BrovA.00674.a

Aspartate aminotransferase (EC 2.6.1.1)

CENTER ID: BrovA.00674.a
ORGANISM: Brucella ovis ATCC 25840
ASSOCIATED DISEASE: Ovine epididymitis
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrovA.00674.a.B1.GE36978 Full length( BrovA.00674.a ) 1 387
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruOvi136990_2252
BV-BRC: fig|444178.3.peg.2252
RefSeq: YP_001258888.1
UniProt: A0A0H3AP63

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTTLSNRSAV EPFHAMDVLA EANRRRAAGH PIISMAVGQP ADSAPVVVRQ AAERALKDGR IGYTDALGLI ELREAIAAHY ADHYGVSVSP ERIAVTTGSS AAFNLAFIVY FDPGDRVAIT RPGYPAYRNI LTALGLEVVE IPTGHGTSSS LTAQALAAAH AEKPLKGVLF ASPANPTGAV IDKPELTAII ETARNLGIRV ISDEIYHRLS YEADDVTALE VADDVTIINS FSKYYCMTGW RIGWMVLPDN DIRAIERLSQ SLYISAPELS QRGAIEAFRA TAELERIKDR YRRNRLILLN HLPRMGLDLA APMDGAFYAY CDVSRFTNDS MEFVRGMISE INIAATPGRD FDPLDGHRTV RFSYAGKVEE MEEAVRRLDD WLPQQKA
NT Sequence
TTGACTACTC TTTCCAACCG CAGCGCGGTC GAGCCGTTTC ACGCCATGGA CGTTCTGGCT GAGGCGAACC GTCGGCGCGC GGCGGGCCAT CCGATCATTT CCATGGCGGT TGGCCAACCG GCTGATTCCG CACCTGTGGT CGTTCGTCAG GCCGCAGAGC GCGCGTTGAA AGATGGCCGC ATCGGCTATA CCGACGCTTT GGGCCTGATT GAACTGCGCG AGGCTATCGC CGCGCATTAT GCGGATCATT ACGGCGTCAG CGTGTCGCCG GAGCGGATTG CGGTCACGAC CGGCTCTTCC GCCGCTTTCA ATCTTGCATT TATTGTCTAT TTCGATCCCG GCGACCGGGT GGCGATAACG CGCCCCGGCT ATCCGGCCTA CCGCAACATC TTGACGGCGC TTGGTCTCGA AGTGGTGGAA ATTCCCACCG GCCATGGAAC ATCCTCGTCG CTGACGGCGC AAGCACTTGC CGCCGCCCAT GCTGAAAAAC CCTTGAAGGG CGTTCTCTTT GCAAGCCCCG CCAACCCAAC CGGAGCGGTT ATCGACAAAC CGGAACTGAC GGCGATCATC GAGACGGCAC GTAACCTCGG AATCCGGGTC ATATCGGATG AAATCTATCA TCGCCTGTCC TATGAAGCCG ACGATGTAAC CGCACTTGAA GTTGCCGACG ACGTCACCAT CATCAATTCC TTCTCCAAAT ATTACTGCAT GACGGGCTGG CGCATCGGCT GGATGGTTCT GCCGGACAAC GATATCCGGG CGATTGAGCG CCTCAGCCAG AGCCTCTATA TTTCCGCGCC CGAACTGTCG CAGCGCGGCG CCATTGAGGC CTTCCGCGCC ACTGCGGAAC TGGAACGGAT CAAGGATCGC TATCGCCGAA ACAGGCTTAT CCTGCTCAAT CATTTGCCGC GCATGGGGCT TGATCTAGCT GCACCGATGG ACGGCGCATT CTACGCCTAT TGCGATGTGT CGCGTTTCAC CAATGACAGC ATGGAGTTTG TGCGCGGGAT GATCTCCGAG ATCAATATCG CCGCCACCCC CGGCCGTGAT TTCGATCCGC TGGACGGGCA TCGCACCGTG CGTTTCTCCT ACGCAGGCAA GGTCGAGGAA ATGGAAGAGG CTGTTCGGCG GCTTGACGAC TGGTTGCCGC AGCAGAAAGC G
Details for BrovA.00674.a.B1.GE36978
HARVESTED ON: 5/21/2013
SEQUENCED ON: 5/23/2013
EXPECTED MW: 44kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 83
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHML TTLSNRSAVE PFHAMDVLAE ANRRRAAGHP IISMAVGQPA DSAPVVVRQA AERALKDGRI GYTDALGLIE LREAIAAHYA DHYGVSVSPE RIAVTTGSSA AFNLAFIVYF DPGDRVAITR PGYPAYRNIL TALGLEVVEI PTGHGTFSSL TAQALAAAHA EKPLKGVLFA SPANPTGAVI DKPELTAIIE TARNLGIRVI SDEIYHRLSY EADDVTALEV ADDVTIINSF SKYYCMTGWR IGWMVLPDND IRAIERLSQS LYISAPELSQ RGAXEAFRAT AELERIXDRY RRNXLILLNH LPRMGLDLAA PMDGAXYAXX XVSXXXXXXX XXXGXSXXXI XXXXXXIXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX
Validated NT Sequence
ttttnnttan tttaagaagg aganatacca tggctcacca ccaccaccac catatgttga ctactctttc caaccgcagc gcggtcgagc cgtttcacgc catggacgtt ctggctgagg cgaaccgtcg gcgcgcggcg ggccatccga tcatttccat ggcggttggc caaccggctg attccgcacc tgtggtcgtt cgtcaggccg cagagcgcgc gttgaaagat ggccgcatcg gctataccga cgctttgggc ctgattgaac tgcgcgaggc tatcgccgcg cattatgcgg atcattacgg cgtcagcgtg tcgccggagc ggattgcggt cacgaccggc tcttccgccg ctttcaatct tgcatttatt gtctatttcg atcccggcga ccgggtggcg ataacgcgcc ccggctatcc ggcctaccgc aacatcttga cggcgcttgg tctcgaagtg gtggaaattc ccaccggcca tggaacattc tcgtcgctga cggcgcaagc acttgccgcc gcccatgctg aaaaaccctt gaagggcgtt ctctttgcaa gccccgccaa cccaaccgga gcggttatcg acaaaccgga actgacggcg atcatcgaga cggcacgtaa cctcggaatc cgggtcatat cggatgaaat ctatcatcgc ctgtcctatg aagccgacga tgtaaccgca cttgaagttg ccgacgacgt caccatcatc aattccttct ccaaatatta ctgcatgacg ggctggcgca tcggctggat ggttctgccg gacaacgata tccgggcgat tgagcgcctc agccagagcc tctatatttc cgcgcccgaa ctgtcgcagc gcggcgccnt tgaggccttc cgcgccactg cggaactgga acggatcnnn gatcgctatc gccgaaacng gcttatcctg ctcaatcatc tgccgcgcat ggggcttgat ctggctgcac cgatggacgg cgcntnctac gcctnnnnnn atgtgtcnng ttncnnnnga cngnatgngt tnnnnncngg nngatcncna natnanatcn cnnccnccnc cnnnnngatt cnancnnngn nngnnnnnnn ncnngnnntt cnnnnnnnnn gnnnnnnnng nnnnnnnnnn nnnnnnnnnt nnnnnngcnn nnnnnnnann nnnnnnnnnn na
Expected Protein Sequence
MAHHHHHHMT TLSNRSAVEP FHAMDVLAEA NRRRAAGHPI ISMAVGQPAD SAPVVVRQAA ERALKDGRIG YTDALGLIEL REAIAAHYAD HYGVSVSPER IAVTTGSSAA FNLAFIVYFD PGDRVAITRP GYPAYRNILT ALGLEVVEIP TGHGTSSSLT AQALAAAHAE KPLKGVLFAS PANPTGAVID KPELTAIIET ARNLGIRVIS DEIYHRLSYE ADDVTALEVA DDVTIINSFS KYYCMTGWRI GWMVLPDNDI RAIERLSQSL YISAPELSQR GAIEAFRATA ELERIKDRYR RNRLILLNHL PRMGLDLAAP MDGAFYAYCD VSRFTNDSME FVRGMISEIN IAATPGRDFD PLDGHRTVRF SYAGKVEEME EAVRRLDDWL PQQKA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttgacta ctctttccaa ccgcagcgcg gtcgagccgt ttcacgccat ggacgttctg gctgaggcga accgtcggcg cgcggcgggc catccgatca tttccatggc ggttggccaa ccggctgatt ccgcacctgt ggtcgttcgt caggccgcag agcgcgcgtt gaaagatggc cgcatcggct ataccgacgc tttgggcctg attgaactgc gcgaggctat cgccgcgcat tatgcggatc attacggcgt cagcgtgtcg ccggagcgga ttgcggtcac gaccggctct tccgccgctt tcaatcttgc atttattgtc tatttcgatc ccggcgaccg ggtggcgata acgcgccccg gctatccggc ctaccgcaac atcttgacgg cgcttggtct cgaagtggtg gaaattccca ccggccatgg aacatcctcg tcgctgacgg cgcaagcact tgccgccgcc catgctgaaa aacccttgaa gggcgttctc tttgcaagcc ccgccaaccc aaccggagcg gttatcgaca aaccggaact gacggcgatc atcgagacgg cacgtaacct cggaatccgg gtcatatcgg atgaaatcta tcatcgcctg tcctatgaag ccgacgatgt aaccgcactt gaagttgccg acgacgtcac catcatcaat tccttctcca aatattactg catgacgggc tggcgcatcg gctggatggt tctgccggac aacgatatcc gggcgattga gcgcctcagc cagagcctct atatttccgc gcccgaactg tcgcagcgcg gcgccattga ggccttccgc gccactgcgg aactggaacg gatcaaggat cgctatcgcc gaaacaggct tatcctgctc aatcatttgc cgcgcatggg gcttgatcta gctgcaccga tggacggcgc attctacgcc tattgcgatg tgtcgcgttt caccaatgac agcatggagt ttgtgcgcgg gatgatctcc gagatcaata tcgccgccac ccccggccgt gatttcgatc cgctggacgg gcatcgcacc gtgcgtttct cctacgcagg caaggtcgag gaaatggaag aggctgttcg gcggcttgac gactggttgc cgcagcagaa agcgtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat