BrovA.12797.a

ROK family protein

CENTER ID: BrovA.12797.a
ORGANISM: Brucella ovis ATCC 25840
ASSOCIATED DISEASE: Ovine epididymitis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrovA.12797.a.B1.GE38147 Full length( BrovA.12797.a ) 1 334
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruOvi136990_1251
BV-BRC: fig|444178.3.peg.1251
RefSeq: YP_001258036.1
UniProt: A0A0H3AUK5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRLVPSAHYV AGVYIGAEAV QVAICDLTAQ LVTSAAFDHA ANDNPLKLTH QTADVLRNLV QAKGISLGSL VGIGMGVPGP VDQVRRRNLL SINTGWRDVA FADAMEAELN IPTVVEHNVT AMALAEAHYG IGQGCPAVLY VYLGTGLVVD GMPFRPGGHG AVELGHIQID PQGALCACGN RGCLETFVSE RVLRERGAGS AEPLLAALAR NPTLHDEVAG HFTTALANAV NLLTPDLIVL GGHFAEAPEA FYARLRCDLP PRVLPHMRDV LRIERGGFGN NAGAVDHLFY SEYPDECTPD GPGPQSSFLC RGDRWLVSPH GHGGGGAARP GGCA
NT Sequence
GTGCGTCTGG TTCCCTCGGC TCATTATGTG GCTGGCGTGT ATATTGGCGC CGAAGCTGTG CAGGTGGCGA TTTGCGATCT GACGGCGCAA CTGGTCACCA GCGCTGCTTT CGATCATGCC GCAAACGATA ATCCGCTGAA ACTGACGCAT CAGACAGCCG ATGTTCTGCG CAATCTCGTG CAGGCCAAAG GCATTTCATT GGGTAGCCTC GTCGGTATCG GCATGGGGGT GCCAGGACCG GTCGATCAGG TGCGCCGCCG CAATTTGCTG TCAATCAATA CCGGCTGGCG CGATGTCGCT TTTGCCGATG CAATGGAAGC GGAACTGAAT ATTCCAACTG TGGTTGAGCA TAATGTTACA GCCATGGCGC TTGCCGAGGC CCATTACGGC ATAGGGCAGG GCTGCCCGGC TGTTCTTTAT GTCTATCTTG GTACGGGGCT GGTGGTGGAC GGCATGCCGT TTCGCCCCGG CGGTCATGGT GCGGTGGAGC TAGGACATAT CCAGATCGAT CCGCAAGGTG CGCTTTGCGC CTGCGGCAAT CGCGGCTGTC TGGAGACCTT TGTGTCGGAA CGCGTGTTGC GCGAGCGTGG CGCTGGCAGC GCAGAGCCGC TTCTTGCGGC GCTTGCCCGT AACCCGACAT TGCATGATGA AGTGGCAGGC CACTTTACAA CTGCGCTCGC CAATGCGGTC AATCTGCTGA CACCGGATTT GATCGTGCTC GGGGGGCATT TTGCGGAAGC GCCAGAAGCT TTCTACGCGC GTTTGCGGTG CGATCTGCCG CCACGGGTGC TGCCGCATAT GCGCGATGTC TTGCGGATAG AGCGCGGCGG TTTCGGGAAC AATGCTGGCG CAGTGGACCA CCTGTTTTAT TCGGAGTATC CCGATGAATG CACGCCTGAC GGGCCTGGGC CTCAATCTTC TTTCCTTTGC CGTGGGGATC GGTGGCTGGT ATCTCCTCAC GGCCACGGGG GCGGTGGTGC TGCCCGGCCC GGTGGATGTG CT
Details for BrovA.12797.a.B1.GE38147
HARVESTED ON: 6/11/2014
SEQUENCED ON: 6/12/2014
EXPECTED MW: 36kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 98
PERCENT COVERAGE: 92
Validated AA Sequence
MAHHHHHHMV RLVPSAHYVA GVHIGAEAVQ VAICDLTAQL VTSAAFDHAA NDNPLKLTHQ TADVLRNLVQ AKGISLGSLV GIGMGVPGPV DQVRRRNLLS INTGWRDVAF ADAMEAELNI PTVVEHNVTA MALAEAHYGI GQGCPAVLYV YLGTGLGAGL VVDGMPFRPG GHGAVELGHI QIDPQGALCA CGNRGCLETF VSERVLRERG AGSAEPLLAA LARNPALHDE VAGHFTTALA NAVNLLTPDL IVLGGHFAEE PEAFYARLRC DLPPRVLPHM RDVLRIERGG FGNNAGAVGA AAVALDHLFY SGVSR
Validated NT Sequence
annncnctan annttttgtt taactttaag aaggagatat accatggctc accaccacca ccaccatatg gtgcgtctgg ttccctcggc tcattatgtg gctggcgtgc atattggcgc agaagctgtg caggtggcga tttgcgatct gacggcgcaa ctggtcacca gcgctgcttt cgatcatgcc gcaaacgata atccgctgaa actgacgcat cagacagccg atgttctgcg caatctcgtg caggccaaag gcatttcatt gggtagcctc gtcggtatcg gcatgggggt gccaggaccg gtcgatcagg tgcgccgccg caatttgctg tcaatcaata ccggctggcg cgatgtcgct tttgccgatg caatggaagc ggaactgaat attccaactg tggttgagca taatgttaca gccatggcgc ttgccgaggc ccattacggc atagggcagg gctgcccggc tgttctttat gtctatcttg gtacggggct gggagcgggg ctggtggtgg acggcatgcc gtttcgcccc ggcggtcatg gtgcggtgga gctaggacat atccagatcg atccgcaagg tgcgctttgc gcctgcggca atcgcggctg tctggagacc tttgtgtcgg aacgcgtgtt gcgcgagcgt ggcgctggca gcgcagagcc gcttcttgcg gcgctcgccc gtaacccggc attgcatgat gaagtggcag gccactttac aactgcgctc gccaatgcgg tcaatctgct gacaccggat ttgatcgtgc tcggggggca ttttgcggaa gagccagaag ctttctacgc gcgtttgcgg tgcgatctgc cgccacgggt gctgccgcat atgcgcgatg tcttgcggat agagcgcggc ggtttcggga acaatgctgg cgcagtgggc gcggctgccg tcgcactgga ccacctgttt tattcgggag tatcccgatg aatgcacgcc tgacgggcct gggcctcaat cttctttcct ttgccgtggg gatcggtggc tggtatcnnc cttcacggcc acgggggccg nnggtgctgc ccggcccgnn ntgtgcnnnt gagtaagann natcgnnnnn nnnnnnnnga nnagngnnnn nnnnnnngag annag
Expected Protein Sequence
MAHHHHHHMR LVPSAHYVAG VYIGAEAVQV AICDLTAQLV TSAAFDHAAN DNPLKLTHQT ADVLRNLVQA KGISLGSLVG IGMGVPGPVD QVRRRNLLSI NTGWRDVAFA DAMEAELNIP TVVEHNVTAM ALAEAHYGIG QGCPAVLYVY LGTGLVVDGM PFRPGGHGAV ELGHIQIDPQ GALCACGNRG CLETFVSERV LRERGAGSAE PLLAALARNP TLHDEVAGHF TTALANAVNL LTPDLIVLGG HFAEAPEAFY ARLRCDLPPR VLPHMRDVLR IERGGFGNNA GAVDHLFYSE YPDECTPDGP GPQSSFLCRG DRWLVSPHGH GGGGAARPGG CA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgcgtc tggttccctc ggctcattat gtggctggcg tgtatattgg cgccgaagct gtgcaggtgg cgatttgcga tctgacggcg caactggtca ccagcgctgc tttcgatcat gccgcaaacg ataatccgct gaaactgacg catcagacag ccgatgttct gcgcaatctc gtgcaggcca aaggcatttc attgggtagc ctcgtcggta tcggcatggg ggtgccagga ccggtcgatc aggtgcgccg ccgcaatttg ctgtcaatca ataccggctg gcgcgatgtc gcttttgccg atgcaatgga agcggaactg aatattccaa ctgtggttga gcataatgtt acagccatgg cgcttgccga ggcccattac ggcatagggc agggctgccc ggctgttctt tatgtctatc ttggtacggg gctggtggtg gacggcatgc cgtttcgccc cggcggtcat ggtgcggtgg agctaggaca tatccagatc gatccgcaag gtgcgctttg cgcctgcggc aatcgcggct gtctggagac ctttgtgtcg gaacgcgtgt tgcgcgagcg tggcgctggc agcgcagagc cgcttcttgc ggcgcttgcc cgtaacccga cattgcatga tgaagtggca ggccacttta caactgcgct cgccaatgcg gtcaatctgc tgacaccgga tttgatcgtg ctcggggggc attttgcgga agcgccagaa gctttctacg cgcgtttgcg gtgcgatctg ccgccacggg tgctgccgca tatgcgcgat gtcttgcgga tagagcgcgg cggtttcggg aacaatgctg gcgcagtgga ccacctgttt tattcggagt atcccgatga atgcacgcct gacgggcctg ggcctcaatc ttctttcctt tgccgtgggg atcggtggct ggtatctcct cacggccacg ggggcggtgg tgctgcccgg cccggtggat gtgcttgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat