BrovA.00232.a
Xylulose kinase (EC 2.7.1.17)
| CENTER ID: | BrovA.00232.a |
| ORGANISM: | Brucella ovis ATCC 25840 |
| ASSOCIATED DISEASE: | Ovine epididymitis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrovA.00232.a.B1.GE38059 | Full length( BrovA.00232.a ) | 1 | 483 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
5VM1
DEPOSITED: 4/26/2017
DETERMINATION: XRay
CLONE: BrovA.00232.a.B1.GE38059
PROTEIN: BrovA.00232.a.B1.PS02131
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|444178.3.peg.1875 |
| RefSeq: | YP_001258549.1 |
| UniProt: | A0A0H3APL8 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MYLGLDLGTS GVKALLIDEA QNPVGAAHGE LDVSRPHPGW SEQDPAQWIK ACRTAIEALR AAHPKEFSAI TGIGLSGQMH GATLLDAEDR VLRPCILWND TRSYREAAEL DADPAFRAIT GNIVFPGFTA PKLVWVARNE ADIFARIRKV LLPKDYLRLW LTGEYISDMS DSAGTSWLDT GARRWSAELL AKTGLGEGQM PQLVEGSEAA GCLRAELAAE WSLTASVIVA GGAGDNAASA CGMGTVKPGH AFVSLGTSGV LFAANGAYQP KPESAVHAFC HALPRTWHQM GVILSAASAL EWYSKIVGAT PQSLDRELGE TLKAPGSVTF LPYLSGERTP YNDAKIRGSF CGLEHEADRS ALTQAVLEGV AFAIRDNLLA LQSAGTEITS LTAVGGGSRS TYWLKAIATA LNVPIALPEE GDFGAAFGAA RLGLIAATGA DPFTICTPPQ TARTIEPEQA LLSAYDEAYQ RYHALYPALH ALD
NT Sequence
ATGTATCTGG GTCTCGATTT GGGAACATCC GGCGTCAAGG CGCTTTTGAT CGATGAGGCG CAGAACCCGG TCGGCGCGGC CCATGGCGAA CTGGACGTTT CGCGCCCGCA TCCCGGCTGG AGCGAACAGG ATCCTGCGCA GTGGATCAAA GCCTGCCGTA CGGCCATAGA AGCCTTGCGC GCGGCGCACC CGAAGGAATT TTCCGCCATT ACCGGTATCG GCCTTTCCGG GCAGATGCAT GGCGCAACCC TGCTGGACGC CGAGGACCGC GTATTGCGCC CCTGCATTCT CTGGAACGAT ACGCGCAGCT ATCGCGAAGC AGCCGAACTG GATGCCGACC CCGCATTTCG CGCCATCACC GGCAATATCG TCTTTCCCGG CTTCACCGCA CCGAAACTTG TCTGGGTCGC ACGCAACGAA GCCGATATTT TCGCCCGCAT CCGCAAGGTT TTACTGCCGA AAGATTATCT GCGTCTCTGG CTCACCGGAG AATATATCTC CGACATGTCG GATTCGGCGG GCACAAGCTG GCTCGATACG GGTGCGCGCC GCTGGTCGGC AGAGCTTCTG GCCAAGACCG GCCTGGGCGA AGGGCAGATG CCGCAACTGG TCGAGGGGAG CGAGGCTGCG GGATGTCTGC GCGCCGAGCT TGCCGCCGAA TGGAGCCTCA CCGCATCGGT AATCGTGGCG GGCGGCGCTG GCGACAATGC GGCTTCCGCC TGCGGCATGG GCACGGTCAA GCCCGGCCAT GCCTTCGTTT CACTTGGCAC TTCGGGCGTG CTTTTCGCTG CCAATGGCGC TTACCAGCCA AAACCGGAAA GTGCGGTCCA TGCCTTCTGC CACGCCCTGC CCCGCACATG GCACCAGATG GGCGTTATCC TGTCTGCGGC AAGCGCGCTT GAATGGTATT CAAAAATCGT TGGCGCAACC CCGCAATCGC TCGATCGGGA GCTGGGCGAA ACCTTGAAAG CACCGGGCAG CGTCACCTTC CTGCCCTATC TTTCAGGCGA GCGCACGCCC TATAATGACG CGAAAATCCG CGGCTCCTTC TGCGGGCTGG AACATGAAGC CGACCGAAGT GCCCTCACCC AGGCCGTGCT GGAAGGCGTC GCCTTTGCCA TTCGCGACAA TCTTCTTGCC CTGCAATCGG CTGGCACCGA GATTACCTCG CTCACCGCAG TCGGTGGCGG ATCACGCTCC ACCTATTGGC TGAAAGCCAT TGCAACAGCA TTGAATGTGC CGATTGCACT GCCCGAAGAA GGCGATTTCG GCGCAGCCTT TGGCGCGGCC CGCCTCGGCC TCATCGCGGC AACCGGGGCC GATCCCTTCA CCATCTGCAC CCCGCCGCAG ACCGCGCGCA CCATTGAGCC GGAACAGGCG CTGCTTTCAG CTTATGACGA AGCCTATCAG CGTTATCACG CGCTTTATCC GGCGCTCCAT GCACTTGAC
Details for BrovA.00232.a.B1.GE38059
| HARVESTED ON: | 5/13/2014 |
| SEQUENCED ON: | 5/6/2014 |
| EXPECTED MW: | 52kDa |
| OBSERVED MW: | 50kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 92 |
| PERCENT COVERAGE: | 55 |
Validated AA Sequence
MSARRACRRM EPHRIGNRGG RRWRQCGFRX GMGTVKPGHA FVSLGTSGVL FAANGAYQPK PESAVHAFCH ALPRTWHQMG VILSAASALE WYSKIVGATP QSLDRELGET LKAPGSVTFL PYLSGERTPY NDAKIRGSFC GLEHEADRSA LTQAVLEGVA FAIRDNLLAL QSAGTEITSL TAVGGGSRST YWLKAIATAL NVPIALPEEG DFGAAFGAAR LGLIAATGAD PFTICTPPQT ARTIEPEQAL LSAYDEAYQR YHALYPALHA LD
Validated NT Sequence
gctnctttcg ggctttgtta gcagccggat cctatcttac tcacttagtc aagtgcatgg agcgccggat aaagcgcgtg ataacgctga taggcttcgt cataagctga aagcagcgcc tgttccggct caatggtgcg cgcggtctgc ggcggggtgc agatggtgaa gggatcggcc ccggttgccg cgatgaggcc gaggcgggcc gcgccaaagg ctgcgccgaa atcgccttct tcgggcagtg caatcggcac attcaatgct gttgcaatgg ctttcagcca ataggtggag cgtgatccgc caccgactgc ggtgagcgag gtaatctcgg tgccagccga ttgcagggca agaagattgt cgcgaatggc aaaggcgacg ccttccagca cggcctgggt gagggcactt cggtcggctt catgttccag cccgcagaag gagccgcgga ttttcgcgtc attatagggc gtgcgctcgc ctgaaagata gggcaggaag gtgacgctgc ccggtgcttt caaggtttcg cccagctccc gatcgagcga ttgcggggtt gcgccaacga tttttgaata ccattcaagc gcgcttgccg cagacaggat aacgcccatc tggtgccatg tgcggggcag ggcgtggcag aaggcatgga ccgcactttc cggttttggc tggtaagcgc cattggcagc gaaaagcacg cccgaagtgc caagtgaaac gaaggcatgg ccgggcttga ccgtgcccat gccgcngcgg aagccgcatt gtcgccagcg ccgcccgcca cgattaccga tgcggtgagg ctccattcgg cggcaagctc ggcgcgcaga catcccgcag cctcgctccc ctcgaccagt tgcggcatct gcccttcgcc caggccggtc ttggccagaa gctctgccga ccagcggcgc gcacccgtat cgagccagct tgtgcccgcc gaatccgaca tgtcgnagat atattctccn ntgagccana gacgcnnata tctttcncag taaaccttgn nntgcggcga nnnncggcnt cntgnngcga ccnnnnantt tcgngcgtna nccggganga cnanttgncg gnnatgannn nangcngggn gcannnngnn tnggcnngcn tcnnnnnnnn ncgacngnnn caananncng tncnngngnn nnnnnnnngg nnnngcgcc
Expected Protein Sequence
MAHHHHHHMY LGLDLGTSGV KALLIDEAQN PVGAAHGELD VSRPHPGWSE QDPAQWIKAC RTAIEALRAA HPKEFSAITG IGLSGQMHGA TLLDAEDRVL RPCILWNDTR SYREAAELDA DPAFRAITGN IVFPGFTAPK LVWVARNEAD IFARIRKVLL PKDYLRLWLT GEYISDMSDS AGTSWLDTGA RRWSAELLAK TGLGEGQMPQ LVEGSEAAGC LRAELAAEWS LTASVIVAGG AGDNAASACG MGTVKPGHAF VSLGTSGVLF AANGAYQPKP ESAVHAFCHA LPRTWHQMGV ILSAASALEW YSKIVGATPQ SLDRELGETL KAPGSVTFLP YLSGERTPYN DAKIRGSFCG LEHEADRSAL TQAVLEGVAF AIRDNLLALQ SAGTEITSLT AVGGGSRSTY WLKAIATALN VPIALPEEGD FGAAFGAARL GLIAATGADP FTICTPPQTA RTIEPEQALL SAYDEAYQRY HALYPALHAL D
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtatc tgggtctcga tttgggaaca tccggcgtca aggcgctttt gatcgatgag gcgcagaacc cggtcggcgc ggcccatggc gaactggacg tttcgcgccc gcatcccggc tggagcgaac aggatcctgc gcagtggatc aaagcctgcc gtacggccat agaagccttg cgcgcggcgc acccgaagga attttccgcc attaccggta tcggcctttc cgggcagatg catggcgcaa ccctgctgga cgccgaggac cgcgtattgc gcccctgcat tctctggaac gatacgcgca gctatcgcga agcagccgaa ctggatgccg accccgcatt tcgcgccatc accggcaata tcgtctttcc cggcttcacc gcaccgaaac ttgtctgggt cgcacgcaac gaagccgata ttttcgcccg catccgcaag gttttactgc cgaaagatta tctgcgtctc tggctcaccg gagaatatat ctccgacatg tcggattcgg cgggcacaag ctggctcgat acgggtgcgc gccgctggtc ggcagagctt ctggccaaga ccggcctggg cgaagggcag atgccgcaac tggtcgaggg gagcgaggct gcgggatgtc tgcgcgccga gcttgccgcc gaatggagcc tcaccgcatc ggtaatcgtg gcgggcggcg ctggcgacaa tgcggcttcc gcctgcggca tgggcacggt caagcccggc catgccttcg tttcacttgg cacttcgggc gtgcttttcg ctgccaatgg cgcttaccag ccaaaaccgg aaagtgcggt ccatgccttc tgccacgccc tgccccgcac atggcaccag atgggcgtta tcctgtctgc ggcaagcgcg cttgaatggt attcaaaaat cgttggcgca accccgcaat cgctcgatcg ggagctgggc gaaaccttga aagcaccggg cagcgtcacc ttcctgccct atctttcagg cgagcgcacg ccctataatg acgcgaaaat ccgcggctcc ttctgcgggc tggaacatga agccgaccga agtgccctca cccaggccgt gctggaaggc gtcgcctttg ccattcgcga caatcttctt gccctgcaat cggctggcac cgagattacc tcgctcaccg cagtcggtgg cggatcacgc tccacctatt ggctgaaagc cattgcaaca gcattgaatg tgccgattgc actgcccgaa gaaggcgatt tcggcgcagc ctttggcgcg gcccgcctcg gcctcatcgc ggcaaccggg gccgatccct tcaccatctg caccccgccg cagaccgcgc gcaccattga gccggaacag gcgctgcttt cagcttatga cgaagcctat cagcgttatc acgcgcttta tccggcgctc catgcacttg actgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat