SSGCID: Target Page

BrovA.17284.a

Dipeptide-binding ABC transporter, periplasmic substrate-binding component (TC 3.A.1.5.2)

CENTER ID:	BrovA.17284.a
ORGANISM:	Brucella ovis ATCC 25840
ASSOCIATED DISEASE:	Ovine epididymitis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrovA.17284.a.B2.GE38151	Mature protein( BrovA.17284.a )		27	536

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrovA.17284.a.B2.PS02236	Mature protein( BrovA.17284.a )		27	536

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	PATRIC_Locus_Tag:VBIBruOvi136990_0585
BV-BRC:	fig\|444178.3.peg.585
RefSeq:	YP_001257533.1
UniProt:	A0A0H3AUW7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKGNNMRKFA LLAALSAVLL AGTSAYAAEE PRSGGVINFV APYGDSFSTL DIQASPATQD EFYAKAIHRT LYDWDADQNK PVLGLATDVT VSDDRLVYTY KLRQDAFFHN GKPLTADDII WSYTRIMDPK KAFPPARYIA EIKGAEEYTQ GKAKEISGLK KIDDHTLEIT LKQPIDPGFQ FMHNNTAIYP AGEGDSEEFQ RHPIGLGPYK FAEYVPGSRL TVEKWDKYYE KGKPYADKIN IMIMGDAAAR DVAFRNKEID VAVLGSAQYM AYLADPELAK NMIEVAEVYT RAVGFNPDFK PFQDKRVRQA INYAIDSDLI IKRLVKDKAY RAVSWLPNSS PAFDKDAKPY PYDPEKAKAL LAEAGYPDGF EFELTATQNE SWGLTIVQAI IPMLAKVGIK VKAKPVEASV LADVVPAGNF QAYMWSLESG PDALTAMQCF YSTTPQSACN YQKFSNAEFD KIVDAAKLAK TEEEKNELLK KANNLLQEEA PVWFFNYNKA VMAHQPWLHG LQPNSAELAI QSYEKLWVDD TVPSGR

NT Sequence

ATGAAGGGGA ACAATATGAG AAAATTTGCT TTGCTGGCTG CATTGTCTGC AGTTCTGTTG GCAGGAACTT CAGCTTACGC TGCGGAGGAA CCGCGTTCGG GAGGAGTCAT CAATTTTGTT GCACCTTATG GCGACAGCTT CTCGACGCTC GATATTCAGG CTTCACCCGC CACACAGGAC GAGTTCTACG CAAAGGCTAT TCACCGCACA CTCTATGATT GGGACGCAGA CCAGAACAAG CCGGTGCTTG GTCTTGCGAC TGACGTGACT GTGTCCGACG ATAGACTCGT TTATACTTAT AAGCTTCGTC AGGATGCATT TTTCCACAAT GGCAAGCCTT TGACAGCCGA CGACATAATC TGGAGTTACA CCCGGATAAT GGACCCGAAA AAGGCATTTC CACCAGCACG TTATATTGCT GAAATTAAGG GCGCCGAGGA ATACACGCAG GGTAAGGCTA AGGAAATTTC CGGTCTGAAG AAGATCGATG ACCATACCCT CGAAATCACG CTAAAGCAGC CGATTGATCC GGGCTTCCAG TTCATGCACA ACAACACTGC CATTTATCCG GCTGGTGAGG GAGACAGCGA GGAGTTTCAG CGCCATCCTA TCGGTCTCGG ACCCTATAAA TTTGCGGAAT ATGTACCTGG TTCCCGTCTG ACAGTCGAGA AGTGGGACAA ATATTATGAG AAGGGCAAGC CATACGCCGA CAAGATCAAC ATCATGATTA TGGGCGATGC TGCCGCACGC GACGTTGCGT TCCGTAACAA GGAAATTGAT GTGGCGGTAC TCGGGTCGGC GCAGTACATG GCTTATCTTG CCGATCCAGA ACTTGCCAAG AACATGATCG AAGTGGCCGA GGTTTATACG CGTGCCGTAG GTTTTAACCC TGATTTCAAG CCATTCCAGG ATAAGCGCGT CCGTCAGGCC ATCAACTACG CCATCGATAG CGATCTTATC ATCAAGCGCC TCGTCAAAGA CAAGGCTTAT CGTGCCGTCA GTTGGCTTCC AAACTCATCA CCGGCCTTCG ACAAAGACGC GAAGCCTTAT CCCTATGACC CTGAAAAGGC AAAAGCATTG CTCGCTGAAG CCGGTTATCC CGACGGTTTC GAGTTCGAAT TAACGGCAAC GCAGAACGAA AGCTGGGGCT TAACTATCGT TCAGGCGATT ATTCCGATGC TCGCCAAGGT GGGTATCAAG GTAAAGGCAA AACCTGTTGA AGCATCTGTT CTAGCCGACG TAGTTCCGGC TGGTAATTTC CAGGCCTATA TGTGGTCGCT CGAAAGCGGT CCCGATGCGC TGACGGCAAT GCAGTGCTTC TATTCCACGA CGCCACAATC TGCGTGTAAC TATCAGAAGT TCTCCAATGC TGAATTCGAC AAGATTGTCG ATGCAGCAAA ATTAGCCAAA ACTGAAGAAG AGAAAAACGA ACTTTTAAAG AAGGCAAACA ATCTTTTGCA GGAAGAAGCA CCTGTTTGGT TCTTCAACTA TAACAAGGCT GTCATGGCAC ATCAGCCATG GTTGCATGGT CTACAGCCAA ATTCGGCGGA GCTCGCAATA CAGTCCTATG AAAAGCTGTG GGTAGACGAC ACGGTTCCAT CCGGTCGC

Details for BrovA.17284.a.B2.GE38151

HARVESTED ON:	6/11/2014
SEQUENCED ON:	6/12/2014
EXPECTED MW:	58kDa
OBSERVED MW:	58kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	84
PERCENT COVERAGE:	66

Validated AA Sequence

MAHHHHHHMA AEEPRSGGVI NFVAPYGDSF STLDIQASPA TQDEFYAKAI HRTLYDWDAD QNKPVLGLAT DVTVSDDRLV YTYKLRQDAF FHNGKPLTAD DIIWSYTRIM DPKKAFPPAR YIAEIKGAEE YTQGKAKEIS GLKKIDDHTL EITLKQPIDP GFQFMHNNTA IYPAGEGDSE EFQRHPIGLG PYKFAEYVPG SRLTVEKWDK YYEKGKPYAD KINIMIMGDA AARDVAFRNK EIDVAVLGSA QYMAYLADPE LAKNMIEVAE VYTRAVVLTL ISSHSRISAS VRPSTTPSIA ILSSSASSKT GLSCRQLASK LITGLRQRRE AXPYDPERQK HAR

Validated NT Sequence

gnnnnnncnc tananntttt gtttaacttt aagaaggaga tataccatgg ctcaccacca ccaccaccat atggctgcgg aggaaccgcg ttcgggagga gtcatcaatt ttgttgcacc ttatggcgac agcttctcga cgctcgatat tcaggcttca cccgccacac aggacgagtt ctacgcaaag gctattcacc gcacactcta tgattgggac gcagaccaga acaagccggt gcttggtctt gcgactgacg tgactgtgtc cgacgataga ctcgtttata cttataagct tcgtcaggat gcatttttcc acaatggcaa gcctttgaca gccgacgaca taatctggag ttacacccgg ataatggacc cgaaaaaggc atttccacca gcacgttata ttgctgaaat taagggcgcc gaggaataca cgcagggtaa ggctaaggaa atttccggtc tgaagaagat cgatgaccat accctcgaaa tcacgctaaa gcagccgatt gatccgggct tccagttcat gcacaacaac actgccattt atccggctgg tgagggagac agcgaggagt ttcagcgcca tcctatcggt ctcggaccct ataaatttgc ggaatatgta cctggttccc gtctgacagt cgagaagtgg gacaaatatt atgagaaggg caagccatac gccgacaaga tcaacatcat gattatgggc gatgctgccg cacgcgacgt tgcgttccgt aacaaggaaa ttgatgtggc ggtactcggg tcggcgcagt acatggctta tcttgccgat ccagaacttg ccaagaacat gatcgaagtg gccgaggttt atacgcgtgc cgtagtttta accctgattt caagccattc caggataagc gcgtccgtca ggccatcaac tacgccatcg atagcgatct tatcatcaag cgcctcgtca aagacaggct tatcgtgccg tcagttggct tccaaactca tcaccggcct tcgacaaaga cgcgaagcct ntccctatga ccctgaaagg caaaagcatg ctcgctgagc gnntcccgan nntcnantng annngnnacg canacgaagc tgggnntnac tatcntnang nnnnatnnat gctnnnnagn ggnntcannn nnnannnngt nagnantgnn nancganntg nnnnnnnnnn nnnnggnnnn annntnnngg tg

Expected Protein Sequence

MAHHHHHHAA EEPRSGGVIN FVAPYGDSFS TLDIQASPAT QDEFYAKAIH RTLYDWDADQ NKPVLGLATD VTVSDDRLVY TYKLRQDAFF HNGKPLTADD IIWSYTRIMD PKKAFPPARY IAEIKGAEEY TQGKAKEISG LKKIDDHTLE ITLKQPIDPG FQFMHNNTAI YPAGEGDSEE FQRHPIGLGP YKFAEYVPGS RLTVEKWDKY YEKGKPYADK INIMIMGDAA ARDVAFRNKE IDVAVLGSAQ YMAYLADPEL AKNMIEVAEV YTRAVGFNPD FKPFQDKRVR QAINYAIDSD LIIKRLVKDK AYRAVSWLPN SSPAFDKDAK PYPYDPEKAK ALLAEAGYPD GFEFELTATQ NESWGLTIVQ AIIPMLAKVG IKVKAKPVEA SVLADVVPAG NFQAYMWSLE SGPDALTAMQ CFYSTTPQSA CNYQKFSNAE FDKIVDAAKL AKTEEEKNEL LKKANNLLQE EAPVWFFNYN KAVMAHQPWL HGLQPNSAEL AIQSYEKLWV DDTVPSGR

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgctgcgg aggaaccgcg ttcgggagga gtcatcaatt ttgttgcacc ttatggcgac agcttctcga cgctcgatat tcaggcttca cccgccacac aggacgagtt ctacgcaaag gctattcacc gcacactcta tgattgggac gcagaccaga acaagccggt gcttggtctt gcgactgacg tgactgtgtc cgacgataga ctcgtttata cttataagct tcgtcaggat gcatttttcc acaatggcaa gcctttgaca gccgacgaca taatctggag ttacacccgg ataatggacc cgaaaaaggc atttccacca gcacgttata ttgctgaaat taagggcgcc gaggaataca cgcagggtaa ggctaaggaa atttccggtc tgaagaagat cgatgaccat accctcgaaa tcacgctaaa gcagccgatt gatccgggct tccagttcat gcacaacaac actgccattt atccggctgg tgagggagac agcgaggagt ttcagcgcca tcctatcggt ctcggaccct ataaatttgc ggaatatgta cctggttccc gtctgacagt cgagaagtgg gacaaatatt atgagaaggg caagccatac gccgacaaga tcaacatcat gattatgggc gatgctgccg cacgcgacgt tgcgttccgt aacaaggaaa ttgatgtggc ggtactcggg tcggcgcagt acatggctta tcttgccgat ccagaacttg ccaagaacat gatcgaagtg gccgaggttt atacgcgtgc cgtaggtttt aaccctgatt tcaagccatt ccaggataag cgcgtccgtc aggccatcaa ctacgccatc gatagcgatc ttatcatcaa gcgcctcgtc aaagacaagg cttatcgtgc cgtcagttgg cttccaaact catcaccggc cttcgacaaa gacgcgaagc cttatcccta tgaccctgaa aaggcaaaag cattgctcgc tgaagccggt tatcccgacg gtttcgagtt cgaattaacg gcaacgcaga acgaaagctg gggcttaact atcgttcagg cgattattcc gatgctcgcc aaggtgggta tcaaggtaaa ggcaaaacct gttgaagcat ctgttctagc cgacgtagtt ccggctggta atttccaggc ctatatgtgg tcgctcgaaa gcggtcccga tgcgctgacg gcaatgcagt gcttctattc cacgacgcca caatctgcgt gtaactatca gaagttctcc aatgctgaat tcgacaagat tgtcgatgca gcaaaattag ccaaaactga agaagagaaa aacgaacttt taaagaaggc aaacaatctt ttgcaggaag aagcacctgt ttggttcttc aactataaca aggctgtcat ggcacatcag ccatggttgc atggtctaca gccaaattcg gcggagctcg caatacagtc ctatgaaaag ctgtgggtag acgacacggt tccatccggt cgctgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat

Details for BrovA.17284.a.B2.PS02236

PURIFICATION DATe:	12/2/2014
CONCENTRATION:	53.3mg/ml
OBSERVED MW:	54kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	3
VIAL VOLUME:	110µl
PERCENT IDENTITY:	84
PERCENT COVERAGE:	66

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGCTGCGG AGGAACCGCG TTCGGGAGGA GTCATCAATT TTGTTGCACC TTATGGCGAC AGCTTCTCGA CGCTCGATAT TCAGGCTTCA CCCGCCACAC AGGACGAGTT CTACGCAAAG GCTATTCACC GCACACTCTA TGATTGGGAC GCAGACCAGA ACAAGCCGGT GCTTGGTCTT GCGACTGACG TGACTGTGTC CGACGATAGA CTCGTTTATA CTTATAAGCT TCGTCAGGAT GCATTTTTCC ACAATGGCAA GCCTTTGACA GCCGACGACA TAATCTGGAG TTACACCCGG ATAATGGACC CGAAAAAGGC ATTTCCACCA GCACGTTATA TTGCTGAAAT TAAGGGCGCC GAGGAATACA CGCAGGGTAA GGCTAAGGAA ATTTCCGGTC TGAAGAAGAT CGATGACCAT ACCCTCGAAA TCACGCTAAA GCAGCCGATT GATCCGGGCT TCCAGTTCAT GCACAACAAC ACTGCCATTT ATCCGGCTGG TGAGGGAGAC AGCGAGGAGT TTCAGCGCCA TCCTATCGGT CTCGGACCCT ATAAATTTGC GGAATATGTA CCTGGTTCCC GTCTGACAGT CGAGAAGTGG GACAAATATT ATGAGAAGGG CAAGCCATAC GCCGACAAGA TCAACATCAT GATTATGGGC GATGCTGCCG CACGCGACGT TGCGTTCCGT AACAAGGAAA TTGATGTGGC GGTACTCGGG TCGGCGCAGT ACATGGCTTA TCTTGCCGAT CCAGAACTTG CCAAGAACAT GATCGAAGTG GCCGAGGTTT ATACGCGTGC CGTAGGTTTT AACCCTGATT TCAAGCCATT CCAGGATAAG CGCGTCCGTC AGGCCATCAA CTACGCCATC GATAGCGATC TTATCATCAA GCGCCTCGTC AAAGACAAGG CTTATCGTGC CGTCAGTTGG CTTCCAAACT CATCACCGGC CTTCGACAAA GACGCGAAGC CTTATCCCTA TGACCCTGAA AAGGCAAAAG CATTGCTCGC TGAAGCCGGT TATCCCGACG GTTTCGAGTT CGAATTAACG GCAACGCAGA ACGAAAGCTG GGGCTTAACT ATCGTTCAGG CGATTATTCC GATGCTCGCC AAGGTGGGTA TCAAGGTAAA GGCAAAACCT GTTGAAGCAT CTGTTCTAGC CGACGTAGTT CCGGCTGGTA ATTTCCAGGC CTATATGTGG TCGCTCGAAA GCGGTCCCGA TGCGCTGACG GCAATGCAGT GCTTCTATTC CACGACGCCA CAATCTGCGT GTAACTATCA GAAGTTCTCC AATGCTGAAT TCGACAAGAT TGTCGATGCA GCAAAATTAG CCAAAACTGA AGAAGAGAAA AACGAACTTT TAAAGAAGGC AAACAATCTT TTGCAGGAAG AAGCACCTGT TTGGTTCTTC AACTATAACA AGGCTGTCAT GGCACATCAG CCATGGTTGC ATGGTCTACA GCCAAATTCG GCGGAGCTCG CAATACAGTC CTATGAAAAG CTGTGGGTAG ACGACACGGT TCCATCCGGT CGCTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT