BrovA.17356.a

ABC transporter, periplasmic substrate-binding protein

CENTER ID: BrovA.17356.a
ORGANISM: Brucella ovis ATCC 25840
ASSOCIATED DISEASE: Ovine epididymitis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrovA.17356.a.B2.GE38162 Mature protein( BrovA.17356.a ) 29 615
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruOvi136990_0623
BV-BRC: fig|444178.3.peg.623
RefSeq: WP_006643288.1
RefSeq: YP_001257565.1
UniProt: A0A0H3ARP9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLNRFIAFFR SVFLIGLVAT AFGALPARAA NETAPDYALS MHGDVALPAD YTHFPYTNPD APKKGSLTVG VVGTFDSLNP FVLKSMRTTA RGLYNDGEFG NMVYQTLMLR SRDEPFTLYS LLAEKVAIDP ERKWVEFTLN PKAKWSDGQP VTVDDVLFTY DILTEKGRPP YNSRMSRVAK IEKTGERSVR FTFNEKSDRE FPMLIAGSMP VLPKHAINRD TFGNSTLEPP IGSGPYVVAS VQPGQRIVYK RNPDYWGKDL PSQRGFNNFD KISIEYYRNE TSLFESFKKG ILDIFIEGNP IRWEKLYDFP AVEQGKVIKD TFEKGTPADM LGFVFNTRRP IFADRRVRQA LGLLFDFEWA NSNLFAGQYR RTQSFWEGSQ LSSVGRPADA RERELLAPFP GAVREDVMNG TWHPPVTDGS GHDRVPAKKA YDLLSQAGFQ FKDGMAIDPT GKPFAFEIMT RSPDEEKIAL AYQRNLSRLG IAVEIHTVDD AQYQQRLQTF DYDMILGALA SSLSPGNEQW LRWGSASRDV QGSFNFAGVA DPAVDAMIEA LLAARNRADF VSAVRALDRV LISGDYYVPL YHLPYQWVAR WDRIEHPQKT PLSGYQLPAW WHTSQ
NT Sequence
ATGTTGAACA GATTTATTGC TTTTTTCCGC AGTGTGTTTC TCATTGGCCT TGTTGCGACA GCCTTTGGCG CGTTGCCCGC GCGGGCGGCA AACGAGACAG CACCCGACTA TGCACTCTCC ATGCATGGCG ACGTGGCTTT GCCTGCCGAT TATACCCATT TTCCCTACAC CAATCCCGAT GCCCCGAAAA AGGGTTCGCT GACCGTAGGC GTGGTGGGCA CTTTCGACAG CCTTAATCCC TTTGTGCTGA AAAGTATGCG CACCACCGCG CGCGGCCTGT ATAATGATGG CGAGTTTGGC AATATGGTGT ACCAGACGCT GATGCTGCGC TCGCGCGATG AGCCTTTTAC GCTCTATAGT CTGCTGGCTG AGAAAGTTGC CATCGATCCC GAGCGCAAAT GGGTGGAATT CACGCTCAAT CCAAAAGCCA AATGGTCAGA TGGCCAGCCT GTCACGGTGG ACGATGTTCT CTTCACCTAT GACATACTGA CCGAAAAGGG CCGCCCGCCC TATAACAGCC GCATGAGCCG GGTGGCGAAG ATCGAAAAGA CCGGCGAACG CTCGGTGCGC TTCACATTCA ACGAGAAATC CGACCGTGAG TTTCCGATGC TGATCGCAGG CTCGATGCCG GTTCTGCCGA AACATGCCAT CAACCGCGAT ACGTTCGGCA ATTCCACACT GGAACCGCCG ATCGGCAGCG GCCCCTATGT GGTCGCCAGT GTGCAGCCCG GCCAGCGGAT CGTCTACAAG CGCAACCCGG ACTATTGGGG CAAGGATCTG CCCTCGCAGC GCGGCTTCAA CAATTTCGAC AAGATCTCCA TCGAGTATTA CCGCAACGAA ACCTCGCTGT TTGAATCCTT CAAAAAGGGC ATTCTCGACA TTTTCATTGA AGGCAATCCG ATACGCTGGG AAAAGCTCTA TGATTTTCCG GCGGTCGAGC AGGGCAAGGT CATCAAGGAC ACTTTCGAGA AGGGCACACC GGCCGATATG CTGGGCTTCG TGTTCAACAC GCGCCGCCCG ATCTTCGCTG ACCGGCGTGT GCGCCAGGCC CTTGGATTGC TGTTCGATTT TGAGTGGGCG AACAGCAATC TCTTTGCAGG GCAATATCGC CGTACGCAGA GCTTCTGGGA GGGGTCGCAA CTTTCATCCG TCGGCAGGCC CGCCGATGCG CGCGAAAGGG AGTTGCTGGC CCCCTTCCCC GGTGCTGTGC GCGAAGACGT GATGAATGGC ACCTGGCACC CGCCCGTTAC AGATGGCAGC GGCCATGACC GCGTTCCGGC CAAGAAGGCC TATGATCTTC TAAGCCAGGC CGGTTTCCAG TTCAAGGACG GCATGGCCAT AGATCCGACC GGTAAACCGT TTGCCTTCGA GATTATGACG CGATCACCGG ACGAGGAGAA AATCGCGCTC GCCTATCAGC GCAACCTCTC GCGCCTTGGC ATAGCAGTTG AAATCCACAC GGTGGACGAT GCGCAATATC AACAGCGCCT GCAAACCTTC GATTATGATA TGATCCTCGG TGCACTTGCC AGTTCGCTTT CTCCCGGCAA TGAGCAATGG TTGCGGTGGG GTTCCGCCTC GCGCGATGTG CAGGGCAGCT TCAACTTTGC GGGCGTGGCC GACCCTGCCG TTGATGCGAT GATCGAGGCG CTGCTTGCCG CGCGCAACCG CGCCGATTTC GTCAGCGCCG TGCGTGCACT CGACCGTGTG CTGATTTCAG GTGATTATTA TGTTCCGCTC TACCACCTGC CCTATCAATG GGTTGCGCGG TGGGACCGGA TTGAACATCC CCAAAAAACA CCACTTTCCG GCTACCAACT GCCGGCATGG TGGCACACGT CCCAA
Details for BrovA.17356.a.B2.GE38162
HARVESTED ON: 6/11/2014
SEQUENCED ON: 6/12/2014
EXPECTED MW: 67kDa
OBSERVED MW: 67kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHAA NETAPDYALS MHGDVALPAD YTHFPYTNPD APKKGSLTVG VVGTFDSLNP FVLKSMRTTA RGLYNDGEFG NMVYQTLMLR SRDEPFTLYS LLAEKVAIDP ERKWVEFTLN PKAKWSDGQP VTVDDVLFTY DILTEKGRPP YNSRMSRVAK IEKTGERSVR FTFNEKSDRE FPMLIAGSMP VLPKHAINRD TFGNSTLEPP IGSGPYVVAS VQPGQRIVYK RNPDYWGKDL PSQRGFNNFD KISIEYYRNE TSLFESFKKG ILDIFIEGNP IRWEKLYDFP AVEQGKVIKD TFEKGTPADM LGFVFNTRRP IFADRRVRQA LGLLFDFEWA NSNLFAGQYR RTQSFWEGSQ LSSVGRPADA RERELLAPFP GAVREDVMNG TWHPPVTDGS GHDRVPAKKA YDLLSQAGFQ FKDGMAIDPT GKPFAFEIMT RSPDEEKIAL AYQRNLSRLG IAVEIHTVDD AQYQQRLQTF DYDMILGALA SSLSPGNEQW LRWGSASRDV QGSFNFAGVA DPAVDAMIEA LLAARNRADF VSAVRALDRV LISGDYYVPL YHLPYQWVAR WDRIEHPQKT PLSGYQLPAW WHTSQ
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgcggcaa acgagacagc acccgactat gcactctcca tgcatggcga cgtggctttg cctgccgatt atacccattt tccctacacc aatcccgatg ccccgaaaaa gggttcgctg accgtaggcg tggtgggcac tttcgacagc cttaatccct ttgtgctgaa aagtatgcgc accaccgcgc gcggcctgta taatgatggc gagtttggca atatggtgta ccagacgctg atgctgcgct cgcgcgatga gccttttacg ctctatagtc tgctggctga gaaagttgcc atcgatcccg agcgcaaatg ggtggaattc acgctcaatc caaaagccaa atggtcagat ggccagcctg tcacggtgga cgatgttctc ttcacctatg acatactgac cgaaaagggc cgcccgccct ataacagccg catgagccgg gtggcgaaga tcgaaaagac cggcgaacgc tcggtgcgct tcacattcaa cgagaaatcc gaccgtgagt ttccgatgct gatcgcaggc tcgatgccgg ttctgccgaa acatgccatc aaccgcgata cgttcggcaa ttccacactg gaaccgccga tcggcagcgg cccctatgtg gtcgccagtg tgcagcccgg ccagcggatc gtctacaagc gcaacccgga ctattggggc aaggatctgc cctcgcagcg cggcttcaac aatttcgaca agatctccat cgagtattac cgcaacgaaa cctcgctgtt tgaatccttc aaaaagggca ttctcgacat tttcattgaa ggcaatccga tacgctggga aaagctctat gattttccgg cggtcgagca gggcaaggtc atcaaggaca ctttcgagaa gggcacaccg gccgatatgc tgggcttcgt gttcaacacg cgccgcccga tcttcgctga ccggcgtgtg cgccaggccc ttggattgct gttcgatttt gagtgggcga acagcaatct ctttgcaggg caatatcgcc gtacgcagag cttctgggag gggtcgcaac tttcatccgt cggcaggccc gccgatgcgc gcgaaaggga gttgctggcc cccttccccg gtgctgtgcg cgaagacgtg atgaatggca cctggcaccc gcccgttaca gatggcagcg gccatgaccg cgttccggcc aagaaggcct atgatcttct aagccaggcc ggtttccagt tcaaggacgg catggccata gatccgaccg gtaaaccgtt tgccttcgag attatgacgc gatcaccgga cgaggagaaa atcgcgctcg cctatcagcg caacctctcg cgccttggca tagcagttga aatccacacg gtggacgatg cgcaatatca acagcgcctg caaaccttcg attatgatat gatcctcggt gcacttgcca gttcgctttc tcccggcaat gagcaatggt tgcggtgggg ttccgcctcg cgcgatgtgc agggcagctt caactttgcg ggcgtggccg accctgccgt tgatgcgatg atcgaggcgc tgcttgccgc gcgcaaccgc gccgatttcg tcagcgccgt gcgtgcactc gaccgtgtgc tgatttcagg tgattattat gttccgctct accacctgcc ctatcaatgg gttgcgcggt gggaccggat tgaacatccc caaaaaacac cactttccgg ctaccaactg ccggcatggt ggcacacgtc ccaatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat