaggcgttc atgttgcggt caatggcgtt gacgatctca cgattgtggc ctcggaagat gcgctttatg ttggcaagct ctctgaagcg caaggtgtag gcacgatcgt aaaggcgctg atgaaacaac ccgccacggc gaagcttgct gaggagcatc ggaccatcta tcgcccttgg ggcggctaca cccatttgct ttcgggtgat cggtttcagg taaagcgtct gttcatcaag ccgggcaaac agctctcgct acagaagcat caccatcgtt cagagcattg ggtggttgtt cggggcgtgg ccgaaatcgt gatcgatgat aagagccttt tcctgtcgga gaacgaatcg acatatattt ctcaaggtgc gatacaccgt gtttcaaatc cgggtaaaat tctgctcgaa ctgattgaag ttcaaactgg agcttatctg ggcgaagacg atattgtgcg cttcgaggat gatttcggac gtgtttgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat