SSGCID: Target Page

BrovA.01048.a

acyl-CoA dehydrogenase

CENTER ID:	BrovA.01048.a
ORGANISM:	Brucella ovis ATCC 25840
ASSOCIATED DISEASE:	Ovine epididymitis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrovA.01048.a.B1.GE38065	Full length( BrovA.01048.a )		1	244

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	PATRIC_Locus_Tag:VBIBruOvi136990_0227

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRDGDFYILN GTKRFITNAP IADIFTVMAR TAADVKGADG ISAFIVERNS PGLSLGKPDQ KMGQKGALTS DVIFENVRVP ASQLIGGVEG KGFKTAMKVL DKGRLHIAAL STGAAERMLA DTLAYALDRK QFGKPIADFQ LIQAMLADSK AEIYAAKCMV LDAARKRDSG QNVSTEASCA KMFATEMCGR VADRCVQIHG GAGYVSEYAI ERFYRDVRLF RIYEGTTQIQ QIVIARNMIR EARR

NT Sequence

GTGCGCGACG GCGATTTCTA TATTCTCAAC GGAACCAAAC GTTTCATCAC CAATGCGCCG ATCGCGGATA TCTTCACCGT GATGGCCCGC ACGGCGGCGG ATGTCAAAGG GGCAGACGGG ATCAGCGCCT TTATCGTTGA GCGCAACAGC CCCGGCCTTT CGCTTGGCAA GCCTGACCAG AAAATGGGCC AGAAAGGCGC GCTCACCAGC GATGTCATCT TCGAGAATGT CCGGGTTCCG GCCAGCCAGT TGATCGGCGG CGTCGAAGGA AAAGGCTTCA AGACGGCAAT GAAGGTGCTG GACAAGGGAC GCCTGCATAT TGCGGCGCTC AGCACCGGAG CCGCCGAACG TATGCTTGCC GATACGCTGG CCTATGCGCT GGATCGCAAG CAATTCGGCA AGCCGATTGC CGATTTCCAG CTTATTCAAG CCATGCTCGC GGACAGCAAG GCAGAGATTT ATGCCGCCAA ATGCATGGTT CTCGATGCGG CGCGCAAGCG CGACAGCGGG CAGAACGTCA GCACCGAGGC TTCCTGCGCC AAGATGTTTG CAACGGAAAT GTGCGGCCGG GTTGCCGACC GCTGCGTGCA GATACATGGC GGCGCCGGTT ATGTCAGCGA ATATGCGATC GAGCGTTTCT ACCGCGATGT TCGCCTTTTC CGCATTTACG AGGGAACGAC CCAGATCCAG CAGATCGTCA TCGCAAGAAA CATGATCCGC GAAGCCCGGC GC

Details for BrovA.01048.a.B1.GE38065

HARVESTED ON:	5/13/2014
SEQUENCED ON:	5/6/2014
EXPECTED MW:	28kDa
OBSERVED MW:	28kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	97

Validated AA Sequence

MVRDGDFYIL NGTKRFITNA PIADIFTVMA RTAADVKGAD GISAFIVERN SPGLSLGKPD QKMGQKGALT SDVIFENVRV PASQLIGGVE GKGFKTAMKV LDKGRLHIAA LSTGAAERML ADTLAYALDR KQFGKPIADF QLIQAMLADS KAEIYAAKCM VLDAARKRDS GQNVSTEASC AKMFATEMCG RVADRCVQIH GGAGYVSEYA IERFYRDVRL FRIYEGTTQI QQIVIARNMI REARR

Validated NT Sequence

gggnnnnatn atnnncnnnn ggctcaccac caccaccacc atatggtgcg cgacggcgat ttctatattc tcaacggaac caaacgtttc atcaccaatg cgccgatcgc ggatatcttc accgtgatgg cccgcacggc ggcggatgtc aaaggggcag acgggatcag cgcctttatc gttgagcgca acagccccgg cctttcgctt ggcaagcctg accagaaaat gggccagaaa ggcgcgctca ccagcgatgt catcttcgag aatgtccggg ttccggccag ccagttgatc ggcggcgtcg aaggaaaagg cttcaagacg gcaatgaagg tgctggacaa gggacgcctg catattgcgg cgctcagcac cggagccgcc gaacgtatgc ttgccgatac gctggcctat gcgctggatc gcaagcaatt cggcaagccg atcgccgatt tccagcttat tcaagccatg ctcgcggaca gcaaggcaga gatttatgcc gccaaatgca tggttctcga tgcggcgcgc aagcgcgaca gcgggcagaa cgtcagcacc gaggcttcct gcgccaagat gtttgcaacg gaaatgtgcg gccgggttgc cgaccgctgc gtgcagatac atggcggcgc cggttatgtc agcgaatatg cgatcgagcg tttctaccgc gatgttcgcc ttttccgcat ttacgaggga acgacccaga tccagcagat cgtcatcgca agaaacatga tccgcgaagc ccggcgctaa gtgagtaaga taggatccgg ctgctaacaa ggccccgaaa ga

Expected Protein Sequence

MAHHHHHHMR DGDFYILNGT KRFITNAPIA DIFTVMARTA ADVKGADGIS AFIVERNSPG LSLGKPDQKM GQKGALTSDV IFENVRVPAS QLIGGVEGKG FKTAMKVLDK GRLHIAALST GAAERMLADT LAYALDRKQF GKPIADFQLI QAMLADSKAE IYAAKCMVLD AARKRDSGQN VSTEASCAKM FATEMCGRVA DRCVQIHGGA GYVSEYAIER FYRDVRLFRI YEGTTQIQQI VIARNMIREA RR

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgcgcg acggcgattt ctatattctc aacggaacca aacgtttcat caccaatgcg ccgatcgcgg atatcttcac cgtgatggcc cgcacggcgg cggatgtcaa aggggcagac gggatcagcg cctttatcgt tgagcgcaac agccccggcc tttcgcttgg caagcctgac cagaaaatgg gccagaaagg cgcgctcacc agcgatgtca tcttcgagaa tgtccgggtt ccggccagcc agttgatcgg cggcgtcgaa ggaaaaggct tcaagacggc aatgaaggtg ctggacaagg gacgcctgca tattgcggcg ctcagcaccg gagccgccga acgtatgctt gccgatacgc tggcctatgc gctggatcgc aagcaattcg gcaagccgat tgccgatttc cagcttattc aagccatgct cgcggacagc aaggcagaga tttatgccgc caaatgcatg gttctcgatg cggcgcgcaa gcgcgacagc gggcagaacg tcagcaccga ggcttcctgc gccaagatgt ttgcaacgga aatgtgcggc cgggttgccg accgctgcgt gcagatacat ggcggcgccg gttatgtcag cgaatatgcg atcgagcgtt tctaccgcga tgttcgcctt ttccgcattt acgagggaac gacccagatc cagcagatcg tcatcgcaag aaacatgatc cgcgaagccc ggcgctgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat