BrovA.17370.a
Leucine-, isoleucine-, valine-, threonine-, and alanine-binding protein
| CENTER ID: | BrovA.17370.a |
| ORGANISM: | Brucella ovis ATCC 25840 |
| ASSOCIATED DISEASE: | Ovine epididymitis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrovA.17370.a.B2.GE38164 | Mature protein( BrovA.17370.a ) | 26 | 416 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrovA.17370.a.B2.PS02137 | Mature protein( BrovA.17370.a ) | 26 | 416 | ||
| BrovA.17370.a.B2.PS02287 | Mature protein( BrovA.17370.a ) | 26 | 416 |
Structures
4XFK
DEPOSITED: 12/27/2014
DETERMINATION: XRay
CLONE: BrovA.17370.a.B2.GE38164
PROTEIN: BrovA.17370.a.B2.PS02137
7JFN
DEPOSITED: 7/17/2020
DETERMINATION: XRay
CLONE: BrovA.17370.a.B2.GE38164
PROTEIN: BrovA.17370.a.B2.PS02137
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|444178.3.peg.25 |
| RefSeq: | YP_001257107.1 |
| UniProt: | A0A0H3ATZ3 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSLKVFLQAG VACAALSLAG AAGASAEPLK IALVETLSGP QASTGLLYRA AVLYQLGKIN EAGGFNGEKI QILEYDNQGG PVGAADRVKA AIADGAQIIV QGSSSAVAGQ ITEDVRKYNL RNKGKEVLYL NLGAEALELT GSKCHFYHFR FSPNAAIHFK TVAQGMKDKG ILGERAYSIN QNYSWGVDVE NTVVANAKEI GYEVVDKTLH EVNKIQDFSP YVAKIQAANV DTVFTGNWSN DLLLLMKAAS GAGLKAKFAT SFLDQPGNIG NAGAIAEGHI VSTPFNPEAN GEASMAFAED YKKVTGHYPS YVEPAAVFGL QLFGEALKNV KPGEGKINTT DIALAIENAS VKTPMGDYSM RSDDHQAKFP MVVQEVSKKA RIKADGTEYG FLPFKTFTGD ESIDPVQESC SMKRPG
NT Sequence
ATGTCCTTAA AAGTTTTCCT GCAGGCCGGT GTTGCCTGTG CAGCCCTGTC GCTGGCCGGA GCGGCTGGTG CCTCAGCCGA ACCGCTGAAG ATCGCACTTG TTGAAACGCT TTCAGGCCCG CAGGCCTCGA CCGGCCTTCT CTATCGTGCG GCGGTGCTTT ACCAGCTTGG CAAGATCAAT GAAGCCGGCG GTTTCAACGG CGAGAAAATC CAGATTCTCG AATATGACAA TCAGGGTGGC CCGGTGGGCG CTGCCGATCG CGTGAAGGCG GCGATTGCCG ATGGCGCGCA GATCATCGTG CAGGGTTCGT CTTCTGCCGT TGCAGGCCAG ATCACCGAGG ATGTGCGCAA ATACAACCTG CGCAACAAGG GCAAGGAAGT CCTTTATCTC AACCTTGGCG CGGAAGCCCT GGAACTGACC GGCTCCAAGT GCCACTTCTA CCATTTCCGC TTCAGCCCCA ATGCCGCGAT CCACTTCAAG ACCGTCGCGC AGGGCATGAA GGACAAGGGC ATTCTGGGCG AGCGCGCCTA TTCCATCAAC CAGAACTATT CCTGGGGCGT GGACGTGGAG AACACGGTTG TCGCCAATGC CAAGGAGATC GGTTACGAAG TCGTCGACAA GACCTTGCAT GAAGTCAACA AAATCCAGGA CTTCTCGCCC TATGTCGCCA AGATTCAGGC CGCCAATGTG GATACGGTCT TCACCGGCAA CTGGTCGAAC GATCTTCTCC TTCTCATGAA GGCTGCCAGC GGCGCGGGCC TCAAGGCCAA GTTCGCCACT TCCTTCCTCG ATCAGCCGGG CAATATCGGC AATGCGGGCG CGATTGCCGA AGGGCATATC GTTTCCACGC CGTTCAACCC GGAGGCCAAT GGCGAAGCCA GCATGGCTTT TGCCGAGGAC TATAAGAAGG TGACGGGCCA CTATCCGTCC TATGTGGAAC CGGCAGCGGT TTTCGGTCTC CAGCTTTTTG GTGAGGCGCT GAAGAACGTC AAGCCCGGCG AGGGCAAGAT CAACACCACG GATATTGCCC TCGCGATCGA AAACGCAAGC GTAAAGACCC CCATGGGCGA TTACAGCATG CGTTCCGACG ACCATCAGGC AAAGTTCCCG ATGGTTGTTC AGGAAGTCTC CAAAAAGGCC AGGATCAAGG CTGACGGTAC GGAATACGGG TTCCTTCCGT TCAAGACTTT CACCGGCGAT GAGTCCATCG ATCCGGTGCA GGAATCCTGC TCCATGAAGC GTCCGGGC
Details for BrovA.17370.a.B2.GE38164
| HARVESTED ON: | 6/11/2014 |
| SEQUENCED ON: | 6/12/2014 |
| EXPECTED MW: | 43kDa |
| OBSERVED MW: | 43kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with sequence variation |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMA EPLKIALVET LSGPQASTGL LYRAAVLYQL GKINEAGGFN GEKIQILEYD NQGGPVGAAD RVKAAIADGA QIIVQGSSSA VAGQITEDVR KYNLRNKGKE VLYLNLGAEA LELTGSKCHF YHFRFSPNAA IRFKTVAQGM KDKGILGERA YSINQNYSWG VDVENTVVAN AKEIGYEVVD KTLHEVNKIQ DFSPYVAKIQ AANVDTVFTG NWSNDLLLLM KAASGAGLKA KFATSFLDQP GNIGNAGAIA EGHIVSTPFN PEANGEASMA FAEDYKKVTG HYPSYVEPAA VFGLQLFGEA LKNVKPGEGK INTTDIALAI ENASVKTPMG DYSMRSDDHQ AKFPMVVQEV SKKARIKADG TEYGFLPFKT FTGDESIDPV QESCSMKRPG
Validated NT Sequence
atggctcacc accaccacca ccatatggcc gaaccgctga agatcgcact tgttgaaacg ctttcaggcc cgcaggcctc gaccggcctt ctctatcgtg cggcggtgct ttaccagctt ggcaagatca atgaagccgg cggtttcaac ggcgagaaaa tccagattct cgaatatgac aatcagggtg gcccggtggg cgctgccgat cgcgtgaagg cggcgattgc cgatggcgcg cagatcatcg tgcagggttc gtcttctgcc gttgcaggcc agatcaccga ggatgtgcgc aaatacaacc tgcgcaacaa gggcaaggaa gtcctttatc tcaaccttgg cgcggaagcc ctggaactga ccggctccaa gtgccacttc taccatttcc gcttcagccc caatgccgcg atccgcttca agaccgtcgc gcagggcatg aaggacaagg gcattctggg cgagcgcgcc tattccatca accagaacta ttcctggggc gtggacgtgg agaacacggt tgtcgccaat gccaaggaga tcggttacga agtcgtcgac aagaccttgc atgaagtcaa caaaatccag gacttctcgc cctatgtcgc caagattcag gccgccaatg tggatacggt cttcaccggc aactggtcga acgatcttct ccttctcatg aaggctgcca gcggcgcggg cctcaaggcc aagttcgcca cttccttcct cgatcagccg ggcaatatcg gcaatgcggg cgcgattgcc gaagggcata tcgtttccac gccgttcaac ccggaggcca atggcgaagc cagcatggct tttgccgagg actataagaa ggtgacgggc cactatccgt cctatgtgga accggcagcg gttttcggtc tccagctttt tggtgaggcg ctgaagaacg tcaagcccgg cgagggcaag atcaacacca cggatattgc cctcgcgatc gaaaacgcaa gcgtaaagac ccccatgggc gattacagca tgcgttccga cgaccatcag gcaaagttcc cgatggttgt tcaggaagtc tccaaaaagg ccaggatcaa ggctgacggt acggaatacg ggttccttcc gttcaagact ttcaccggcg atgagtccat cgatccggtg caggaatcct gctccatgaa gcgtccgggc taa
Expected Protein Sequence
MAHHHHHHAE PLKIALVETL SGPQASTGLL YRAAVLYQLG KINEAGGFNG EKIQILEYDN QGGPVGAADR VKAAIADGAQ IIVQGSSSAV AGQITEDVRK YNLRNKGKEV LYLNLGAEAL ELTGSKCHFY HFRFSPNAAI HFKTVAQGMK DKGILGERAY SINQNYSWGV DVENTVVANA KEIGYEVVDK TLHEVNKIQD FSPYVAKIQA ANVDTVFTGN WSNDLLLLMK AASGAGLKAK FATSFLDQPG NIGNAGAIAE GHIVSTPFNP EANGEASMAF AEDYKKVTGH YPSYVEPAAV FGLQLFGEAL KNVKPGEGKI NTTDIALAIE NASVKTPMGD YSMRSDDHQA KFPMVVQEVS KKARIKADGT EYGFLPFKTF TGDESIDPVQ ESCSMKRPG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgccgaac cgctgaagat cgcacttgtt gaaacgcttt caggcccgca ggcctcgacc ggccttctct atcgtgcggc ggtgctttac cagcttggca agatcaatga agccggcggt ttcaacggcg agaaaatcca gattctcgaa tatgacaatc agggtggccc ggtgggcgct gccgatcgcg tgaaggcggc gattgccgat ggcgcgcaga tcatcgtgca gggttcgtct tctgccgttg caggccagat caccgaggat gtgcgcaaat acaacctgcg caacaagggc aaggaagtcc tttatctcaa ccttggcgcg gaagccctgg aactgaccgg ctccaagtgc cacttctacc atttccgctt cagccccaat gccgcgatcc acttcaagac cgtcgcgcag ggcatgaagg acaagggcat tctgggcgag cgcgcctatt ccatcaacca gaactattcc tggggcgtgg acgtggagaa cacggttgtc gccaatgcca aggagatcgg ttacgaagtc gtcgacaaga ccttgcatga agtcaacaaa atccaggact tctcgcccta tgtcgccaag attcaggccg ccaatgtgga tacggtcttc accggcaact ggtcgaacga tcttctcctt ctcatgaagg ctgccagcgg cgcgggcctc aaggccaagt tcgccacttc cttcctcgat cagccgggca atatcggcaa tgcgggcgcg attgccgaag ggcatatcgt ttccacgccg ttcaacccgg aggccaatgg cgaagccagc atggcttttg ccgaggacta taagaaggtg acgggccact atccgtccta tgtggaaccg gcagcggttt tcggtctcca gctttttggt gaggcgctga agaacgtcaa gcccggcgag ggcaagatca acaccacgga tattgccctc gcgatcgaaa acgcaagcgt aaagaccccc atgggcgatt acagcatgcg ttccgacgac catcaggcaa agttcccgat ggttgttcag gaagtctcca aaaaggccag gatcaaggct gacggtacgg aatacgggtt ccttccgttc aagactttca ccggcgatga gtccatcgat ccggtgcagg aatcctgctc catgaagcgt ccgggctgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for BrovA.17370.a.B2.PS02137
| PURIFICATION DATe: | 8/21/2014 |
| CONCENTRATION: | 49.9mg/ml |
| OBSERVED MW: | 45kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 8 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMA EPLKIALVET LSGPQASTGL LYRAAVLYQL GKINEAGGFN GEKIQILEYD NQGGPVGAAD RVKAAIADGA QIIVQGSSSA VAGQITEDVR KYNLRNKGKE VLYLNLGAEA LELTGSKCHF YHFRFSPNAA IRFKTVAQGM KDKGILGERA YSINQNYSWG VDVENTVVAN AKEIGYEVVD KTLHEVNKIQ DFSPYVAKIQ AANVDTVFTG NWSNDLLLLM KAASGAGLKA KFATSFLDQP GNIGNAGAIA EGHIVSTPFN PEANGEASMA FAEDYKKVTG HYPSYVEPAA VFGLQLFGEA LKNVKPGEGK INTTDIALAI ENASVKTPMG DYSMRSDDHQ AKFPMVVQEV SKKARIKADG TEYGFLPFKT FTGDESIDPV QESCSMKRPG
Validated NT Sequence
atggctcacc accaccacca ccatatggcc gaaccgctga agatcgcact tgttgaaacg ctttcaggcc cgcaggcctc gaccggcctt ctctatcgtg cggcggtgct ttaccagctt ggcaagatca atgaagccgg cggtttcaac ggcgagaaaa tccagattct cgaatatgac aatcagggtg gcccggtggg cgctgccgat cgcgtgaagg cggcgattgc cgatggcgcg cagatcatcg tgcagggttc gtcttctgcc gttgcaggcc agatcaccga ggatgtgcgc aaatacaacc tgcgcaacaa gggcaaggaa gtcctttatc tcaaccttgg cgcggaagcc ctggaactga ccggctccaa gtgccacttc taccatttcc gcttcagccc caatgccgcg atccgcttca agaccgtcgc gcagggcatg aaggacaagg gcattctggg cgagcgcgcc tattccatca accagaacta ttcctggggc gtggacgtgg agaacacggt tgtcgccaat gccaaggaga tcggttacga agtcgtcgac aagaccttgc atgaagtcaa caaaatccag gacttctcgc cctatgtcgc caagattcag gccgccaatg tggatacggt cttcaccggc aactggtcga acgatcttct ccttctcatg aaggctgcca gcggcgcggg cctcaaggcc aagttcgcca cttccttcct cgatcagccg ggcaatatcg gcaatgcggg cgcgattgcc gaagggcata tcgtttccac gccgttcaac ccggaggcca atggcgaagc cagcatggct tttgccgagg actataagaa ggtgacgggc cactatccgt cctatgtgga accggcagcg gttttcggtc tccagctttt tggtgaggcg ctgaagaacg tcaagcccgg cgagggcaag atcaacacca cggatattgc cctcgcgatc gaaaacgcaa gcgtaaagac ccccatgggc gattacagca tgcgttccga cgaccatcag gcaaagttcc cgatggttgt tcaggaagtc tccaaaaagg ccaggatcaa ggctgacggt acggaatacg ggttccttcc gttcaagact ttcaccggcg atgagtccat cgatccggtg caggaatcct gctccatgaa gcgtccgggc taa
Expressed Protein Sequence
MAHHHHHHAE PLKIALVETL SGPQASTGLL YRAAVLYQLG KINEAGGFNG EKIQILEYDN QGGPVGAADR VKAAIADGAQ IIVQGSSSAV AGQITEDVRK YNLRNKGKEV LYLNLGAEAL ELTGSKCHFY HFRFSPNAAI HFKTVAQGMK DKGILGERAY SINQNYSWGV DVENTVVANA KEIGYEVVDK TLHEVNKIQD FSPYVAKIQA ANVDTVFTGN WSNDLLLLMK AASGAGLKAK FATSFLDQPG NIGNAGAIAE GHIVSTPFNP EANGEASMAF AEDYKKVTGH YPSYVEPAAV FGLQLFGEAL KNVKPGEGKI NTTDIALAIE NASVKTPMGD YSMRSDDHQA KFPMVVQEVS KKARIKADGT EYGFLPFKTF TGDESIDPVQ ESCSMKRPG
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGCCGAAC CGCTGAAGAT CGCACTTGTT GAAACGCTTT CAGGCCCGCA GGCCTCGACC GGCCTTCTCT ATCGTGCGGC GGTGCTTTAC CAGCTTGGCA AGATCAATGA AGCCGGCGGT TTCAACGGCG AGAAAATCCA GATTCTCGAA TATGACAATC AGGGTGGCCC GGTGGGCGCT GCCGATCGCG TGAAGGCGGC GATTGCCGAT GGCGCGCAGA TCATCGTGCA GGGTTCGTCT TCTGCCGTTG CAGGCCAGAT CACCGAGGAT GTGCGCAAAT ACAACCTGCG CAACAAGGGC AAGGAAGTCC TTTATCTCAA CCTTGGCGCG GAAGCCCTGG AACTGACCGG CTCCAAGTGC CACTTCTACC ATTTCCGCTT CAGCCCCAAT GCCGCGATCC ACTTCAAGAC CGTCGCGCAG GGCATGAAGG ACAAGGGCAT TCTGGGCGAG CGCGCCTATT CCATCAACCA GAACTATTCC TGGGGCGTGG ACGTGGAGAA CACGGTTGTC GCCAATGCCA AGGAGATCGG TTACGAAGTC GTCGACAAGA CCTTGCATGA AGTCAACAAA ATCCAGGACT TCTCGCCCTA TGTCGCCAAG ATTCAGGCCG CCAATGTGGA TACGGTCTTC ACCGGCAACT GGTCGAACGA TCTTCTCCTT CTCATGAAGG CTGCCAGCGG CGCGGGCCTC AAGGCCAAGT TCGCCACTTC CTTCCTCGAT CAGCCGGGCA ATATCGGCAA TGCGGGCGCG ATTGCCGAAG GGCATATCGT TTCCACGCCG TTCAACCCGG AGGCCAATGG CGAAGCCAGC ATGGCTTTTG CCGAGGACTA TAAGAAGGTG ACGGGCCACT ATCCGTCCTA TGTGGAACCG GCAGCGGTTT TCGGTCTCCA GCTTTTTGGT GAGGCGCTGA AGAACGTCAA GCCCGGCGAG GGCAAGATCA ACACCACGGA TATTGCCCTC GCGATCGAAA ACGCAAGCGT AAAGACCCCC ATGGGCGATT ACAGCATGCG TTCCGACGAC CATCAGGCAA AGTTCCCGAT GGTTGTTCAG GAAGTCTCCA AAAAGGCCAG GATCAAGGCT GACGGTACGG AATACGGGTT CCTTCCGTTC AAGACTTTCA CCGGCGATGA GTCCATCGAT CCGGTGCAGG AATCCTGCTC CATGAAGCGT CCGGGCTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T
Details for BrovA.17370.a.B2.PS02287
| PURIFICATION DATe: | 12/18/2014 |
| CONCENTRATION: | 71mg/ml |
| OBSERVED MW: | 45kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 4 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMA EPLKIALVET LSGPQASTGL LYRAAVLYQL GKINEAGGFN GEKIQILEYD NQGGPVGAAD RVKAAIADGA QIIVQGSSSA VAGQITEDVR KYNLRNKGKE VLYLNLGAEA LELTGSKCHF YHFRFSPNAA IRFKTVAQGM KDKGILGERA YSINQNYSWG VDVENTVVAN AKEIGYEVVD KTLHEVNKIQ DFSPYVAKIQ AANVDTVFTG NWSNDLLLLM KAASGAGLKA KFATSFLDQP GNIGNAGAIA EGHIVSTPFN PEANGEASMA FAEDYKKVTG HYPSYVEPAA VFGLQLFGEA LKNVKPGEGK INTTDIALAI ENASVKTPMG DYSMRSDDHQ AKFPMVVQEV SKKARIKADG TEYGFLPFKT FTGDESIDPV QESCSMKRPG
Validated NT Sequence
atggctcacc accaccacca ccatatggcc gaaccgctga agatcgcact tgttgaaacg ctttcaggcc cgcaggcctc gaccggcctt ctctatcgtg cggcggtgct ttaccagctt ggcaagatca atgaagccgg cggtttcaac ggcgagaaaa tccagattct cgaatatgac aatcagggtg gcccggtggg cgctgccgat cgcgtgaagg cggcgattgc cgatggcgcg cagatcatcg tgcagggttc gtcttctgcc gttgcaggcc agatcaccga ggatgtgcgc aaatacaacc tgcgcaacaa gggcaaggaa gtcctttatc tcaaccttgg cgcggaagcc ctggaactga ccggctccaa gtgccacttc taccatttcc gcttcagccc caatgccgcg atccgcttca agaccgtcgc gcagggcatg aaggacaagg gcattctggg cgagcgcgcc tattccatca accagaacta ttcctggggc gtggacgtgg agaacacggt tgtcgccaat gccaaggaga tcggttacga agtcgtcgac aagaccttgc atgaagtcaa caaaatccag gacttctcgc cctatgtcgc caagattcag gccgccaatg tggatacggt cttcaccggc aactggtcga acgatcttct ccttctcatg aaggctgcca gcggcgcggg cctcaaggcc aagttcgcca cttccttcct cgatcagccg ggcaatatcg gcaatgcggg cgcgattgcc gaagggcata tcgtttccac gccgttcaac ccggaggcca atggcgaagc cagcatggct tttgccgagg actataagaa ggtgacgggc cactatccgt cctatgtgga accggcagcg gttttcggtc tccagctttt tggtgaggcg ctgaagaacg tcaagcccgg cgagggcaag atcaacacca cggatattgc cctcgcgatc gaaaacgcaa gcgtaaagac ccccatgggc gattacagca tgcgttccga cgaccatcag gcaaagttcc cgatggttgt tcaggaagtc tccaaaaagg ccaggatcaa ggctgacggt acggaatacg ggttccttcc gttcaagact ttcaccggcg atgagtccat cgatccggtg caggaatcct gctccatgaa gcgtccgggc taa
Expressed Protein Sequence
MAHHHHHHAE PLKIALVETL SGPQASTGLL YRAAVLYQLG KINEAGGFNG EKIQILEYDN QGGPVGAADR VKAAIADGAQ IIVQGSSSAV AGQITEDVRK YNLRNKGKEV LYLNLGAEAL ELTGSKCHFY HFRFSPNAAI HFKTVAQGMK DKGILGERAY SINQNYSWGV DVENTVVANA KEIGYEVVDK TLHEVNKIQD FSPYVAKIQA ANVDTVFTGN WSNDLLLLMK AASGAGLKAK FATSFLDQPG NIGNAGAIAE GHIVSTPFNP EANGEASMAF AEDYKKVTGH YPSYVEPAAV FGLQLFGEAL KNVKPGEGKI NTTDIALAIE NASVKTPMGD YSMRSDDHQA KFPMVVQEVS KKARIKADGT EYGFLPFKTF TGDESIDPVQ ESCSMKRPG
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGCCGAAC CGCTGAAGAT CGCACTTGTT GAAACGCTTT CAGGCCCGCA GGCCTCGACC GGCCTTCTCT ATCGTGCGGC GGTGCTTTAC CAGCTTGGCA AGATCAATGA AGCCGGCGGT TTCAACGGCG AGAAAATCCA GATTCTCGAA TATGACAATC AGGGTGGCCC GGTGGGCGCT GCCGATCGCG TGAAGGCGGC GATTGCCGAT GGCGCGCAGA TCATCGTGCA GGGTTCGTCT TCTGCCGTTG CAGGCCAGAT CACCGAGGAT GTGCGCAAAT ACAACCTGCG CAACAAGGGC AAGGAAGTCC TTTATCTCAA CCTTGGCGCG GAAGCCCTGG AACTGACCGG CTCCAAGTGC CACTTCTACC ATTTCCGCTT CAGCCCCAAT GCCGCGATCC ACTTCAAGAC CGTCGCGCAG GGCATGAAGG ACAAGGGCAT TCTGGGCGAG CGCGCCTATT CCATCAACCA GAACTATTCC TGGGGCGTGG ACGTGGAGAA CACGGTTGTC GCCAATGCCA AGGAGATCGG TTACGAAGTC GTCGACAAGA CCTTGCATGA AGTCAACAAA ATCCAGGACT TCTCGCCCTA TGTCGCCAAG ATTCAGGCCG CCAATGTGGA TACGGTCTTC ACCGGCAACT GGTCGAACGA TCTTCTCCTT CTCATGAAGG CTGCCAGCGG CGCGGGCCTC AAGGCCAAGT TCGCCACTTC CTTCCTCGAT CAGCCGGGCA ATATCGGCAA TGCGGGCGCG ATTGCCGAAG GGCATATCGT TTCCACGCCG TTCAACCCGG AGGCCAATGG CGAAGCCAGC ATGGCTTTTG CCGAGGACTA TAAGAAGGTG ACGGGCCACT ATCCGTCCTA TGTGGAACCG GCAGCGGTTT TCGGTCTCCA GCTTTTTGGT GAGGCGCTGA AGAACGTCAA GCCCGGCGAG GGCAAGATCA ACACCACGGA TATTGCCCTC GCGATCGAAA ACGCAAGCGT AAAGACCCCC ATGGGCGATT ACAGCATGCG TTCCGACGAC CATCAGGCAA AGTTCCCGAT GGTTGTTCAG GAAGTCTCCA AAAAGGCCAG GATCAAGGCT GACGGTACGG AATACGGGTT CCTTCCGTTC AAGACTTTCA CCGGCGATGA GTCCATCGAT CCGGTGCAGG AATCCTGCTC CATGAAGCGT CCGGGCTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T