BrovA.01365.c
putative oxoacyl-(acyl carrier protein) reductase
| CENTER ID: | BrovA.01365.c |
| ORGANISM: | Brucella ovis ATCC 25840 |
| ASSOCIATED DISEASE: | Ovine epididymitis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrovA.01365.c.B1.GE38071 | Full length( BrovA.01365.c ) | 1 | 246 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
4Z0T
DEPOSITED: 3/26/2015
DETERMINATION: XRay
CLONE: BrovA.01365.c.B1.GE38071
PROTEIN: BrovA.01365.c.B1.PS02315
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | PATRIC_Locus_Tag:VBIBruOvi136990_1767 |
| BV-BRC: | fig|444178.3.peg.1767 |
| RefSeq: | YP_001258465.1 |
| UniProt: | A0A0H3ALP1 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNIDLTGRLA LVTGASRGIG YFLSLELAKR GAHVIAVART VGGLEELDDE IRKLGSSATL VPLDITDMEA LDRLGGTIHE RWGKLDILVA NAGILGTISP IGHVEAKTFE KVMNINVTSV WRLIRSVDPL LRASDAGRAI MLSSGVAHSC RAFWGPYAAS KAAVEVMARC WAEETKKMKL KINSVNPGAT RTAMRAQAMP GEDPETLPTP QSVAEKIVKL AYPKLEVTGK LFDVRQDRFL DYHMPS
NT Sequence
ATGAATATCG ATCTTACGGG CCGCCTCGCG CTGGTAACGG GCGCATCGCG CGGTATTGGC TATTTCCTTT CGCTTGAACT GGCAAAACGC GGCGCGCATG TGATCGCCGT CGCCCGCACG GTTGGCGGCC TGGAAGAGCT GGACGACGAA ATACGCAAGC TTGGCAGCAG CGCCACACTG GTTCCACTCG ATATCACGGA TATGGAAGCC CTCGACCGCC TCGGCGGAAC CATCCATGAA CGCTGGGGCA AGCTTGATAT TCTGGTGGCC AATGCCGGCA TATTGGGCAC GATTTCGCCC ATTGGCCATG TCGAGGCCAA AACCTTCGAG AAGGTCATGA ATATCAATGT CACCAGCGTC TGGCGCCTGA TCCGTTCGGT CGATCCGCTG CTGCGCGCCT CGGATGCCGG GCGGGCGATC ATGCTCTCCT CCGGTGTGGC GCATAGCTGC CGCGCTTTCT GGGGGCCTTA TGCCGCCTCC AAGGCCGCCG TCGAGGTTAT GGCGCGGTGC TGGGCCGAAG AAACCAAAAA GATGAAGCTC AAGATCAATT CGGTCAATCC GGGCGCAACC CGCACCGCCA TGCGCGCGCA GGCCATGCCG GGCGAAGACC CGGAAACGCT CCCCACGCCG CAATCGGTCG CCGAAAAGAT CGTCAAGCTT GCCTATCCGA AACTTGAAGT GACGGGCAAG CTTTTCGACG TGCGCCAGGA CCGCTTCCTC GACTATCACA TGCCAAGC
Details for BrovA.01365.c.B1.GE38071
| HARVESTED ON: | 5/13/2014 |
| SEQUENCED ON: | 5/6/2014 |
| EXPECTED MW: | 28kDa |
| OBSERVED MW: | 27kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with sequence variation |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMN IDLTGRLALV TGASRGIGYF LSLELAKRGA HVIAVARTVG GLEELDDEIR KLGSSATLVP LDITDMEALD RLGGTIHERW GKLDILVANA GILGTISPIG HVEAKTFEKV MNINVTSVWR LIRSVDPLLR ASDAGRAIML SSGVAHSCRA FWGPYAASKA AVEVMARCWA EETKKMKLKI NSVNPGATRT AMRAQAMPGE DPETLPTPQS VAEKIVKLAD PKLEVTGKLF DVRQDRFLDY HMPS
Validated NT Sequence
atggctcacc accaccacca ccatatgaat atcgatctta cgggccgcct cgcgctggta acgggcgcat cgcgcggtat tggctatttc ctttcgcttg aactggcaaa acgcggcgcg catgtgatcg ccgtcgcccg cacggttggc ggcctggaag agctggacga cgaaatacgc aagcttggca gcagcgccac actggttcca ctcgatatca cggatatgga agccctcgac cgcctcggcg gaaccatcca tgaacgctgg ggcaagcttg atattctggt ggccaatgcc ggcatattgg gcacgatttc gcccattggc catgtcgagg ccaaaacctt cgagaaggtc atgaatatca atgtcaccag cgtctggcgc ctgatccgtt cggtcgatcc gctgctgcgc gcctcggatg ccgggcgggc gatcatgctc tcctccggtg tggcgcatag ctgccgcgcc ttctgggggc cttatgccgc ctccaaggcc gccgtcgagg ttatggcgcg gtgctgggcc gaagaaacca agaagatgaa gctcaagatc aattcggtca atccgggcgc aacccgcacc gccatgcgcg cgcaggccat gccgggcgaa gacccggaaa cgctccccac gccgcaatcg gtcgccgaaa agatcgtcaa gcttgccgat ccgaaacttg aagtgacggg caagcttttc gacgtgcgcc aggaccgctt cctcgactat cacatgccaa gctaa
Expected Protein Sequence
MAHHHHHHMN IDLTGRLALV TGASRGIGYF LSLELAKRGA HVIAVARTVG GLEELDDEIR KLGSSATLVP LDITDMEALD RLGGTIHERW GKLDILVANA GILGTISPIG HVEAKTFEKV MNINVTSVWR LIRSVDPLLR ASDAGRAIML SSGVAHSCRA FWGPYAASKA AVEVMARCWA EETKKMKLKI NSVNPGATRT AMRAQAMPGE DPETLPTPQS VAEKIVKLAY PKLEVTGKLF DVRQDRFLDY HMPS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaata tcgatcttac gggccgcctc gcgctggtaa cgggcgcatc gcgcggtatt ggctatttcc tttcgcttga actggcaaaa cgcggcgcgc atgtgatcgc cgtcgcccgc acggttggcg gcctggaaga gctggacgac gaaatacgca agcttggcag cagcgccaca ctggttccac tcgatatcac ggatatggaa gccctcgacc gcctcggcgg aaccatccat gaacgctggg gcaagcttga tattctggtg gccaatgccg gcatattggg cacgatttcg cccattggcc atgtcgaggc caaaaccttc gagaaggtca tgaatatcaa tgtcaccagc gtctggcgcc tgatccgttc ggtcgatccg ctgctgcgcg cctcggatgc cgggcgggcg atcatgctct cctccggtgt ggcgcatagc tgccgcgctt tctgggggcc ttatgccgcc tccaaggccg ccgtcgaggt tatggcgcgg tgctgggccg aagaaaccaa aaagatgaag ctcaagatca attcggtcaa tccgggcgca acccgcaccg ccatgcgcgc gcaggccatg ccgggcgaag acccggaaac gctccccacg ccgcaatcgg tcgccgaaaa gatcgtcaag cttgcctatc cgaaacttga agtgacgggc aagcttttcg acgtgcgcca ggaccgcttc ctcgactatc acatgccaag ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat