BrovA.17285.a

Bacterial extracellular solute-binding protein

CENTER ID: BrovA.17285.a
ORGANISM: Brucella ovis ATCC 25840
ASSOCIATED DISEASE: Ovine epididymitis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrovA.17285.a.B1.GE38152 Full length( BrovA.17285.a ) 1 155
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruOvi136990_2293
BV-BRC: fig|444178.3.peg.2293
RefSeq: YP_001258923.1
UniProt: A0A0H3APD8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAAGVANAEE PLKLTIGTEG AYPPFNFIKP DGTLSGFDVD IARALCDQMK AKCEFVTQDW DGAIPALQSG KFDAFIASMS ITDKRKKQVD FTNKYYNTPP GIAAPKDTDI KGVTKEDLAG KTIGSRRFSR CDDAPDPPAR KTARSGETQA WDRGA
NT Sequence
ATGGCTGCGG GCGTCGCCAA TGCAGAAGAA CCGCTCAAGC TCACAATCGG CACCGAAGGC GCCTATCCTC CTTTCAATTT CATCAAGCCG GATGGCACGC TATCGGGCTT CGACGTTGAC ATTGCCCGCG CGCTCTGTGA CCAAATGAAA GCCAAGTGCG AGTTCGTGAC CCAGGACTGG GATGGCGCAA TTCCAGCACT TCAGTCCGGC AAGTTCGATG CCTTCATCGC CTCCATGTCC ATCACGGACA AGCGCAAGAA GCAGGTGGAC TTCACCAATA AATATTACAA TACGCCACCC GGCATCGCCG CTCCGAAGGA TACGGATATC AAGGGCGTAA CCAAGGAAGA CCTTGCAGGC AAGACCATCG GTAGCCGGCG ATTTTCGCGA TGTGATGATG CACCTGATCC GCCTGCCCGC AAAACAGCGC GATCCGGCGA AACTCAAGCA TGGGATCGAG GGGCT
Details for BrovA.17285.a.B1.GE38152
HARVESTED ON: 6/11/2014
SEQUENCED ON: 6/12/2014
EXPECTED MW: 18kDa
OBSERVED MW: 17kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMA AGVANAEEPL KLTIGTEGAY PPFNFIKPDG TLSGFDVDIA RALCDQMKAK CEFVTQDWDG AIPALQSGKF DAFIASMSIT DKRKKQVDFT NKYYNTPPGI AAPKDTDIKG VTKEDLAGKT IGSRRFSRCD DAPDPPARKT ARSGETQAWD RGA
Validated NT Sequence
tccnctagat aattttgttt aactttaaga aggagatata ccatggctca ccaccaccac caccatatgg ctgcgggcgt cgccaatgca gaagaaccgc tcaagctcac aatcggcacc gaaggcgcct atcctccttt caatttcatc aagccggatg gcacgctatc gggcttcgac gttgacattg cccgcgcgct ctgtgaccaa atgaaagcca agtgcgagtt cgtgacccag gactgggatg gcgcaattcc agcacttcag tccggcaagt tcgatgcctt catcgcctcc atgtccatca cggacaagcg caagaagcag gtggacttca ccaataaata ttacaatacg ccacccggca tcgccgctcc gaaggatacg gatatcaagg gcgtaaccaa ggaagacctt gcaggcaaga ccatcggtag ccggcgattt tcgcgatgtg atgatgcacc tgatccgcct gcccgcaaaa cagcgcgatc cggcgaaact caagcatggg atcgaggggc ttaagtgagt aagataggat ccggctgcta acagnccccc caaagagann nngnggaaan aaaannnncn ttannaagtc gtcgaacaac aacgcttgaa tgaaccnaca agtccaggac ttctagccct atgtcgccta gatacacagc ccaccaaagt gganacggtc ttctccgcta actgctcgaa tgattatgtc ctattcatgc aggctgncan ctgcgcgngn nacaaggcca anttcatcag ttccttctgc gatcagtcac nnatatctnn anttcctgct tccattgcng aannnatatc gttccactcg nctcaactcg tacggccaan gacnaatcna ncatgngctn tgacgaanac aatgagacag ggactggtca atactanatc natgtgctac cggcannagn ttatcggtct gcngactttt cgtgacgtag tgtantacnt ctatccgnnn gnngtaanat cnnnnccact gcatntgnnc gtaatgcatg aaaantnact cgtaatnann nntannnnan tacttgcatg actttcnnng atcaattnnn nnntnnnttc ncnnangnnt nntnnngnaa nnnnnnaaaa nnnnggnnnn nncgngnnnn ggngnngnga nacnnnnnnc ncttcnnga
Expected Protein Sequence
MAHHHHHHMA AGVANAEEPL KLTIGTEGAY PPFNFIKPDG TLSGFDVDIA RALCDQMKAK CEFVTQDWDG AIPALQSGKF DAFIASMSIT DKRKKQVDFT NKYYNTPPGI AAPKDTDIKG VTKEDLAGKT IGSRRFSRCD DAPDPPARKT ARSGETQAWD RGA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggctg cgggcgtcgc caatgcagaa gaaccgctca agctcacaat cggcaccgaa ggcgcctatc ctcctttcaa tttcatcaag ccggatggca cgctatcggg cttcgacgtt gacattgccc gcgcgctctg tgaccaaatg aaagccaagt gcgagttcgt gacccaggac tgggatggcg caattccagc acttcagtcc ggcaagttcg atgccttcat cgcctccatg tccatcacgg acaagcgcaa gaagcaggtg gacttcacca ataaatatta caatacgcca cccggcatcg ccgctccgaa ggatacggat atcaagggcg taaccaagga agaccttgca ggcaagacca tcggtagccg gcgattttcg cgatgtgatg atgcacctga tccgcctgcc cgcaaaacag cgcgatccgg cgaaactcaa gcatgggatc gaggggcttg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat