BrovA.17239.a

4-hydroxy-3-methylbut-2-enyl diphosphate reductase (EC 1.17.1.2)

CENTER ID: BrovA.17239.a
ORGANISM: Brucella ovis ATCC 25840
ASSOCIATED DISEASE: Ovine epididymitis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrovA.17239.a.B1.GE38150 Full length( BrovA.17239.a ) 1 372
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruOvi136990_1796
BV-BRC: fig|444178.3.peg.1796
RefSeq: WP_011950121.1
RefSeq: YP_001258492.1
UniProt: A0A0H3AQE6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEKSRKSHDR SVLRFLRHLL ENEYAIMTQQ RPPLEIRLCG PRGFCAGVDR AIQIVVLALK KYGAPVYVRH EIVHNRYVVE GLQARGAIFV EELDEIPAAH RNQPVVFSAH GVPKSVPADA EAKNLFYLDA TCPLVSKVHK QAMRHQRLGR HVILIGHSGH PEVIGTMGQL PDGAVTLIET VEDAHTCHFD DEDNLGFVTQ TTLSVDDTAG IIKELQARFP NLAAPAAESI CYATTNRQDA VRAAAPGCDL FLIVGAPNSS NSKRLVEVAE KAGARMPMLV QRAEDIEWEQ IGDISVVGLS AGASAPEIIV DEIIDAFKAR FDVKIELAET TVETENFLVN REIRDVELTV KDMAFVNGEH RVVGISKLMQ GK
NT Sequence
ATGGAAAAAA GCCGTAAATC GCATGACAGG TCCGTCCTGC GTTTTTTGCG TCATCTACTG GAAAACGAGT ACGCCATAAT GACCCAACAG CGCCCCCCTC TGGAGATCCG CCTTTGCGGC CCGCGCGGCT TTTGCGCAGG TGTGGACCGG GCCATCCAGA TTGTCGTCCT GGCGCTCAAA AAATATGGTG CGCCGGTTTA TGTGCGCCAT GAGATCGTGC ACAATCGTTA TGTGGTCGAG GGGCTTCAGG CCCGCGGCGC GATCTTCGTC GAGGAACTGG ATGAAATCCC GGCGGCACAC CGCAACCAGC CGGTGGTGTT TTCCGCCCAT GGTGTGCCAA AATCCGTGCC AGCGGACGCG GAGGCCAAGA ACCTCTTTTA TCTTGATGCG ACATGCCCAC TGGTTTCCAA GGTGCACAAA CAGGCCATGC GCCACCAGCG CCTTGGCCGC CATGTCATCC TGATCGGCCA TTCCGGCCAC CCGGAAGTGA TCGGCACGAT GGGGCAATTG CCGGATGGCG CGGTCACGCT GATTGAAACC GTCGAGGATG CGCACACCTG CCATTTCGAT GATGAAGACA ATCTTGGCTT CGTCACGCAG ACCACGCTGT CGGTGGATGA CACGGCGGGT ATCATCAAGG AATTGCAGGC GCGCTTTCCC AATCTGGCGG CTCCGGCGGC GGAATCGATC TGCTACGCCA CCACCAACCG GCAGGATGCT GTGCGTGCGG CGGCGCCGGG CTGCGATCTG TTTCTGATTG TCGGTGCACC GAATTCCTCC AATTCCAAGC GTCTGGTCGA GGTGGCGGAA AAGGCGGGCG CGCGCATGCC CATGCTGGTG CAGCGCGCGG AGGATATAGA ATGGGAGCAG ATCGGCGATA TTTCCGTCGT TGGCCTTTCG GCTGGCGCGT CTGCGCCCGA AATCATCGTG GATGAAATCA TCGATGCCTT CAAAGCCCGC TTCGATGTGA AGATCGAACT GGCGGAAACA ACGGTTGAAA CCGAAAATTT CCTTGTGAAT CGCGAAATCC GCGATGTGGA ATTGACGGTG AAGGATATGG CATTCGTCAA TGGCGAGCAC CGCGTGGTCG GCATTTCCAA ACTTATGCAG GGGAAG
Details for BrovA.17239.a.B1.GE38150
HARVESTED ON: 6/11/2014
SEQUENCED ON: 6/13/2014
EXPECTED MW: 42kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 96
PERCENT COVERAGE: 88
Validated AA Sequence
MAHHHHHHME KSRKSHDRSV LRFLRHLLEN EYAIMTQQRP PLEIRLCGPR GFCAGVDRAI QIVVLALKKY GAPVYVRHEI VHNRYVVEGL QARGAIFVEE LDEIPAAHRN QPVVFSAHGV PKSVPADAEA KNLFYLDATC PLVSKVHKQA MRHQRLGRHV ILIGHSGHPE VIGTMGQLPD GAVTLIETVE DAHTCHFDDE DNLGFVTQTT LSVDDTAGII KELQARFPNL AAPAAESICY ATTNRQDAVR AAAPGCDLFL IVGAPNSSXS KRLVEVAEKA GARMSMLVQR AEDIEWEQIG DISVVGLSAG ASAPEIIVDE IIDAFKLTSX XXRLXEXX
Validated NT Sequence
ctanatattt tgtttaactt taagaaggag atataccatg gctcaccacc accaccacca tatggaaaaa agccgtaaat cgcatgacag gtccgtcctg cgttttttgc gtcatctact ggaaaacgag tacgccataa tgacccaaca gcgcccccct ctggagatcc gcctttgcgg cccgcgcggc ttttgcgcag gtgtggaccg ggccatccag attgtcgtcc tggcgctcaa aaaatatggt gcgccggttt atgtgcgcca tgagatcgtg cacaatcgtt atgtggtcga ggggcttcag gcccgcggcg cgatcttcgt cgaggaactg gatgaaatcc cggcggcaca ccgcaaccag ccggtggtgt tttccgccca tggtgtgcca aaatccgtgc cagcggacgc ggaggccaag aacctctttt atcttgatgc gacatgccca ctggtttcca aggtgcacaa acaggccatg cgccaccagc gccttggccg ccatgtcatc ctgatcggcc attccggcca cccggaagtg atcggcacga tggggcaatt gccggatggc gcggtcacgc tgattgaaac cgtcgaggat gcgcacacct gccatttcga tgatgaagac aatcttggct tcgtcacgca gaccacgctg tcggtggatg acacggcggg tatcatcaag gaattgcagg cgcgctttcc caatctggcg gctccggcgg cggaatcgat ctgctacgcc accaccaacc ggcaggatgc tgtgcgtgcg gcggcgccgg gctgcgatct gtttctgatt gtcggtgcac cgaattcctc cnattccaag cgtctggtcg aggtggcgga aaaggcgggc gcgcgcatgt ccatgctggt gcagcgcgcg gaggatatag aatgggagca gatcggcgat atttccgtcg ttggcctttc ggctggcgcg tctgcgcccg aaatcatcgt ggatgaaatc atcgatgcct tcaagctcac ttcgangtna natcgactgg nggaannnnn ntgaaaccga aaatttcctt gnnnatcgcn nanccgnnat gnnnnntgac ggnnannnaa nngnantcgt catggcnnnn ncnnngnnnn acttgcnnnn tatgnntnnn nntnnnnnnn nnnnnnang
Expected Protein Sequence
MAHHHHHHME KSRKSHDRSV LRFLRHLLEN EYAIMTQQRP PLEIRLCGPR GFCAGVDRAI QIVVLALKKY GAPVYVRHEI VHNRYVVEGL QARGAIFVEE LDEIPAAHRN QPVVFSAHGV PKSVPADAEA KNLFYLDATC PLVSKVHKQA MRHQRLGRHV ILIGHSGHPE VIGTMGQLPD GAVTLIETVE DAHTCHFDDE DNLGFVTQTT LSVDDTAGII KELQARFPNL AAPAAESICY ATTNRQDAVR AAAPGCDLFL IVGAPNSSNS KRLVEVAEKA GARMPMLVQR AEDIEWEQIG DISVVGLSAG ASAPEIIVDE IIDAFKARFD VKIELAETTV ETENFLVNRE IRDVELTVKD MAFVNGEHRV VGISKLMQGK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaaa aaagccgtaa atcgcatgac aggtccgtcc tgcgtttttt gcgtcatcta ctggaaaacg agtacgccat aatgacccaa cagcgccccc ctctggagat ccgcctttgc ggcccgcgcg gcttttgcgc aggtgtggac cgggccatcc agattgtcgt cctggcgctc aaaaaatatg gtgcgccggt ttatgtgcgc catgagatcg tgcacaatcg ttatgtggtc gaggggcttc aggcccgcgg cgcgatcttc gtcgaggaac tggatgaaat cccggcggca caccgcaacc agccggtggt gttttccgcc catggtgtgc caaaatccgt gccagcggac gcggaggcca agaacctctt ttatcttgat gcgacatgcc cactggtttc caaggtgcac aaacaggcca tgcgccacca gcgccttggc cgccatgtca tcctgatcgg ccattccggc cacccggaag tgatcggcac gatggggcaa ttgccggatg gcgcggtcac gctgattgaa accgtcgagg atgcgcacac ctgccatttc gatgatgaag acaatcttgg cttcgtcacg cagaccacgc tgtcggtgga tgacacggcg ggtatcatca aggaattgca ggcgcgcttt cccaatctgg cggctccggc ggcggaatcg atctgctacg ccaccaccaa ccggcaggat gctgtgcgtg cggcggcgcc gggctgcgat ctgtttctga ttgtcggtgc accgaattcc tccaattcca agcgtctggt cgaggtggcg gaaaaggcgg gcgcgcgcat gcccatgctg gtgcagcgcg cggaggatat agaatgggag cagatcggcg atatttccgt cgttggcctt tcggctggcg cgtctgcgcc cgaaatcatc gtggatgaaa tcatcgatgc cttcaaagcc cgcttcgatg tgaagatcga actggcggaa acaacggttg aaaccgaaaa tttccttgtg aatcgcgaaa tccgcgatgt ggaattgacg gtgaaggata tggcattcgt caatggcgag caccgcgtgg tcggcatttc caaacttatg caggggaagt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat