BrovA.17377.c

Transcriptional regulator, LacI family

CENTER ID: BrovA.17377.c
ORGANISM: Brucella ovis ATCC 25840
ASSOCIATED DISEASE: Ovine epididymitis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrovA.17377.c.B1.GE38395 Full length( BrovA.17377.c ) 1 352
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruOvi136990_3407
BV-BRC: fig|444178.3.peg.3407
RefSeq: YP_001259856.1
UniProt: A0A0H3ANZ7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSKGSVTVID IVREAGVSKS TVSLVLRDSP LVHAETRAKV QEAIEKLGYV YNRSAANLRQ AKSKIIGLVV NDLTNSFFAE LAVGVDRVMQ SAGYVQFLAN TAESIDRQRE VIASMREHGI AGLIVSPARG TEASDLKPLA KSGLPVVQMV RDVPGSGVSS IVSDNRGGVA KVVEHLVSLG HRAIAFMGGY ADIAVFSERL AGYRTGLEQA GIAFDEALVF TSAPSRAGGV EALEQMLRQG MKPTAAVCFN DAVAFGVCDG LRATHLEPGR DFGVVGFDDV IEAKTAVPAL TTVAVDPQGL GERAAQLLLK QINSERVEAE AQRLSVRLAV RASCGAPFRK PEEKFEWQSG AL
NT Sequence
ATGAGCAAGG GCAGCGTCAC CGTTATCGAC ATTGTGCGCG AGGCTGGCGT GTCGAAATCG ACCGTCTCTC TGGTGCTGCG TGACAGTCCG CTGGTCCATG CCGAAACCCG CGCCAAGGTG CAGGAGGCAA TCGAGAAACT GGGCTATGTC TATAACCGCT CGGCGGCCAA TCTCCGGCAG GCGAAATCGA AAATCATCGG CCTTGTGGTC AATGATCTGA CCAACAGTTT CTTTGCCGAA CTGGCGGTGG GTGTGGATCG CGTGATGCAG TCTGCGGGCT ATGTGCAGTT TCTGGCCAAT ACGGCGGAAA GCATTGATCG CCAGCGTGAA GTGATTGCCT CAATGCGCGA ACATGGCATT GCCGGGCTGA TCGTTTCGCC AGCGCGTGGA ACTGAGGCCA GTGATCTGAA ACCATTGGCG AAAAGCGGCC TTCCGGTTGT GCAGATGGTG CGCGATGTGC CGGGTTCGGG TGTTTCCTCC ATCGTTTCGG ATAATCGCGG CGGCGTGGCG AAGGTGGTCG AGCATCTGGT GTCGCTGGGG CATCGCGCCA TCGCTTTCAT GGGTGGTTAT GCGGATATAG CGGTGTTTTC CGAGCGTCTT GCCGGTTATC GCACAGGGTT GGAGCAAGCG GGCATTGCCT TCGACGAGGC GCTGGTTTTC ACCTCTGCGC CGTCACGCGC TGGTGGGGTT GAGGCGCTGG AACAGATGCT GCGGCAGGGC ATGAAACCGA CGGCGGCGGT GTGCTTCAAC GATGCGGTTG CTTTTGGTGT CTGCGATGGC TTGCGAGCCA CGCATCTGGA ACCGGGCCGG GATTTTGGCG TCGTCGGCTT TGACGATGTG ATCGAGGCAA AGACGGCTGT TCCGGCACTG ACGACCGTGG CTGTCGATCC GCAAGGTTTG GGAGAACGTG CGGCGCAGCT TTTGCTGAAA CAAATCAATT CGGAACGGGT GGAGGCGGAG GCGCAGCGTC TGTCGGTGCG TCTTGCAGTG CGTGCAAGTT GCGGCGCGCC GTTCAGAAAA CCGGAGGAGA AATTTGAATG GCAAAGTGGG GCCTTA
Details for BrovA.17377.c.B1.GE38395
HARVESTED ON: 8/29/2014
SEQUENCED ON: 12/10/2014
EXPECTED MW: 38kDa
OBSERVED MW: 38kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHHM SKGSVTVIDI AREAGVSKST VSLVLRDSPL VHAETRAKVQ EAIEKLGYVY NRSAANLRQA KSKIIGLVVN DLTNSFFAEL AVGVDRVMQS AGYVQFLANT AESIDRQREV IASMREHGIA GLIVSPARGT EASDLKPLAK SGLPVVQMVR DVPGSGVSSI VSDNRGGVAK AVEHLVSLGH RAIAFMGGYA DIAVFSERLA GYRTGLEQAG IAFDEALVFT SAPSRAGGVE ALEQMLRQGM KPTAAVCFND AVAFGVCDGL RATHLEPGRD FGVVGFDDVI EAKTAVPALT TVAVDPQGLG ERAAQLLLKQ INSERVEAEA QRLSVRLAVR ASCGAPFRKP EE
Validated NT Sequence
ttctcctccg gttttctgaa cggcgcgccg caacttgcac gcactgcaag acgcaccgac agacgctgcg cctccgcctc cacccgttcc gaattgattt gtttcagcaa aagctgcgcc gcacgttctc ccaaaccttg cggatcgaca gccacggtcg tcagtgccgg aacagccgtc tttgcctcga tcacatcgtc aaagccgacg acgccaaaat cccggcccgg ttccagatgc gtggctcgca agccatcgca gacaccaaaa gcaaccgcat cgttgaagca caccgccgcc gtcggtttca tgccctgccg cagcatctgt tccagcgcct caaccccacc agcgcgtgac ggcgcagagg tgaaaaccag cgcctcgtcg aaggcaatgc ccgcttgctc caaccctgtg cgataaccgg caagacgctc ggaaaacacc gctatatccg cataaccacc catgaaagcg atggcgcgat gccccagcga caccagatgc tcgaccgcct tcgccacgcc gccgcgatta tccgaaacga tggaggaaac acccgaaccc ggcacatcgc gcaccatctg cacaaccgga aggccgcttt tcgccaatgg tttcagatca ctggcctcag ttccacgcgc tggcgaaacg atcagcccgg caatgccatg ttcgcgcatt gaggcaatca cttcacgctg gcgatcaatg ctttccgccg tattggccag aaactgcaca tagcccgcag actgcatcac gcgatccaca cccaccgcca gttcggcaaa gaaactgttg gtcagatcat tgaccacaag gccgatgatt ttcgatttcg cctgccggag attggccgcc gagcggttat agacatagcc cagtttctcg attgcctcct gcaccttggc gcgggtttcg gcatggacca gcggactgtc acgcagcacc agagagacgg tcgatttcga cacgccagcc tcgcgcgcaa tgtcgataac ggtgacgctg cccttgctca tatggtggtg gtggtggtgg tgagccat
Expected Protein Sequence
MAHHHHHHMS KGSVTVIDIV REAGVSKSTV SLVLRDSPLV HAETRAKVQE AIEKLGYVYN RSAANLRQAK SKIIGLVVND LTNSFFAELA VGVDRVMQSA GYVQFLANTA ESIDRQREVI ASMREHGIAG LIVSPARGTE ASDLKPLAKS GLPVVQMVRD VPGSGVSSIV SDNRGGVAKV VEHLVSLGHR AIAFMGGYAD IAVFSERLAG YRTGLEQAGI AFDEALVFTS APSRAGGVEA LEQMLRQGMK PTAAVCFNDA VAFGVCDGLR ATHLEPGRDF GVVGFDDVIE AKTAVPALTT VAVDPQGLGE RAAQLLLKQI NSERVEAEAQ RLSVRLAVRA SCGAPFRKPE EKFEWQSGAL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagca agggcagcgt caccgttatc gacattgtgc gcgaggctgg cgtgtcgaaa tcgaccgtct ctctggtgct gcgtgacagt ccgctggtcc atgccgaaac ccgcgccaag gtgcaggagg caatcgagaa actgggctat gtctataacc gctcggcggc caatctccgg caggcgaaat cgaaaatcat cggccttgtg gtcaatgatc tgaccaacag tttctttgcc gaactggcgg tgggtgtgga tcgcgtgatg cagtctgcgg gctatgtgca gtttctggcc aatacggcgg aaagcattga tcgccagcgt gaagtgattg cctcaatgcg cgaacatggc attgccgggc tgatcgtttc gccagcgcgt ggaactgagg ccagtgatct gaaaccattg gcgaaaagcg gccttccggt tgtgcagatg gtgcgcgatg tgccgggttc gggtgtttcc tccatcgttt cggataatcg cggcggcgtg gcgaaggtgg tcgagcatct ggtgtcgctg gggcatcgcg ccatcgcttt catgggtggt tatgcggata tagcggtgtt ttccgagcgt cttgccggtt atcgcacagg gttggagcaa gcgggcattg ccttcgacga ggcgctggtt ttcacctctg cgccgtcacg cgctggtggg gttgaggcgc tggaacagat gctgcggcag ggcatgaaac cgacggcggc ggtgtgcttc aacgatgcgg ttgcttttgg tgtctgcgat ggcttgcgag ccacgcatct ggaaccgggc cgggattttg gcgtcgtcgg ctttgacgat gtgatcgagg caaagacggc tgttccggca ctgacgaccg tggctgtcga tccgcaaggt ttgggagaac gtgcggcgca gcttttgctg aaacaaatca attcggaacg ggtggaggcg gaggcgcagc gtctgtcggt gcgtcttgca gtgcgtgcaa gttgcggcgc gccgttcaga aaaccggagg agaaatttga atggcaaagt ggggccttat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat