BrovA.17352.a
Sortase and related acyltransferases family
| CENTER ID: | BrovA.17352.a |
| ORGANISM: | Brucella ovis ATCC 25840 |
| ASSOCIATED DISEASE: | Ovine epididymitis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrovA.17352.a.B1.GE38161 | Full length( BrovA.17352.a ) | 1 | 179 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
5DWM
DEPOSITED: 9/22/2015
DETERMINATION: XRay
CLONE: BrovA.17352.a.B1.GE38161
PROTEIN: BrovA.17352.a.B1.PS02313
5DWN
DEPOSITED: 9/22/2015
DETERMINATION: XRay
CLONE: BrovA.17352.a.B1.GE38161
PROTEIN: BrovA.17352.a.B1.PS02313
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|444178.3.peg.1358 |
| RefSeq: | WP_006155257.1 |
| RefSeq: | YP_001258130.1 |
| UniProt: | A0A0H3AQB6 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPVIRDFQPA DIETITAIYT QAVLTGTGSY EIEPPTMDEM AKRFAAFADQ SFPILVAEAD GRVLGYAYAS YFRVRPAYRW LAEDSIYIAP DAKGQGIGKL LLRELIARIS ALGFRQLLAV IGDGEHNIGS VKLHESLGFT HCGRIEGSGF KHGRWLDTVL MQLPLNGGRS TEPGPSPLS
NT Sequence
ATGCCCGTCA TTCGTGATTT CCAGCCTGCC GATATCGAAA CCATCACCGC CATCTATACG CAAGCCGTGC TGACCGGCAC CGGCTCCTAT GAGATCGAGC CGCCGACAAT GGACGAAATG GCAAAGCGTT TTGCGGCATT TGCGGACCAG AGCTTTCCGA TCCTCGTCGC GGAGGCGGAT GGCCGGGTTC TGGGCTATGC CTATGCGAGC TATTTCCGCG TCAGGCCAGC CTATCGCTGG CTTGCCGAGG ATTCGATCTA TATCGCCCCC GATGCGAAGG GGCAGGGCAT TGGCAAGCTT CTGCTGCGCG AACTCATCGC GCGGATTTCC GCCCTTGGGT TTCGCCAGTT GCTGGCAGTG ATCGGCGACG GCGAACACAA TATTGGCTCG GTGAAGCTGC ATGAAAGCCT GGGCTTTACC CATTGCGGAC GCATCGAGGG TTCGGGCTTC AAGCACGGAC GCTGGCTGGA CACTGTGCTG ATGCAATTGC CGCTCAATGG CGGGCGTTCA ACGGAACCGG GACCATCGCC GCTTTCA
Details for BrovA.17352.a.B1.GE38161
| HARVESTED ON: | 6/11/2014 |
| SEQUENCED ON: | 6/12/2014 |
| EXPECTED MW: | 21kDa |
| OBSERVED MW: | 19kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with sequence variation |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHHM PVIRDFQPAD IETITAIYTQ AVLTGTGSYE IEPPTMDEMA KRFAAFADQG FPILVAEADG RVLGYAYASY FRVRPAYRWL AEDSIYIAPD AKGQGIGKLL LRELIARISA LGFRQLLAVI GDGEHNIGSV KLHESLGFTH CGRIEGSGFK HGRWLDTVLM QLPLNGGRST EPGPSPLS
Validated NT Sequence
atggctcacc accaccacca ccaccatatg cccgtcattc gtgatttcca gcctgccga tatcgaaacc atcaccgcca tctatacgca agccgtgctg a ccggcacc ggctcctatg agatcgagcc gccgacaatg gacgaaatgg ca aagcgtt ttgcggcatt tgcggaccag ggctttccga tcctcgtcgc gga ggcgga tggccgggtt ctgggctatg cctatgcgag ctatttccgc gtca ggcca gcctatcgct ggcttgccga ggattcgatc tatatcgccc ccgat gcga aggggcaggg cattggcaag cttctgctgc gcgaactcat cgcgcg gat ttccgccctt gggtttcgcc agttgctggc agtgatcggc gacggcg aa cacaatattg gctcggtgaa gctgcatgaa agcctgggct ttacccat t gcggacgcat cgagggttcg ggcttcaagc acggacgctg gctggacac tgtgctgatg caattgccgc tcaatggcgg gcgttcaacg gaaccgggac catcgccgc tttcataa
Expected Protein Sequence
MAHHHHHHMP VIRDFQPADI ETITAIYTQA VLTGTGSYEI EPPTMDEMAK RFAAFADQSF PILVAEADGR VLGYAYASYF RVRPAYRWLA EDSIYIAPDA KGQGIGKLLL RELIARISAL GFRQLLAVIG DGEHNIGSVK LHESLGFTHC GRIEGSGFKH GRWLDTVLMQ LPLNGGRSTE PGPSPLS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcccg tcattcgtga tttccagcct gccgatatcg aaaccatcac cgccatctat acgcaagccg tgctgaccgg caccggctcc tatgagatcg agccgccgac aatggacgaa atggcaaagc gttttgcggc atttgcggac cagagctttc cgatcctcgt cgcggaggcg gatggccggg ttctgggcta tgcctatgcg agctatttcc gcgtcaggcc agcctatcgc tggcttgccg aggattcgat ctatatcgcc cccgatgcga aggggcaggg cattggcaag cttctgctgc gcgaactcat cgcgcggatt tccgcccttg ggtttcgcca gttgctggca gtgatcggcg acggcgaaca caatattggc tcggtgaagc tgcatgaaag cctgggcttt acccattgcg gacgcatcga gggttcgggc ttcaagcacg gacgctggct ggacactgtg ctgatgcaat tgccgctcaa tggcgggcgt tcaacggaac cgggaccatc gccgctttca tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat