SSGCID: Target Page

BrovA.17368.b

High-affinity leucine-specific transport system, periplasmic binding protein LivK (TC 3.A.1.4.1)

CENTER ID:	BrovA.17368.b
ORGANISM:	Brucella ovis ATCC 25840
ASSOCIATED DISEASE:	Ovine epididymitis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrovA.17368.b.B1.GE38163	Full length( BrovA.17368.b )		1	303

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	PATRIC_Locus_Tag:VBIBruOvi136990_3137

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MGDGVKFVIG HFNSGVSIPT SDIYAENGVL MIAPGTTNPT FTERELWNTF RTCHRDDKQG IVAGKYMADN YKDGKVAILH DKTPYGQGLA DETKKSLNEN GMKETLYEGV NQGDKDFSAL ISKMKAAGIT AVYWGGMHPE AGLLIRQMAD QGLKAQFISG DGIVSNELAS IAGDAVAGVM NTFGPDPRDD KANAELIKAF RDKGFEPEAY TFYAYAGVQS LVNAANAAGS NDPMEVATAM KEKGPFKTVL GDISFDAKGD PSLSPYVMFE WARAKTASII TSRSKLPDIL AKNATKFSNL PGF

NT Sequence

GTGGGCGATG GCGTCAAGTT TGTGATTGGC CATTTCAATT CCGGTGTCAG CATTCCGACC TCGGATATCT ATGCCGAAAA CGGCGTGCTG ATGATTGCAC CGGGCACGAC CAACCCGACC TTTACCGAGC GCGAATTGTG GAACACATTC CGCACCTGCC ACCGCGACGA CAAGCAGGGC ATTGTCGCAG GCAAGTACAT GGCCGATAAT TACAAGGACG GCAAGGTTGC CATTCTGCAC GACAAGACGC CCTATGGTCA GGGCCTTGCC GACGAAACTA AAAAGTCGCT CAACGAAAAC GGCATGAAGG AAACGCTCTA TGAGGGCGTG AATCAGGGCG ACAAGGATTT CTCCGCGCTC ATTTCCAAGA TGAAAGCTGC GGGCATCACT GCCGTTTACT GGGGCGGTAT GCACCCGGAA GCTGGCCTTC TGATTCGCCA GATGGCCGAT CAGGGGCTGA AAGCCCAGTT CATTTCGGGT GACGGCATCG TGTCCAACGA GCTGGCGTCG ATTGCCGGTG ACGCCGTGGC CGGTGTGATG AATACTTTCG GTCCCGATCC GCGCGACGAC AAGGCCAATG CCGAACTCAT CAAGGCGTTC CGTGACAAGG GTTTCGAGCC GGAAGCCTAT ACGTTCTATG CCTATGCCGG TGTGCAATCG CTTGTGAACG CGGCCAATGC GGCTGGCAGC AATGATCCGA TGGAAGTCGC TACCGCGATG AAGGAAAAGG GTCCGTTCAA GACCGTGCTC GGCGATATCT CCTTCGATGC GAAGGGTGAT CCGAGCCTGT CGCCCTATGT CATGTTTGAA TGGGCAAGGG CGAAGACGGC AAGTATAATT ACTTCCAGAA GTAAATTGCC TGATATATTA GCAAAAAATG CTACAAAATT CAGCAATCTG CCCGGCTTT

Details for BrovA.17368.b.B1.GE38163

HARVESTED ON:	6/11/2014
SEQUENCED ON:	6/12/2014
EXPECTED MW:	34kDa
OBSERVED MW:	34kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	95
PERCENT COVERAGE:	94

Validated AA Sequence

MAHHHHHHMV GDGVKFVIGH FNSGVSIPTS DIYAENGVLM IAPGTTNPTF TERELWNTFR TCRRDDKQGI VAGKYMADNY KDGKVAILHD KTPYGQGLAD ETKKSLNENG MKETLYEGVN QGDKDFSALI SKMKAAGITA VYWGGMHPEA GLLIRQMADQ GLKAQFISGD GIVSNELASI AGDAVAGVMN TFGPDPRDDK ANAELIKAFR DKGFEPEAYT FYAYAGVQSL VNAANAAGSN DPMEVATAMX EKGPFKTVLG DISFDAKGDP SLSPYVMFEW RKGEDGKYNY FQK

Validated NT Sequence

cnctagatat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtgg gcgatggcgt caagtttgtg attggccatt tcaattccgg tgtcagcatt ccgacctcgg atatctatgc cgaaaacggc gtgctgatga ttgcaccggg cacgaccaac ccgaccttta ccgagcgcga attgtggaac acattccgca cctgccgccg cgacgacaag cagggcattg tcgcaggcaa gtacatggcc gataattaca aggacggcaa ggttgccatt ctgcacgaca agacgcccta tggtcagggc cttgccgacg aaactaaaaa gtcgctcaac gagaacggca tgaaggaaac gctctatgag ggcgtgaatc agggcgacaa ggatttctcc gcgctcattt ccaagatgaa agctgcgggc atcactgccg tttactgggg cggtatgcac ccggaagctg gccttctgat tcgccagatg gccgatcagg ggctgaaagc ccagttcatt tcgggtgacg gcatcgtgtc caacgagctg gcgtcgattg ccggtgacgc cgtggccggt gtgatgaata ctttcggtcc cgatccgcgc gacgacaagg ccaatgccga actcatcaag gcgttccgtg acaagggttt cgagccggaa gcctatacgt tctatgccta tgccggtgtg caatcgcttg tgaacgcggc caatgcggct ggcagcaatg atccgatgga agtcgctacc gcgatgaang aaaagggtcc gttcaagacc gtgctcggcg atatctcctt cgatgcgaag ggtgatccga gcctgtcgcc ctatgtcatg tttgaatggc gcaagggcga agacggcaag tataattact tccagaagta aattgcctga tatattagca aaaaatgcta caaaattcag caatctgccc ggcttttaag tgagtanata natcnnnnnn aanncccccn nnngannnag agaganntnn nnnnaaaaaa aaaatgnncn nggcaagtct tccatcgant tccnnngtan nnntcgcgnt tcnnnannng tctncnagaa agtgtcnnna annnctgnnn nnngnngann nntnnnnnnn nnnnnncnnn nnnnnnnnng nnttnncnng gnnncantnc cnnatnnnnn nnntnnnnnt cannannanc nnnnnactnn angnnntncn ngnnntnnna ttannna

Expected Protein Sequence

MAHHHHHHMG DGVKFVIGHF NSGVSIPTSD IYAENGVLMI APGTTNPTFT ERELWNTFRT CHRDDKQGIV AGKYMADNYK DGKVAILHDK TPYGQGLADE TKKSLNENGM KETLYEGVNQ GDKDFSALIS KMKAAGITAV YWGGMHPEAG LLIRQMADQG LKAQFISGDG IVSNELASIA GDAVAGVMNT FGPDPRDDKA NAELIKAFRD KGFEPEAYTF YAYAGVQSLV NAANAAGSND PMEVATAMKE KGPFKTVLGD ISFDAKGDPS LSPYVMFEWA RAKTASIITS RSKLPDILAK NATKFSNLPG F

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgggcg atggcgtcaa gtttgtgatt ggccatttca attccggtgt cagcattccg acctcggata tctatgccga aaacggcgtg ctgatgattg caccgggcac gaccaacccg acctttaccg agcgcgaatt gtggaacaca ttccgcacct gccaccgcga cgacaagcag ggcattgtcg caggcaagta catggccgat aattacaagg acggcaaggt tgccattctg cacgacaaga cgccctatgg tcagggcctt gccgacgaaa ctaaaaagtc gctcaacgaa aacggcatga aggaaacgct ctatgagggc gtgaatcagg gcgacaagga tttctccgcg ctcatttcca agatgaaagc tgcgggcatc actgccgttt actggggcgg tatgcacccg gaagctggcc ttctgattcg ccagatggcc gatcaggggc tgaaagccca gttcatttcg ggtgacggca tcgtgtccaa cgagctggcg tcgattgccg gtgacgccgt ggccggtgtg atgaatactt tcggtcccga tccgcgcgac gacaaggcca atgccgaact catcaaggcg ttccgtgaca agggtttcga gccggaagcc tatacgttct atgcctatgc cggtgtgcaa tcgcttgtga acgcggccaa tgcggctggc agcaatgatc cgatggaagt cgctaccgcg atgaaggaaa agggtccgtt caagaccgtg ctcggcgata tctccttcga tgcgaagggt gatccgagcc tgtcgcccta tgtcatgttt gaatgggcaa gggcgaagac ggcaagtata attacttcca gaagtaaatt gcctgatata ttagcaaaaa atgctacaaa attcagcaat ctgcccggct tttgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat